Colibri vs HXP
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all I was considering buying a zeiss colibri system instead of a HXP system, any comments in comparing both systems? Any suggestion would be very helpful, Thanks a lot, Claudia. Claudia Florindo, PhD, Microscopy Unit Manager <mailto:[hidden email]%20%7C00351> [hidden email] |00351 289244 495 ext: 7495; 7489; 7484 (mic lab) Dept. de Ciências Biomedicas e Medicina Uni. Algarve, Campus de Gambelas Ed 8, lab 1.18 8005-139 Faro, Portugal. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We have a number of systems with both Colibri and HXP light sources, we still use the HXP for red and far red dyes. Not enough power in the LEDs. -Doug On Fri, Mar 1, 2013 at 11:05 AM, Claudia Florindo <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > > > Dear all > > > > I was considering buying a zeiss colibri system instead of a HXP system, > any > comments in comparing both systems? > > Any suggestion would be very helpful, > > > > Thanks a lot, > > > > Claudia. > > > > > > Claudia Florindo, PhD, Microscopy Unit Manager > > <mailto:[hidden email]%20%7C00351> [hidden email] |00351 289244 495 > ext: 7495; 7489; 7484 (mic lab) > > Dept. de Ciências Biomedicas e Medicina > > Uni. Algarve, Campus de Gambelas Ed 8, lab 1.18 > > 8005-139 Faro, Portugal. > > > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Doug, Witch version of the colibri are you talking about? Best, Nuno Moreno, PhD Instituto Gulbenkian de Ciência On Mar 1, 2013, at 5:19 PM, Douglas Richardson wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > We have a number of systems with both Colibri and HXP light sources, we > still use the HXP for red and far red dyes. Not enough power in the LEDs. > > -Doug > > On Fri, Mar 1, 2013 at 11:05 AM, Claudia Florindo <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> >> >> Dear all >> >> >> >> I was considering buying a zeiss colibri system instead of a HXP system, >> any >> comments in comparing both systems? >> >> Any suggestion would be very helpful, >> >> >> >> Thanks a lot, >> >> >> >> Claudia. >> >> >> >> >> >> Claudia Florindo, PhD, Microscopy Unit Manager >> >> <mailto:[hidden email]%20%7C00351> [hidden email] |00351 289244 495 >> ext: 7495; 7489; 7484 (mic lab) >> >> Dept. de Ciências Biomedicas e Medicina >> >> Uni. Algarve, Campus de Gambelas Ed 8, lab 1.18 >> >> 8005-139 Faro, Portugal. >> >> >> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We have both I and II. The red LEDs in the II are better, but still not as good as the HXP.according to our users. I have yet to do a head to head comparison myself. -Doug On Fri, Mar 1, 2013 at 12:48 PM, Nuno Moreno <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear Doug, > > Witch version of the colibri are you talking about? > > Best, > Nuno Moreno, PhD > Instituto Gulbenkian de Ciência > > > > > > On Mar 1, 2013, at 5:19 PM, Douglas Richardson wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > We have a number of systems with both Colibri and HXP light sources, we > > still use the HXP for red and far red dyes. Not enough power in the > LEDs. > > > > -Doug > > > > On Fri, Mar 1, 2013 at 11:05 AM, Claudia Florindo <[hidden email]> > wrote: > > > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> ***** > >> > >> > >> > >> Dear all > >> > >> > >> > >> I was considering buying a zeiss colibri system instead of a HXP system, > >> any > >> comments in comparing both systems? > >> > >> Any suggestion would be very helpful, > >> > >> > >> > >> Thanks a lot, > >> > >> > >> > >> Claudia. > >> > >> > >> > >> > >> > >> Claudia Florindo, PhD, Microscopy Unit Manager > >> > >> <mailto:[hidden email]%20%7C00351> [hidden email] |00351 289244 > 495 > >> ext: 7495; 7489; 7484 (mic lab) > >> > >> Dept. de Ciências Biomedicas e Medicina > >> > >> Uni. Algarve, Campus de Gambelas Ed 8, lab 1.18 > >> > >> 8005-139 Faro, Portugal. > >> > >> > >> > |
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In reply to this post by Claudia Florindo
