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Hello,
I'm hoping to investigate the regenerative time-course of descending neurons in
the zebrafish following laser axotomy. This involves the creation of a trangenic
line of zebrafish which express a fluorescent indicator in a specific cell subset.
Since the laser can only penetrate so far without baking the surrounding tissue,
does anyone have a suggestion as to which cells might be easiest to cut and
visualize? Also, has anyone played around with the wavelength and power
settings needed to selectively disrupt tissue?
Thank you,
DRoumis
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