Manuel Pele |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi everyone, I'm looking for a way to reconstruct a full 3D image of my samples that I need to image using serial sections. Any tips about which software I can use ? Does it work well? Cheers Manuel -- Manuel PELE, PhD Australian Regenerative Medicine Institute, Building 75, Level 1 Monash University, Clayton Campus Wellington Road Clayton, VIC 3800 AUSTRALIA Ph: +61 3 9902 9648 Email: [hidden email] |
Martin Wessendorf-2 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Manuel-- ImageJ (or better yet, its Fiji implementation) is probably the place to start, since it's free and there's a user listserv even more active than this one. ImageJ (including its listserv) is found at: http://rsbweb.nih.gov/ij/ Fiji is at: http://fiji.sc/wiki/index.php/Fiji Good luck! Martin Wessendorf On 5/13/2012 10:13 PM, Manuel Pele wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi everyone, > > I'm looking for a way to reconstruct a full 3D image of my samples that I > need to image using serial sections. Any tips about which software I can > use ? Does it work well? > > Cheers > > Manuel > -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 e-mail: [hidden email] |
Cameron Nowell |
In reply to this post by Manuel Pele
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Manuel, We use the registration plugins that can be found in Fiji (www.fiji.sc). StackReg works quite well, you can find more info on it here http://bigwww.epfl.ch/thevenaz/stackreg/ Cheers Cam Cameron J. Nowell Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research Melbourne - Parkville Branch PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA Office: +61 3 9341 3158 Mobile: +61 422882700 Fax: +61 3 9341 3104 Facility Website Linked In Profile -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Manuel Pele Sent: Monday, 14 May 2012 1:14 PM To: [hidden email] Subject: 3D reconstruction from serial sections ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi everyone, I'm looking for a way to reconstruct a full 3D image of my samples that I need to image using serial sections. Any tips about which software I can use ? Does it work well? Cheers Manuel -- Manuel PELE, PhD Australian Regenerative Medicine Institute, Building 75, Level 1 Monash University, Clayton Campus Wellington Road Clayton, VIC 3800 AUSTRALIA Ph: +61 3 9902 9648 Email: [hidden email] |
Eric Scarfone |
In reply to this post by Martin Wessendorf-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi there, In the past I've used the Autoaligner module of Imaris that worked remarkably well! Eric Ps. No commercial interest! **************PLEASE NOTE NEW ADRESS ************ Eric Scarfone, PhD Research Scientist, CNRS & Department of Neuroscience, Karolinska Institutet Mob: +46 (0)70 888 2352 Visiting address: Science for Life Laboratory, Advanced Light Microscopy Floor 1, room 1410 Tomtebodavägen 23A, Solna, Sweden Postal address: SciLifeLab PO Box 1031 17121 Solna Sweden -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf Sent: 15 May 2012 03:12 To: [hidden email] Subject: Re: 3D reconstruction from serial sections ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Manuel-- ImageJ (or better yet, its Fiji implementation) is probably the place to start, since it's free and there's a user listserv even more active than this one. ImageJ (including its listserv) is found at: http://rsbweb.nih.gov/ij/ Fiji is at: http://fiji.sc/wiki/index.php/Fiji Good luck! Martin Wessendorf On 5/13/2012 10:13 PM, Manuel Pele wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi everyone, > > I'm looking for a way to reconstruct a full 3D image of my samples > that I need to image using serial sections. Any tips about which > software I can use ? Does it work well? > > Cheers > > Manuel > -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 e-mail: [hidden email] |
Anton Kamnev |
In reply to this post by Manuel Pele
Dear Manuel, In our lab we are using Huygens software from SVI for 3D reconstruction of structures from Z-stacks. It is a bit expensive, but works very well and have a lot of useful tools for structure analysis. Moreover, it does deconvolution as well.
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Michael Doube |
In reply to this post by Manuel Pele
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Manuel, > I'm looking for a way to reconstruct a full 3D image of my samples that I > need to image using serial sections. Any tips about which software I can > use ? Does it work well? Once you've done the registration to align your serial sections, you could try Drishti for 3D visualisation: http://anusf.anu.edu.au/Vizlab/drishti/ Michael |
Peter Gabriel Pitrone |
In reply to this post by Manuel Pele
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Howdy Manuel, This tool is already incorporated into TrakEM2 (http://fiji.sc/wiki/index.php/TrakEM2) using an elastic alignment (http://fiji.sc/wiki/index.php/Elastic_Alignment_and_Montage) Here are some practical examples: http://fly.mpi-cbg.de/~saalfeld/video/elastic2.mov http://fly.mpi-cbg.de/~saalfeld/video/elastic2-reslice.mov I hope that this helps, Pete Peter Gabriel Pitrone TechRMS Microscopy & Imaging Specialist in Professor Pavel Tomancak's group at Max Planck Institute for Molecular Cell Biology and Genetics, Dresden Pfotenhauerstrasse 108 01307, Dresden Germany +49 (0) 351 210 2687 [hidden email] https://wiki.mpi-cbg.de/wiki/openspim On May 14, 2012, at 5:13 AM, Manuel Pele wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi everyone, > > I'm looking for a way to reconstruct a full 3D image of my samples that I > need to image using serial sections. Any tips about which software I can > use ? Does it work well? > > Cheers > > Manuel > > -- > Manuel PELE, PhD > > Australian Regenerative Medicine Institute, > Building 75, Level 1 > Monash University, Clayton Campus > Wellington Road > Clayton, VIC 3800 > AUSTRALIA > > Ph: +61 3 9902 9648 > Email: [hidden email] |
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