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** HI Claudia, We like Metal Halide lamps. We have no experience with the Zeiss model. We use primarily the Xcite, but I am guessing they should be similar (or maybe even the same Zeiss sells). Benefits of Metal Halide: Broad spectrum with good brightness across; if you need to image a new dye, you just need to buy a new filter set Long life (typically 2,000 hours, we often run ours 3,000-4,000 hours, which can mean 1-2 years before changing bulbs, depending on how much you use your scope) Easy installation (no alignment needed) Many of the current models include shutters and variable apertures, and can be computer controlled if needed. Potential downside is that as filters get old, some light (including UV) may leak through, but this can be adressed by replacing filters or adding UV/IR blocks. Also, if lamp is turned off, next person will need to wait before getting it back on. No such problem with LEDs. However, current models have built-in safety; lamp won't fire if still hot. We have two LED or LED-like systems which work great for us; our models have either five or seven excitation bands, which works fine for the particular scopes we use, and they offer plenty of power at all wavelengths. They are not Colibris, and I don't know the specs of Colibri, so can't comment on the power. The fact that Colibri has only four wavelengths could be limiting... it all depends on what you intend to do on your microscope. The pros of LED are: Long life (rated 10,000 hours of actual use, which means they should last maybe ten years, although this may bee too new a technology to really know), fast switching, no UV leakage (useful for live imaging), and no need to replace/align bulbs. The fast switching between wavelengths can be a plus for live imaging (such as for FRET or colocalization), because you can use for instance a double band pass emission filter and image two channels without any moving parts (better speed, potentially better registration between channels). Intensity can also be software controlled. Vendors claim better image contrast.... could be true, but hard to know. The choice between metal halide and LED may depend on what the intended purpose of the microscope is, and perhaps cost (initial cost plus operational cost over the lifetime of the device). If your LED device has all the wavelengths you need (and enough power), I don't really see any downsides. Our LED-based systems provide at least 20 mW at any wavelength (measured at the focal plane with a 10x objective). That's roughly comparable to metal halide. We like the Xcite Metal halide because we can also open/close shutter and control illumination intensity manually. I don't know how Zeiss implements their Colibri, i.e. is it purely software controlled, or can you also control it manually (e.g. from the Zeiss control pad on the microscope)? Ultimately, Assuming that the Colibri covers your needs in terms of wavelengths available and/or power (which I would suggest you test), you'll be fine with either. If you expect to do five or more channels on your scope, then a Colibri with four excitation bands may not be what you want, and a Metal Halide may be more flexible/versatile. -- Julio Vazquez, Fred Hutchinson Cancer Research Center Seattle, United States http://www.fhcrc.org/ On Mar 1, 2013, at 8:05 AM, Claudia Florindo wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > > > Dear all > > > > I was considering buying a zeiss colibri system instead of a HXP system, any > comments in comparing both systems? > > Any suggestion would be very helpful, > > > > Thanks a lot, > > > > Claudia. > > > > > > Claudia Florindo, PhD, Microscopy Unit Manager > > <mailto:[hidden email]%20%7C00351> [hidden email] |00351 289244 495 > ext: 7495; 7489; 7484 (mic lab) > > Dept. de Ciências Biomedicas e Medicina > > Uni. Algarve, Campus de Gambelas Ed 8, lab 1.18 > > 8005-139 Faro, Portugal. > > |
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In reply to this post by Claudia Florindo
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** One more thing to mention about metal halide: light is typically delivered via a light guide, which may cost around $ 500. Sometimes they go bad and need to be replaced, so this is also a factor to consider. Our light guides typically last a good five years if we are careful (they don't like to be bent, and they don't like to get hot). -- Julio Vazquez Fred Hutchinson Cancer Research Center Seattle, USA http://www.fhcrc.org/ On Mar 1, 2013, at 8:05 AM, Claudia Florindo wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > > > Dear all > > > > I was considering buying a zeiss colibri system instead of a HXP system, any > comments in comparing both systems? > > Any suggestion would be very helpful, > > > > Thanks a lot, > > > > Claudia. > > > > > > Claudia Florindo, PhD, Microscopy Unit Manager > > <mailto:[hidden email]%20%7C00351> [hidden email] |00351 289244 495 > ext: 7495; 7489; 7484 (mic lab) > > Dept. de Ciências Biomedicas e Medicina > > Uni. Algarve, Campus de Gambelas Ed 8, lab 1.18 > > 8005-139 Faro, Portugal. > > |
