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Dear
I bring this paper (again) to your attention since today we have better lasers and new tools. More specifically lifetime considerations can help in discriminating against background or reflection and to define an interesting dose for photo activating or exciting under two-photon regime the moles uses of interest.
Best
Alby
https://www.researchgate.net/publication/11829939_Two-photon_microscopy_and_spectroscopy_based_on_a_compact_confocal_scanning_headdo not miss: mix.iit.it
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