Best way to join lexan for optical case

[Tim Feinstein]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hello all,

Quick question for those who have built a lexan enclosure for your scope
or beamline: what do you use to join the lexan panels?  I want to avoid
outgassing issues with the optics.  Alternatively I could do it the hard
way and use dovetail joints and metal L brackets to avoid glue altogether.
 With laser cutting that seems somewhat doable.  Your experiences
appreciated.  

Thanks and all the best,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology

[Mark Cannell-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Tim

I would have thought the outgassing of methylene chloride should be negligible after a short time. Failing that use epoxy? It is also possible to thermally weld lexan I believe.

HTH

Mark
On 27/07/2015, at 2:10 pm, Feinstein, Timothy N <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello all,
>
> Quick question for those who have built a lexan enclosure for your scope
> or beamline: what do you use to join the lexan panels?  I want to avoid
> outgassing issues with the optics.  Alternatively I could do it the hard
> way and use dovetail joints and metal L brackets to avoid glue altogether.
> With laser cutting that seems somewhat doable.  Your experiences
> appreciated.  
>
> Thanks and all the best,
>
>
> Tim
>
> Timothy Feinstein, Ph.D.
> Research Scientist
> University of Pittsburgh Department of Developmental Biology

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]

[Doug Koebler]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Tim,

I have used SciGrip (Weld-On) Cement for Acrylic from McMaster Carr.
http://www.mcmaster.com/#catalog/121/3440/=y8gqoc

First I tried to just paint on the adhesive, too many bubbles. Next I tries
a needle applicator, that worked.

There are many YouTube videos on this method, works fine.
https://www.youtube.com/watch?v=3KzZDi-aXD4

On all of my chambers on the microscope, I did screw the parts together (so
I could make changes!)

Doug



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Feinstein, Timothy N
Sent: Monday, July 27, 2015 9:11 AM
To: [hidden email]
Subject: Best way to join lexan for optical case

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hello all,

Quick question for those who have built a lexan enclosure for your scope or
beamline: what do you use to join the lexan panels?  I want to avoid
outgassing issues with the optics.  Alternatively I could do it the hard way
and use dovetail joints and metal L brackets to avoid glue altogether.
 With laser cutting that seems somewhat doable.  Your experiences
appreciated.  

Thanks and all the best,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology


---
This email has been checked for viruses by Avast antivirus software.
https://www.avast.com/antivirus

[Arvydas Matiukas]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****


Dear list,

One of the users of my confocal core complains that she sees
nearly perfect colocalization in the cells labeled by Lysotracker Red
(Ex 577, Em 590) and Mitotracker Deep Red (Ex 644, Em 665).
 
I suggested to additionally prepare and image unstained and single
stained cells. While unstained cells did not show autofluorescence,
and Lysotracker stained ones show low signal, it is weird that Mitotracker
labeled cells emit comparable signal  in both bands (571-651 and 651-755,
Ex by 561 and 633 nm lasers). My suspicion is that either cells are not
healthy or staining protocol is not working.
 
Please share your experience of simultaneously labeling cells with Lysotracker
and Mitotracker, or suggest alternatives.

Thanks and all the best,

Arvydas
 
 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University

[mcammer]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

We have had issues with the so called fixable mitotracker red and, therefore, image the cells while alive.  Admittedly, the problem could be in our fixation step, but the live imaging works.
=========================================================================
 Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
                      Cell:  914-309-3270     ** MY OFFICE HAS MOVED TO SKIRBALL 2nd FLOOR, Back right **
          http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Arvydas Matiukas
Sent: Monday, July 27, 2015 2:41 PM
To: [hidden email]
Subject: Lysotracker and Mitotracker staining

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****


Dear list,

One of the users of my confocal core complains that she sees nearly perfect colocalization in the cells labeled by Lysotracker Red (Ex 577, Em 590) and Mitotracker Deep Red (Ex 644, Em 665).
 
I suggested to additionally prepare and image unstained and single stained cells. While unstained cells did not show autofluorescence, and Lysotracker stained ones show low signal, it is weird that Mitotracker labeled cells emit comparable signal  in both bands (571-651 and 651-755, Ex by 561 and 633 nm lasers). My suspicion is that either cells are not healthy or staining protocol is not working.
 
Please share your experience of simultaneously labeling cells with Lysotracker and Mitotracker, or suggest alternatives.

Thanks and all the best,

Arvydas
 
 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University

------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
=================================