Cleaning of CSU W1 spinning disk scan head

[Pascal Lorentz-2]

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Dear list

We have a CSU W1 spinning disk scan head and most likely a dust particle
on the disk that creates a well defined line in the image.
Please find an image here: https://imgur.com/a/e3NKg
Did somebody ever clean the disks successfully? I heard that it is very
tricky to do on site in a non dust free environment.
How would I need to proceed if I would like to clean it myself? How can
I access the disks and are there any tips and tricks on how to prevent
even more dust flying onto the disks. So far I just accessed the
emission filter wheels and the dichroic mirrors but did not dare to
access the disks. Any manuals would be helpful.

Thanks a lot for your help and best regards

Pascal

[leavesley]

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Hi Pascal,

Just a thought - aren't these units usually assembled in a clean room?

Best regards,

Silas


On 1/12/2018 9:12 AM, Pascal Lorentz wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear list
>
> We have a CSU W1 spinning disk scan head and most likely a dust
> particle on the disk that creates a well defined line in the image.
> Please find an image here: https://imgur.com/a/e3NKg
> Did somebody ever clean the disks successfully? I heard that it is
> very tricky to do on site in a non dust free environment.
> How would I need to proceed if I would like to clean it myself? How
> can I access the disks and are there any tips and tricks on how to
> prevent even more dust flying onto the disks. So far I just accessed
> the emission filter wheels and the dichroic mirrors but did not dare
> to access the disks. Any manuals would be helpful.
>
> Thanks a lot for your help and best regards
>
> Pascal

--
Silas J. Leavesley, Ph.D.
Professor
Department of Chemical and Biomolecular Engineering
Department of Pharmacology
Center for Lung Biology
University of South Alabama
150 Jaguar Drive, SH4129
Mobile, AL 36688
ph: (251)-460-6160
fax: [phone]
web: http://www.southalabama.edu/centers/bioimaging
google scholar: http://scholar.google.co.uk/citations?user=knkwcj4AAAAJ
------------------------------------------------------------------------

[Jens]

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Same here. The dust problem with the W1 is a major pain. I would love to hear about fixes.

Jens

> Am 12.01.2018 um 16:48 schrieb Silas Leavesley <[hidden email]>:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Pascal,
>
> Just a thought - aren't these units usually assembled in a clean room?
>
> Best regards,
>
> Silas
>
>
>> On 1/12/2018 9:12 AM, Pascal Lorentz wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear list
>>
>> We have a CSU W1 spinning disk scan head and most likely a dust particle on the disk that creates a well defined line in the image.
>> Please find an image here: https://imgur.com/a/e3NKg
>> Did somebody ever clean the disks successfully? I heard that it is very tricky to do on site in a non dust free environment.
>> How would I need to proceed if I would like to clean it myself? How can I access the disks and are there any tips and tricks on how to prevent even more dust flying onto the disks. So far I just accessed the emission filter wheels and the dichroic mirrors but did not dare to access the disks. Any manuals would be helpful.
>>
>> Thanks a lot for your help and best regards
>>
>> Pascal
>
> --
> Silas J. Leavesley, Ph.D.
> Professor
> Department of Chemical and Biomolecular Engineering
> Department of Pharmacology
> Center for Lung Biology
> University of South Alabama
> 150 Jaguar Drive, SH4129
> Mobile, AL 36688
> ph: (251)-460-6160
> fax: [phone]
> web: http://www.southalabama.edu/centers/bioimaging
> google scholar: http://scholar.google.co.uk/citations?user=knkwcj4AAAAJ
> ------------------------------------------------------------------------

[John Oreopoulos]

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This issue was discussed last month on the confocal listserver as  
well. Search the archives.