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In reply to this post by Julio Vazquez
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Anyone tried http://www.coolled.com/Life-Sciences-Analytical/Products/pE-2/ ? ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Julio Vazquez Sent: Monday, March 04, 2013 12:02 PM To: [hidden email] Subject: Re: Colibri vs HXP ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** |
 
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George McNamara |
Re: Colibri vs HXP ... liquid light guide lifetime, recommended length, model, tips
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In reply to this post by Julio Vazquez
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** At ABRF 2013, the Lumen Dynamics manager (Mic C.) told me that the liquid light guides have about a 3 year shelf life (all the LLG's are from one manufacturer: Lumatec). That is, they will "go bad" even in a drawer. So, congratulations on getting five years (though you may have a bubble without knowing it). His tips included: * when storing, humid environment is much better than dry (it is liquid inside). * kinks are bad. * if the output (aimed at a white, uniform surface) is not a clean, completely uniform disk, you have a bubble (or the LLG into the lamp is not in correctly). I have recommendations for labs and suppliers: * a right angle turn adapter for the LLG to MICROSCOPE ... a lot of microscopes have a back wall very close - current "bend the LLG" is a good way to damage it. * "label" the LLG with tape (or paint?) to make it obvious that this is not a power cord. * if the microscope configuration allows, use a ONE METER LLG instead of the more common 2 meter. * Lumatec manufactures several modesl, with different transmission spectra. Last time I checked (a couple of years ago), the LLG-380 (1 meter) was the most sensible choice. *** As for the Colibri vs HXP ... The Zeiss Colibri was expensive when it was introduced in 2007. Zeiss is likely to lower prices only if their competitors are successful selling at lower prices. If you want a Colibri, find out if they offer new (brighter, more available colors) LED's7: the LED field has progressed a lot since 2007. HXP: we are in 2013: don't waste your money on metal halide, Hg, or Xe lamps when "solid state" lighting is now available. In Houston, I will have a Lumencor SOLA on "my" Leica AM6000 microscope (DMI 6000 stand with 2 Eppendork micromanipulators). If I have an opportunity to upgrade soon, it would probably be to the Lumencor Spectra X (6 or 7 independent wavelengths, though pricey ... I have access to an almost identical product, InsightSSI, on the GE/API OMX upstairs for deconvolution and interference reflection contrast microscopy) or Lumen's 4 wavelength X-LED product (and possibly by an alternative LED or two for special needs ... of course if someone wants to donate some money to the lab, I'll be happy to put two X-LED's (8 wavelengths) on the AM6000 scope. Sincerely, George On 3/4/2013 12:07 PM, Julio Vazquez wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > One more thing to mention about metal halide: light is typically delivered via a light guide, which may cost around $ 500. Sometimes they go bad and need to be replaced, so this is also a factor to consider. Our light guides typically last a good five years if we are careful (they don't like to be bent, and they don't like to get hot). > -- > Julio Vazquez > Fred Hutchinson Cancer Research Center > Seattle, USA > > http://www.fhcrc.org/ > > > > On Mar 1, 2013, at 8:05 AM, Claudia Florindo wrote: > > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> >> >> Dear all >> >> >> >> I was considering buying a zeiss colibri system instead of a HXP system, any >> comments in comparing both systems? >> >> Any suggestion would be very helpful, >> >> >> >> Thanks a lot, >> >> >> >> Claudia. >> >> >> >> >> >> Claudia Florindo, PhD, Microscopy Unit Manager >> >> <mailto:[hidden email]%20%7C00351> [hidden email] |00351 289244 495 >> ext: 7495; 7489; 7484 (mic lab) >> >> Dept. de Ciências Biomedicas e Medicina >> >> Uni. Algarve, Campus de Gambelas Ed 8, lab 1.18 >> >> 8005-139 Faro, Portugal. >> >> >> > |
