timelapse imaging of growing leaves

[Aryeh Weiss]

Is anyone doing long duration (ie, many hours/days) timelapse imaging in
live plants (specifically, leaves). If so, how do you deal with the
movement caused by growth, changes in shape iwth time of day, etc?
The obvious idea is to  images in 3D and also multiple fields to track
it, but we also have problem with photobleaching, so keeping the sample
as static as possible is desirable.

TIA
--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

[Paul Herzmark]

Roger Hangarter at Indiana University has done some great work. Ask him

http://www.bio.indiana.edu/~hangarterlab/





Paul Herzmark
Department of Molecular and Cell Biology
479 Life Science Addition
University of California, Berkeley
Berkeley, CA  94720-3200
[phone]
[phone] fax

On Fri, Oct 16, 2009 at 12:21 AM, Aryeh Weiss <[hidden email]> wrote:

Is anyone doing long duration (ie, many hours/days) timelapse imaging in live plants (specifically, leaves). If so, how do you deal with the movement caused by growth, changes in shape iwth time of day, etc?
The obvious idea is to  images in 3D and also multiple fields to track it, but we also have problem with photobleaching, so keeping the sample as static as possible is desirable.

TIA
--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

[Badri Roysam]

There is also the Computable Project at Caltech is another resource.


Badri Roysam
Professor, Department of Electrical, Computer and Systems Engineering
Associate Director, NSF Center for Subsurface Sensing & Imaging Systems (CenSSIS ERC)
Co-Director, Rensselaer Center for Open Source Software
Rensselaer Polytechnic Institute
110 8th Street, Troy, New York 12180-3590, USA.
Office(JEC 7010): 518-276-8067, Assistant: 518-276-8525, Lab(JEC 6308): 518-276-8207, Fax: [phone]
Email: [hidden email], Web: http://www.ecse.rpi.edu/~roysam



----- Original Message -----
From: Paul Herzmark [mailto:[hidden email]]
To: [hidden email]
Subject: Re: timelapse imaging of growing leaves

> Roger Hangarter at Indiana University has done some great work. Ask him
>
> http://www.bio.indiana.edu/~hangarterlab/
>
>
>
>
>
> Paul Herzmark
> Department of Molecular and Cell Biology
> 479 Life Science Addition
> University of California, Berkeley
> Berkeley, CA  94720-3200
> [phone]
> [phone] fax
>
>
> On Fri, Oct 16, 2009 at 12:21 AM, Aryeh Weiss <[hidden email]> wrote:
>
> > Is anyone doing long duration (ie, many hours/days) timelapse imaging in
> > live plants (specifically, leaves). If so, how do you deal with the
> movement
> > caused by growth, changes in shape iwth time of day, etc?
> > The obvious idea is to  images in 3D and also multiple fields to track it,
> > but we also have problem with photobleaching, so keeping the sample as
> > static as possible is desirable.
> >
> > TIA
> > --aryeh
> > --
> > Aryeh Weiss
> > School of Engineering
> > Bar Ilan University
> > Ramat Gan 52900 Israel
> >
> > Ph:  972-3-5317638
> > FAX: [phone]
> >
>

[Barbara Foster]

Hi, Aryeh

I recommend you contact my colleague, Don Thomson in the UK (see address above).  Don has been doing this sort of thing for decades, with spectacular results.

Good hunting!
Barbara Foster, President and Sr. Consultant

Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: [phone]  Skype: fostermme
W: www.MicroscopyEducation.com

NEWS! Visit the NEW and IMPROVED www.MicroscopyEducation.com! And don't forget:  MME is now scheduling customized, on-site courses for the balance of the year.  Call me for a free assessment and quote.



At 07:23 PM 10/15/2009, you wrote:

Is anyone doing long duration (ie, many hours/days) timelapse imaging in live plants (specifically, leaves). If so, how do you deal with the movement caused by growth, changes in shape iwth time of day, etc?
The obvious idea is to  images in 3D and also multiple fields to track it, but we also have problem with photobleaching, so keeping the sample as static as possible is desirable.

TIA
--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]