John Oreopoulos

Quoting Jens Bernhard Bosse <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Same here. The dust problem with the W1 is a major pain. I would  
> love to hear about fixes.
>
> Jens
>
>
>> Am 12.01.2018 um 16:48 schrieb Silas Leavesley <[hidden email]>:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi Pascal,
>>
>> Just a thought - aren't these units usually assembled in a clean room?
>>
>> Best regards,
>>
>> Silas
>>
>>
>>> On 1/12/2018 9:12 AM, Pascal Lorentz wrote:
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your posting.
>>> *****
>>>
>>> Dear list
>>>
>>> We have a CSU W1 spinning disk scan head and most likely a dust  
>>> particle on the disk that creates a well defined line in the image.
>>> Please find an image here: https://imgur.com/a/e3NKg
>>> Did somebody ever clean the disks successfully? I heard that it is  
>>>  very tricky to do on site in a non dust free environment.
>>> How would I need to proceed if I would like to clean it myself?  
>>> How can I access the disks and are there any tips and tricks on  
>>> how to prevent even more dust flying onto the disks. So far I just  
>>>  accessed the emission filter wheels and the dichroic mirrors but  
>>> did not dare to access the disks. Any manuals would be helpful.
>>>
>>> Thanks a lot for your help and best regards
>>>
>>> Pascal
>>
>> --
>> Silas J. Leavesley, Ph.D.
>> Professor
>> Department of Chemical and Biomolecular Engineering
>> Department of Pharmacology
>> Center for Lung Biology
>> University of South Alabama
>> 150 Jaguar Drive, SH4129
>> Mobile, AL 36688
>> ph: (251)-460-6160
>> fax: [phone]
>> web: http://www.southalabama.edu/centers/bioimaging
>> google scholar: http://scholar.google.co.uk/citations?user=knkwcj4AAAAJ
>> ------------------------------------------------------------------------
>

[Ferhan A]

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Dear Pascal,

We have exactly the same headache with our dust gathering CSU W1 spinning
disk unit which started to produce images resembling the ones in the cover
pages of dendrochronology journals. As discussed earlier in this forum,
this seems to be a design flaw of W1 system. We will be very happy if the
original manufacturer comes up with a no-cost-to-customer fix to this
problem and offers free dust cleanup and reimburses us for the aspirins.
Best regards,
Ferhan
-------
Ferhan Ayaydin, Ph.D.
Cellular Imaging Laboratory
Biological Research Center
Hungarian Academy of Sciences
Temesvari krt. 62
6726 Szeged
Hungary


On Jan 12, 2018 16:23, "Pascal Lorentz" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear list
>
> We have a CSU W1 spinning disk scan head and most likely a dust particle
> on the disk that creates a well defined line in the image.
> Please find an image here: https://imgur.com/a/e3NKg
> Did somebody ever clean the disks successfully? I heard that it is very
> tricky to do on site in a non dust free environment.
> How would I need to proceed if I would like to clean it myself? How can I
> access the disks and are there any tips and tricks on how to prevent even
> more dust flying onto the disks. So far I just accessed the emission filter
> wheels and the dichroic mirrors but did not dare to access the disks. Any
> manuals would be helpful.
>
> Thanks a lot for your help and best regards
>
> Pascal
>

[Csúcs Gábor-3]

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*****

Dear All,

Though I fully agree with Ferhan that it would be good if either the manufacturer or the distributor would offer a solution to this dust problem, perhaps I can  make two suggestions that will certainly not eliminate the problem but perhaps make it less frequent (less frequent cleaning required). The first one is reduction of the dust in the room (very obvious). This can be potentially achieved by installing filters in the air-conditioning unit (if possible) and to apply some other "tricks" used in clean environments (sticky floor tapes etc.). The other suggestion: I believe (of course I have no scientific proof for this) that box-type incubators (enclosures) also may help. But again this does not solve the issue....

Greetings Gabor


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ferhan A
Sent: Saturday, January 13, 2018 9:58 AM
To: [hidden email]
Subject: Re: Cleaning of CSU W1 spinning disk scan head

*****
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*****

Dear Pascal,

We have exactly the same headache with our dust gathering CSU W1 spinning disk unit which started to produce images resembling the ones in the cover pages of dendrochronology journals. As discussed earlier in this forum, this seems to be a design flaw of W1 system. We will be very happy if the original manufacturer comes up with a no-cost-to-customer fix to this problem and offers free dust cleanup and reimburses us for the aspirins.
Best regards,
Ferhan
-------
Ferhan Ayaydin, Ph.D.
Cellular Imaging Laboratory
Biological Research Center
Hungarian Academy of Sciences
Temesvari krt. 62
6726 Szeged
Hungary


