Confocal Microscopy List

1x objective

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Jeremy Adler-5

1x objective

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We want to mount a cheap 1x objective on a Zeiss microscope,
Any suggestions

resolution is no very important and we really just want to use the motorized stage to screen a multiwell plate.

====================================
SciLifeLab BioVis Facility
====================================
Jeremy Adler PhD, senior research engineer
Image Analysis and Microscopy
[hidden email]
Tel: +46 70 1679349

Sweden
http://biovis.uu.se
====================================

+46 70 1679349

http://www.biovis.uu.se

weber

Re: 1x objective

Benjamin Smith

Re: 1x objective

In reply to this post by Jeremy Adler-5

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You could use a 150mm focal length achromat, as long as the plate can be that distance from the nose piece.

-Ben Smith

Sent from my iPhone

On Dec 2, 2016, at 3:56 AM, Jeremy Adler <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> We want to mount a cheap 1x objective on a Zeiss microscope,
> Any suggestions
>
> resolution is no very important and we really just want to use the motorized stage to screen a multiwell plate.
>
> ====================================
> SciLifeLab BioVis Facility
> ====================================
> Jeremy Adler PhD, senior research engineer
> Image Analysis and Microscopy
> [hidden email]
> Tel: +46 70 1679349
>
> Sweden
> http://biovis.uu.se
> ====================================
>
>
>
>
> +46 70 1679349
>
> http://www.biovis.uu.se

Julio Vazquez-2

Re: 1x objective

In reply to this post by Jeremy Adler-5

***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** You can find 1x Zeiss microscope objectives on Ebay, and also Olympus objectives which should work too. Depending on the objective and the scope you have, you may need a Zeiss thread adapter.

Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109

On Dec 2, 2016, at 3:56 AM, Jeremy Adler wrote:

We want to mount a cheap 1x objective on a Zeiss microscope,
Any suggestions

George McNamara

Re: 1x objective

In reply to this post by Jeremy Adler-5

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Jeremy,

if your microscope has an optovar built in to the stand, use an empty
position with the optovar in place.

George

On 12/2/2016 5:56 AM, Jeremy Adler wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> We want to mount a cheap 1x objective on a Zeiss microscope,
> Any suggestions
>
> resolution is no very important and we really just want to use the motorized stage to screen a multiwell plate.
>
> ====================================
> SciLifeLab BioVis Facility
> ====================================
> Jeremy Adler PhD, senior research engineer
> Image Analysis and Microscopy
> [hidden email]
> Tel: +46 70 1679349
>
> Sweden
> http://biovis.uu.se
> ====================================
>
>
>
>
> +46 70 1679349
>
> http://www.biovis.uu.se
>

Quintanar, Loren

Re: 1x objective

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

George,

We have an Observer Z1 with a 1x optovar inside. I tried viewing a sample as you suggested and I did not see anything through an empty objective hole...

I suspect that the optovar does not actually form an image? Correct me if I am wrong.

Loren Quintanar
Research Associate II
Light Microscopy Core Facility
Beckman Research Institute
City of Hope
x62874

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara
Sent: Friday, December 02, 2016 9:47 AM
To: [hidden email]
Subject: Re: 1x objective

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Jeremy,

if your microscope has an optovar built in to the stand, use an empty position with the optovar in place.

George

On 12/2/2016 5:56 AM, Jeremy Adler wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> We want to mount a cheap 1x objective on a Zeiss microscope, Any
> suggestions
>
> resolution is no very important and we really just want to use the motorized stage to screen a multiwell plate.
>
> ====================================
> SciLifeLab BioVis Facility
> ====================================
> Jeremy Adler PhD, senior research engineer Image Analysis and
> Microscopy [hidden email]
> Tel: +46 70 1679349
>
> Sweden
> http://biovis.uu.se
> ====================================
>
>
>
>
> +46 70 1679349
>
> http://www.biovis.uu.se
>

--

George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75/
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650

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George McNamara

Re: 1x objective

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Loren,

I do not have access to any high end research microscopes at the moment
- I distinctly remember being able to do this with the Zeiss Axiovert
200's I managed in Miami. However, I now think it was one of these (and
don't have a 'vert at home to test this):

* no objective lens

or

* no objective lens + "telescope" position on the eyepiece turret (which
would only be useful for eye or some kind of eyepiece path -> camera
thingy).

thanks for making me think about this more.

George

On 12/2/2016 3:26 PM, Quintanar, Loren wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> George,
>
> We have an Observer Z1 with a 1x optovar inside. I tried viewing a sample as you suggested and I did not see anything through an empty objective hole...
>
> I suspect that the optovar does not actually form an image? Correct me if I am wrong.
>
> Loren Quintanar
> Research Associate II
> Light Microscopy Core Facility
> Beckman Research Institute
> City of Hope
> x62874
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara
> Sent: Friday, December 02, 2016 9:47 AM
> To: [hidden email]
> Subject: Re: 1x objective
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Jeremy,
>
> if your microscope has an optovar built in to the stand, use an empty position with the optovar in place.
>
> George
>
>
> On 12/2/2016 5:56 AM, Jeremy Adler wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> We want to mount a cheap 1x objective on a Zeiss microscope, Any
>> suggestions
>>
>> resolution is no very important and we really just want to use the motorized stage to screen a multiwell plate.
>>
>> ====================================
>> SciLifeLab BioVis Facility
>> ====================================
>> Jeremy Adler PhD, senior research engineer Image Analysis and
>> Microscopy [hidden email]
>> Tel: +46 70 1679349
>>
>> Sweden
>> http://biovis.uu.se
>> ====================================
>>
>>
>>
>>
>> +46 70 1679349
>>
>> http://www.biovis.uu.se
>>