thermal imaging through microscope

[kspencer007]

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Hi;
                I'm looking for suggestions and equipment to do thermal imaging through a microscope. We want to image C. elegans to look at their body temperature under different metabolic conditions. While I know they are poikilotherms, we believe they will show a difference to their local background under these conditions. Can you recommend a decent camera for thermal imaging? Are there IR cutoff filters or optics that will not transmit IR wavelengths in a basic fluorescence-type microscope (needed for worm-sized resolution ~10x mag)?
                Thanks in advance.
                Kathy Spencer

The Scripps Research Institute
Dept of Molecular and Cellular Neuroscience
10550 N. Torrey Pines Road
DNC 216
La Jolla, Ca 92037

[Tobias Baskin]

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Kathy,
          In case you have not seen this, there are people who are
developing ratiometric fluorescence reporters for temperature. For
example: Citation: Nakano M, Arai Y, Kotera I, Okabe K, Kamei Y, Nagai T
(2017) Genetically encoded ratiometric fluorescent thermometer with wide
range and rapid response. PLoS ONE 12(2): e0172344.
doi:10.1371/journal.pone.0172344
      I have no experience with these things, just heard about them and
they seem cool. FYI.

     Tobias

On 10/12/18 2:16 PM, Kathryn Spencer wrote:

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> *****
>
> Hi;
>                  I'm looking for suggestions and equipment to do thermal imaging through a microscope. We want to image C. elegans to look at their body temperature under different metabolic conditions. While I know they are poikilotherms, we believe they will show a difference to their local background under these conditions. Can you recommend a decent camera for thermal imaging? Are there IR cutoff filters or optics that will not transmit IR wavelengths in a basic fluorescence-type microscope (needed for worm-sized resolution ~10x mag)?
>                  Thanks in advance.
>                  Kathy Spencer
>
> The Scripps Research Institute
> Dept of Molecular and Cellular Neuroscience
> 10550 N. Torrey Pines Road
> DNC 216
> La Jolla, Ca 92037
>

--
       __    ___   ^    ___   ___   Tobias I. Baskin
      /  \  /     / \  /      \      Professor  (he)
     /   / /     /   \ \       \      Biology Department
    / __/ /__   /___  \ \       \__    University of Mass.
   /     /     /       \ \       \      611 N. Pleasant St.
  /     /     /         \ \       \      Amherst, Massachusetts
/     /___  /           \ \___/   \_____ USA  01003    413-545-1533
   bio.umass.edu/biology/baskin                       BLOG: blogs.umass.edu/baskin/

[Craig Brideau]

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BK7 optical glass, which is pretty common in microscope optics, drops off
fairly sharply in transmission by 2 to 2.5um, so that will be a problem.
The last time I heard about a lab attempting thermal imaging they had a
custom gold-coated reflective objective constructed for the purpose, and
designed all their other optics with IR glass like zinc selenide or
similar. It seems unlikely this would work at all through a conventional
microscope. I can point you to some additional resources if you are
interested.

Craig

On Fri, Oct 12, 2018 at 12:17 PM Kathryn Spencer <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi;
>                 I'm looking for suggestions and equipment to do thermal
> imaging through a microscope. We want to image C. elegans to look at their
> body temperature under different metabolic conditions. While I know they
> are poikilotherms, we believe they will show a difference to their local
> background under these conditions. Can you recommend a decent camera for
> thermal imaging? Are there IR cutoff filters or optics that will not
> transmit IR wavelengths in a basic fluorescence-type microscope (needed for
> worm-sized resolution ~10x mag)?
>                 Thanks in advance.
>                 Kathy Spencer
>
> The Scripps Research Institute
> Dept of Molecular and Cellular Neuroscience
> 10550 N. Torrey Pines Road
> DNC 216
> La Jolla, Ca 92037
>

[Mark Cannell-2]

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I suggest you do a calculation as to what temperature difference you expect -use the metabolic rate of say reptiles and solve the heat equation for a cylinder of the appropriate size. I suspect the delta t is very very small.

HTH

Mark B. Cannell. Ph.D. FRSNZ FISHR
Department of Physiology, Pharmacology & Neuroscience
School of Medical Sciences
University Walk
Bristol BS8 1TD
 
[hidden email]
 
 

On 12/10/18, 7:17 PM, "Confocal Microscopy List on behalf of Kathryn Spencer" <[hidden email] on behalf of [hidden email]> wrote:

    *****
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    http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    Post images on http://www.imgur.com and include the link in your posting.
    *****
   
    Hi;
                    I'm looking for suggestions and equipment to do thermal imaging through a microscope. We want to image C. elegans to look at their body temperature under different metabolic conditions. While I know they are poikilotherms, we believe they will show a difference to their local background under these conditions. Can you recommend a decent camera for thermal imaging? Are there IR cutoff filters or optics that will not transmit IR wavelengths in a basic fluorescence-type microscope (needed for worm-sized resolution ~10x mag)?
                    Thanks in advance.
                    Kathy Spencer
   
    The Scripps Research Institute
    Dept of Molecular and Cellular Neuroscience
    10550 N. Torrey Pines Road
    DNC 216
    La Jolla, Ca 92037
   

[Sripad Ram-2]

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Have a look at flir cameras (https://www.flir.com/). For reasons mentioned
by Craig, it will not be beneficial to mount the camera on a microscope but
they do have very good sensitivity to detect 0.5 Deg C change.