On Jan 12, 2018 16:23, "Pascal Lorentz" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear list
>
> We have a CSU W1 spinning disk scan head and most likely a dust
> particle on the disk that creates a well defined line in the image.
> Please find an image here: https://imgur.com/a/e3NKg Did somebody ever
> clean the disks successfully? I heard that it is very tricky to do on
> site in a non dust free environment.
> How would I need to proceed if I would like to clean it myself? How
> can I access the disks and are there any tips and tricks on how to
> prevent even more dust flying onto the disks. So far I just accessed
> the emission filter wheels and the dichroic mirrors but did not dare
> to access the disks. Any manuals would be helpful.
>
> Thanks a lot for your help and best regards
>
> Pascal
>

[Oliver Biehlmaier-2]

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*****

Dear all, dear Pascal,

This is indeed very annoying!
As some of you might remember from the conversation that Kai started last year asking about the experiences with current spinning disk systems, the reports on dust problems on the W1 were by far the most frequent.
I wonder how the manufacturer can get away with such a problem without being obliged to replace or at least fix those dust collecting systems?!

Pascal, I thought that your system already was “fixed” with the magic tape solution…. So apparently this did not help. Is that correct?

Did anyone of you guys purchase a new W1 within the past 6 months? According to the manufacturer and the local distributers the dust problem should be fixed on those recently sold W1 systems. Can anyone confirm this?

Concerning the problem described I would really try to ask the distributer to solve this, in particular if the system is still under warranty.
I have no idea how to clean the disk but the system should clearly only be opened and cleaned in a clean room. Otherwise Gabor’s solutions should at least reduce the dust problem.

Best regards,
Oliver



Oliver Biehlmaier, PhD | Head of Imaging Core Facility  | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel
Phone: [phone] | Email: [hidden email]<mailto:[hidden email]> | www.biozentrum.unibas.ch/imcf<http://www.biozentrum.unibas.ch/imcf> | www.microscopynetwork.unibas.ch<http://www.microscopynetwork.unibas.ch>








On 15 Jan 2018, at 09:51, Csúcs Gábor <[hidden email]<mailto:[hidden email]>> wrote:

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Dear All,

Though I fully agree with Ferhan that it would be good if either the manufacturer or the distributor would offer a solution to this dust problem, perhaps I can  make two suggestions that will certainly not eliminate the problem but perhaps make it less frequent (less frequent cleaning required). The first one is reduction of the dust in the room (very obvious). This can be potentially achieved by installing filters in the air-conditioning unit (if possible) and to apply some other "tricks" used in clean environments (sticky floor tapes etc.). The other suggestion: I believe (of course I have no scientific proof for this) that box-type incubators (enclosures) also may help. But again this does not solve the issue....

Greetings Gabor


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ferhan A
Sent: Saturday, January 13, 2018 9:58 AM
To: [hidden email]
Subject: Re: Cleaning of CSU W1 spinning disk scan head

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Pascal,

We have exactly the same headache with our dust gathering CSU W1 spinning disk unit which started to produce images resembling the ones in the cover pages of dendrochronology journals. As discussed earlier in this forum, this seems to be a design flaw of W1 system. We will be very happy if the original manufacturer comes up with a no-cost-to-customer fix to this problem and offers free dust cleanup and reimburses us for the aspirins.
Best regards,
Ferhan
-------
Ferhan Ayaydin, Ph.D.
Cellular Imaging Laboratory
Biological Research Center
Hungarian Academy of Sciences
Temesvari krt. 62
6726 Szeged
Hungary


On Jan 12, 2018 16:23, "Pascal Lorentz" <[hidden email]> wrote:

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Dear list

We have a CSU W1 spinning disk scan head and most likely a dust
particle on the disk that creates a well defined line in the image.
Please find an image here: https://imgur.com/a/e3NKg Did somebody ever
clean the disks successfully? I heard that it is very tricky to do on
site in a non dust free environment.
How would I need to proceed if I would like to clean it myself? How
can I access the disks and are there any tips and tricks on how to
prevent even more dust flying onto the disks. So far I just accessed
the emission filter wheels and the dichroic mirrors but did not dare
to access the disks. Any manuals would be helpful.

Thanks a lot for your help and best regards

Pascal

[Sylvie Le Guyader]

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Hi everyone

I also heard from a Yokogawa vendor that the problem was solved for the newer units but this is nothing official so I just wrote to Yokogawa to point to them that they should give an official answer to the confocal server list and also ask them the questions directly. If they answer to me but not to the list, I will forward their answer to everyone.

