Android Tool for Microscopy
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Andrew Barlow |
Android Tool for Microscopy
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Sylvie Le Guyader |
Re: Android Tool for Microscopy
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Andrew Barlow |
Re: Android Tool for Microscopy
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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Thanks Sylvie!
That's much appreciated! Please let me know if you have any suggestions for improvement or new functionality.
Regards, Andy
From: Confocal Microscopy List <[hidden email]> on behalf of Sylvie Le Guyader <[hidden email]>
Sent: 12 December 2016 09:27 To: [hidden email] Subject: Re: Android Tool for Microscopy ***** To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on
http://www.imgur.com and include the link in your posting. *****
Cool app! Thanks Andrew! :-)
Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Hälsovägen 7, Novum, G lift, floor 6 <a href="tel:14157">14157 Huddinge Sweden mobile: <a href="tel:+46 (0) 73 733 5008">+46 (0) 73 733 5008 office: <a href="tel:+46 (0) 08-524 811 72">+46 (0) 08-524 811 72 LCI website ---- Andrew Barlow wrote ---- *****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear Confocal Microscopy List, I'd like to draw your attention to a new tool for microscopists I've developed for Android devices, "Resolution". After entering magnification, immersion medium, lambda, and NA the App will calculate resolution (actual and theoretical), axial resolution and fluorescence brightness of your objective. Each objective entered can be easily saved and restored. You can select your camera, binning and additional magnification to determine if you're sampling at Nyquist frequency. All information generated get be easily shared via e-mail, MMS, LinkedIn or even Facebook (it could with the share button. The App is free to download and compatible with phones or tablets running Android 4.0 or above. I hope you find it useful! Andrew L. Barlow Please use the link below on your Android device to install: https://play.google.com/store/apps/details?id=com.Barlowax.resolutionfragments You can also find it on the Play Store by searching for the Keywords "Resolution Objective" |
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Göran Månsson |
Re: Android Tool for Microscopy
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Tim Feinstein |
Re: Android Tool for Microscopy
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Andrew Barlow |
Re: Android Tool for Microscopy
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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Hi Tim,
I plan to this.
It's taken me six months to go from no android experience to publishing, working in my spare time.
I think it'll be similar for iOS but I'd really like to do it.
I also plan to maintain and expand the capabilities of the Android App.
Regards, Andy
From: Confocal Microscopy List <[hidden email]> on behalf of Feinstein, Timothy N <[hidden email]>
Sent: 12 December 2016 13:58 To: [hidden email] Subject: Re: Android Tool for Microscopy *****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
***** I understand it takes twice the work to make the same thing for iOS, but it would be great if you did. The same request goes out to SVI. Please? T Timothy Feinstein, Ph.D. Research Scientist University of Pittsburgh Department of Developmental Biology On 12/12/16, 6:41 AM, "Confocal Microscopy List on behalf of Göran Månsson" <[hidden email] on behalf of [hidden email]> wrote: >***** >To join, leave or search the confocal microscopy listserv, go to: >https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn. >edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=01%7C01%7Ctnf8%40PITT.ED >U%7C0695e1d68a454654d5a508d42283f5c1%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C >1&sdata=WnE1qdQb0UbPqgITDORByvfsrByTysGqdHeR6tEpMjY%3D&reserved=0 >Post images on >https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur. >com&data=01%7C01%7Ctnf8%40PITT.EDU%7C0695e1d68a454654d5a508d42283f5c1%7C9e >f9f489e0a04eeb87cc3a526112fd0d%7C1&sdata=YSV%2FxsJj2iyLW3VVRg9bnJKrIGwGgur >JHDsv8ZgA9Sk%3D&reserved=0 and include the link in your posting. >***** > >Hi Andrew, > >I just downloaded it, looks great! Thanks for sharing it with the >community. > >Best >Göran > > > >-----Original Message----- >From: Confocal Microscopy List [[hidden email]] >On >Behalf Of Andrew Barlow >Sent: den 11 december 2016 23:12 >To: [hidden email] >Subject: Android Tool for Microscopy > >***** >To join, leave or search the confocal microscopy listserv, go to: >https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn. >edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=01%7C01%7Ctnf8%40PITT.ED >U%7C0695e1d68a454654d5a508d42283f5c1%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C >1&sdata=WnE1qdQb0UbPqgITDORByvfsrByTysGqdHeR6tEpMjY%3D&reserved=0 >Post images on >https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur. >com&data=01%7C01%7Ctnf8%40PITT.EDU%7C0695e1d68a454654d5a508d42283f5c1%7C9e >f9f489e0a04eeb87cc3a526112fd0d%7C1&sdata=YSV%2FxsJj2iyLW3VVRg9bnJKrIGwGgur >JHDsv8ZgA9Sk%3D&reserved=0 and include the link in your posting. >***** > >Dear Confocal Microscopy List, > >I'd like