Sripad


On Fri, Oct 12, 2018 at 1:10 PM Mark Cannell <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I suggest you do a calculation as to what temperature difference you
> expect -use the metabolic rate of say reptiles and solve the heat equation
> for a cylinder of the appropriate size. I suspect the delta t is very very
> small.
>
> HTH
>
> Mark B. Cannell. Ph.D. FRSNZ FISHR
> Department of Physiology, Pharmacology & Neuroscience
> School of Medical Sciences
> University Walk
> Bristol BS8 1TD
>
> [hidden email]
>
>
>
> On 12/10/18, 7:17 PM, "Confocal Microscopy List on behalf of Kathryn
> Spencer" <[hidden email] on behalf of
> [hidden email]> wrote:
>
>     *****
>     To join, leave or search the confocal microscopy listserv, go to:
>     http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>     Post images on http://www.imgur.com and include the link in your
> posting.
>     *****
>
>     Hi;
>                     I'm looking for suggestions and equipment to do
> thermal imaging through a microscope. We want to image C. elegans to look
> at their body temperature under different metabolic conditions. While I
> know they are poikilotherms, we believe they will show a difference to
> their local background under these conditions. Can you recommend a decent
> camera for thermal imaging? Are there IR cutoff filters or optics that will
> not transmit IR wavelengths in a basic fluorescence-type microscope (needed
> for worm-sized resolution ~10x mag)?
>                     Thanks in advance.
>                     Kathy Spencer
>
>     The Scripps Research Institute
>     Dept of Molecular and Cellular Neuroscience
>     10550 N. Torrey Pines Road
>     DNC 216
>     La Jolla, Ca 92037
>
>
>

[Karel Zuzak]

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Dear Kathy,

I am not aware of any commercially available thermal microscope but that doesn’t mean it doesn’t exist.

While at NIH In the late 90s our lab was exploring various military technologies for medical application and thermal imaging was one.

As you might imagine there have been great advances in thermal imaging as well as NIR based night vision. The advantage of thermal is that it is passive. Meaning one does not need a source. Your subject is the source. Unlike fluorescence or NIR where one must expose the subject to an excitation wavelength or NIR illumination.

So thermal is great but we found a lot of subject to subject variability in temperature which may have been from the ambient surrounding or one of the many physiologic factors influencing temperature.

The other difficulty with thermal was the need for expensive custom optics.

So please know once you get a thermal system working you may encounter a lot of variability from your experiments that will require a lot of control to separate the potentially different sources to metabolism.  

Regarding the optics it’s a question of your budget. Back in the 1990s a system could easily cost over 100k for the camera alone and needed constant liquid nitrogen. The reason being thermal emission occurs around 7 to 14 um. Assuming I’m remembering correctly. A standard ccd or cmos detector won’t detect these wavelengths and standard glass optics won’t transmit those wavelengths sufficiently. So one must use specialized focal plane arrays and optics along with imaging methods using long integration or exposure times or co-adds etc. Then if you need greater magnification and need microscope optics then you need to be sure all the optics in that microscope light path will transmit the thermal wavelengths.  

That said great advances have been made in the thermal field. One can pick up a point/shoot IR thermometer at the hardware store.  As an example  the cost of consumer thermal imaging scopes for hunting applications are down to 5k where previously they could cost up around 80k. I have seen some  as low as 700 but the image resolution and thermal variability reflect that price.  

So if you are set on doing this I would suggest knowing your continuum; the thermal/temperature and imaging sensitivity and resolution. Then I would start with a call to FLIR. They make a variety of thermal and NIR cameras and can possibly help you out. Another place you might like to call are microscope companies such as Zeiss, Nikon, Olympus etc and see what they have to offer.

If after all this you are still interested and have the budget. I’m happy to work with you toward finding/developing a custom solution.  

Warm Regards
Karel Zuzak
[hidden email]
[hidden email]  

🤠


On Oct 12, 2018, at 3:05 PM, Craig Brideau <[hidden email]> wrote:

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BK7 optical glass, which is pretty common in microscope optics, drops off
fairly sharply in transmission by 2 to 2.5um, so that will be a problem.
The last time I heard about a lab attempting thermal imaging they had a
custom gold-coated reflective objective constructed for the purpose, and
designed all their other optics with IR glass like zinc selenide or
similar. It seems unlikely this would work at all through a conventional
microscope. I can point you to some additional resources if you are
interested.

Craig

On Fri, Oct 12, 2018 at 12:17 PM Kathryn Spencer <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi;
>                I'm looking for suggestions and equipment to do thermal
> imaging through a microscope. We want to image C. elegans to look at their
> body temperature under different metabolic conditions. While I know they
> are poikilotherms, we believe they will show a difference to their local
> background under these conditions. Can you recommend a decent camera for
> thermal imaging? Are there IR cutoff filters or optics that will not
> transmit IR wavelengths in a basic fluorescence-type microscope (needed for
> worm-sized resolution ~10x mag)?
>                Thanks in advance.
>                Kathy Spencer
>
> The Scripps Research Institute
> Dept of Molecular and Cellular Neuroscience
> 10550 N. Torrey Pines Road
> DNC 216
> La Jolla, Ca 92037
>

[Dr. K N Ganesh]

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I have seen Stage to CO2 incubator companies publish data of temperature gradients on the 96 well plate precisely
Ganesh

