update on Yale Microscopy Workshop

[Haberman, Ann]

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Hello fellow microscopy enthusiasts,

We'd like to provide an update on important additions and some changes to the Yale Microscopy Workshop that will be held June 10 - 12th, 2008 in New Haven, CT

Please visit the website for the complete symposium & demonstration schedule, revised equipment list and free online registration http://microscopy.med.yale.edu

1. Many demonstrations on specific techniques or applications will now be offered, including FLIM, laser microdissection, TIRF, structured illumination and live cell imaging.

2. Although the Volocity representative is no longer able to attend, this software will still be available for use. However, the post-acquisition analysis practical that was originally scheduled for Tuesday the 11th at 4:00 is now cancelled. Please note that an Imaris representative will offer their software for rendering and deconvolution all day, every day.

3. The Zeiss LSM 710 confocal will not be equipped with a two-photon laser as originally hoped, but will be operational in every other regard as a spectral confocal. Two other two-photon systems, made by LaVision and Prairie Technologies, remain available for use.

4. Although the classes and hands-on practical sessions are now full, there will be unfettered access to fully functional equipment throughout the day outside of class time periods to anyone who registers.

One hour reservations can be made for many of the instruments after registration. Please view this as an opportunity to perform a "surgical strike" exploration of new instrumentation without any financial or time commitments. Slides and live cell samples will be available for use but feel free to bring your own samples. Individuals interested in bringing their own samples should contact:

organizers
Ann Haberman, Organizer      

[hidden email]

Derek Toomre, Co-organizer     

[hidden email]

Yale Microscopy Workshop and Symposium Schedule

Tuesday June 10th

Morning symposium: Focus on FRET

        Tobias Meyer, Ph.D.
     Department of Chemical and Systems Biology

        Stanford University

        Barbara Baird, M.D. Ph.D.

        Department of Chemistry and Chemical Biology

        Cornell University

Afternoon lecture and hands-on practical (now FULL)    

        "Molecular imaging using FRET microscopy:

        a technical lecture with exemplary research of the immunological synapse"

        Eicke Latz

        Division of Infectious Diseases and Immunology

        University of Massachusetts Medical School

        FRET hands-on practical

        with microscopes from all major vendors

        FRET image analysis practical

        post-acquisition image analysis with Imaris or Volocity

Afternoon Demonstrations:

        TIRF Demonstration (Olympus multi-angle and color TIRF)

        Approaches to Live Cell Imaging (Nikon demo of Biostation IM, A1 confocal &TIRF)

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Wednesday June 11th    

Morning symposium: Focus on 4D time resolved imaging

        Chi-Bin Chien, Ph.D.
    Department of Neurobiology and Anatomy

        University of Utah Medical Center

        Hai Qi, Ph.D.
   Division of Immunology
  NIH NIAID

Afternoon lecture and hands-on practical (now FULL)    

        "Approaches to optical sectioning of tissue:

        confocal or two-photon LSM and other emerging techniques

        Michael Levene
  Department of Biomedical Engineering

        Yale University

        optical sectioning hands-on practical

        thick tissue samples provided as well as fixed cell slides

        3D rendering and deconvolution practical

        post-acquisition image analysis with Imaris or Volocity

Afternoon Demonstrations

        Structured Illumination (Zeiss Apotome demonstration)

        Laser Microdissection (Leica LMD demonstration)

                       

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Thursday June 12th

Morning Demonstration

        Fluorescence Lifetime Imaging (FLIM) (LaVision Biotec)