Re: poly-L-lysine : internal reflection

Posted by CONFOCAL TL Andresen on
URL: http://confocal-microscopy-list.275.s1.nabble.com/poly-L-lysine-internal-reflection-tp787281p787337.html

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Hi Chris

Yes, when you stain with FITC it will also react with poly-lysine. And
you will naturally get huge background fluorescence. You need to use a
fluorofor that does not contain a isothiocyante group (which mean TRITC
is no good either).

Best regards, Thomas

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Chris Katnik
Sent: Wednesday, August 27, 2008 5:12 PM
To: [hidden email]
Subject: poly-L-lysine : internal reflection

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Is there ever a problem with using ploy-L-lysine coated coverslips when
doing
confocal imaging of FITC or Alexa Fluor 555 stained cells and internal
reflection
of the laser light?