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7,
Novum, G lift, floor 6
14157 Huddinge
Sweden
mobile: [phone]
office: [phone]
LCI website


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Oliver Biehlmaier
Sent: den 15 januari 2018 11:17
To: [hidden email]
Subject: Re: Cleaning of CSU W1 spinning disk scan head

*****
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Dear all, dear Pascal,

This is indeed very annoying!
As some of you might remember from the conversation that Kai started last year asking about the experiences with current spinning disk systems, the reports on dust problems on the W1 were by far the most frequent.
I wonder how the manufacturer can get away with such a problem without being obliged to replace or at least fix those dust collecting systems?!

Pascal, I thought that your system already was “fixed” with the magic tape solution…. So apparently this did not help. Is that correct?

Did anyone of you guys purchase a new W1 within the past 6 months? According to the manufacturer and the local distributers the dust problem should be fixed on those recently sold W1 systems. Can anyone confirm this?

Concerning the problem described I would really try to ask the distributer to solve this, in particular if the system is still under warranty.
I have no idea how to clean the disk but the system should clearly only be opened and cleaned in a clean room. Otherwise Gabor’s solutions should at least reduce the dust problem.

Best regards,
Oliver



Oliver Biehlmaier, PhD | Head of Imaging Core Facility  | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel
Phone: [phone] | Email: [hidden email]<mailto:[hidden email]> | www.biozentrum.unibas.ch/imcf<http://www.biozentrum.unibas.ch/imcf> | www.microscopynetwork.unibas.ch<http://www.microscopynetwork.unibas.ch>








On 15 Jan 2018, at 09:51, Csúcs Gábor <[hidden email]<mailto:[hidden email]>> wrote:

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Dear All,

Though I fully agree with Ferhan that it would be good if either the manufacturer or the distributor would offer a solution to this dust problem, perhaps I can  make two suggestions that will certainly not eliminate the problem but perhaps make it less frequent (less frequent cleaning required). The first one is reduction of the dust in the room (very obvious). This can be potentially achieved by installing filters in the air-conditioning unit (if possible) and to apply some other "tricks" used in clean environments (sticky floor tapes etc.). The other suggestion: I believe (of course I have no scientific proof for this) that box-type incubators (enclosures) also may help. But again this does not solve the issue....

Greetings Gabor


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ferhan A
Sent: Saturday, January 13, 2018 9:58 AM
To: [hidden email]
Subject: Re: Cleaning of CSU W1 spinning disk scan head

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Pascal,

We have exactly the same headache with our dust gathering CSU W1 spinning disk unit which started to produce images resembling the ones in the cover pages of dendrochronology journals. As discussed earlier in this forum, this seems to be a design flaw of W1 system. We will be very happy if the original manufacturer comes up with a no-cost-to-customer fix to this problem and offers free dust cleanup and reimburses us for the aspirins.
Best regards,
Ferhan
-------
Ferhan Ayaydin, Ph.D.
Cellular Imaging Laboratory
Biological Research Center
Hungarian Academy of Sciences
Temesvari krt. 62
6726 Szeged
Hungary


On Jan 12, 2018 16:23, "Pascal Lorentz" <[hidden email]> wrote:

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Dear list

We have a CSU W1 spinning disk scan head and most likely a dust particle on the disk that creates a well defined line in the image.
Please find an image here: https://imgur.com/a/e3NKg Did somebody ever clean the disks successfully? I heard that it is very tricky to do on site in a non dust free environment.
How would I need to proceed if I would like to clean it myself? How can I access the disks and are there any tips and tricks on how to prevent even more dust flying onto the disks. So far I just accessed the emission filter wheels and the dichroic mirrors but did not dare to access the disks. Any manuals would be helpful.

Thanks a lot for your help and best regards

Pascal

[Emmanuel Gustin]

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Hi,

When we had dust problems with our spinning disk system, a colleague drew my attention to the following publication:
https://www.ncbi.nlm.nih.gov/pubmed/23507575

The cotton lab coats that many people prefer have a major disadvantage: The fibers are fluorescent. We had a significant reduction in the need to clean our spinning disk system by banning cotton lab coats from the imaging lab. The alternatives tend to be less comfortable (and less environmentally friendly) but are worth considering.

Of course, dust still gets into our optics, but it's fluorescent dust that is responsible for the very visible ring pattern artifacts.