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Claudia Florindo |
Re: Colibri vs HXP ... liquid light guide lifetime, recommended length, model, tips
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all Thanks a lot for your answers, It was very helpful Claudia. Claudia Florindo, PhD, Microscopy Unit Manager [hidden email] |00351 289244 495 ext: 7495; 7489; 7484 (mic lab) Dept. de Ciências Biomedicas e Medicina Uni. Algarve, Campus de Gambelas Ed 8, lab 1.18 8005-139 Faro, Portugal. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara Sent: domingo, 10 de Março de 2013 17:19 To: [hidden email] Subject: Re: Colibri vs HXP ... liquid light guide lifetime, recommended length, model, tips ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** At ABRF 2013, the Lumen Dynamics manager (Mic C.) told me that the liquid light guides have about a 3 year shelf life (all the LLG's are from one manufacturer: Lumatec). That is, they will "go bad" even in a drawer. So, congratulations on getting five years (though you may have a bubble without knowing it). His tips included: * when storing, humid environment is much better than dry (it is liquid inside). * kinks are bad. * if the output (aimed at a white, uniform surface) is not a clean, completely uniform disk, you have a bubble (or the LLG into the lamp is not in correctly). I have recommendations for labs and suppliers: * a right angle turn adapter for the LLG to MICROSCOPE ... a lot of microscopes have a back wall very close - current "bend the LLG" is a good way to damage it. * "label" the LLG with tape (or paint?) to make it obvious that this is not a power cord. * if the microscope configuration allows, use a ONE METER LLG instead of the more common 2 meter. * Lumatec manufactures several modesl, with different transmission spectra. Last time I checked (a couple of years ago), the LLG-380 (1 meter) was the most sensible choice. *** As for the Colibri vs HXP ... The Zeiss Colibri was expensive when it was introduced in 2007. Zeiss is likely to lower prices only if their competitors are successful selling at lower prices. If you want a Colibri, find out if they offer new (brighter, more available colors) LED's7: the LED field has progressed a lot since 2007. HXP: we are in 2013: don't waste your money on metal halide, Hg, or Xe lamps when "solid state" lighting is now available. In Houston, I will have a Lumencor SOLA on "my" Leica AM6000 microscope (DMI 6000 stand with 2 Eppendork micromanipulators). If I have an opportunity to upgrade soon, it would probably be to the Lumencor Spectra X (6 or 7 independent wavelengths, though pricey ... I have access to an almost identical product, InsightSSI, on the GE/API OMX upstairs for deconvolution and interference reflection contrast microscopy) or Lumen's 4 wavelength X-LED product (and possibly by an alternative LED or two for special needs ... of course if someone wants to donate some money to the lab, I'll be happy to put two X-LED's (8 wavelengths) on the AM6000 scope. Sincerely, George On 3/4/2013 12:07 PM, Julio Vazquez wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > One more thing to mention about metal halide: light is typically delivered via a light guide, which may cost around $ 500. Sometimes they go bad and need to be replaced, so this is also a factor to consider. Our light guides typically last a good five years if we are careful (they don't like to be bent, and they don't like to get hot). > -- > Julio Vazquez > Fred Hutchinson Cancer Research Center Seattle, USA > > http://www.fhcrc.org/ > > > > On Mar 1, 2013, at 8:05 AM, Claudia Florindo wrote: > > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> >> >> Dear all >> >> >> >> I was considering buying a zeiss colibri system instead of a HXP >> system, any comments in comparing both systems? >> >> Any suggestion would be very helpful, >> >> >> >> Thanks a lot, >> >> >> >> Claudia. >> >> >> >> >> >> Claudia Florindo, PhD, Microscopy Unit Manager >> >> <mailto:[hidden email]%20%7C00351> [hidden email] |00351 >> 289244 495 >> ext: 7495; 7489; 7484 (mic lab) >> >> Dept. de Ciências Biomedicas e Medicina >> >> Uni. Algarve, Campus de Gambelas Ed 8, lab 1.18 >> >> 8005-139 Faro, Portugal. >> >> >> > |
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Arvydas Matiukas |
