Autofocus
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Did anybody try to use Nikon's autofocus (confocals) on Leica?
Thanks Axel Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. |
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How?
-----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Axel Kurt Preuss Sent: Monday, February 22, 2010 6:29 PM To: [hidden email] Subject: Autofocus Did anybody try to use Nikon's autofocus (confocals) on Leica? Thanks Axel Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. |
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In reply to this post by Axel Kurt Preuss
...sorry, pressed the send button by mistake... Could you clarify the question?
-----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Axel Kurt Preuss Sent: Monday, February 22, 2010 6:29 PM To: [hidden email] Subject: Autofocus Did anybody try to use Nikon's autofocus (confocals) on Leica? Thanks Axel Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. |
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Nikon seems to sell the best AF available for it's confocal line.
Colleagues of mine have it and are very pleased (day long time lapse) but I haven t met anybody who tried to transfer the AF onto other systems. Insurmountable? Thanks Greetings Axel IMCB-Central Imaging +65 9271 5622 On Feb 24, 2010, at 12:35 AM, "Vergara, Leoncio A." <[hidden email]> wrote: > ...sorry, pressed the send button by mistake... Could you clarify > the question? > > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of Axel Kurt > Preuss > Sent: Monday, February 22, 2010 6:29 PM > To: [hidden email] > Subject: Autofocus > > Did anybody try to use Nikon's autofocus (confocals) on Leica? > > Thanks > Axel > > Note: This message may contain confidential information. If this > Email/Fax has been sent to you by mistake, please notify the sender > and delete it immediately. Thank you. Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. |
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Nikon's Perfect Focus System is built into the nosepiece of the Ti
microscope so I think it's pretty much impossible to transfer it to another scope. Kurt Axel Kurt Preuss wrote: > Nikon seems to sell the best AF available for it's confocal line. > Colleagues of mine have it and are very pleased (day long time lapse) > but I haven t met anybody who tried to transfer the AF onto other > systems. Insurmountable? > > Thanks > > Greetings > > Axel > > IMCB-Central Imaging > +65 9271 5622 > > On Feb 24, 2010, at 12:35 AM, "Vergara, Leoncio A." > <[hidden email]> wrote: > > >> ...sorry, pressed the send button by mistake... Could you clarify >> the question? >> >> -----Original Message----- >> From: Confocal Microscopy List >> [mailto:[hidden email]] On Behalf Of Axel Kurt >> Preuss >> Sent: Monday, February 22, 2010 6:29 PM >> To: [hidden email] >> Subject: Autofocus >> >> Did anybody try to use Nikon's autofocus (confocals) on Leica? >> >> Thanks >> Axel >> >> Note: This message may contain confidential information. If this >> Email/Fax has been sent to you by mistake, please notify the sender >> and delete it immediately. Thank you. >> > > Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. > > |
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What do you mean by Nikon's autofocus (confocals)? Do you mean the focus drift control mechanism PFS - Perfect Focus System that is built in the nose piece of the Ti-E inverted microscope? The new generation PFS works by reflecting a semicircle of infrared light from an 870 nm LED off of the coverslip. The reflection is projected onto a linear CCD sensor in order to track the position of the coverslip. By combining this highly sensitive feedback system with the accurate Z-axis control of the Ti-E microscope, focusing precision of less than 1/3 the focal depth of the objective is easily achieved. Focus correction is achieved at a 5 ms sampling rate, making the PFS faster and less prone to error. PFS is not a plug and play add on module. As Kurt said, it is not possible to install this to other inverted systems. Any one knows the difference between the focus drift control mechanism of Olympus and Nikon? Roshama On Wed, Feb 24, 2010 at 6:47 AM, Kurt Thorn <[hidden email]> wrote: Nikon's Perfect Focus System is built into the nosepiece of the Ti microscope so I think it's pretty much impossible to transfer it to another scope. |
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you cannot even transfer the Nikon PF from the Ti and late TE2000 into older Nikon models like even the early TE2000... we missed the chance and now are stuck with a perfectly good scope but have no option to retrofit the PF whcih would make long term time lapse and multiwell screening much easier.