Best Regards,

Emmanuel


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Csúcs Gábor
Sent: Monday, 15 January 2018 09:51
To: [hidden email]
Subject: [EXTERNAL] Re: Cleaning of CSU W1 spinning disk scan head

*****
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*****

Dear All,

Though I fully agree with Ferhan that it would be good if either the manufacturer or the distributor would offer a solution to this dust problem, perhaps I can  make two suggestions that will certainly not eliminate the problem but perhaps make it less frequent (less frequent cleaning required). The first one is reduction of the dust in the room (very obvious). This can be potentially achieved by installing filters in the air-conditioning unit (if possible) and to apply some other "tricks" used in clean environments (sticky floor tapes etc.). The other suggestion: I believe (of course I have no scientific proof for this) that box-type incubators (enclosures) also may help. But again this does not solve the issue....

Greetings Gabor


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ferhan A
Sent: Saturday, January 13, 2018 9:58 AM
To: [hidden email]
Subject: Re: Cleaning of CSU W1 spinning disk scan head

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Pascal,

We have exactly the same headache with our dust gathering CSU W1 spinning disk unit which started to produce images resembling the ones in the cover pages of dendrochronology journals. As discussed earlier in this forum, this seems to be a design flaw of W1 system. We will be very happy if the original manufacturer comes up with a no-cost-to-customer fix to this problem and offers free dust cleanup and reimburses us for the aspirins.
Best regards,
Ferhan
-------
Ferhan Ayaydin, Ph.D.
Cellular Imaging Laboratory
Biological Research Center
Hungarian Academy of Sciences
Temesvari krt. 62
6726 Szeged
Hungary


On Jan 12, 2018 16:23, "Pascal Lorentz" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear list
>
> We have a CSU W1 spinning disk scan head and most likely a dust
> particle on the disk that creates a well defined line in the image.
> Please find an image here: https://imgur.com/a/e3NKg Did somebody ever
> clean the disks successfully? I heard that it is very tricky to do on
> site in a non dust free environment.
> How would I need to proceed if I would like to clean it myself? How
> can I access the disks and are there any tips and tricks on how to
> prevent even more dust flying onto the disks. So far I just accessed
> the emission filter wheels and the dichroic mirrors but did not dare
> to access the disks. Any manuals would be helpful.
>
> Thanks a lot for your help and best regards
>
> Pascal
>

[George Peeters-2]

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Solamere Technology Group Inc has been dealing with Yokogawa products since 1996. Contrary to rumor, we did not sell our CSUX or CSU-W1 distributor-ship to Nikon.  (We did provide Nikon with customized CSUX & W1 Scan Heads for nearly 20 months ending in August 2016.) While a surprise from Japan, the ending of our supply to Nikon USA was amicable.

 

The  CSU-W1 has several compartments:

1)   The Disk Compartment houses the spinning disk pairs and the relay optics which pass the expanded, collimated laser beam to the micro-lens disk and the returning fluorescent image from the objective through the pin-hole disk.

2)   Filter wheel and emission/image Compartment is located in the larger rear section:  the image (of the pin hole disk) is re-collimated and relayed thru the emission Filter-wheel compartment. Depending on the configuration purchased , you may have two split emission paths passing thru two filter wheels, and on to a camera lens tubes for each camera.

3)   Electronics Compartment: The electronic drive circuit board needed to control the motors and actuators is located in a bottom compartment that has a fan to cool the component.  Air is drawn in through a filter and circulates over the boards.

 

 

The often reported CSU-W1 problems with dust adhering to the disks and short pass dichroics can be made worse by third party add-ons which open the disk compartment to increased airflow.

 

Since it introduced the CSU-W1, Yokogawa has made design changes to mitigate the issue, including providing air sealed adaptors for coupling the scan head to the microscope’s camera ports. If these are removed to facilitate adding  a bellows or other extensions, the dust problems can worsen as more air is circulated thru the compartment.

 

Cleaning can be done in the field, but a low dust environment is recommended. Wiping down all surfaces and limiting air movement around the scan head is necessary before opening any of the compartments on the W1. As often is the case, training and experience is helpful…. We have a had W1 scan heads in the field for approximately 4 years now and have had few reported problems with dust interfering with the images.  We have cleaned a few dichroics and have done some major cleaning on systems installed by other suppliers, most of these units had the sealed coupling adaptor removed. Like rust never sleeps, dust never settles…except on your optics. So cleaning is going to be required at some point. How often depends on your set up.


