why does high NA excitation illumination give better resolution in fluorescence microscopy?

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Daniel White-2 Daniel White-2
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why does high NA excitation illumination give better resolution in fluorescence microscopy?

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Again, thanks all for the illuminating answers!!!

Thanks Andrew and Arne for pointing out that multiplication in spatial
domain is convolution in Fourier domain. With that idea, plus the idea that
only flat featureless objects like the tops of cells have zero axial, z
resolution in widefield, while laterally oriented objects like the sides of
cells, do give resolution in z, which you see as the fat side lobes in z,
axial direction in the widefield OTF,  right?.... it makes sense in the
end.

Could I get away with that description? Assuming I can explain what a
Fourier transform is....? I often use sound waves and different sound pitch
or frequency as an example as people seem to get it expressed that way.

By power I meant power in the Fourier spectrum in that direction. Meaning
you get more than nothing for z resolution.

Cheers, this list rocks!

Dan