I understand the ZDC from Olympus is equivalent, we are exploring the possibility to retrofit it into an existing Olympus scope.... I wonder if Leica has come up with their own version, I think Zeiss did... The laser refelction autofocus is not exclusive from Nikon nor they invented it.... many high throughput microscopy systems also have a version of it or similar... (BD Pathways, Mol Devices) There was a publication from Ian Parker on TIRFM a while ago in which they describe a home made system, it predates the comercial release of Nikon's PF.... and there are other versions of similar custom systems published for stabilization of TIRFM/AFM combination scopes... so someone with a good working knowledge of electronics and photonics could probably easily build one... and tell us the secrets. I am wondering if there are third party companies developping solutions..... it would be the salvation for those like us .... I think 'ASI' has a system but only works with high NA oil lenses and aqueous mounted samples and uses a shorter wavelenght laser which precludes the use of dyes like Cy5.... 'Prior' also has components to develop a system but they have only explored it for non biological applications.... Leoncio Vergara ________________________________________ From: Confocal Microscopy List [[hidden email]] On Behalf Of Roshma Azeem [[hidden email]] Sent: Tuesday, February 23, 2010 9:43 PM To: [hidden email] Subject: Re: Autofocus What do you mean by Nikon's autofocus (confocals)? Do you mean the focus drift control mechanism PFS - Perfect Focus System that is built in the nose piece of the Ti-E inverted microscope? The new generation PFS works by reflecting a semicircle of infrared light from an 870 nm LED off of the coverslip. The reflection is projected onto a linear CCD sensor in order to track the position of the coverslip. By combining this highly sensitive feedback system with the accurate Z-axis control of the Ti-E microscope, focusing precision of less than 1/3 the focal depth of the objective is easily achieved. Focus correction is achieved at a 5 ms sampling rate, making the PFS faster and less prone to error. PFS is not a plug and play add on module. As Kurt said, it is not possible to install this to other inverted systems. Any one knows the difference between the focus drift control mechanism of Olympus and Nikon? Roshama On Wed, Feb 24, 2010 at 6:47 AM, Kurt Thorn <[hidden email]<mailto:[hidden email]>> wrote: Nikon's Perfect Focus System is built into the nosepiece of the Ti microscope so I think it's pretty much impossible to transfer it to another scope. Kurt Axel Kurt Preuss wrote: Nikon seems to sell the best AF available for it's confocal line. Colleagues of mine have it and are very pleased (day long time lapse) but I haven t met anybody who tried to transfer the AF onto other systems. Insurmountable? Thanks Greetings Axel IMCB-Central Imaging +65 9271 5622 On Feb 24, 2010, at 12:35 AM, "Vergara, Leoncio A." <[hidden email]<mailto:[hidden email]>> wrote: ...sorry, pressed the send button by mistake... Could you clarify the question? -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]<mailto:[hidden email]>] On Behalf Of Axel Kurt Preuss Sent: Monday, February 22, 2010 6:29 PM To: [hidden email]<mailto:[hidden email]> Subject: Autofocus Did anybody try to use Nikon's autofocus (confocals) on Leica? Thanks Axel Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. |
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Leica will come up with an equivalent, but you guys better ask them
All I can say is that the Nikon system is very affordable. Thanks Axel Central Imaging (IMCB) 6-19B, -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vergara, Leoncio A. Sent: Wednesday, February 24, 2010 4:05 PM To: [hidden email] Subject: Re: Autofocus you cannot even transfer the Nikon PF from the Ti and late TE2000 into older Nikon models like even the early TE2000... we missed the chance and now are stuck with a perfectly good scope but have no option to retrofit the PF whcih would make long term time lapse and multiwell screening much easier. I understand the ZDC from Olympus is equivalent, we are exploring the possibility to retrofit it into an existing Olympus scope.... I wonder if Leica has come up with their own version, I think Zeiss did... The laser refelction autofocus is not exclusive from Nikon nor they invented it.... many high throughput microscopy systems also have a version of it or similar... (BD Pathways, Mol Devices) There was a publication from Ian Parker on TIRFM a while ago in which they describe a home made system, it predates the comercial release of Nikon's PF.... and there are other versions of similar custom systems published for stabilization of TIRFM/AFM combination scopes... so someone with a good working knowledge of electronics and photonics could probably easily build one... and tell us the secrets. I am wondering if there are third party companies developping solutions..... it would be the salvation for those like us .... I think 'ASI' has a system but only works with high NA oil lenses and aqueous mounted samples and uses a shorter wavelenght laser which precludes the use of dyes like Cy5.... 