George Peeters, M.D., M.S.
Pres. Solamere Technology Group inc.
1515 Military Way
Salt Lake City, UT.  84103

Cell    801 232-6911

> On Jan 15, 2018, at 7:42 AM, Gustin, Emmanuel [JRDBE] <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> When we had dust problems with our spinning disk system, a colleague drew my attention to the following publication:
> https://www.ncbi.nlm.nih.gov/pubmed/23507575
>
> The cotton lab coats that many people prefer have a major disadvantage: The fibers are fluorescent. We had a significant reduction in the need to clean our spinning disk system by banning cotton lab coats from the imaging lab. The alternatives tend to be less comfortable (and less environmentally friendly) but are worth considering.
>
> Of course, dust still gets into our optics, but it's fluorescent dust that is responsible for the very visible ring pattern artifacts.
>
> Best Regards,
>
> Emmanuel
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Csúcs Gábor
> Sent: Monday, 15 January 2018 09:51
> To: [hidden email]
> Subject: [EXTERNAL] Re: Cleaning of CSU W1 spinning disk scan head
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> Though I fully agree with Ferhan that it would be good if either the manufacturer or the distributor would offer a solution to this dust problem, perhaps I can  make two suggestions that will certainly not eliminate the problem but perhaps make it less frequent (less frequent cleaning required). The first one is reduction of the dust in the room (very obvious). This can be potentially achieved by installing filters in the air-conditioning unit (if possible) and to apply some other "tricks" used in clean environments (sticky floor tapes etc.). The other suggestion: I believe (of course I have no scientific proof for this) that box-type incubators (enclosures) also may help. But again this does not solve the issue....
>
> Greetings Gabor
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ferhan A
> Sent: Saturday, January 13, 2018 9:58 AM
> To: [hidden email]
> Subject: Re: Cleaning of CSU W1 spinning disk scan head
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Pascal,
>
> We have exactly the same headache with our dust gathering CSU W1 spinning disk unit which started to produce images resembling the ones in the cover pages of dendrochronology journals. As discussed earlier in this forum, this seems to be a design flaw of W1 system. We will be very happy if the original manufacturer comes up with a no-cost-to-customer fix to this problem and offers free dust cleanup and reimburses us for the aspirins.
> Best regards,
> Ferhan
> -------
> Ferhan Ayaydin, Ph.D.
> Cellular Imaging Laboratory
> Biological Research Center
> Hungarian Academy of Sciences
> Temesvari krt. 62
> 6726 Szeged
> Hungary
>
>
> On Jan 12, 2018 16:23, "Pascal Lorentz" <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear list
>>
>> We have a CSU W1 spinning disk scan head and most likely a dust
>> particle on the disk that creates a well defined line in the image.
>> Please find an image here: https://imgur.com/a/e3NKg Did somebody ever
>> clean the disks successfully? I heard that it is very tricky to do on
>> site in a non dust free environment.
>> How would I need to proceed if I would like to clean it myself? How
>> can I access the disks and are there any tips and tricks on how to
>> prevent even more dust flying onto the disks. So far I just accessed
>> the emission filter wheels and the dichroic mirrors but did not dare
>> to access the disks. Any manuals would be helpful.
>>
>> Thanks a lot for your help and best regards
>>
>> Pascal
>>

[Pascal Lorentz-2]

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Dear all

Thanks a lot for all the feedback and tips. Unfortunately I did not
receive a cleaning advice so far that would allow me to clean the disks
myself. Please see my specific answers below.

@Silas:
Yes I think these units are assembled in a clean room but obviously not
super well sealed.

@John:
I am aware of the discussion last month but this did not deal with how
to clean the unit.

@Gabor:
Thanks a lot for the tips about filters for the air-conditioning and
sticky floor tapes. I will check with the building services what we can do.

@Oliver:
The magic tape your are referring to has been there since the
installation and does seal one of the slits between the lid and the
housing. As far as I know this has been installed by the distributor to
prevent the dust problem. But obviously this does not help completely.
There are a lot of other slits where dust can potentially enter. I am
also not sure if the dust is to some extend created within the unit
itself by abrasion due to the disk rotation.
The distributor already said that they are able to clean the disks even
if it will be tricky to do it in the field. However my attempt was that
I can maybe do it myself with a proper manual.
I probably have to wait until the distributor comes to clean it. I will
then of course have an eye on it to learn how to clean it properly. I
can then come over and clean yours if you still dare to buy one ;-)

@Sylvie:
Thanks a lot. I did not contact Yokogawa directly so far. So please let
me know if you get an answer from them.

