3D reconstruction from serial sections

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Manuel Pele Manuel Pele
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3D reconstruction from serial sections

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Hi everyone,

I'm looking for a way to reconstruct a full 3D image of my samples that I
need to image using serial sections. Any tips about which software I can
use ? Does it work well?

Cheers

Manuel

--
Manuel PELE, PhD

Australian Regenerative Medicine Institute,
Building 75, Level 1
Monash University, Clayton Campus
Wellington Road
Clayton, VIC 3800
AUSTRALIA

Ph: +61 3 9902 9648
Email: [hidden email]
Martin Wessendorf-2 Martin Wessendorf-2
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Re: 3D reconstruction from serial sections

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Dear Manuel--

ImageJ (or better yet, its Fiji implementation) is probably the place to
start, since it's free and there's a user listserv even more active than
this one.

ImageJ (including its listserv) is found at: http://rsbweb.nih.gov/ij/

Fiji is at: http://fiji.sc/wiki/index.php/Fiji

Good luck!

Martin Wessendorf

On 5/13/2012 10:13 PM, Manuel Pele wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi everyone,
>
> I'm looking for a way to reconstruct a full 3D image of my samples that I
> need to image using serial sections. Any tips about which software I can
> use ? Does it work well?
>
> Cheers
>
> Manuel
>



--
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455                    e-mail: [hidden email]
Cameron Nowell Cameron Nowell
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Re: 3D reconstruction from serial sections

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Hi Manuel,

We use the registration plugins that can be found in Fiji (www.fiji.sc).

StackReg works quite well, you can find more info on it here

http://bigwww.epfl.ch/thevenaz/stackreg/


Cheers

Cam



Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research Melbourne - Parkville Branch
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
Office: +61 3 9341 3158
Mobile: +61 422882700
Fax: +61 3 9341 3104
Facility Website
Linked In Profile





-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Manuel Pele
Sent: Monday, 14 May 2012 1:14 PM
To: [hidden email]
Subject: 3D reconstruction from serial sections

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi everyone,

I'm looking for a way to reconstruct a full 3D image of my samples that
I need to image using serial sections. Any tips about which software I
can use ? Does it work well?

Cheers

Manuel

--
Manuel PELE, PhD

Australian Regenerative Medicine Institute, Building 75, Level 1 Monash
University, Clayton Campus Wellington Road Clayton, VIC 3800 AUSTRALIA

Ph: +61 3 9902 9648
Email: [hidden email]
Eric Scarfone Eric Scarfone
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Re: 3D reconstruction from serial sections

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Hi there,
In the past I've used the Autoaligner module of Imaris that worked remarkably well!
Eric
 
Ps. No commercial interest!


**************PLEASE NOTE NEW ADRESS ************
Eric Scarfone, PhD
Research Scientist,
CNRS & Department of Neuroscience, Karolinska Institutet

 Mob: +46 (0)70 888 2352

 Visiting address:
Science for Life Laboratory,
Advanced Light Microscopy
Floor 1, room 1410
Tomtebodavägen 23A, Solna, Sweden

 Postal address:
 SciLifeLab
 PO Box 1031
 17121 Solna
 Sweden


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
Sent: 15 May 2012 03:12
To: [hidden email]
Subject: Re: 3D reconstruction from serial sections

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear Manuel--

ImageJ (or better yet, its Fiji implementation) is probably the place to start, since it's free and there's a user listserv even more active than this one.

ImageJ (including its listserv) is found at: http://rsbweb.nih.gov/ij/

Fiji is at: http://fiji.sc/wiki/index.php/Fiji

Good luck!

Martin Wessendorf

On 5/13/2012 10:13 PM, Manuel Pele wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi everyone,
>
> I'm looking for a way to reconstruct a full 3D image of my samples
> that I need to image using serial sections. Any tips about which
> software I can use ? Does it work well?
>
> Cheers
>
> Manuel
>



--
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455                    e-mail: [hidden email]
Anton Kamnev Anton Kamnev
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Re: 3D reconstruction from serial sections

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Dear Manuel,

In our lab we are using Huygens software  from SVI for 3D reconstruction of structures from Z-stacks. It is a bit expensive, but works very well and have a lot of useful tools for structure analysis. Moreover, it does deconvolution as well.

Manuel Pele wrote
*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

Hi everyone,

I'm looking for a way to reconstruct a full 3D image of my samples that I
need to image using serial sections. Any tips about which software I can
use ? Does it work well?

Cheers

Manuel

--
Manuel PELE, PhD

Australian Regenerative Medicine Institute,
Building 75, Level 1
Monash University, Clayton Campus
Wellington Road
Clayton, VIC 3800
AUSTRALIA

Ph: +61 3 9902 9648
Email: [hidden email]
Michael Doube Michael Doube
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Re: 3D reconstruction from serial sections

In reply to this post by Manuel Pele
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To join, leave or search the confocal microscopy listserv, go to:
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Hi Manuel,

> I'm looking for a way to reconstruct a full 3D image of my samples that I
> need to image using serial sections. Any tips about which software I can
> use ? Does it work well?

Once you've done the registration to align your serial sections, you
could try Drishti for 3D visualisation:

http://anusf.anu.edu.au/Vizlab/drishti/

Michael
Peter Gabriel Pitrone Peter Gabriel Pitrone
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Re: 3D reconstruction from serial sections

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Howdy Manuel,

This tool is already incorporated into TrakEM2 (http://fiji.sc/wiki/index.php/TrakEM2) using an elastic alignment (http://fiji.sc/wiki/index.php/Elastic_Alignment_and_Montage) Here are some practical examples:

http://fly.mpi-cbg.de/~saalfeld/video/elastic2.mov

http://fly.mpi-cbg.de/~saalfeld/video/elastic2-reslice.mov

I hope that this helps,

Pete

Peter Gabriel Pitrone TechRMS
Microscopy & Imaging Specialist
in Professor Pavel Tomancak's group
at Max Planck Institute for Molecular
Cell Biology and Genetics, Dresden
Pfotenhauerstrasse 108
01307, Dresden
Germany

+49 (0) 351 210 2687
[hidden email]
https://wiki.mpi-cbg.de/wiki/openspim




On May 14, 2012, at 5:13 AM, Manuel Pele wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi everyone,
>
> I'm looking for a way to reconstruct a full 3D image of my samples that I
> need to image using serial sections. Any tips about which software I can
> use ? Does it work well?
>
> Cheers
>
> Manuel
>
> --
> Manuel PELE, PhD
>
> Australian Regenerative Medicine Institute,
> Building 75, Level 1
> Monash University, Clayton Campus
> Wellington Road
> Clayton, VIC 3800
> AUSTRALIA
>
> Ph: +61 3 9902 9648
> Email: [hidden email]