Re: Advice on high-end light-sheet

Posted by F Javier Diez Guerra on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-on-high-end-light-sheet-tp7592328p7592334.html

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Thanks Zdenek,

Very clear and sound!

For a better understanding from my side and context, please translate
"versatility" as "accommodation to as many different applications and
user requests as possible". And yes, you are right on the size of the
sample.

As I understand it, most of our users would be more on the gaussian side
of light sheet (bigger samples, embryos, etc), and I was wondering what
would happen with those interested in imaging medium-sized samples, such
as organoids, etc. Lattice light sheet will surely be very kind to
cultured cells and much more resolutive. But for now, we have other
instruments (spinning disk, STED) that cover a wide range of
applications in this category.

As in my earlier email, I'll be very interested and appreciate any
experienced input.

Best

Javier



El 02/06/2021 a las 21:54, Zdenek Svindrych escribió:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Versatility?
> A good microscope should be able to deal with any sample you throw at it,
> right?
> Not so with a lightsheet microscope. You need to decide whether you need
> large sample volumes (Lavision Ultramicroscope, Zeiss Lightsheet Z.1 - now
> apparently rebranded as " Lightsheet 7") or high resolution (3i lattice
> lightsheet or Zeiss "Lattice Lightsheet 7" - how confusing).
> If you want both options, you're going to need two separate instruments...
> Absolutely zero commercial interest.
> Best, zdenek
>
> On Wed, Jun 2, 2021 at 3:15 PM F Javier Díez Guerra <[hidden email]>
> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi,
>>
>> We are considering several options for a high-end light-sheet (lattice)
>> system and would like to know your experience. The system is meant for
>> our advanced light microscopy facility at the CBMSO in Madrid, with many
>> users and projects from different fields of the biosciences. So we value
>> versatility and robustness, in addition to specifications.
>>
>> I would be very grateful to listen to your comments off-list (if you
>> think it should be that way).
>>
>> Thanks all for your cooperation.
>>
>> Best
>>
>> Javier
>>
>>
>> F Javier Diez-Guerra, PhD
>> Servicio de Microscopía Confocal
>> Centro de Biologia Molecular Severo Ochoa
>> C/ Nicolás Cabrera, 1
>> Campus de Cantoblanco
>> 28049 Madrid
>> SPAIN
>>
>> Tel     [phone]
>> e-mail: [hidden email]
>>
>