Advice on high-end light-sheet

[F Javier Diez Guerra]

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Hi,

We are considering several options for a high-end light-sheet (lattice)
system and would like to know your experience. The system is meant for
our advanced light microscopy facility at the CBMSO in Madrid, with many
users and projects from different fields of the biosciences. So we value
versatility and robustness, in addition to specifications.

I would be very grateful to listen to your comments off-list (if you
think it should be that way).

Thanks all for your cooperation.

Best

Javier


F Javier Diez-Guerra, PhD
Servicio de Microscopía Confocal
Centro de Biologia Molecular Severo Ochoa
C/ Nicolás Cabrera, 1
Campus de Cantoblanco
28049 Madrid
SPAIN

Tel     [phone]
e-mail: [hidden email]

[Zdenek Svindrych-2]

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Versatility?
A good microscope should be able to deal with any sample you throw at it,
right?
Not so with a lightsheet microscope. You need to decide whether you need
large sample volumes (Lavision Ultramicroscope, Zeiss Lightsheet Z.1 - now
apparently rebranded as " Lightsheet 7") or high resolution (3i lattice
lightsheet or Zeiss "Lattice Lightsheet 7" - how confusing).
If you want both options, you're going to need two separate instruments...
Absolutely zero commercial interest.
Best, zdenek

On Wed, Jun 2, 2021 at 3:15 PM F Javier Díez Guerra <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> We are considering several options for a high-end light-sheet (lattice)
> system and would like to know your experience. The system is meant for
> our advanced light microscopy facility at the CBMSO in Madrid, with many
> users and projects from different fields of the biosciences. So we value
> versatility and robustness, in addition to specifications.
>
> I would be very grateful to listen to your comments off-list (if you
> think it should be that way).
>
> Thanks all for your cooperation.
>
> Best
>
> Javier
>
>
> F Javier Diez-Guerra, PhD
> Servicio de Microscopía Confocal
> Centro de Biologia Molecular Severo Ochoa
> C/ Nicolás Cabrera, 1
> Campus de Cantoblanco
> 28049 Madrid
> SPAIN
>
> Tel     [phone]
> e-mail: [hidden email]
>

--
--
Zdenek Svindrych, Ph.D.
Research Scientist - Microscopy Imaging Specialist
Department of Biochemistry and Cell Biology
Geisel School of Medicine at Dartmouth

[Armstrong, Brian]

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Hi, well, a great answer from Zdenek.
The Zeiss Lightsheet 7 can do both large samples (think cleared mouse brain) and small samples (think small transparent organoid), but you need two completely different stage set-ups.
Bruker simply advises you to purchase two separate systems. Maybe a good idea.

Versatility is not the strong point of a lattice light sheet.
Would I like to have one? Sure, however I think it would be difficult to justify based on the number of samples that could be imaged.  

Cheers,

Brian Armstrong PhD
Associate Research Professor
Director, Light Microscopy Core Facility
Beckman Research Institute, City of Hope

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Zdenek Svindrych
Sent: Wednesday, June 02, 2021 12:54 PM
To: [hidden email]
Subject: Re: Advice on high-end light-sheet

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Versatility?
A good microscope should be able to deal with any sample you throw at it, right?
Not so with a lightsheet microscope. You need to decide whether you need large sample volumes (Lavision Ultramicroscope, Zeiss Lightsheet Z.1 - now apparently rebranded as " Lightsheet 7") or high resolution (3i lattice lightsheet or Zeiss "Lattice Lightsheet 7" - how confusing).
If you want both options, you're going to need two separate instruments...
Absolutely zero commercial interest.
Best, zdenek