Sent from my iPhone

> On 13-Oct-2018, at 10:08 AM, Karel Zuzak <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Kathy,
>
> I am not aware of any commercially available thermal microscope but that doesn’t mean it doesn’t exist.
>
> While at NIH In the late 90s our lab was exploring various military technologies for medical application and thermal imaging was one.
>
> As you might imagine there have been great advances in thermal imaging as well as NIR based night vision. The advantage of thermal is that it is passive. Meaning one does not need a source. Your subject is the source. Unlike fluorescence or NIR where one must expose the subject to an excitation wavelength or NIR illumination.
>
> So thermal is great but we found a lot of subject to subject variability in temperature which may have been from the ambient surrounding or one of the many physiologic factors influencing temperature.
>
> The other difficulty with thermal was the need for expensive custom optics.
>
> So please know once you get a thermal system working you may encounter a lot of variability from your experiments that will require a lot of control to separate the potentially different sources to metabolism.  
>
> Regarding the optics it’s a question of your budget. Back in the 1990s a system could easily cost over 100k for the camera alone and needed constant liquid nitrogen. The reason being thermal emission occurs around 7 to 14 um. Assuming I’m remembering correctly. A standard ccd or cmos detector won’t detect these wavelengths and standard glass optics won’t transmit those wavelengths sufficiently. So one must use specialized focal plane arrays and optics along with imaging methods using long integration or exposure times or co-adds etc. Then if you need greater magnification and need microscope optics then you need to be sure all the optics in that microscope light path will transmit the thermal wavelengths.  
>
> That said great advances have been made in the thermal field. One can pick up a point/shoot IR thermometer at the hardware store.  As an example  the cost of consumer thermal imaging scopes for hunting applications are down to 5k where previously they could cost up around 80k. I have seen some  as low as 700 but the image resolution and thermal variability reflect that price.  
>
> So if you are set on doing this I would suggest knowing your continuum; the thermal/temperature and imaging sensitivity and resolution. Then I would start with a call to FLIR. They make a variety of thermal and NIR cameras and can possibly help you out. Another place you might like to call are microscope companies such as Zeiss, Nikon, Olympus etc and see what they have to offer.
>
> If after all this you are still interested and have the budget. I’m happy to work with you toward finding/developing a custom solution.  
>
> Warm Regards
> Karel Zuzak
> [hidden email]
> [hidden email]  
>
> 🤠
>
>
> On Oct 12, 2018, at 3:05 PM, Craig Brideau <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> BK7 optical glass, which is pretty common in microscope optics, drops off
> fairly sharply in transmission by 2 to 2.5um, so that will be a problem.
> The last time I heard about a lab attempting thermal imaging they had a
> custom gold-coated reflective objective constructed for the purpose, and
> designed all their other optics with IR glass like zinc selenide or
> similar. It seems unlikely this would work at all through a conventional
> microscope. I can point you to some additional resources if you are
> interested.
>
> Craig
>
> On Fri, Oct 12, 2018 at 12:17 PM Kathryn Spencer <[hidden email]>
> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi;
>>               I'm looking for suggestions and equipment to do thermal
>> imaging through a microscope. We want to image C. elegans to look at their
>> body temperature under different metabolic conditions. While I know they
>> are poikilotherms, we believe they will show a difference to their local
>> background under these conditions. Can you recommend a decent camera for
>> thermal imaging? Are there IR cutoff filters or optics that will not
>> transmit IR wavelengths in a basic fluorescence-type microscope (needed for
>> worm-sized resolution ~10x mag)?
>>               Thanks in advance.
>>               Kathy Spencer
>>
>> The Scripps Research Institute
>> Dept of Molecular and Cellular Neuroscience
>> 10550 N. Torrey Pines Road
>> DNC 216
>> La Jolla, Ca 92037
>>

[Benjamin Smith]

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*****

One thing no one has brought up yet is the emissivity problem.  Thermal
imaging works only if the object has high emissivity (i.e. black in the IR
spectrum).  For example, put a piece of black electrical tape on a piece of
aluminum foil and heat the foil up to 100°C (use a thermocouple to confirm
the temperature).  If you point an IR thermometer at the electrical tape,
you will get a reading of 100°C, but if you point the IR thermometer at the
aluminum, you will get a temperature of 22°C.  This is because aluminum is
highly reflective, meaning that it will emit very little IR when heated (it
has 1/25th the emissivity of the electrical tape).  As such, the faint IR
emitted from the foil is swamped out by the much brighter IR being emitted
from nearby objects with higher emissivity, which them reflects off of the
aluminum foil, giving you the reading of 22°C (or whatever the ambient
temperature is).  The point is that the amount of IR something emits does
not correlate to its temperature, due to different objects having different
emissivities.  Along these lines, if someone wrapped themselves in a space
blanket, they would become effectively invisible to an IR camera.  Here is
a nice article explaining the emissivity problem with images:
https://crimsoniv.co.uk/thermography-tips-n-tricks-1-emissivity-estimate/

That said, water has a relatively high emissivity, but here in lies the
problem.  The water around the nematode will be emitting almost exactly the
same amount of IR as the nematode itself, and even in the same spectrum (as
the temperatures are effectively identical).  This is like trying to image
a FITC stained sample that is immersed in a concentrated fluorescein
solution.  Conversely, if you removed all of the surrounding water and
placed the nematode on a piece of aluminum foil, you would have a
reasonable shot at using IR to measure the temperature of the nematode, but
I doubt they would survive very long.  The only way I could begin to think
of how to make this work would be to have a reflective optics microscope
(as mentioned by Craig) and place the nematodes on a polished aluminum
block with a thin film of water on top, such that there is minimal water
above and below the nematode.

A few practical/economical solutions that come to mind:
1) Place a fine guage thermocouple adjacent to the nematode:
http://www.twire.co.jp/english/Thermocouple2-en.html
2) To enhance the signal in #1, place the nematode in a micro-scale
calorimeter.
3) Use the proteins mentioned by Tobias.

Ideally, I would use all three ideas, and a bit of computational modelling
and calibration, such that you can get independent verification of the
result.