'Prior' also has components to develop a system but they have only explored it for non biological applications.... Leoncio Vergara ________________________________________ From: Confocal Microscopy List [[hidden email]] On Behalf Of Roshma Azeem [[hidden email]] Sent: Tuesday, February 23, 2010 9:43 PM To: [hidden email] Subject: Re: Autofocus What do you mean by Nikon's autofocus (confocals)? Do you mean the focus drift control mechanism PFS - Perfect Focus System that is built in the nose piece of the Ti-E inverted microscope? The new generation PFS works by reflecting a semicircle of infrared light from an 870 nm LED off of the coverslip. The reflection is projected onto a linear CCD sensor in order to track the position of the coverslip. By combining this highly sensitive feedback system with the accurate Z-axis control of the Ti-E microscope, focusing precision of less than 1/3 the focal depth of the objective is easily achieved. Focus correction is achieved at a 5 ms sampling rate, making the PFS faster and less prone to error. PFS is not a plug and play add on module. As Kurt said, it is not possible to install this to other inverted systems. Any one knows the difference between the focus drift control mechanism of Olympus and Nikon? Roshama On Wed, Feb 24, 2010 at 6:47 AM, Kurt Thorn <[hidden email]<mailto:[hidden email]>> wrote: Nikon's Perfect Focus System is built into the nosepiece of the Ti microscope so I think it's pretty much impossible to transfer it to another scope. Kurt Axel Kurt Preuss wrote: Nikon seems to sell the best AF available for it's confocal line. Colleagues of mine have it and are very pleased (day long time lapse) but I haven t met anybody who tried to transfer the AF onto other systems. Insurmountable? Thanks Greetings Axel IMCB-Central Imaging +65 9271 5622 On Feb 24, 2010, at 12:35 AM, "Vergara, Leoncio A." <[hidden email]<mailto:[hidden email]>> wrote: ...sorry, pressed the send button by mistake... Could you clarify the question? -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]<mailto:[hidden email]>] On Behalf Of Axel Kurt Preuss Sent: Monday, February 22, 2010 6:29 PM To: [hidden email]<mailto:[hidden email]> Subject: Autofocus Did anybody try to use Nikon's autofocus (confocals) on Leica? Thanks Axel Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. |
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In reply to this post by Axel Kurt Preuss
The company WDI has released a system called LASS for live animal imaging stabilization based on the same principle as PSF from Nikon
Hope it will help ... 2010/2/22 Axel Kurt Preuss <[hidden email]> Did anybody try to use Nikon's autofocus (confocals) on Leica? -- Stéphane Pages PhD Neurobiologie cellulaire Centre de Recherche Université Laval Robert-Giffard (CRULRG) www.greenspine.ca Bureau/office: F-5570 2601, Chemin de la Canardière Beauport (Québec) G1J 2G3 Canada Tél: 418-663-5747 ext. 4712 (bur./office) Fax: 418-663-8756 |
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In reply to this post by leoncio vergara
Yes ASI does make a system that Leoncio
mentioned; it is based upon a TIRF principle
so it needs an objective with an N.A. of
1.45, or higher. Information is available
at http://www.asiimaging.com/criff.html ,
or please feel free to contact me directly;
:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=: " John Zemek [hidden email] : Applied Scientific Instrumentation " 29391 West Enid Road, Eugene, OR 97402-9533 USA : (541) 461-8181 x13 (800) 706-2284 US & Canada " (541) 461-4018 Fax http://www.ASIimaging.com :=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=: If this message has reached you in error or you would like to be taken off our mailing list, please reply to this e-mail and include "Unsubscribe" in the subject line. ----------------------- Original
Message -----------------------
From: "Vergara, Leoncio
A." [hidden email]
To: [hidden email]
Date: Wed, 24 Feb 2010 02:04:40
-0600
Subject: Re: Autofocus
you cannot even transfer the Nikon PF
from the Ti and late TE2000 into older Nikon
models like even the early TE2000... we missed
the chance and now are stuck with a perfectly
good scope but have no option to retrofit
the PF whcih would make long term time lapse
and multiwell screening much easier.
