Re: Advice on high-end light-sheet

Posted by Armstrong, Brian on Jun 02, 2021; 8:15pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-on-high-end-light-sheet-tp7592328p7592331.html

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Hi, well, a great answer from Zdenek.
The Zeiss Lightsheet 7 can do both large samples (think cleared mouse brain) and small samples (think small transparent organoid), but you need two completely different stage set-ups.
Bruker simply advises you to purchase two separate systems. Maybe a good idea.

Versatility is not the strong point of a lattice light sheet.
Would I like to have one? Sure, however I think it would be difficult to justify based on the number of samples that could be imaged.  

Cheers,

Brian Armstrong PhD
Associate Research Professor
Director, Light Microscopy Core Facility
Beckman Research Institute, City of Hope

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Zdenek Svindrych
Sent: Wednesday, June 02, 2021 12:54 PM
To: [hidden email]
Subject: Re: Advice on high-end light-sheet

[Attention: This email came from an external source. Do not open attachments or click on links from unknown senders or unexpected emails.]

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Versatility?
A good microscope should be able to deal with any sample you throw at it, right?
Not so with a lightsheet microscope. You need to decide whether you need large sample volumes (Lavision Ultramicroscope, Zeiss Lightsheet Z.1 - now apparently rebranded as " Lightsheet 7") or high resolution (3i lattice lightsheet or Zeiss "Lattice Lightsheet 7" - how confusing).
If you want both options, you're going to need two separate instruments...
Absolutely zero commercial interest.
Best, zdenek

On Wed, Jun 2, 2021 at 3:15 PM F Javier Díez Guerra <[hidden email]>
wrote:

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>
> Hi,
>
> We are considering several options for a high-end light-sheet
> (lattice) system and would like to know your experience. The system is
> meant for our advanced light microscopy facility at the CBMSO in
> Madrid, with many users and projects from different fields of the
> biosciences. So we value versatility and robustness, in addition to specifications.
>
> I would be very grateful to listen to your comments off-list (if you
> think it should be that way).
>
> Thanks all for your cooperation.
>
> Best
>
> Javier
>
>
> F Javier Diez-Guerra, PhD
> Servicio de Microscopía Confocal
> Centro de Biologia Molecular Severo Ochoa C/ Nicolás Cabrera, 1 Campus
> de Cantoblanco
> 28049 Madrid
> SPAIN
>
> Tel     [phone]
> e-mail: [hidden email]
>

--
--
Zdenek Svindrych, Ph.D.
Research Scientist - Microscopy Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth

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