Camera issue
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear all, I’m reaching out as I have an issue with a camera on a widefield microscope (sorry, not confocal, but you may help): Following the upgrade of our computer to Win10 by Zeiss, the Hamamatsu Flash4 camera linked to our Observer has started behaving strangely. It is feeding images to the Zen software that do not correspond to what the microscope should see: if for example, we try to image DAPI, FITC and TRITC in a multichannel image, all channels look like DAPI. Yet, the microscope changes filters appropriately. It’s as if the camera buffer is not emptying itself and re-feeding an old image. Sometimes, these ghost images happen when there is no object on the stage. Drivers are up-to date and computer is running Zen 2.6. The Zeiss engineers are equally puzzled. Has anyone experienced this issue before and if so, what was your solution? Sincerely, Matthieu The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Check to see that you don't have any shading correction enabled. ------------------------------------------------------------------- Jason M. Kirk Technical Director, Optical Imaging & Vital Microscopy Core (OiVM) Baylor College of Medicine Ph: 713.798.6486 Email: [hidden email] http://www.bcm.edu/oivm -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of VERMEREN Matthieu Sent: Wednesday, January 13, 2021 9:45 AM To: [hidden email] Subject: Camera issue ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=yXnfAE8uMdZaMvW02fOtqH6i45jUz8tcrQTMV7z8wSk&m=DI_YuCNbHTyyWNTbU-ubGop3Kz1v6kvru_EDMKcLaQQ&s=S1xgXMXqMDCvu_Dg7yYwZnNVYAiDYm6M_gco839gzjk&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=yXnfAE8uMdZaMvW02fOtqH6i45jUz8tcrQTMV7z8wSk&m=DI_YuCNbHTyyWNTbU-ubGop3Kz1v6kvru_EDMKcLaQQ&s=Qn97UMauluY-_32IpLbjCEB7xC6sXfBlAp3nz_oA28k&e= and include the link in your posting. ***** Dear all, I’m reaching out as I have an issue with a camera on a widefield microscope (sorry, not confocal, but you may help): Following the upgrade of our computer to Win10 by Zeiss, the Hamamatsu Flash4 camera linked to our Observer has started behaving strangely. It is feeding images to the Zen software that do not correspond to what the microscope should see: if for example, we try to image DAPI, FITC and TRITC in a multichannel image, all channels look like DAPI. Yet, the microscope changes filters appropriately. It’s as if the camera buffer is not emptying itself and re-feeding an old image. Sometimes, these ghost images happen when there is no object on the stage. Drivers are up-to date and computer is running Zen 2.6. The Zeiss engineers are equally puzzled. Has anyone experienced this issue before and if so, what was your solution? Sincerely, Matthieu The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. |
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In reply to this post by VERMEREN Matthieu
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** To check if it’s really a camera issue I would install Micro-Manager and see how the camera behaves. For quick diagnosis you can install only the camera in MM and operate the microscope manually to check what the camera sees. -Esteban On Wed, Jan 13, 2021 at 7:50 AM VERMEREN Matthieu < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear all, > > I’m reaching out as I have an issue with a camera on a widefield > microscope (sorry, not confocal, but you may help): > > Following the upgrade of our computer to Win10 by Zeiss, the Hamamatsu > Flash4 camera linked to our Observer has started behaving strangely. It is > feeding images to the Zen software that do not correspond to what the > microscope should see: if for example, we try to image DAPI, FITC and TRITC > in a multichannel image, all channels look like DAPI. Yet, the microscope > changes filters appropriately. It’s as if the camera buffer is not > emptying itself and re-feeding an old image. Sometimes, these ghost images > happen when there is no object on the stage. Drivers are up-to date and > computer is running Zen 2.6. > > The Zeiss engineers are equally puzzled. > > Has anyone experienced this issue before and if so, what was your solution? > > Sincerely, > > Matthieu > The University of Edinburgh is a charitable body, registered in Scotland, > with registration number SC005336. > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I second the suggestion that Win10 has confused the communication between the camera and ZEN. I’d start by installing the Hamamatsu DCAM-API and then run ExCap, a bare-bones camera controller app that Hamamatsu offers to verify camera function. If you can see the correct image when controlling the scope manually and capturing with ExCap, try a capturing a multi-channel image with Micro-Manager. If that works then the problem is inside ZEN. All the best, T Timothy Feinstein, Ph.D. Research Scientist Department of Developmental Biology University of Pittsburgh On 1/13/21, 10:58 AM, "Confocal Microscopy List on behalf of G. Esteban Fernandez" <[hidden email] on behalf of [hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gnumKQfW2bQGpjxoovjMazx5n6N0amYz%2FIC8t9Jc5bU%3D&reserved=0 Post images on https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=r84edUnX%2BK5QttvQhQZAohPhoaJI2NdUKfSFL9DSiF8%3D&reserved=0 and include the link in your posting. ***** To check if it’s really a camera issue I would install Micro-Manager and see how the camera behaves. For quick diagnosis you can install only the camera in MM and operate the microscope manually to check what the camera sees. -Esteban On Wed, Jan 13, 2021 at 7:50 AM VERMEREN Matthieu < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gnumKQfW2bQGpjxoovjMazx5n6N0amYz%2FIC8t9Jc5bU%3D&reserved=0 > Post images on https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=r84edUnX%2BK5QttvQhQZAohPhoaJI2NdUKfSFL9DSiF8%3D&reserved=0 and include the link in your posting. > ***** > > Dear all, > > I’m reaching out as I have an issue with a camera on a widefield > microscope (sorry, not confocal, but you may help): > > Following the upgrade of our computer to Win10 by Zeiss, the Hamamatsu > Flash4 camera linked to our Observer has started behaving strangely. It is > feeding images to the Zen software that do not correspond to what the > microscope should see: if for example, we try to image DAPI, FITC and TRITC > in a multichannel image, all channels look like DAPI. Yet, the microscope > changes filters appropriately. It’s as if the camera buffer is not > emptying itself and re-feeding an old image. Sometimes, these ghost images > happen when there is no object on the stage. Drivers are up-to date and > computer is running Zen 2.6. > > The Zeiss engineers are equally puzzled. > > Has anyone experienced this issue before and if so, what was your solution? > > Sincerely, > > Matthieu > The University of Edinburgh is a charitable body, registered in Scotland, > with registration number SC005336. > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I would definitely follow the suggestions of others to try it with ExCap and MM. You say "Hamamatsu Flash4 camera linked to our Observer", so does it use Camera Link (or rather USB)? If it is Camera Link indeed and the problem persists with MM I would check which exactly frame-grabber you have - maybe it does not get along with Win 10. good luck, Tomasz Tomasz Wegierski, PhD International Institute of Molecular and Cell Biology Trojdena 4, 02-109 Warsaw, POLAND tel: +48-22 597 0763 fax: +48 22 597 0715 http://www.iimcb.gov.pl/ ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Feinstein, Timothy N <[hidden email]> Sent: Wednesday, January 13, 2021 5:11 PM To: [hidden email] <[hidden email]> Subject: Re: Camera issue ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I second the suggestion that Win10 has confused the communication between the camera and ZEN. I’d start by installing the Hamamatsu DCAM-API and then run ExCap, a bare-bones camera controller app that Hamamatsu offers to verify camera function. If you can see the correct image when controlling the scope manually and capturing with ExCap, try a capturing a multi-channel image with Micro-Manager. If that works then the problem is inside ZEN. All the best, T Timothy Feinstein, Ph.D. Research Scientist Department of Developmental Biology University of Pittsburgh On 1/13/21, 10:58 AM, "Confocal Microscopy List on behalf of G. Esteban Fernandez" <[hidden email] on behalf of [hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gnumKQfW2bQGpjxoovjMazx5n6N0amYz%2FIC8t9Jc5bU%3D&reserved=0 Post images on https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=r84edUnX%2BK5QttvQhQZAohPhoaJI2NdUKfSFL9DSiF8%3D&reserved=0 and include the link in your posting. ***** To check if it’s really a camera issue I would install Micro-Manager and see how the camera behaves. For quick diagnosis you can install only the camera in MM and operate the microscope manually to check what the camera sees. -Esteban On Wed, Jan 13, 2021 at 7:50 AM VERMEREN Matthieu < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gnumKQfW2bQGpjxoovjMazx5n6N0amYz%2FIC8t9Jc5bU%3D&reserved=0 > Post images on https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=r84edUnX%2BK5QttvQhQZAohPhoaJI2NdUKfSFL9DSiF8%3D&reserved=0 and include the link in your posting. > ***** > > Dear all, > > I’m reaching out as I have an issue with a camera on a widefield > microscope (sorry, not confocal, but you may help): > > Following the upgrade of our computer to Win10 by Zeiss, the Hamamatsu > Flash4 camera linked to our Observer has started behaving strangely. It is > feeding images to the Zen software that do not correspond to what the > microscope should see: if for example, we try to image DAPI, FITC and TRITC > in a multichannel image, all channels look like DAPI. Yet, the microscope > changes filters appropriately. It’s as if the camera buffer is not > emptying itself and re-feeding an old image. Sometimes, these ghost images > happen when there is no object on the stage. Drivers are up-to date and > computer is running Zen 2.6. > > The Zeiss engineers are equally puzzled. > > Has anyone experienced this issue before and if so, what was your