@Emmanuel:
Good to know that lab coats fibers are fluorescent. However our users
usually don't wear these if they are sitting at the microscope.

@Georges
Thanks for the detailed description about the scan head. Do you maybe
have more details on how to access the disks and how to clean them?

Best regards

Pascal


Am 12.01.2018 um 16:48 schrieb Silas Leavesley:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Pascal,
>
> Just a thought - aren't these units usually assembled in a clean room?
>
> Best regards,
>
> Silas
>
>
> On 1/12/2018 9:12 AM, Pascal Lorentz wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your
>> posting.
>> *****
>>
>> Dear list
>>
>> We have a CSU W1 spinning disk scan head and most likely a dust
>> particle on the disk that creates a well defined line in the image.
>> Please find an image here: https://imgur.com/a/e3NKg
>> Did somebody ever clean the disks successfully? I heard that it is
>> very tricky to do on site in a non dust free environment.
>> How would I need to proceed if I would like to clean it myself? How
>> can I access the disks and are there any tips and tricks on how to
>> prevent even more dust flying onto the disks. So far I just accessed
>> the emission filter wheels and the dichroic mirrors but did not dare
>> to access the disks. Any manuals would be helpful.
>>
>> Thanks a lot for your help and best regards
>>
>> Pascal
>

[Jared K. Buks]

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I also have this issue on my CSU W1.  Andor came in (while under warranty) and cleaned it once for me, however it has reoccurred already in less than a years time.  Clearly this is a design issue as I still have my "tape" on the system yet dust readily finds its way inside.  I have had two other spinning disks and never had an issue with dust on them, so I am a touch perplexed as to why this keeps happening on the W1.  Anyway, this is going to sound crazy, but the Andor engineer wrapped chopsticks in lens paper soaked in EtOH and gently wiped the disk till the dust was removed.  He informed me this was the "standard" way to clean the disk.  I was more than a bit surprised by this method, but it appeared to have worked and the system was under warranty.  Disk was dust free for at least for 4-5 months.  Anyone else used this method?

Thanks

Jared

Jared K. Burks, PhD
Assistant Professor
Co-Director, Flow Cytometry & Cellular Imaging Core Facility
Department of Leukemia
M.D. Anderson Cancer Center Unit 0425
1515 Holcombe Blvd. Y8.5311a
Houston, TX  77030
713-792-6592

[leavesley]

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Hi Jared,

That's actually similar to how I clean optics, especially hard to reach
crevices/curves/etc.  I prefer to use a cotton swab (the ones with
wooden stems are easiest to manipulate).  I wrap it in 2 sheets of lens
paper.  I usually prep a decent number of swabs this way before
beginning so I don't run out.  Then just practice good cleaning
procedures and make sure to select an appropriate solvent for the task.  
FYI, I've never tried to clean the disk in any spinning disk myself,
just more mundane filters, objectives that students have submerged into
who knows what, etc.  Now that you've brought it up, it would be nice if
there were some video tutorials or something on different approaches to
optics cleaning for microscopes - maybe there are and I just haven't
seen them.

Best regards,

Silas


On 1/17/2018 9:26 AM, Jared K. Buks wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I also have this issue on my CSU W1.  Andor came in (while under warranty) and cleaned it once for me, however it has reoccurred already in less than a years time.  Clearly this is a design issue as I still have my "tape" on the system yet dust readily finds its way inside.  I have had two other spinning disks and never had an issue with dust on them, so I am a touch perplexed as to why this keeps happening on the W1.  Anyway, this is going to sound crazy, but the Andor engineer wrapped chopsticks in lens paper soaked in EtOH and gently wiped the disk till the dust was removed.  He informed me this was the "standard" way to clean the disk.  I was more than a bit surprised by this method, but it appeared to have worked and the system was under warranty.  Disk was dust free for at least for 4-5 months.  Anyone else used this method?
>
> Thanks
>
> Jared
>
> Jared K. Burks, PhD
> Assistant Professor
> Co-Director, Flow Cytometry & Cellular Imaging Core Facility
> Department of Leukemia
> M.D. Anderson Cancer Center Unit 0425
> 1515 Holcombe Blvd. Y8.5311a
> Houston, TX  77030
> 713-792-6592

