fluorophore spectral overlap database for FRET

Posted by John Oreopoulos on
URL: http://confocal-microscopy-list.275.s1.nabble.com/colocalization-analysis-tp786850p839130.html

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I would like to know if there's an online database out there that catalogs the spectral overlap (the J integral) between different fluorophores for FRET imaging. I'm thinking of something similar to the Molecular Probes Java Spectral Viewer that allows you to call up the absorption and emission profile of most of their probes and then you can also superimpose on the graph various laser lines, color filters, etc. It would be great to have something similar that would calculate the J integral for any two FRET pairs.

Thanks.

John Oreopoulos, BSc,

PhD Candidate

University of Toronto

Institute For Biomaterials and Biomedical Engineering

Centre For Studies in Molecular Imaging

Tel: W:416-946-5022