Hope this helps,
   Ben Smith

P.S.
   With all this talk of putting on aluminum foil to avoid IR cameras, a
certain Al Yankovic song comes to mind:
https://www.youtube.com/watch?v=urglg3WimHA&feature=youtu.be&t=109

On Fri, Oct 12, 2018 at 9:49 PM Karel Zuzak <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Kathy,
>
> I am not aware of any commercially available thermal microscope but that
> doesn’t mean it doesn’t exist.
>
> While at NIH In the late 90s our lab was exploring various military
> technologies for medical application and thermal imaging was one.
>
> As you might imagine there have been great advances in thermal imaging as
> well as NIR based night vision. The advantage of thermal is that it is
> passive. Meaning one does not need a source. Your subject is the source.
> Unlike fluorescence or NIR where one must expose the subject to an
> excitation wavelength or NIR illumination.
>
> So thermal is great but we found a lot of subject to subject variability
> in temperature which may have been from the ambient surrounding or one of
> the many physiologic factors influencing temperature.
>
> The other difficulty with thermal was the need for expensive custom
> optics.
>
> So please know once you get a thermal system working you may encounter a
> lot of variability from your experiments that will require a lot of control
> to separate the potentially different sources to metabolism.
>
> Regarding the optics it’s a question of your budget. Back in the 1990s a
> system could easily cost over 100k for the camera alone and needed constant
> liquid nitrogen. The reason being thermal emission occurs around 7 to 14
> um. Assuming I’m remembering correctly. A standard ccd or cmos detector
> won’t detect these wavelengths and standard glass optics won’t transmit
> those wavelengths sufficiently. So one must use specialized focal plane
> arrays and optics along with imaging methods using long integration or
> exposure times or co-adds etc. Then if you need greater magnification and
> need microscope optics then you need to be sure all the optics in that
> microscope light path will transmit the thermal wavelengths.
>
> That said great advances have been made in the thermal field. One can pick
> up a point/shoot IR thermometer at the hardware store.  As an example  the
> cost of consumer thermal imaging scopes for hunting applications are down
> to 5k where previously they could cost up around 80k. I have seen some  as
> low as 700 but the image resolution and thermal variability reflect that
> price.
>
> So if you are set on doing this I would suggest knowing your continuum;
> the thermal/temperature and imaging sensitivity and resolution. Then I
> would start with a call to FLIR. They make a variety of thermal and NIR
> cameras and can possibly help you out. Another place you might like to call
> are microscope companies such as Zeiss, Nikon, Olympus etc and see what
> they have to offer.
>
> If after all this you are still interested and have the budget. I’m happy
> to work with you toward finding/developing a custom solution.
>
> Warm Regards
> Karel Zuzak
> [hidden email]
> [hidden email]
>
> 🤠
>
>
> On Oct 12, 2018, at 3:05 PM, Craig Brideau <[hidden email]>
> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> BK7 optical glass, which is pretty common in microscope optics, drops off
> fairly sharply in transmission by 2 to 2.5um, so that will be a problem.
> The last time I heard about a lab attempting thermal imaging they had a
> custom gold-coated reflective objective constructed for the purpose, and
> designed all their other optics with IR glass like zinc selenide or
> similar. It seems unlikely this would work at all through a conventional
> microscope. I can point you to some additional resources if you are
> interested.
>
> Craig
>
> On Fri, Oct 12, 2018 at 12:17 PM Kathryn Spencer <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi;
> >                I'm looking for suggestions and equipment to do thermal
> > imaging through a microscope. We want to image C. elegans to look at
> their
> > body temperature under different metabolic conditions. While I know they
> > are poikilotherms, we believe they will show a difference to their local
> > background under these conditions. Can you recommend a decent camera for
> > thermal imaging? Are there IR cutoff filters or optics that will not
> > transmit IR wavelengths in a basic fluorescence-type microscope (needed
> for
> > worm-sized resolution ~10x mag)?
> >                Thanks in advance.
> >                Kathy Spencer
> >
> > The Scripps Research Institute
> > Dept of Molecular and Cellular Neuroscience
> > 10550 N. Torrey Pines Road
> > DNC 216
> > La Jolla, Ca 92037
> >
>

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Life Sciences Addition
Berkeley, CA  94720-3200
Tel  [phone]
Fax [phone]
e-mail: [hidden email]
http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>

[Benjamin Smith]

*****
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*****

A quick websearch also showed that at the very least, you wouldn't have to
make your own microscope: https://www.optotherm.com/micro-intro.htm

You could always ask for a demo and see if it works.  They have an
objective with 5um per pixel resolution, and the camera has a
noise-equivalent temperature of <50mK so it should be able to catch 0.5°C
temperature changes, especially if multiple frames are averaged together.

