Calibration fibre
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Dear Listers,
Im looking for a rod/fibre equivalent to the fluorescence beads used for microscope calibration. As I would like to use these rods as a control for a skeletonization algorithm they need to be of a known length, preferably between 2 and 10µm and have a diameter of 0.5 - 1µm. It is also preferable that the rods are made of a material that is able to be bent or twisted. Any suggestions would be most appreciated Kind regards, Taryn |
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Taryn Saggese wrote:
> I’m looking for a rod/fibre equivalent to the fluorescence beads used for > microscope calibration. As I would like to use these rods as a control for a > skeletonization algorithm they need to be of a known length, preferably > between 2 and 10µm and have a diameter of 0.5 - 1µm. It is also preferable > that the rods are made of a material that is able to be bent or twisted. > > Any suggestions would be most appreciated Might diatoms work? They couldn't be twisted but some of those have interesting, convoluted shapes that have been pretty thoroughly characterized. Good luck-- Martin Wessendorf -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 **MY E-MAIL ADDRESS HAS CHANGED. PLEASE USE [hidden email] ** |
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In reply to this post by Taryn Saggese
You can buy fluorescent plastic fiber but it's relatively large diameter
(0.25 mm). You might be able to draw it to a finer diameter, or their may be distributors of smaller diameter material. Kurt Taryn Saggese wrote: > Dear Listers, > > I’m looking for a rod/fibre equivalent to the fluorescence beads used for > microscope calibration. As I would like to use these rods as a control for a > skeletonization algorithm they need to be of a known length, preferably > between 2 and 10µm and have a diameter of 0.5 - 1µm. It is also preferable > that the rods are made of a material that is able to be bent or twisted. > > Any suggestions would be most appreciated > > Kind regards, > Taryn > > -- Kurt Thorn, PhD Director, Nikon Imaging Center University of California San Francisco UCSF MC 2140 Genentech Hall Room S252 600 16th St. San Francisco, CA 94158-2517 http://nic.ucsf.edu phone 415.514.9709 fax 415.514.4300 |
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What is the source for this fiber? I'm curious about who makes and distributes it.
Thanks, Craig On Tue, Oct 21, 2008 at 10:45 AM, Kurt Thorn <[hidden email]> wrote: You can buy fluorescent plastic fiber but it's relatively large diameter (0.25 mm). You might be able to draw it to a finer diameter, or their may be distributors of smaller diameter material. |
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In reply to this post by Taryn Saggese
How about soaking a human hair in fluorescein? About 100 um. |
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In reply to this post by Taryn Saggese
Tayrn and other listserver users-
Our group (CISMM at UNC) routinely makes arrays of flexible rods about that size. We could probably make them fluorescent. Are there any other users out there that would find them useful? If there is a great need in the community, we could move it up our list, but could possibly make some for only a few users. We also write software for analyzing tube or rod shapes or movements so it might be interesting to compare notes on the algorithm you are testing. Tim O'Brien CISMM http://www.cs.unc.edu/Research/nano/cismm/ UNC Physics and Astronomy Chapel Hill, NC Taryn Saggese wrote: > Dear Listers, > > I’m looking for a rod/fibre equivalent to the fluorescence beads used for > microscope calibration. As I would like to use these rods as a control for a > skeletonization algorithm they need to be of a known length, preferably > between 2 and 10µm and have a diameter of 0.5 - 1µm. It is also preferable > that the rods are made of a material that is able to be bent or twisted. > > Any suggestions would be most appreciated > > Kind regards, > Taryn > |
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In reply to this post by Craig Brideau
If you google fluorescent fiber or wavelength shifting fiber you'll find
a bunch of vendors. Wavelength shifting fiber is apparently what the physics community refers to this stuff as - they use it for converting scintillator fluorescence to a longer wavelength to match PMT peak sensitivities - and this search turns up lots of papers. The distributors are mostly small hobby-oriented vendors. These guys appear to be one of the manufacturers: http://www.nanoptics.com/ Kurt Craig Brideau wrote: > What is the source for this fiber? I'm curious about who makes and > distributes it. > > Thanks, > > Craig > > > On Tue, Oct 21, 2008 at 10:45 AM, Kurt Thorn <[hidden email] > <mailto:[hidden email]>> wrote: > > You can buy fluorescent plastic fiber but it's relatively large > diameter (0.25 mm). You might be able to draw it to a finer > diameter, or their may be distributors of smaller diameter material. > > Kurt > > > Taryn Saggese wrote: > > Dear Listers, > I'm looking for a rod/fibre equivalent to the fluorescence > beads used for > microscope calibration. As I would like to use these rods as a > control for a > skeletonization algorithm they need to be of a known length, > preferably > between 2 and 10µm and have a diameter of 0.5 - 1µm. It is > also preferable > that the rods are made of a material that is able to be bent > or twisted. > Any suggestions would be most appreciated > Kind regards, Taryn > > > > > > -- > Kurt Thorn, PhD > Director, Nikon Imaging Center > University of California San Francisco > > UCSF MC 2140 > Genentech Hall Room S252 > 600 16th St. > San Francisco, CA 94158-2517 > > http://nic.ucsf.edu > phone 415.514.9709 > fax 415.514.4300 > > -- Kurt Thorn, PhD Director, Nikon Imaging Center University of California San Francisco UCSF MC 2140 Genentech Hall Room S252 600 16th St. San Francisco, CA 94158-2517 http://nic.ucsf.edu phone 415.514.9709 fax 415.514.4300 |