to draw your attention to a new tool for microscopists I've >developed for Android devices, "Resolution". > >After entering magnification, immersion medium, lambda, and NA the App >will >calculate resolution (actual and theoretical), axial resolution and >fluorescence brightness of your objective. > >Each objective entered can be easily saved and restored. > >You can select your camera, binning and additional magnification to >determine if you're sampling at Nyquist frequency. > >All information generated get be easily shared via e-mail, MMS, LinkedIn >or >even Facebook (it could with the share button. > >The App is free to download and compatible with phones or tablets running >Android 4.0 or above. > >I hope you find it useful! > >Andrew L. Barlow > > > >Please use the link below on your Android device to install: > >https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fplay.goog >le.com%2Fstore%2Fapps%2Fdetails%3Fid%3Dcom.Barlowax.resolutionfragmen&data >=01%7C01%7Ctnf8%40PITT.EDU%7C0695e1d68a454654d5a508d42283f5c1%7C9ef9f489e0 >a04eeb87cc3a526112fd0d%7C1&sdata=ciPXvJXUHfvl54rpQifbkjsLUnueXTzC7YynoWXFK >RY%3D&reserved=0 >ts > >You can also find it on the Play Store by searching for the Keywords >"Resolution Objective" > > > > > |
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Louise Armstrong |
Re: Android Tool for Microscopy
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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
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I think it's great Andy, and can't wait for the iOS version. Well done and thank you! Louise Sent from my iPhone
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Alex Asanov |
Re: Android Tool for Microscopy
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In reply to this post by Andrew Barlow
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Hi Andy,
Your tool will be useful for many microscopists,
including TIRF users. We need it for iPhones too. Unfortunately, I do not have
Android and was not able to see which equation did you use for fluorescence
brightness. How you define the fluorescence
brightness? Did you use for fluorescence brightness ~(NA)^4
(X)^-2? This equation is good for epi-fluorescence and objective-TIRF, when
both excitation and emission use the objective. In the case of prism- and
lightguide-TIRF, where the excitation lightpath is independent from the emission
channel, however, the amount of emitted fluorescence collected
by an objective to a pixel of CCD camera is proportional to the square of
numerical aperture and reverse proportional to magnification ~(NA)^2 (X)^-1. If
you distinguish between these TIRF geometries, you will benefit the entire
microscopists community. Prism- and lightguide-TIRF are
becoming indispensable tools for super-resolution microscopies and single
molecule studies, becasue they provide "clean" TIRF effect, unlike o-TIRF, which
is contaminated with 15-20% of stray light.
It is important to distingushe between p-,
lg-, and o-TIRF geometries.
Your app is very much needed tool! Thank
you.
Best
regards, Alexander Asanov,
Ph.D. From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Andrew Barlow Sent: Monday, December 12, 2016 4:45 AM To: [hidden email] Subject: Re: Android Tool for Microscopy Thanks Sylvie! That's much appreciated! Please let me know if you have any suggestions for improvement or new functionality. Regards, Andy From: Confocal Microscopy List
<[hidden email]> on behalf of Sylvie Le Guyader
<[hidden email]>
Sent: 12 December 2016 09:27 To: [hidden email] Subject: Re: Android Tool for Microscopy ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your
posting. *****
Cool app! Thanks Andrew! :-)
Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Hälsovägen 7, Novum, G lift, floor 6 <A href="tel:14157">14157 Huddinge Sweden mobile: <A href="tel:+46 (0) 73 733 5008">+46 (0) 73 733 5008 office: <A href="tel:+46 (0) 08-524 811 72">+46 (0) 08-524 811 72 LCI website ---- Andrew Barlow wrote ---- ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear Confocal Microscopy List, I'd like to draw your attention to a new tool for microscopists I've developed for Android devices, "Resolution". After entering magnification, immersion medium, lambda, and NA the App will calculate resolution (actual and theoretical), axial resolution and fluorescence brightness of your objective. Each objective entered can be easily saved and restored. You can select your camera, binning and additional magnification to determine if you're sampling at Nyquist frequency. All information generated get be easily shared via e-mail, MMS, LinkedIn or even Facebook (it could with the share button. The App is free to download and compatible with phones or tablets running Android 4.0 or above. I hope you find it useful! Andrew L. Barlow Please use the link below on your Android device to install: https://play.google.com/store/apps/details?id=com.Barlowax.resolutionfragments You can also find it on the Play Store by searching for the Keywords "Resolution Objective" |
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Andrew Barlow |
Re: Android Tool for Microscopy
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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Hi Alex,
Thanks for the message.