Cheers,
   Ben Smith

On Fri, Oct 12, 2018 at 10:44 PM Ganesh Kadasoor <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I have seen Stage to CO2 incubator companies publish data of temperature
> gradients on the 96 well plate precisely
> Ganesh
>
> Sent from my iPhone
>
> > On 13-Oct-2018, at 10:08 AM, Karel Zuzak <[hidden email]> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear Kathy,
> >
> > I am not aware of any commercially available thermal microscope but that
> doesn’t mean it doesn’t exist.
> >
> > While at NIH In the late 90s our lab was exploring various military
> technologies for medical application and thermal imaging was one.
> >
> > As you might imagine there have been great advances in thermal imaging
> as well as NIR based night vision. The advantage of thermal is that it is
> passive. Meaning one does not need a source. Your subject is the source.
> Unlike fluorescence or NIR where one must expose the subject to an
> excitation wavelength or NIR illumination.
> >
> > So thermal is great but we found a lot of subject to subject variability
> in temperature which may have been from the ambient surrounding or one of
> the many physiologic factors influencing temperature.
> >
> > The other difficulty with thermal was the need for expensive custom
> optics.
> >
> > So please know once you get a thermal system working you may encounter a
> lot of variability from your experiments that will require a lot of control
> to separate the potentially different sources to metabolism.
> >
> > Regarding the optics it’s a question of your budget. Back in the 1990s a
> system could easily cost over 100k for the camera alone and needed constant
> liquid nitrogen. The reason being thermal emission occurs around 7 to 14
> um. Assuming I’m remembering correctly. A standard ccd or cmos detector
> won’t detect these wavelengths and standard glass optics won’t transmit
> those wavelengths sufficiently. So one must use specialized focal plane
> arrays and optics along with imaging methods using long integration or
> exposure times or co-adds etc. Then if you need greater magnification and
> need microscope optics then you need to be sure all the optics in that
> microscope light path will transmit the thermal wavelengths.
> >
> > That said great advances have been made in the thermal field. One can
> pick up a point/shoot IR thermometer at the hardware store.  As an example
> the cost of consumer thermal imaging scopes for hunting applications are
> down to 5k where previously they could cost up around 80k. I have seen
> some  as low as 700 but the image resolution and thermal variability
> reflect that price.
> >
> > So if you are set on doing this I would suggest knowing your continuum;
> the thermal/temperature and imaging sensitivity and resolution. Then I
> would start with a call to FLIR. They make a variety of thermal and NIR
> cameras and can possibly help you out. Another place you might like to call
> are microscope companies such as Zeiss, Nikon, Olympus etc and see what
> they have to offer.
> >
> > If after all this you are still interested and have the budget. I’m
> happy to work with you toward finding/developing a custom solution.
> >
> > Warm Regards
> > Karel Zuzak
> > [hidden email]
> > [hidden email]
> >
> > 🤠
> >
> >
> > On Oct 12, 2018, at 3:05 PM, Craig Brideau <[hidden email]>
> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > BK7 optical glass, which is pretty common in microscope optics, drops off
> > fairly sharply in transmission by 2 to 2.5um, so that will be a problem.
> > The last time I heard about a lab attempting thermal imaging they had a
> > custom gold-coated reflective objective constructed for the purpose, and
> > designed all their other optics with IR glass like zinc selenide or
> > similar. It seems unlikely this would work at all through a conventional
> > microscope. I can point you to some additional resources if you are
> > interested.
> >
> > Craig
> >
> > On Fri, Oct 12, 2018 at 12:17 PM Kathryn Spencer <[hidden email]>
> > wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> Post images on http://www.imgur.com and include the link in your
> posting.
> >> *****
> >>
> >> Hi;
> >>               I'm looking for suggestions and equipment to do thermal
> >> imaging through a microscope. We want to image C. elegans to look at
> their
> >> body temperature under different metabolic conditions. While I know they
> >> are poikilotherms, we believe they will show a difference to their local
> >> background under these conditions. Can you recommend a decent camera for
> >> thermal imaging? Are there IR cutoff filters or optics that will not
> >> transmit IR wavelengths in a basic fluorescence-type microscope (needed
> for
> >> worm-sized resolution ~10x mag)?
> >>               Thanks in advance.
> >>               Kathy Spencer
> >>
> >> The Scripps Research Institute
> >> Dept of Molecular and Cellular Neuroscience
> >> 10550 N. Torrey Pines Road
> >> DNC 216
> >> La Jolla, Ca 92037
> >>
>

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Life Sciences Addition
Berkeley, CA  94720-3200
Tel  [phone]
Fax [phone]
e-mail: [hidden email]
http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>

[Duchen, Michael]

*****
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Greetings. The other thing to think about might be 'molecular thermometers' - genetically encoded temperature sensitive probes. Several have been generated although I have to say that our attempts to use these to explore mitochondrial temperature have proven far more difficult and complex than the literature would suggest.

worth thinking about though.


Green, fast, and large-scale synthesis of highly fluorescent Au nanoclusters for Cu2+ detection and temperature sensing.<https://www.ncbi.nlm.nih.gov/pubmed/30246811>

Jia Y, Sun T, Jiang Y, Sun W, Zhao Y, Xin J, Hou Y, Yang W.

Analyst. 2018 Sep 24. doi: 10.1039/c8an01617h.


In situ temperature monitoring in single-molecule FRET experiments.<https://www.ncbi.nlm.nih.gov/pubmed/29604846>

Hartmann A, Berndt F, Ollmann S, Krainer G, Schlierf M.

J Chem Phys. 2018 Mar 28;148(12):123330


Mitochondria are physiologically maintained at close to 50 °C.<https://www.ncbi.nlm.nih.gov/pubmed/29370167>

Chrétien D, Bénit P, Ha HH, Keipert S, El-Khoury R, Chang YT, Jastroch M, Jacobs HT, Rustin P, Rak M.

PLoS Biol. 2018 Jan 25;16(1):e2003992.


and there are quite a few others along these lines.


best wishes

Michael Duchen




________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Karel Zuzak <[hidden email]>
Sent: 13 October 2018 05:38
To: [hidden email]
Subject: Re: thermal imaging through microscope

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Post images on http://www.imgur.com and include the link in your posting.
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Dear Kathy,

I am not aware of any commercially available thermal microscope but that doesn’t mean it doesn’t exist.

While at NIH In the late 90s our lab was exploring various military technologies for medical application and thermal imaging was one.

As you might imagine there have been great advances in thermal imaging as well as NIR based night vision. The advantage of thermal is that it is passive. Meaning one does not need a source. Your subject is the source. Unlike fluorescence or NIR where one must expose the subject to an excitation wavelength or NIR illumination.

So thermal is great but we found a lot of subject to subject variability in temperature which may have been from the ambient surrounding or one of the many physiologic factors influencing temperature.

The other difficulty with thermal was the need for expensive custom optics.

So please know once you get a thermal system working you may encounter a lot of variability from your experiments that will require a lot of control to separate the potentially different sources to metabolism.

