uv filter

[Boswell, Carl A - (cboswell)]

Dear all,
I have acquired a very cute tiny microscope with white and UV LED illumination http://www.amazon.com/SE-45x-Pocket-Microscope-Light/dp/B002V10862/ref=pd_sxp_grid_pt_0_2 .  I've managed to get a pretty good image of a wasp in amber using my point and shoot camera through this microscope.  The problem is high "UV" reflection resulting in difficulty seeing actual fluorescence.  Does anyone know of a filter, on the order of the flexible wrattan filter just mentioned by Craig that would act as a long pass UV filter?  The main issue is fitting a filter between the light source and the objective.

Thanks in advance for any ideas.
C

Carl A. Boswell
520-742-6131

[Martin Wessendorf-2]

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Dear Carl--

On 2/24/2012 7:26 PM, Boswell, Carl A - (cboswell) wrote:

> I have acquired a very cute tiny microscope with white and UV LED illumination http://www.amazon.com/SE-45x-Pocket-Microscope-Light/dp/B002V10862/ref=pd_sxp_grid_pt_0_2 .  I've managed to get a pretty good image of a wasp in amber using my point and shoot camera through this microscope.  The problem is high "UV" reflection resulting in difficulty seeing actual fluorescence.  Does anyone know of a filter, on the order of the flexible wrattan filter just mentioned by Craig that would act as a long pass UV filter?  The main issue is fitting a filter between the light source and the objective.

A Wratten #2E sounds like it might work:

http://www.tiffen.com/displayproduct.html?tablename=kodakaccessories&itemnum=EK1765114

If that doesn't work, a Schott color filter (KV 418?) might not be that
expensive.

Good luck!

Martin

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Craig Brideau]

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Here's a link to some longpass color glass filters from Edmund.  They're
about $20-60 bucks depending on size:

http://www.edmundoptics.com/products/displayproduct.cfm?productid=1512&PageNum=2&Sort=displayOrder&Order=asc#products

I use similar filters for an epi-fluorescence wide-field scope I threw
together with the LEDs I mentioned WAY back in the LED thread.

Craig



On Sat, Feb 25, 2012 at 8:30 AM, Martin Wessendorf <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> Dear Carl--
>
>
> On 2/24/2012 7:26 PM, Boswell, Carl A - (cboswell) wrote:
>
>  I have acquired a very cute tiny microscope with white and UV LED
>> illumination http://www.amazon.com/SE-45x-**Pocket-Microscope-Light/dp/**
>> B002V10862/ref=pd_sxp_grid_pt_**0_2<http://www.amazon.com/SE-45x-Pocket-Microscope-Light/dp/B002V10862/ref=pd_sxp_grid_pt_0_2>.  I've managed to get a pretty good image of a wasp in amber using my
>> point and shoot camera through this microscope.  The problem is high "UV"
>> reflection resulting in difficulty seeing actual fluorescence.  Does anyone
>> know of a filter, on the order of the flexible wrattan filter just
>> mentioned by Craig that would act as a long pass UV filter?  The main issue
>> is fitting a filter between the light source and the objective.
>>
>
> A Wratten #2E sounds like it might work:
>
> http://www.tiffen.com/**displayproduct.html?tablename=**
> kodakaccessories&itemnum=**EK1765114<http://www.tiffen.com/displayproduct.html?tablename=kodakaccessories&itemnum=EK1765114>
>
> If that doesn't work, a Schott color filter (KV 418?) might not be that
> expensive.
>
> Good luck!
>
> Martin
>
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]
>