On Wed, Jun 2, 2021 at 3:15 PM F Javier Díez Guerra <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.com/v3/__http://lists.umn.edu/cgi-bin/wa?A0=confoca
> lmicroscopy__;!!Fou38LsQmgU!4IMIBO6Fq-O_-rhHGN8f4UnBvNe2ExZFBtjLk86QUN
> -a-S0P2W7Ku9b3KJ500lk$ Post images on
> https://urldefense.com/v3/__http://www.imgur.com__;!!Fou38LsQmgU!4IMIBO6Fq-O_-rhHGN8f4UnBvNe2ExZFBtjLk86QUN-a-S0P2W7Ku9b3abvHTs4$  and include the link in your posting.
> *****
>
> Hi,
>
> We are considering several options for a high-end light-sheet
> (lattice) system and would like to know your experience. The system is
> meant for our advanced light microscopy facility at the CBMSO in
> Madrid, with many users and projects from different fields of the
> biosciences. So we value versatility and robustness, in addition to specifications.
>
> I would be very grateful to listen to your comments off-list (if you
> think it should be that way).
>
> Thanks all for your cooperation.
>
> Best
>
> Javier
>
>
> F Javier Diez-Guerra, PhD
> Servicio de Microscopía Confocal
> Centro de Biologia Molecular Severo Ochoa C/ Nicolás Cabrera, 1 Campus
> de Cantoblanco
> 28049 Madrid
> SPAIN
>
> Tel     [phone]
> e-mail: [hidden email]
>

--
--
Zdenek Svindrych, Ph.D.
Research Scientist - Microscopy Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth

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[F Javier Diez Guerra]

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Thanks Zdenek,

Very clear and sound!

For a better understanding from my side and context, please translate
"versatility" as "accommodation to as many different applications and
user requests as possible". And yes, you are right on the size of the
sample.

As I understand it, most of our users would be more on the gaussian side
of light sheet (bigger samples, embryos, etc), and I was wondering what
would happen with those interested in imaging medium-sized samples, such
as organoids, etc. Lattice light sheet will surely be very kind to
cultured cells and much more resolutive. But for now, we have other
instruments (spinning disk, STED) that cover a wide range of
applications in this category.

As in my earlier email, I'll be very interested and appreciate any
experienced input.

Best

Javier



El 02/06/2021 a las 21:54, Zdenek Svindrych escribió:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Versatility?
> A good microscope should be able to deal with any sample you throw at it,
> right?
> Not so with a lightsheet microscope. You need to decide whether you need
> large sample volumes (Lavision Ultramicroscope, Zeiss Lightsheet Z.1 - now
> apparently rebranded as " Lightsheet 7") or high resolution (3i lattice
> lightsheet or Zeiss "Lattice Lightsheet 7" - how confusing).
> If you want both options, you're going to need two separate instruments...
> Absolutely zero commercial interest.
> Best, zdenek
>
> On Wed, Jun 2, 2021 at 3:15 PM F Javier Díez Guerra <[hidden email]>
> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi,
>>
>> We are considering several options for a high-end light-sheet (lattice)
>> system and would like to know your experience. The system is meant for
>> our advanced light microscopy facility at the CBMSO in Madrid, with many
>> users and projects from different fields of the biosciences. So we value
>> versatility and robustness, in addition to specifications.
>>
>> I would be very grateful to listen to your comments off-list (if you
>> think it should be that way).
>>
>> Thanks all for your cooperation.
>>
>> Best
>>
>> Javier
>>
>>
>> F Javier Diez-Guerra, PhD
>> Servicio de Microscopía Confocal
>> Centro de Biologia Molecular Severo Ochoa
>> C/ Nicolás Cabrera, 1
>> Campus de Cantoblanco
>> 28049 Madrid
>> SPAIN
>>
>> Tel     [phone]
>> e-mail: [hidden email]
>>
>

[Sylvie Le Guyader]

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*****

Hi Javier

We have an Aurora light sheet from MSquared Lasers and are delighted with it. It is very versatile and with its Airy beam, offers high res across a large (around 800 um) FOV. :)

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut
Hälsovägen 7C,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website
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-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of F Javier Díez Guerra
Sent: 02 June 2021 20:15
To: [hidden email]
Subject: Advice on high-end light-sheet

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Hi,

We are considering several options for a high-end light-sheet (lattice) system and would like to know your experience. The system is meant for our advanced light microscopy facility at the CBMSO in Madrid, with many users and projects from different fields of the biosciences. So we value versatility and robustness, in addition to specifications.

I would be very grateful to listen to your comments off-list (if you think it should be that way).

Thanks all for your cooperation.

Best

Javier


F Javier Diez-Guerra, PhD
Servicio de Microscopía Confocal
Centro de Biologia Molecular Severo Ochoa C/ Nicolás Cabrera, 1 Campus de Cantoblanco
28049 Madrid
SPAIN

Tel     [phone]
e-mail: [hidden email]


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