Regarding the optics it’s a question of your budget. Back in the 1990s a system could easily cost over 100k for the camera alone and needed constant liquid nitrogen. The reason being thermal emission occurs around 7 to 14 um. Assuming I’m remembering correctly. A standard ccd or cmos detector won’t detect these wavelengths and standard glass optics won’t transmit those wavelengths sufficiently. So one must use specialized focal plane arrays and optics along with imaging methods using long integration or exposure times or co-adds etc. Then if you need greater magnification and need microscope optics then you need to be sure all the optics in that microscope light path will transmit the thermal wavelengths.

That said great advances have been made in the thermal field. One can pick up a point/shoot IR thermometer at the hardware store.  As an example  the cost of consumer thermal imaging scopes for hunting applications are down to 5k where previously they could cost up around 80k. I have seen some  as low as 700 but the image resolution and thermal variability reflect that price.

So if you are set on doing this I would suggest knowing your continuum; the thermal/temperature and imaging sensitivity and resolution. Then I would start with a call to FLIR. They make a variety of thermal and NIR cameras and can possibly help you out. Another place you might like to call are microscope companies such as Zeiss, Nikon, Olympus etc and see what they have to offer.

If after all this you are still interested and have the budget. I’m happy to work with you toward finding/developing a custom solution.

Warm Regards
Karel Zuzak
[hidden email]
[hidden email]

🤠


On Oct 12, 2018, at 3:05 PM, Craig Brideau <[hidden email]> wrote:

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

BK7 optical glass, which is pretty common in microscope optics, drops off
fairly sharply in transmission by 2 to 2.5um, so that will be a problem.
The last time I heard about a lab attempting thermal imaging they had a
custom gold-coated reflective objective constructed for the purpose, and
designed all their other optics with IR glass like zinc selenide or
similar. It seems unlikely this would work at all through a conventional
microscope. I can point you to some additional resources if you are
interested.

Craig

On Fri, Oct 12, 2018 at 12:17 PM Kathryn Spencer <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi;
>                I'm looking for suggestions and equipment to do thermal
> imaging through a microscope. We want to image C. elegans to look at their
> body temperature under different metabolic conditions. While I know they
> are poikilotherms, we believe they will show a difference to their local
> background under these conditions. Can you recommend a decent camera for
> thermal imaging? Are there IR cutoff filters or optics that will not
> transmit IR wavelengths in a basic fluorescence-type microscope (needed for
> worm-sized resolution ~10x mag)?
>                Thanks in advance.
>                Kathy Spencer
>
> The Scripps Research Institute
> Dept of Molecular and Cellular Neuroscience
> 10550 N. Torrey Pines Road
> DNC 216
> La Jolla, Ca 92037
>

[Masur, Sandra]

*****
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*****

Direct thermal imaging references:


1.         Itoh H, Arai S, Sudhaharan T, Lee S-C, Chang Y-T, Ishiwata Si, et al. Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes. Chemical Communications. 2016;52(24):4458-61.

2.         Arai S, Suzuki M, Park S-J, Yoo JS, Wang L, Kang N-Y, et al. Mitochondria-targeted fluorescent thermometer monitors intracellular temperature gradient. Chemical Communications. 2015;51(38):8044-7.

3.         Arai S, Lee S-C, Zhai D, Suzuki M, and Chang YT. A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum. Scientific reports. 2014;4:6701.

4.         Hayashi T, Fukuda N, Uchiyama S, and Inada N. A cell-permeable fluorescent polymeric thermometer for intracellular temperature mapping in mammalian cell lines. PloS one. 2015;10(2):e0117677.

5.         Kiyonaka S, Kajimoto T, Sakaguchi R, Shinmi D, Omatsu-Kanbe M, Matsuura H, et al. Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells. Nature methods. 2013;10(12):1232-8.

HTH

Sandy
__________________________
Sandra K. Masur, PhD

Professor, Ophthalmology
Icahn School of Medicine at Mount Sinai,
Box 1183
1 Gustave Levy Place
New York NY 10029-6574

[hidden email]<mailto:[hidden email]>
telephone: [phone]
fax: [phone]
cell: 646-245-5934

email: [hidden email]


On Oct 12, 2018, at 4:10 PM, Mark Cannell <[hidden email]> wrote:

*****
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Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=4z96eBNtgIwM1gMIdorhq6F2qVwh1ptB6zZyu4OxdcI&m=djMG4hE4hqAqXigEOv82HcHoIGCd1_A5RjvzPEXfCUA&s=DzyTOvYGbyl7EPPE-AMGFonC1imhm5IkscXHIKPSuew&e= and include the link in your posting.
*****

I suggest you do a calculation as to what temperature difference you expect -use the metabolic rate of say reptiles and solve the heat equation for a cylinder of the appropriate size. I suspect the delta t is very very small.

HTH

Mark B. Cannell. Ph.D. FRSNZ FISHR
Department of Physiology, Pharmacology & Neuroscience
School of Medical Sciences
University Walk
Bristol BS8 1TD

[hidden email]



On 12/10/18, 7:17 PM, "Confocal Microscopy List on behalf of Kathryn Spencer" <[hidden email] on behalf of [hidden email]> wrote:

   *****
   To join, leave or search the confocal microscopy listserv, go to:
   https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=4z96eBNtgIwM1gMIdorhq6F2qVwh1ptB6zZyu4OxdcI&m=djMG4hE4hqAqXigEOv82HcHoIGCd1_A5RjvzPEXfCUA&s=gVwGP7nE8gUcKZPkW72tAVO-fCBxlZfmESL_PfLy3IY&e=
   Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=4z96eBNtgIwM1gMIdorhq6F2qVwh1ptB6zZyu4OxdcI&m=djMG4hE4hqAqXigEOv82HcHoIGCd1_A5RjvzPEXfCUA&s=DzyTOvYGbyl7EPPE-AMGFonC1imhm5IkscXHIKPSuew&e= and include the link in your posting.
   *****

