Best mid-range camera - Suggestions?

[Jens]

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Dear Listers,

I have to pick your brains again:

What would be your suggestion for a mid-range camera used mainly for fixed immunofluorescence and some stronger fluorescent protein labeling in living cells at mid speed (100 ms and up).
I would be especially interested if you would choose sCMOS over CCD here.

Thanks!

Jens

---------------------------------------------------------------
Dr. Jens Bernhard Bosse
Heinrich-Pette-Institut
Leibniz-Institut für Experimentelle Virologie
Martinistrasse 52
Hamburg 20251
Germany

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[Gerhard Holst]

Hi Jens,

That's a question for your application and your budget. How about the expected intra-scene dynamic (= contrast in one image). If you have a large difference between the brightest and the darkest spots in the image, and you are interested in resolving the structural information at that level, it might be a good idea to go for sCMOS (15 bit dynamic), as well, if you can foresee that you will investigate the dynamics in time, which can benefit from the higher frame rates.
If you have weak signals and look usually to steady state, means you expect long exposure times, and 12 bit intra-scene dynamic are ok, then you might look for a cooled CCD camera. (which can be in the same ballpark price like the sCMSO cameras.
If you have sufficiently bright samples and price tag is a major issue, you might either look into non-cooled CCD cameras or have a look to some of the new Sony IMX174AL based CMOS cameras in Machin vision. That's a 12 bit CMOS image sensor with good performance and due to machine vision applications with a reasonably low price tag.

Or if you could better specify you understanding of mid-range? You mentioned "... at mid speed (100 ms and up)." Does that mean 100 ms exposure time and longer, or does it mean 1/100ms frame rate = 10 images/s and faster? The first interpretation in my opinion would results in a cooled CCD, while the second in general goes for CMOS and sCMOS due to the speed requirement.

with best regards,

Gerhard
_______________________________

Dr. Gerhard Holst
Science & Research
PCO AG
Donaupark 11
93309 Kelheim, Germany
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-----Ursprüngliche Nachricht-----
Von: Confocal Microscopy List [mailto:[hidden email]] Im Auftrag von Jens-Bernhard Bosse
Gesendet: Mittwoch, 28. Oktober 2015 11:34
An: [hidden email]
Betreff: Best mid-range camera - Suggestions?

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Dear Listers,

I have to pick your brains again:

What would be your suggestion for a mid-range camera used mainly for fixed immunofluorescence and some stronger fluorescent protein labeling in living cells at mid speed (100 ms and up).
I would be especially interested if you would choose sCMOS over CCD here.

Thanks!

Jens

---------------------------------------------------------------
Dr. Jens Bernhard Bosse
Heinrich-Pette-Institut
Leibniz-Institut für Experimentelle Virologie
Martinistrasse 52
Hamburg 20251
Germany

[hidden email]


This electronic communication, including any attached documents, may contain confidential and/or legally privileged information that is intended only for use by the recipient(s) named above.  If you have received this communication in error, please notify the sender immediately and delete the communication and any attachments.

[Larisa Arena]

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Hi Jens,

pls have a look on science.duel.life. There is a broad and detailed
overview about cons/pro of sCMOS/CCD cams, as well as EMCCD and ICCD.

http://science.duel.life/index.php/compare-instruments/cameras

(Its a commercially independent source)

Best Regards, Larisa


Am 28.10.2015 um 11:33 schrieb Jens-Bernhard Bosse:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Listers,
>
> I have to pick your brains again:
>
> What would be your suggestion for a mid-range camera used mainly for fixed immunofluorescence and some stronger fluorescent protein labeling in living cells at mid speed (100 ms and up).
> I would be especially interested if you would choose sCMOS over CCD here.
>
> Thanks!
>
> Jens
>
> ---------------------------------------------------------------
> Dr. Jens Bernhard Bosse
> Heinrich-Pette-Institut
> Leibniz-Institut für Experimentelle Virologie
> Martinistrasse 52
> Hamburg 20251
> Germany
>
> [hidden email]
>
>
> This electronic communication, including any attached documents, may contain confidential and/or legally privileged information that is intended only for use by the recipient(s) named above.  If you have received this communication in error, please notify the sender immediately and delete the communication and any attachments.