370nm sources for DNA damage

[Watkins, Simon C]

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Folks, I am modifying a scanner to incorporate a 370-380nm source to generate DNA breaks.  A sort of mega FRAP.  There are many diode solutions out there with just the right wavelength, but which to choose? and whats the best power to select?  if any of you have tried this successfully could you respond, on or off list.
Tx
S

Simon C. Watkins Ph.D, FRC Path
Professor and Vice Chair Cell Biology and Physiology
Professor Immunology Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St
Pittsburgh PA 15261
412-352-2277
www.cbi.pitt.edu<http://www.cbi.pitt.edu>

[Craig Brideau]

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The transmission properties of most optics tends to drop sharply around
400nm.  You will probably lose a lot of power in your lenses.  As a result,
you probably want to get the highest power you can budget for while still
maintaining good noise specs.

Craig


On Wed, Feb 9, 2011 at 8:17 AM, Watkins, Simon C <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Folks, I am modifying a scanner to incorporate a 370-380nm source to
> generate DNA breaks.  A sort of mega FRAP.  There are many diode solutions
> out there with just the right wavelength, but which to choose? and whats the
> best power to select?  if any of you have tried this successfully could you
> respond, on or off list.
> Tx
> S
>
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology and Physiology
> Professor Immunology Director Center for Biologic Imaging
> BSTS 225
> University of Pittsburgh
> 3500 Terrace St
> Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu<http://www.cbi.pitt.edu>
>

[Mike Buchin]

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We have provided LED sources to end users from www.prizmatix.com

They make a 365 LED direct or fiber (3mmm liquid light guide) coupled that
has 50mW plus output (distal; 10nm bw).  The driver has 1 microsecond
off-on-off and can be modulated/pulsed at rates up to 30 kHz.  This is
better than Thorlabs, I believe.  The LED's used are actually fairly
broadband.  I think you get more power as you move up towards 370.

We have used these for exciting Lanthanides and performing TRFM.

If is a macro (well plate or similar sized target), look at
www.innovationsinoptics.com 1240B-100 UV illuminator.  We have one on order
for 365-370 excitation.

Mike

www.stanfordphotonics.com


Michael Buchin
Stanford Photonics, Inc.
Ph: 650-969-5991

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Craig Brideau
Sent: Wednesday, February 09, 2011 9:57 AM
To: [hidden email]
Subject: Re: 370nm sources for DNA damage

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The transmission properties of most optics tends to drop sharply around
400nm.  You will probably lose a lot of power in your lenses.  As a result,
you probably want to get the highest power you can budget for while still
maintaining good noise specs.

Craig


On Wed, Feb 9, 2011 at 8:17 AM, Watkins, Simon C <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Folks, I am modifying a scanner to incorporate a 370-380nm source to
> generate DNA breaks.  A sort of mega FRAP.  There are many diode solutions
> out there with just the right wavelength, but which to choose? and whats
the
> best power to select?  if any of you have tried this successfully could
you

> respond, on or off list.
> Tx
> S
>
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology and Physiology
> Professor Immunology Director Center for Biologic Imaging
> BSTS 225
> University of Pittsburgh
> 3500 Terrace St
> Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu<http://www.cbi.pitt.edu>
>

[Tim O'Brien Sr.]

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If any of your colleagues are interested in applying forces, and
assessing the effects of forces, on cells or other biologic materials,
please let them know about our annual workshop upcoming in May.  Among
the cool features of the workshop are the wide range of techniques used,
the morning lectures, and the hands-on labs in the afternoon.

More information below.

Thanks!

Tim O'Brien
UNC Chapel Hill

Carolina Workshop on Force Measurements and Manipulation in Biological
Microscopy

UNC Chapel Hill; May 10-13, 2011.

Our workshop is hosted by CISMM, our NIH resource (Computer Integrated
Systems for Microscopy and Manipulation; CISMM.org).  It consists of
morning lectures followed by hands-on experiments that use micron-scale
application of forces on live specimens.  The morning discussions
provide a framework for understanding and analyzing forces on the micro
and nanoscales, and serve as an introduction to the afternoon's
experiments. The afternoon sessions are hands-on laboratories where you
perform force measurements such as stretching fibrin fibers and pulling
on living cells. Experiments use laser tweezers, an integrated atomic
force microscopy/optical microscopy system, and 3D magnetic systems
integrated with a fluorescence confocal microscope. We also include an
introduction to microfluidics. Finally, participants spend a half day
learning to use some of the many free software packages available from
our resource that facilitate the analysis and visualization of 3D images
and forces in biological systems. Registration is limited to 18, so you
have lots of hands-on time with the microscopes. The $775 fee includes
all supplies, light breakfasts, snacks and a conference dinner. (A
limited number of scholarships may be available for NIH supported
researchers.)Please contact Cassandra Houston: Ph:[phone]; Email:
[hidden email].







