Best stereomicroscope for GFP

[Caroline Bass]

Hey Guys,

I’m continuing my quest for a decent GFP microscope to visualize GFP in rat brain slices (from 200 um to 1 mm thick). Can someone recommend a very good stereomicroscope that I can try with different LED configurations? Here are some options I’m considering...

http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2615#3164

http://www.edmundoptics.com/onlinecatalog/displayproduct.cfm?productid=3250

Any suggestions? I once demoed the following microscope that worked fabulously, but for $40K I have to wait for a grant first. I’m hoping to come up with something decent in the meantime.

http://www.olympusamerica.com/seg_section/product.asp?product=1013

Any and all advice is appreciated. Right now I’d love to get the scope used off of ebay or through other sources.

Thanks!

Caroline

[Craig Brideau]

It was mentioned in another thread on this list, but a couple years
ago I bought an architectural lighting LED and stuck them on the side
of a microscope I found lying around with reasonable results.  Splurge
on the microscope, but for the LED illuminator you can go fairly cheap
if you are willing to do a bit of work yourself.  Another list member
tried my design out and it seemed to work for him as well.  I can't
recall what microscope he used though.  The LED company was Enfis.

Craig


On Mon, Apr 19, 2010 at 5:09 PM, Caroline Bass <[hidden email]> wrote:

> Hey Guys,
>
> I’m continuing my quest for a decent GFP microscope to visualize GFP in rat
> brain slices (from 200 um to 1 mm thick). Can someone recommend a very good
> stereomicroscope that I can try with different LED configurations? Here are
> some options I’m considering...
>
> http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2615#3164
>
> http://www.edmundoptics.com/onlinecatalog/displayproduct.cfm?productid=3250
>
> Any suggestions? I once demoed the following microscope that worked
> fabulously, but for $40K I have to wait for a grant first. I’m hoping to
> come up with something decent in the meantime.
>
> http://www.olympusamerica.com/seg_section/product.asp?product=1013
>
> Any and all advice is appreciated. Right now I’d love to get the scope used
> off of ebay or through other sources.
>
> Thanks!
>
> Caroline

[Gerard Whoriskey-3]

** Commercial Interest **

 

Please consider an LED solution from CoolLED.  We offer the widest range of LED wavelengths and believe that our products are the most intense and stable available.

 

The pE-100 can be specified for GFP.  It is more intense than a 100W Hg lamp at 470nm (optimum wavelength to suit most excitation filters).

 

See www.CoolLED.com

 

If you have any further questions, contact me off-server at [hidden email]

 

Thanks

 

Gerry / CoolLED

 

 

---

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Caroline Bass
Sent: 20 April 2010 00:09
To: [hidden email]
Subject: Best stereomicroscope for GFP

 

Hey Guys,

I’m continuing my quest for a decent GFP microscope to visualize GFP in rat brain slices (from 200 um to 1 mm thick). Can someone recommend a very good stereomicroscope that I can try with different LED configurations? Here are some options I’m considering...

http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2615#3164

http://www.edmundoptics.com/onlinecatalog/displayproduct.cfm?productid=3250

Any suggestions? I once demoed the following microscope that worked fabulously, but for $40K I have to wait for a grant first. I’m hoping to come up with something decent in the meantime.

http://www.olympusamerica.com/seg_section/product.asp?product=1013

Any and all advice is appreciated. Right now I’d love to get the scope used off of ebay or through other sources.

Thanks!

Caroline

 

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