solution? > > Sincerely, > > Matthieu > The University of Edinburgh is a charitable body, registered in Scotland, > with registration number SC005336. > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** One more thing came to my mind. If the camera works just fine under ExCap and MM, and it looks that ZEN is guilty: Was MTB upgraded to a newer version during Win 10 install? I have no idea whether MTB version can affect the communication with a 3rd party camera (AFAIK: the proper version of MTB, sometimes pretty old one, may be important for the 3rd party software to talk to Zeiss components) but it would be worth checking. You could install the older version of MTB that worked with your camera previously (without uninstalling current one), disable the newer MTB server and manually start the older version MTB server, start ZEN and check whether camera starts to work properly. Tomasz Wegierski, PhD International Institute of Molecular and Cell Biology Trojdena 4, 02-109 Warsaw, POLAND tel: +48-22 597 0763 fax: +48 22 597 0715 http://www.iimcb.gov.pl/ ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Tomek Węgierski <[hidden email]> Sent: Wednesday, January 13, 2021 8:25 PM To: [hidden email] <[hidden email]> Subject: Re: Camera issue ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I would definitely follow the suggestions of others to try it with ExCap and MM. You say "Hamamatsu Flash4 camera linked to our Observer", so does it use Camera Link (or rather USB)? If it is Camera Link indeed and the problem persists with MM I would check which exactly frame-grabber you have - maybe it does not get along with Win 10. good luck, Tomasz Tomasz Wegierski, PhD International Institute of Molecular and Cell Biology Trojdena 4, 02-109 Warsaw, POLAND tel: +48-22 597 0763 fax: +48 22 597 0715 http://www.iimcb.gov.pl/ ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Feinstein, Timothy N <[hidden email]> Sent: Wednesday, January 13, 2021 5:11 PM To: [hidden email] <[hidden email]> Subject: Re: Camera issue ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I second the suggestion that Win10 has confused the communication between the camera and ZEN. I’d start by installing the Hamamatsu DCAM-API and then run ExCap, a bare-bones camera controller app that Hamamatsu offers to verify camera function. If you can see the correct image when controlling the scope manually and capturing with ExCap, try a capturing a multi-channel image with Micro-Manager. If that works then the problem is inside ZEN. All the best, T Timothy Feinstein, Ph.D. Research Scientist Department of Developmental Biology University of Pittsburgh On 1/13/21, 10:58 AM, "Confocal Microscopy List on behalf of G. Esteban Fernandez" <[hidden email] on behalf of [hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gnumKQfW2bQGpjxoovjMazx5n6N0amYz%2FIC8t9Jc5bU%3D&reserved=0 Post images on https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=r84edUnX%2BK5QttvQhQZAohPhoaJI2NdUKfSFL9DSiF8%3D&reserved=0 and include the link in your posting. ***** To check if it’s really a camera issue I would install Micro-Manager and see how the camera behaves. For quick diagnosis you can install only the camera in MM and operate the microscope manually to check what the camera sees. -Esteban On Wed, Jan 13, 2021 at 7:50 AM VERMEREN Matthieu < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gnumKQfW2bQGpjxoovjMazx5n6N0amYz%2FIC8t9Jc5bU%3D&reserved=0 > Post images on https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=r84edUnX%2BK5QttvQhQZAohPhoaJI2NdUKfSFL9DSiF8%3D&reserved=0 and include the link in your posting. > ***** > > Dear all, > > I’m reaching out as I have an issue with a camera on a widefield > microscope (sorry, not confocal, but you may help): > > Following the upgrade of our computer to Win10 by Zeiss, the Hamamatsu > Flash4 camera linked to our Observer has started behaving strangely. It is > feeding images to the Zen software that do not correspond to what the > microscope should see: if for example, we try to image DAPI, FITC and TRITC > in a multichannel image, all channels look like DAPI. Yet, the microscope > changes filters appropriately. It’s as if the camera buffer is not > emptying itself and re-feeding an old image. Sometimes, these ghost images > happen when there is no object on the stage. Drivers are up-to date and > computer is running Zen 2.6. > > The Zeiss engineers are equally puzzled. > > Has anyone experienced this issue before and if so, what was your solution? > > Sincerely, > > Matthieu > The University of Edinburgh is a charitable body, registered in Scotland, > with registration number SC005336. > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Been a while since i worked with Zen blue, but there used to be several settings related to the buffering/clearing of images for third party cameras that could be changed. Settings were inside the software not the MTB As you suggest it sounds like it is either 1. Not sequencing the camera chip clearing with the microscope movements, so essentially you see only the first channel 2. Not clearing the images between channels at all, so you image is all channels. I cannot remember what the setting was called sorry, its been over 5 years since i worked with them. ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Tomek Węgierski <[hidden email]> Sent: Thursday, January 14, 2021 7:24 AM To: [hidden email] <[hidden email]> Subject: Re: Camera issue ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** One more thing came to my mind. If the camera works just fine under ExCap and MM, and it looks that ZEN is guilty: Was MTB upgraded to a newer version during Win 10 install? I have no idea whether MTB version can affect the communication with a 3rd party camera (AFAIK: the proper version of MTB, sometimes pretty old one, may be important for the 3rd party software to talk to Zeiss components) but it would be worth checking. You could install the older version of MTB that worked with your camera previously (without uninstalling current one), disable the newer MTB server and manually start the older version MTB server, start ZEN and check whether camera starts to work properly. Tomasz Wegierski, PhD International Institute of Molecular and Cell Biology Trojdena 4, 02-109 Warsaw, POLAND tel: +48-22 597 0763 fax: +48 22 597 0715 http://www.iimcb.gov.pl/ ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Tomek Węgierski <[hidden email]> Sent: Wednesday, January 13, 2021 8:25 PM To: [hidden email] <[hidden email]> Subject: Re: Camera issue ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I would definitely follow the suggestions of others to try it with ExCap and MM. You say "Hamamatsu Flash4 camera linked to our Observer", so does it use Camera Link (or rather USB)? If it is Camera Link indeed and the problem persists with MM I would check which exactly frame-grabber you have - maybe it does not get along with Win 10. good luck, Tomasz Tomasz Wegierski, PhD International Institute of Molecular and Cell Biology Trojdena 4, 02-109 Warsaw, POLAND tel: +48-22 597 0763 fax: +48 22 597 0715 http://www.iimcb.gov.pl/ ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Feinstein, Timothy N <[hidden email]> Sent: Wednesday, January 13, 2021 5:11 PM To: [hidden email] <[hidden email]> Subject: Re: Camera issue ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I second the suggestion that Win10 has confused the communication between the camera and ZEN. I’d start by installing the Hamamatsu DCAM-API and then run ExCap, a bare-bones camera controller app that Hamamatsu offers to verify camera function. If you can see the correct image when controlling the scope manually and capturing with ExCap, try a capturing a multi-channel image with Micro-Manager. If that works then the problem is inside ZEN. All the best, T Timothy Feinstein, Ph.D. Research Scientist Department of Developmental Biology University of Pittsburgh On 1/13/21, 10:58 AM, "Confocal Microscopy List on behalf of G. Esteban Fernandez" <[hidden email] on behalf of [hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gnumKQfW2bQGpjxoovjMazx5n6N0amYz%2FIC8t9Jc5bU%3D&reserved=0 Post images on https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=r84edUnX%2BK5QttvQhQZAohPhoaJI2NdUKfSFL9DSiF8%3D&reserved=0 and include the link in your posting. ***** To check if it’s really a camera issue I would install Micro-Manager and see how the camera behaves. For quick diagnosis you can install only the camera in MM and operate the microscope manually to check what the camera sees. -Esteban On Wed, Jan 13, 2021 at 7:50 AM VERMEREN Matthieu < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gnumKQfW2bQGpjxoovjMazx5n6N0amYz%2FIC8t9Jc5bU%3D&reserved=0 > Post images on https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Ctnf8%40PITT.EDU%7C1ce4363229d44a305d5a08d8b7dbfe03%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C637461502808819490%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=r84edUnX%2BK5QttvQhQZAohPhoaJI2NdUKfSFL9DSiF8%3D&reserved=0 and include the link in your posting. > ***** > > Dear all, > > I’m reaching out as I have an issue with a camera on a widefield > microscope (sorry, not confocal, but you may help): > > Following the upgrade of our computer to Win10 by Zeiss, the Hamamatsu > Flash4 camera linked to our Observer has started behaving strangely. It is > feeding images to the Zen software that do not correspond to what the > microscope should see: if for example, we try to image DAPI, FITC and TRITC > in a multichannel image, all channels look like DAPI. Yet, the microscope > changes filters appropriately. It’s as if the camera buffer is not > emptying itself and re-feeding an old image. Sometimes, these ghost images > happen when there is no object on the stage. Drivers are up-to date and > computer is running Zen 2.6. > > The Zeiss engineers are equally puzzled. > > Has anyone experienced this issue before and if so, what was your solution? > > Sincerely, > > Matthieu > The University of Edinburgh is a charitable body, registered in Scotland, > with registration number SC005336. > |
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