Re: Colibri vs HXP ... liquid light guide lifetime, recommended length, model, tips
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In reply to this post by George McNamara
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** George, My experience is that LLG lifetime is directly related to their irradiation time/energy ( but not to their shelf time). I have two microscopy systems with LLGs that have been fine for 9 years. However, they have been used about 500hrs/year therefore their age in terms of usage 24/7 is only about 2 years. I do not see why LLGs should degrade staying in the drawer unless their solid material is not stable which again is suprising if it is amorphous ( or maybe all-penetrating cosmic rays degrade it?) Best, Arvydas >>> George McNamara <[hidden email]> 3/10/2013 12:19 PM >>> ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** At ABRF 2013, the Lumen Dynamics manager (Mic C.) told me that the liquid light guides have about a 3 year shelf life (all the LLG's are from one manufacturer: Lumatec). That is, they will "go bad" even in a drawer. So, congratulations on getting five years (though you may have a bubble without knowing it). His tips included: * when storing, humid environment is much better than dry (it is liquid inside). * kinks are bad. * if the output (aimed at a white, uniform surface) is not a clean, completely uniform disk, you have a bubble (or the LLG into the lamp is not in correctly). I have recommendations for labs and suppliers: * a right angle turn adapter for the LLG to MICROSCOPE ... a lot of microscopes have a back wall very close - current "bend the LLG" is a good way to damage it. * "label" the LLG with tape (or paint?) to make it obvious that this is not a power cord. * if the microscope configuration allows, use a ONE METER LLG instead of the more common 2 meter. * Lumatec manufactures several modesl, with different transmission spectra. Last time I checked (a couple of years ago), the LLG-380 (1 meter) was the most sensible choice. *** As for the Colibri vs HXP ... The Zeiss Colibri was expensive when it was introduced in 2007. Zeiss is likely to lower prices only if their competitors are successful selling at lower prices. If you want a Colibri, find out if they offer new (brighter, more available colors) LED's7: the LED field has progressed a lot since 2007. HXP: we are in 2013: don't waste your money on metal halide, Hg, or Xe lamps when "solid state" lighting is now available. In Houston, I will have a Lumencor SOLA on "my" Leica AM6000 microscope (DMI 6000 stand with 2 Eppendork micromanipulators). If I have an opportunity to upgrade soon, it would probably be to the Lumencor Spectra X (6 or 7 independent wavelengths, though pricey ... I have access to an almost identical product, InsightSSI, on the GE/API OMX upstairs for deconvolution and interference reflection contrast microscopy) or Lumen's 4 wavelength X-LED product (and possibly by an alternative LED or two for special needs ... of course if someone wants to donate some money to the lab, I'll be happy to put two X-LED's (8 wavelengths) on the AM6000 scope. Sincerely, George On 3/4/2013 12:07 PM, Julio Vazquez wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > One more thing to mention about metal halide: light is typically delivered via a light guide, which may cost around $ 500. Sometimes they go bad and need to be replaced, so this is also a factor to consider. Our light guides typically last a good five years if we are careful (they don't like to be bent, and they don't like to get hot). > -- > Julio Vazquez > Fred Hutchinson Cancer Research Center > Seattle, USA > > http://www.fhcrc.org/ > > > > On Mar 1, 2013, at 8:05 AM, Claudia Florindo wrote: > > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> >> >> Dear all >> >> >> >> I was considering buying a zeiss colibri system instead of a HXP >> comments in comparing both systems? >> >> Any suggestion would be very helpful, >> >> >> >> Thanks a lot, >> >> >> >> Claudia. >> >> >> >> >> >> Claudia Florindo, PhD, Microscopy Unit Manager >> >> <mailto:[hidden email]%20%7C00351> [hidden email] |00351 >> ext: 7495; 7489; 7484 (mic lab) >> >> Dept. de Ciências Biomedicas e Medicina >> >> Uni. Algarve, Campus de Gambelas Ed 8, lab 1.18 >> >> 8005-139 Faro, Portugal. >> >> >> > |
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