Re: Experience with Olympus X-Line 100X objective?

Posted by Abby Dernburg on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Experience-with-Olympus-X-Line-100X-objective-tp7592261p7592268.html

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Thanks to those who responded - now I’m excited to try out the X-line (and thinking I should consider the 60X as well). I didn’t realize that Olympus oil might have been a better choice than the Cargille oils we’ve been using - thanks for that tip, Alison. We do see pretty severe chromatic dispersion in Z with our current 100X objective.

Abby Dernburg, Ph.D.
HHMI Investigator
Department of Molecular and Cell Biology
University of California, Berkeley
Berkeley, CA 94720
USA

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> On May 21, 2021, at 3:30 AM, Owens, Peter <[hidden email]> wrote:
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> I have just purchased a 100x  xline for our facility in NUIG , to be delivered in a few weeks . Interesting to read your comments Alison on the psf and chromatic aberration. Certainly looking forward to trying it out and I can report back to this forum when i have a chance to use it.  I have also heard that Olympus pricing for optics is due to increase by around 20% in June , and this was already a pricey objective.
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> Best regards
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> Peter
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> ________________________________
> From: Confocal Microscopy List <[hidden email]> on behalf of Alison J. North <[hidden email]>
> Sent: 21 May 2021 11:03
> To: [hidden email] <[hidden email]>
> Subject: Re: Experience with Olympus X-Line 100X objective?
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> Hi Abby,
>
> I don't know whether this will be useful information or not, but we recently purchased the 60X X-line objective for our DeltaVision, and I can confirm that the psfs look great and the chromatic aberrations are significantly better than with the original 60x/1.42 oil objective, even when using the Cargille oils (which result in noticeable chromatic dispersion in comparison to when using Olympus oil).  If the 100x X-line objective is anything like as good, then I really should be thinking about buying one myself - with the new 60x we no longer need to do any post-acquisition corrections.
>
> All the best,
> Alison
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> Alison J. North, Ph.D.
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> From: Confocal Microscopy List <[hidden email]> on behalf of Abby Dernburg <[hidden email]>
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> Subject: Experience with Olympus X-Line 100X objective?
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> Hi all -
>
> I am thinking of adding an Olympus X-Line 100X NA 1.45 to our DeltaVision Elite system and am curious about real-world experience with this objective, particularly for wide-field deconvolution microscopy. I haven't found any descirptions of performance other than marketing materials so I'm wondering how it measures up. Thanks in advance for any feedback!
>
> -Abby