http://confocal-microscopy-list.275.s1.nabble.com/colocalization-analysis-tp786850p841038.html
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> Judy,
>
> colocalization analysis is quite straight forward with ImageJ and the
> "Colocalisation Threshold" plus "Colocalization Test" plugin according to
> Costes.
>
> Regarding triple labeling, which type of questions do you plan to answer?
> I can think about a scenario with two marked structures and how they are
> colocalizing with the nuclei - then you do two runs: nuclei vs. staining
> 1, nuclei vs. staining 2. I am not aware of an established three-color
> colocalization equation. Or do I miss something here?
>
> Michael
>
>
>> Search the CONFOCAL archive at
>>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>>
>> I have a triple labelled sample and would like to do colocalization
>> analysis. What approaches are most people using? The plugins I have seen
>> or used only handle double labelled specimen. A 3-D fluorogram perhaps?
>>
>> Thank you.
>>
>> Judy Trogadis
>> Bio-Imaging Coordinator
>> St. Michael's Hospital, 7Queen
>> 30 Bond St.
>> Toronto, ON M5B 1W8, Canada
>> ph: 416-864-6060 x6337
>> pager: 416-685-9219
>> fax: 416-864-6043
>>
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>