   Hi;
                   I'm looking for suggestions and equipment to do thermal imaging through a microscope. We want to image C. elegans to look at their body temperature under different metabolic conditions. While I know they are poikilotherms, we believe they will show a difference to their local background under these conditions. Can you recommend a decent camera for thermal imaging? Are there IR cutoff filters or optics that will not transmit IR wavelengths in a basic fluorescence-type microscope (needed for worm-sized resolution ~10x mag)?
                   Thanks in advance.
                   Kathy Spencer

   The Scripps Research Institute
   Dept of Molecular and Cellular Neuroscience
   10550 N. Torrey Pines Road
   DNC 216
   La Jolla, Ca 92037

[0000001ed7f52e4a-dmarc-request]

*****
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*****

Another exotic idea, comparing stokes and anti-stokes Raman intensities and derive temperature from this? I heard that this is used for combustion engines, but not sure how sensitive and if the fish will survive the high laser powers.

Best wishes

Andreas

Sent from my phone

> On 13 Oct 2018, at 13:50, Masur, Sandra <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Direct thermal imaging references:
>
>
> 1.         Itoh H, Arai S, Sudhaharan T, Lee S-C, Chang Y-T, Ishiwata Si, et al. Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes. Chemical Communications. 2016;52(24):4458-61.
>
> 2.         Arai S, Suzuki M, Park S-J, Yoo JS, Wang L, Kang N-Y, et al. Mitochondria-targeted fluorescent thermometer monitors intracellular temperature gradient. Chemical Communications. 2015;51(38):8044-7.
>
> 3.         Arai S, Lee S-C, Zhai D, Suzuki M, and Chang YT. A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum. Scientific reports. 2014;4:6701.
>
> 4.         Hayashi T, Fukuda N, Uchiyama S, and Inada N. A cell-permeable fluorescent polymeric thermometer for intracellular temperature mapping in mammalian cell lines. PloS one. 2015;10(2):e0117677.
>
> 5.         Kiyonaka S, Kajimoto T, Sakaguchi R, Shinmi D, Omatsu-Kanbe M, Matsuura H, et al. Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells. Nature methods. 2013;10(12):1232-8.
>
> HTH
>
> Sandy
> __________________________
> Sandra K. Masur, PhD
>
> Professor, Ophthalmology
> Icahn School of Medicine at Mount Sinai,
> Box 1183
> 1 Gustave Levy Place
> New York NY 10029-6574
>
> [hidden email]<mailto:[hidden email]>
> telephone: [phone]
> fax: [phone]
> cell: 646-245-5934
>
> email: [hidden email]
>
>
> On Oct 12, 2018, at 4:10 PM, Mark Cannell <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=4z96eBNtgIwM1gMIdorhq6F2qVwh1ptB6zZyu4OxdcI&m=djMG4hE4hqAqXigEOv82HcHoIGCd1_A5RjvzPEXfCUA&s=gVwGP7nE8gUcKZPkW72tAVO-fCBxlZfmESL_PfLy3IY&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=4z96eBNtgIwM1gMIdorhq6F2qVwh1ptB6zZyu4OxdcI&m=djMG4hE4hqAqXigEOv82HcHoIGCd1_A5RjvzPEXfCUA&s=DzyTOvYGbyl7EPPE-AMGFonC1imhm5IkscXHIKPSuew&e= and include the link in your posting.
> *****
>
> I suggest you do a calculation as to what temperature difference you expect -use the metabolic rate of say reptiles and solve the heat equation for a cylinder of the appropriate size. I suspect the delta t is very very small.
>
> HTH
>
> Mark B. Cannell. Ph.D. FRSNZ FISHR
> Department of Physiology, Pharmacology & Neuroscience
> School of Medical Sciences
> University Walk
> Bristol BS8 1TD
>
> [hidden email]
>
>
>
> On 12/10/18, 7:17 PM, "Confocal Microscopy List on behalf of Kathryn Spencer" <[hidden email] on behalf of [hidden email]> wrote:
>
>   *****
>   To join, leave or search the confocal microscopy listserv, go to:
>   https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=4z96eBNtgIwM1gMIdorhq6F2qVwh1ptB6zZyu4OxdcI&m=djMG4hE4hqAqXigEOv82HcHoIGCd1_A5RjvzPEXfCUA&s=gVwGP7nE8gUcKZPkW72tAVO-fCBxlZfmESL_PfLy3IY&e=
>   Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=4z96eBNtgIwM1gMIdorhq6F2qVwh1ptB6zZyu4OxdcI&m=djMG4hE4hqAqXigEOv82HcHoIGCd1_A5RjvzPEXfCUA&s=DzyTOvYGbyl7EPPE-AMGFonC1imhm5IkscXHIKPSuew&e= and include the link in your posting.
>   *****
>
>   Hi;
>                   I'm looking for suggestions and equipment to do thermal imaging through a microscope. We want to image C. elegans to look at their body temperature under different metabolic conditions. While I know they are poikilotherms, we believe they will show a difference to their local background under these conditions. Can you recommend a decent camera for thermal imaging? Are there IR cutoff filters or optics that will not transmit IR wavelengths in a basic fluorescence-type microscope (needed for worm-sized resolution ~10x mag)?
>                   Thanks in advance.
>                   Kathy Spencer
>
>   The Scripps Research Institute
>   Dept of Molecular and Cellular Neuroscience
>   10550 N. Torrey Pines Road
>   DNC 216
>   La Jolla, Ca 92037
>
>
>

[Patrick Van Oostveldt]

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Is imaging necessary? If not i suggest A C.elegans in A microcalorimeter.