--
E. Tim O'Brien Sr.
Computer Integrated Systems for Microscopy and Manipulation
UNC Physics and Astronomy
UNC Chapel Hill, NC 27599
919 843 2798

[George McNamara]

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Hi Simon,

What about using one of your multiphoton lasers, tune it to ~750 nm, and
use an SHG crystal to convert as much power as possible to ~375 nm. This
would also let you tune up to over 800 nm (peak power) to a wavelength
that is above the absorbance peak of whatever is in there absorbing ~375
nm - at some wavelength (and power) efficiency of generating breaks will
reduce to near zero.

I also believe someone has published a comparison of UV vs 2-photon DNA
breaks.

George

On 2/9/2011 10:17 AM, Watkins, Simon C wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Folks, I am modifying a scanner to incorporate a 370-380nm source to generate DNA breaks.  A sort of mega FRAP.  There are many diode solutions out there with just the right wavelength, but which to choose? and whats the best power to select?  if any of you have tried this successfully could you respond, on or off list.
> Tx
> S
>
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology and Physiology
> Professor Immunology Director Center for Biologic Imaging
> BSTS 225
> University of Pittsburgh
> 3500 Terrace St
> Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu<http://www.cbi.pitt.edu>
>
>    

--


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami

[Steve Bagley]

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Hi Simon

I have recently mounted a Teem Photonics 4mW 355nm laser to the UV port
of our spinning disk system
http://www.photonicsolutions.co.uk/datasheets/teem/TMuchipsealeduv.pdf

This has been suitable for inducing DNA damage in a variety of cell
types. The work has recently been published here:
Molecular Cell,  Volume 41, Issue 1, 7 January 2011, Pages 46-55
http://goo.gl/uuWr6
 
If any details about the equipment and setup will help please do not
hestitate to contact me

Steve

 

Steve Bagley
Head of Imaging
Imaging Facility
Paterson Institute for Cancer Research
Cancer Research UK
University of Manchester
Wilmslow Road
Manchester M20 9BX UK
www.paterson.man.ac.uk


-----Original Message-----
From: Watkins, Simon C [mailto:[hidden email]]
Sent: 09 February 2011 15:18
Subject: 370nm sources for DNA damage

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Folks, I am modifying a scanner to incorporate a 370-380nm source to
generate DNA breaks.  A sort of mega FRAP.  There are many diode
solutions out there with just the right wavelength, but which to choose?
and whats the best power to select?  if any of you have tried this
successfully could you respond, on or off list.
Tx
S

Simon C. Watkins Ph.D, FRC Path
Professor and Vice Chair Cell Biology and Physiology
Professor Immunology Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St
Pittsburgh PA 15261
412-352-2277
www.cbi.pitt.edu<http://www.cbi.pitt.edu>

[Watkins, Simon C]

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To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi steve.   What did the source end up costing you?

Simon C Watkins
Professor and Vice Chair. Cell Biology
Professor Immunology
Director Center for Biologic Imaging
University of Pittsburgh
BSTS 225, Terrace St.
Pittsburgh PA 15261
Tel: [phone]
Http://www.cbi.pitt.edu

And sent from iPad hence the typos

On Feb 14, 2011, at 4:30 AM, "Steve Bagley" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Simon
>
> I have recently mounted a Teem Photonics 4mW 355nm laser to the UV port
> of our spinning disk system
> http://www.photonicsolutions.co.uk/datasheets/teem/TMuchipsealeduv.pdf
>
> This has been suitable for inducing DNA damage in a variety of cell
> types. The work has recently been published here:
> Molecular Cell,  Volume 41, Issue 1, 7 January 2011, Pages 46-55
> http://goo.gl/uuWr6
>
> If any details about the equipment and setup will help please do not
> hestitate to contact me
>
> Steve
>
>
>
> Steve Bagley
> Head of Imaging
> Imaging Facility
> Paterson Institute for Cancer Research
> Cancer Research UK
> University of Manchester
> Wilmslow Road
> Manchester M20 9BX UK
> www.paterson.man.ac.uk
>
>
> -----Original Message-----
> From: Watkins, Simon C [mailto:[hidden email]]
> Sent: 09 February 2011 15:18
> Subject: 370nm sources for DNA damage
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Folks, I am modifying a scanner to incorporate a 370-380nm source to
> generate DNA breaks.  A sort of mega FRAP.  There are many diode
> solutions out there with just the right wavelength, but which to choose?
> and whats the best power to select?  if any of you have tried this
> successfully could you respond, on or off list.
> Tx
> S
>
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology and Physiology
> Professor Immunology Director Center for Biologic Imaging
> BSTS 225
> University of Pittsburgh
> 3500 Terrace St
> Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu<http://www.cbi.pitt.edu>