Re: Experience with Olympus X-Line 100X objective?

Posted by Owens, Peter on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Experience-with-Olympus-X-Line-100X-objective-tp7592261p7592265.html

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I have just purchased a 100x  xline for our facility in NUIG , to be delivered in a few weeks . Interesting to read your comments Alison on the psf and chromatic aberration. Certainly looking forward to trying it out and I can report back to this forum when i have a chance to use it.  I have also heard that Olympus pricing for optics is due to increase by around 20% in June , and this was already a pricey objective.

Best regards

Peter

www.imaging.nuigalway.ie<http://www.imaging.nuigalway.ie>
Centre for Microscopy and Imaging at NUI Galway - current research<http://www.imaging.nuigalway.ie/>
The Centre for Microscopy and Imaging at NUIG (CMI) is a core facility dedicated to providing scientists with a variety of high-end light and electron microscopes along cutting-edge imaging software and analysis tools. Provide expertise in microscopy, imaging and analysis specialising in biomaterials, cell and tissue phenotypes.
www.imaging.nuigalway.ie




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From: Confocal Microscopy List <[hidden email]> on behalf of Alison J. North <[hidden email]>
Sent: 21 May 2021 11:03
To: [hidden email] <[hidden email]>
Subject: Re: Experience with Olympus X-Line 100X objective?

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Hi Abby,

I don't know whether this will be useful information or not, but we recently purchased the 60X X-line objective for our DeltaVision, and I can confirm that the psfs look great and the chromatic aberrations are significantly better than with the original 60x/1.42 oil objective, even when using the Cargille oils (which result in noticeable chromatic dispersion in comparison to when using Olympus oil).  If the 100x X-line objective is anything like as good, then I really should be thinking about buying one myself - with the new 60x we no longer need to do any post-acquisition corrections.

All the best,
Alison

Alison J. North, Ph.D.
Senior Director of the Bio-Imaging Resource Center and Research Associate Professor
The Rockefeller University
1230 York Avenue
New York
NY 10065
Tel. [phone] (office - direct line)
https://www.rockefeller.edu/bioimaging/
RRID:SCR_017791

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From: Confocal Microscopy List <[hidden email]> on behalf of Abby Dernburg <[hidden email]>
Sent: Thursday, May 20, 2021 3:20 PM
To: [hidden email] <[hidden email]>
Subject: Experience with Olympus X-Line 100X objective?

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Hi all -

I am thinking of adding an Olympus X-Line 100X NA 1.45 to our DeltaVision Elite system and am curious about real-world experience with this objective, particularly for wide-field deconvolution microscopy. I haven't found any descirptions of performance other than marketing materials so I'm wondering how it measures up. Thanks in advance for any feedback!

-Abby