Patrick Van Oostveldt
Sint-Denijslaan 199
9000 Gent

Gsm [phone]

Verstuurd vanaf mijn iPhone

> Op 13 okt. 2018 om 06:38 heeft Karel Zuzak <[hidden email]> het volgende geschreven:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Kathy,
>
> I am not aware of any commercially available thermal microscope but that doesn’t mean it doesn’t exist.
>
> While at NIH In the late 90s our lab was exploring various military technologies for medical application and thermal imaging was one.
>
> As you might imagine there have been great advances in thermal imaging as well as NIR based night vision. The advantage of thermal is that it is passive. Meaning one does not need a source. Your subject is the source. Unlike fluorescence or NIR where one must expose the subject to an excitation wavelength or NIR illumination.
>
> So thermal is great but we found a lot of subject to subject variability in temperature which may have been from the ambient surrounding or one of the many physiologic factors influencing temperature.
>
> The other difficulty with thermal was the need for expensive custom optics.
>
> So please know once you get a thermal system working you may encounter a lot of variability from your experiments that will require a lot of control to separate the potentially different sources to metabolism.  
>
> Regarding the optics it’s a question of your budget. Back in the 1990s a system could easily cost over 100k for the camera alone and needed constant liquid nitrogen. The reason being thermal emission occurs around 7 to 14 um. Assuming I’m remembering correctly. A standard ccd or cmos detector won’t detect these wavelengths and standard glass optics won’t transmit those wavelengths sufficiently. So one must use specialized focal plane arrays and optics along with imaging methods using long integration or exposure times or co-adds etc. Then if you need greater magnification and need microscope optics then you need to be sure all the optics in that microscope light path will transmit the thermal wavelengths.  
>
> That said great advances have been made in the thermal field. One can pick up a point/shoot IR thermometer at the hardware store.  As an example  the cost of consumer thermal imaging scopes for hunting applications are down to 5k where previously they could cost up around 80k. I have seen some  as low as 700 but the image resolution and thermal variability reflect that price.  
>
> So if you are set on doing this I would suggest knowing your continuum; the thermal/temperature and imaging sensitivity and resolution. Then I would start with a call to FLIR. They make a variety of thermal and NIR cameras and can possibly help you out. Another place you might like to call are microscope companies such as Zeiss, Nikon, Olympus etc and see what they have to offer.
>
> If after all this you are still interested and have the budget. I’m happy to work with you toward finding/developing a custom solution.  
>
> Warm Regards
> Karel Zuzak
> [hidden email]
> [hidden email]  
>
> 🤠
>
>
> On Oct 12, 2018, at 3:05 PM, Craig Brideau <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> BK7 optical glass, which is pretty common in microscope optics, drops off
> fairly sharply in transmission by 2 to 2.5um, so that will be a problem.
> The last time I heard about a lab attempting thermal imaging they had a
> custom gold-coated reflective objective constructed for the purpose, and
> designed all their other optics with IR glass like zinc selenide or
> similar. It seems unlikely this would work at all through a conventional
> microscope. I can point you to some additional resources if you are
> interested.
>
> Craig
>
> On Fri, Oct 12, 2018 at 12:17 PM Kathryn Spencer <[hidden email]>
> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi;
>>               I'm looking for suggestions and equipment to do thermal
>> imaging through a microscope. We want to image C. elegans to look at their
>> body temperature under different metabolic conditions. While I know they
>> are poikilotherms, we believe they will show a difference to their local
>> background under these conditions. Can you recommend a decent camera for
>> thermal imaging? Are there IR cutoff filters or optics that will not
>> transmit IR wavelengths in a basic fluorescence-type microscope (needed for
>> worm-sized resolution ~10x mag)?
>>               Thanks in advance.
>>               Kathy Spencer
>>
>> The Scripps Research Institute
>> Dept of Molecular and Cellular Neuroscience
>> 10550 N. Torrey Pines Road
>> DNC 216
>> La Jolla, Ca 92037
>>

[kspencer007]

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Thank you everyone for the thoughts and discussion about thermal imaging for C. elegans. I have a lot of reading to do now.
        If this project proceeds, I'll keep you informed of equipment/imaging options that are used.
        Best;
        Kathy Spencer

The Scripps Research Institute
Dept of Molecular and Cellular Neuroscience
10550 N. Torrey Pines Road
DNC 216
La Jolla, Ca 92037

[leavesley]

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Hi Kathy,

One other thing to note, although it's not until February, is that the
Photonics West conference, in the past, has had quite a range of IR
camera vendors as well as custom microscopy and optics companies.  I
believe I even recall seeing some mirror-based objectives on display at
one of the booths.  It could be a good place to scope out the potential
components for a system and discuss options.  I have also seen at least
one infrared microscopy imaging talk presented there (think it was 2
years ago).  Best wishes for a successful microscope build.

Best regards,

Silas

Silas J. Leavesley, Ph.D.
Professor
Department of Chemical and Biomolecular Engineering
Department of Pharmacology
Center for Lung Biology
University of South Alabama
150 Jaguar Drive, SH4129
Mobile, AL 36688
web: http://www.southalabama.edu/centers/bioimaging


On 10/15/2018 4:45 PM, Kathryn Spencer wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Thank you everyone for the thoughts and discussion about thermal imaging for C. elegans. I have a lot of reading to do now.
> If this project proceeds, I'll keep you informed of equipment/imaging options that are used.
> Best;
> Kathy Spencer
>
> The Scripps Research Institute
> Dept of Molecular and Cellular Neuroscience
> 10550 N. Torrey Pines Road
> DNC 216
> La Jolla, Ca 92037
>
>
>
>