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In reply to this post by Martin Wessendorf-2
Why not try cotton fibres? They can be stained with many fluorescent
dyes, e.g. DAPI, propidium iodide, calcofluor white, in fact, almost any DNA or carbohydrate stain. They are a few microns in diameter, very flexible, centimeters long and very tough. Cotton wool from the chemist or supermarket should work fine. cheers, Rosemary Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia ph 61 2 6246 5475 fx 61 2 6246 5334 ________________________________________ From: Confocal Microscopy List [[hidden email]] On Behalf Of Martin Wessendorf [[hidden email]] Sent: Wednesday, 22 October 2008 12:45 a.m. To: [hidden email] Subject: Re: Calibration fibre Taryn Saggese wrote: > I’m looking for a rod/fibre equivalent to the fluorescence beads used for > microscope calibration. As I would like to use these rods as a control for a > skeletonization algorithm they need to be of a known length, preferably > between 2 and 10µm and have a diameter of 0.5 - 1µm. It is also preferable > that the rods are made of a material that is able to be bent or twisted. > > Any suggestions would be most appreciated Might diatoms work? They couldn't be twisted but some of those have interesting, convoluted shapes that have been pretty thoroughly characterized. Good luck-- Martin Wessendorf -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 **MY E-MAIL ADDRESS HAS CHANGED. PLEASE USE [hidden email] ** |
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Hmm, misread the dimensions.... Another option would be pollen, very
regular shapes, many are elongated, e.g. canola pollen, most are fairly autofluorescent, can be stained if desired and they're tough. Reasonably uniform size, often quite well-characterised, like diatoms. And unless you need a pure sample, kitty litter will give you lots of diatoms... though perhaps not of known length. ________________________________________ From: Confocal Microscopy List [[hidden email]] On Behalf Of [hidden email] [[hidden email]] Sent: Wednesday, 22 October 2008 7:43 a.m. To: [hidden email] Subject: Re: Calibration fibre Why not try cotton fibres? They can be stained with many fluorescent dyes, e.g. DAPI, propidium iodide, calcofluor white, in fact, almost any DNA or carbohydrate stain. They are a few microns in diameter, very flexible, centimeters long and very tough. Cotton wool from the chemist or supermarket should work fine. cheers, Rosemary Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia ph 61 2 6246 5475 fx 61 2 6246 5334 ________________________________________ From: Confocal Microscopy List [[hidden email]] On Behalf Of Martin Wessendorf [[hidden email]] Sent: Wednesday, 22 October 2008 12:45 a.m. To: [hidden email] Subject: Re: Calibration fibre Taryn Saggese wrote: > I’m looking for a rod/fibre equivalent to the fluorescence beads used for > microscope calibration. As I would like to use these rods as a control for a > skeletonization algorithm they need to be of a known length, preferably > between 2 and 10µm and have a diameter of 0.5 - 1µm. It is also preferable > that the rods are made of a material that is able to be bent or twisted. > > Any suggestions would be most appreciated Might diatoms work? They couldn't be twisted but some of those have interesting, convoluted shapes that have been pretty thoroughly characterized. Good luck-- Martin Wessendorf -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 **MY E-MAIL ADDRESS HAS CHANGED. PLEASE USE [hidden email] ** |
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In reply to this post by Kurt Thorn
Hi Taryn,
We routinely make fluorescent polystyrene 'nanofibers' of typically 1-5 micrometer diameter which are quite flexible. It should be possible to make them as thin as 500 nm. We use lasercutting to make shorter pieces of a few tens of nanometers in length. You can contact me if you would be interested in this. Best regards, Kevin Prof. Dr. Kevin Braeckmans Lab. General Biochemistry and Physical Pharmacy Ghent University Harelbekestraat 72 9000 Ghent Belgium Tel: +32 (0)9 264.80.78 Fax: +32 (0)9 264.81.89 > Taryn Saggese wrote: > > Dear Listers, > > > > Im looking for a rod/fibre equivalent to the fluorescence beads used > for > > microscope calibration. As I would like to use these rods as a > control for a > > skeletonization algorithm they need to be of a known length, > preferably > > between 2 and 10µm and have a diameter of 0.5 - 1µm. It is also > preferable > > that the rods are made of a material that is able to be bent or > twisted. > > > > Any suggestions would be most appreciated > > > > Kind regards, > > Taryn |
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