--
Silas J. Leavesley, Ph.D.
Professor
Department of Chemical and Biomolecular Engineering
Department of Pharmacology
Center for Lung Biology
University of South Alabama
150 Jaguar Drive, SH4129
Mobile, AL 36688
ph: (251)-460-6160
fax: [phone]
web: http://www.southalabama.edu/centers/bioimaging
google scholar: http://scholar.google.co.uk/citations?user=knkwcj4AAAAJ
------------------------------------------------------------------------

[Nico Stuurman-3]

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On 1/17/18 11:49 AM, Silas Leavesley wrote:
> Now that you've brought it up, it would be nice if there were some
> video tutorials or something on different approaches to optics
> cleaning for microscopes - maybe there are and I just haven't seen them.

Here is one:

https://www.ibiology.org/talks/clean-objective-lenses/


No doubt, there are others...

Best,

Nico

[WAINWRIGHT James]

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*** SEMI-COMMERCIAL POSTING ***

Hi Jared (and all),

As an ex-service engineer for Andor (I still work for Andor, but in a different role), I can confirm the "chopstick" method was recommended to us by Yokogawa. We were equally amused and impressed!

My preferred cleaning tool(s) however are Texwipes (no commercial interest). For example:

https://www.texwipe.com/alpha-tx761-2

Having cleaned both CSU-X and CSU-W systems when we used to supply them, I can say it's surprisingly easy (if a little scary the first time!), although I'd rather not provide explicit instructions. The CSU-W is more complicated to clean as the disk(s) are less accessible.

In my experience, the CSU-22 was the least likely to accrue dust because it was such an enclosed system. The CSU-W is on the opposite end because it's so open.

Best wishes,

James

James Wainwright
Global Applications Specialist - Microscopy Systems

Tel:        [phone] ext. 2130
Skype:  andor.j.wainwright
Mob:     [phone]
Web:     https://www.andor.com/microscopy-systems



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jared K. Buks
Sent: 17 January 2018 15:26
To: [hidden email]
Subject: -|EXT|- Re: Cleaning of CSU W1 spinning disk scan head

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I also have this issue on my CSU W1.  Andor came in (while under warranty) and cleaned it once for me, however it has reoccurred already in less than a years time.  Clearly this is a design issue as I still have my "tape" on the system yet dust readily finds its way inside.  I have had two other spinning disks and never had an issue with dust on them, so I am a touch perplexed as to why this keeps happening on the W1.  Anyway, this is going to sound crazy, but the Andor engineer wrapped chopsticks in lens paper soaked in EtOH and gently wiped the disk till the dust was removed.  He informed me this was the "standard" way to clean the disk.  I was more than a bit surprised by this method, but it appeared to have worked and the system was under warranty.  Disk was dust free for at least for 4-5 months.  Anyone else used this method?

Thanks

Jared

Jared K. Burks, PhD
Assistant Professor
Co-Director, Flow Cytometry & Cellular Imaging Core Facility Department of Leukemia M.D. Anderson Cancer Center Unit 0425
1515 Holcombe Blvd. Y8.5311a
Houston, TX  77030
713-792-6592


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[Manabu Fueki]

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Dear all who use Yokogawa CSU-W1

Thank you for letting us know about your experience with our products.
We apologize for the inconvenience our product caused you.

For safety, we are not able to allow users to open the CSU unit. And working by yourself can cause damage the optical parts.
If you are in dust trouble of CSU-W1, please ask your distributor to clean and conduct the measures against dust.

From my experience, there are a lot of molds in the air from ducts of air conditioner or something, when CSU has dust issue. There are a lot of dust inside CSU already. So the unit need to clean inside. We'll open and do this countermeasure in a clean booth.

We'll take this action as soon as possible with our distributors.
We will do our best to improve the quality of our products and service.

Best regards,

Manabu

********************************************************************
Manabu Fueki
Yokogawa Electric Corporation
Measurement Business HQ
Life Science Center, QA & Customer Support Department
2-3 Hokuyodai, Kanazawa-shi, Ishikawa, 920-0177 Japan
Tel:[phone]  Fax:[phone]
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