Yes, I used (NA)^4 (X)^-2 for brightness, this value is divided by the brightness of a 10x 0.25 objective, which I'm using as "standard brightness" to give the value scale.
Your suggestions for TIRF are really interesting, and I'd like to discuss these in more detail "off list".
Thanks, Andy
From: Confocal Microscopy List <[hidden email]> on behalf of Alex Asanov <[hidden email]>
Sent: 12 December 2016 18:24 To: [hidden email] Subject: Re: Android Tool for Microscopy ***** To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on
http://www.imgur.com and include the link in your posting. *****
Hi Andy,
Your tool will be useful for many microscopists, including TIRF users. We need it for iPhones too. Unfortunately, I do not have Android
and was not able to see which equation did you use for fluorescence brightness. How you define the fluorescence brightness? Did you use for fluorescence
brightness ~(NA)^4 (X)^-2? This equation is good for epi-fluorescence and objective-TIRF, when both excitation and emission use the objective. In the case of prism- and lightguide-TIRF, where the excitation lightpath is independent from the emission channel,
however, the amount of emitted fluorescence collected by an objective to a pixel of CCD camera is proportional to the square of numerical aperture and reverse proportional to magnification ~(NA)^2 (X)^-1. If you distinguish between these TIRF geometries,
you will benefit the entire microscopists community. Prism- and lightguide-TIRF are becoming indispensable tools for super-resolution microscopies and single molecule studies, becasue they provide "clean" TIRF effect, unlike o-TIRF, which is contaminated with
15-20% of stray light.
It is important to distingushe between p-, lg-, and o-TIRF geometries.
Your app is very much needed tool! Thank you.
Best regards, Alexander Asanov, Ph.D. From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Andrew Barlow Sent: Monday, December 12, 2016 4:45 AM To: [hidden email] Subject: Re: Android Tool for Microscopy Thanks Sylvie!
That's much appreciated! Please let me know if you have any suggestions for improvement or new functionality.
Regards, Andy
From: Confocal Microscopy List <[hidden email]> on behalf of Sylvie Le Guyader <[hidden email]>
Sent: 12 December 2016 09:27 To: [hidden email] Subject: Re: Android Tool for Microscopy ***** To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on
http://www.imgur.com and include the link in your posting. *****
Cool app! Thanks Andrew! :-)
Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Hälsovägen 7, Novum, G lift, floor 6 <a href="tel:14157">14157 Huddinge Sweden mobile: <a href="tel:+46 (0) 73 733 5008">+46 (0) 73 733 5008 office: <a href="tel:+46 (0) 08-524 811 72">+46 (0) 08-524 811 72 LCI website ---- Andrew Barlow wrote ---- *****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear Confocal Microscopy List, I'd like to draw your attention to a new tool for microscopists I've developed for Android devices, "Resolution". After entering magnification, immersion medium, lambda, and NA the App will calculate resolution (actual and theoretical), axial resolution and fluorescence brightness of your objective. Each objective entered can be easily saved and restored. You can select your camera, binning and additional magnification to determine if you're sampling at Nyquist frequency. All information generated get be easily shared via e-mail, MMS, LinkedIn or even Facebook (it could with the share button. The App is free to download and compatible with phones or tablets running Android 4.0 or above. I hope you find it useful! Andrew L. Barlow Please use the link below on your Android device to install: https://play.google.com/store/apps/details?id=com.Barlowax.resolutionfragments You can also find it on the Play Store by searching for the Keywords "Resolution Objective" |
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