Re: fluorophore spectral overlap database for FRET

Posted by Jean-Pierre CLAMME on
URL: http://confocal-microscopy-list.275.s1.nabble.com/colocalization-analysis-tp786850p842225.html

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Hi,

I'm using http://omlc.ogi.edu/spectra/PhotochemCAD/html/du98.html

It is not a database but It's a good software to calculate spectral
overlap etc. It takes text files so you can add the dyes you are in
interested in.

Hope it will help,

JP


John Oreopoulos wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi listserver,
>
> I would like to know if there's an online database out there that
> catalogs the spectral overlap (the J integral) between different
> fluorophores for FRET imaging. I'm thinking of something similar to
> the Molecular Probes Java Spectral Viewer that allows you to call up
> the absorption and emission profile of most of their probes and then
> you can also superimpose on the graph various laser lines, color
> filters, etc. It would be great to have something similar that would
> calculate the J integral for any two FRET pairs.
>
> Thanks.
>
> John Oreopoulos, BSc,
>
> PhD Candidate
>
> University of Toronto
>
> Institute For Biomaterials and Biomedical Engineering
>
> Centre For Studies in Molecular Imaging
>
>
> Tel: W:416-946-5022
>
>
>