I understand the ZDC from Olympus is equivalent, we are exploring the possibility to retrofit it into an existing Olympus scope.... I wonder if Leica has come up with their own version, I think Zeiss did... The laser refelction autofocus is not exclusive from Nikon nor they invented it.... many high throughput microscopy systems also have a version of it or similar... (BD Pathways, Mol Devices) There was a publication from Ian Parker on TIRFM a while ago in which they describe a home made system, it predates the comercial release of Nikon's PF.... and there are other versions of similar custom systems published for stabilization of TIRFM/AFM combination scopes... so someone with a good working knowledge of electronics and photonics could probably easily build one... and tell us the secrets. I am wondering if there are third party companies developping solutions..... it would be the salvation for those like us .... I think 'ASI' has a system but only works with high NA oil lenses and aqueous mounted samples and uses a shorter wavelenght laser which precludes the use of dyes like Cy5.... 'Prior' also has components to develop a system but they have only explored it for non biological applications.... Leoncio Vergara ________________________________________ From: Confocal Microscopy List [hidden email] On Behalf Of Roshma Azeem [hidden email] Sent: Tuesday, February 23, 2010 9:43 PM To: [hidden email] Subject: Re: Autofocus What do you mean by Nikon's autofocus (confocals)? Do you mean the focus drift control mechanism PFS - Perfect Focus System that is built in the nose piece of the Ti-E inverted microscope? The new generation PFS works by reflecting a semicircle of infrared light from an 870 nm LED off of the coverslip. The reflection is projected onto a linear CCD sensor in order to track the position of the coverslip. By combining this highly sensitive feedback system with the accurate Z-axis control of the Ti-E microscope, focusing precision of less than 1/3 the focal depth of the objective is easily achieved. Focus correction is achieved at a 5 ms sampling rate, making the PFS faster and less prone to error. PFS is not a plug and play add on module. As Kurt said, it is not possible to install this to other inverted systems. Any one knows the difference between the focus drift control mechanism of Olympus and Nikon? Roshama On Wed, Feb 24, 2010 at 6:47 AM, Kurt Thorn <[hidden email]<mailto:[hidden email]>> wrote: Nikon's Perfect Focus System is built into the nosepiece of the Ti microscope so I think it's pretty much impossible to transfer it to another scope. Kurt Axel Kurt Preuss wrote: Nikon seems to sell the best AF available for it's confocal line. Colleagues of mine have it and are very pleased (day long time lapse) but I haven t met anybody who tried to transfer the AF onto other systems. Insurmountable? Thanks Greetings Axel IMCB-Central Imaging +65 9271 5622 On Feb 24, 2010, at 12:35 AM, "Vergara, Leoncio A." <[hidden email]<mailto:[hidden email]>> wrote: ...sorry, pressed the send button by mistake... Could you clarify the question? -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]<mailto:[hidden email]>] On Behalf Of Axel Kurt Preuss Sent: Monday, February 22, 2010 6:29 PM To: [hidden email]<mailto:[hidden email]> Subject: Autofocus Did anybody try to use Nikon's autofocus (confocals) on Leica? Thanks Axel Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you. |
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In reply to this post by leoncio vergara
Hi,
I have been looking for some Third-Party tools for my "ueber-scope" that I'm trying to order. It seems there are a few such companies that have (possibly needing some work for installation) solutions: 1) MadCityLabs C-focus system which can be controlled by micromanager or labview. http://www.madcitylabs.com/cfocus.html 2) Physik Instrumente offer a focus drift control as part of their P-737 pifoc specimen focussing z-stages http://www.physikinstrumente.com/en/products/prdetail.php?sortnr=201546 Hope this helps. Cheers, Chaitanya |
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