Commercial slide for spectral unmixing training

[G. Esteban Fernandez]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hello all,

I'd like to purchase a prepared slide containing spectrally
overlapping fluorophores to train users on unmixing.  Ideally it would
contain cells or tissue stained with defined fluorophores, not
inherent tissue fluorescence/"autofluorescence").  Does anyone know of
a commercial source for a slide like that?  (If not I can bum some
cultured cells, buy reagents, and prepare slides myself.)

Thanks,
Esteban

[Jürgen Schmied]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Esteban,

we could offer you custom nanostructures, based on DNA-origami, with a
defined number of dye-molecules, at defined distances and we could also
prepare samples with several different types of dye-molecules. You would
get a slide with millions of identical structures of known geometry.

Please have a look at our website: www.gattaquant.com
or contact us directly: [hidden email]

Best regards,
Jürgen Schmied
(CEO - GATTAquant)

2016-01-13 19:50 GMT+01:00 G. Esteban Fernandez <
[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello all,
>
> I'd like to purchase a prepared slide containing spectrally
> overlapping fluorophores to train users on unmixing.  Ideally it would
> contain cells or tissue stained with defined fluorophores, not
> inherent tissue fluorescence/"autofluorescence").  Does anyone know of
> a commercial source for a slide like that?  (If not I can bum some
> cultured cells, buy reagents, and prepare slides myself.)
>
> Thanks,
> Esteban
>

[Craig Brideau]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I find a combination of YFP and GFP is fun to unmix. They are fairly close
together and readily available in many forms.

Craig

On Wed, Jan 13, 2016 at 12:19 PM, Jürgen Schmied <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Esteban,
>
> we could offer you custom nanostructures, based on DNA-origami, with a
> defined number of dye-molecules, at defined distances and we could also
> prepare samples with several different types of dye-molecules. You would
> get a slide with millions of identical structures of known geometry.
>
> Please have a look at our website: www.gattaquant.com
> or contact us directly: [hidden email]
>
> Best regards,
> Jürgen Schmied
> (CEO - GATTAquant)
>
> 2016-01-13 19:50 GMT+01:00 G. Esteban Fernandez <
> [hidden email]>:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hello all,
> >
> > I'd like to purchase a prepared slide containing spectrally
> > overlapping fluorophores to train users on unmixing.  Ideally it would
> > contain cells or tissue stained with defined fluorophores, not
> > inherent tissue fluorescence/"autofluorescence").  Does anyone know of
> > a commercial source for a slide like that?  (If not I can bum some
> > cultured cells, buy reagents, and prepare slides myself.)
> >
> > Thanks,
> > Esteban
> >
>

[George McNamara]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Esteban,

Colorful Cell plasmid, $65

ColorfulCell <https://www.addgene.org/62449/>(Plasmid #62449)
PurposeMXS_chaining vector with 6 cassettes
Insert:*CMV::GTS-Citrine-bGHpA*Synthetic
Insert:*CMV::Lyn-Ceruleanx3-bGHpA*Synthetic
Insert:*CMV::NLS-TagBFPx3-bGHpA*Synthetic
Insert:*CMV::MTS-AzamiGreen-bGHpA*Synthetic
Insert:*CMV::iRFP670-SKL-bGHpA*Synthetic
Insert:*CMV::ETS-mCherry-KDEL-bGHpA*Synthetic
Tags: None
Expression:
Mutation: None
Use: Synthetic Biology
Deposited by:Pierre Neveu <https://www.addgene.org/Pierre_Neveu/>


*MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow
Cytometry Approaches in Mammalian Systems*. Sladitschek HL, Neveu
PA./PLoS One. 2015 Apr 24;10(4):e0124958. doi:
10.1371/journal.pone.0124958. eCollection 2015./PONE-D-15-01385
[pii]<https://www.addgene.org/browse/article/10274/>PubMed 25909630
<http://pubmed.org/25909630>

See also ES-Fucci
https://www.addgene.org/62451/

and

  * ChromosomesMembranesandCytoskeleton

https://www.addgene.org/62450/
(4plex)

Each $65. MXS kit is not that expensive (login to addgene for that price).

or ask the authors to mail you a slide(s). Maybe they can find cells
they transfected with each, get them all onto one coverglass (or imaging
dish), let grow for say 40 hours (see panel C of
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012539/figure/F4/ for
single HeLa cells growing into four cell 'tetrads" in 40 hours - credit
to Dr. Xiaoyu Jiang for this method, fits great on 63x/1.4NA oil
immersion objective lens, 2x confocal zoom), and send you that. If they
are up for making some slides, please let me know so I can ask for one
(or more).


Enjoy,
George


On 1/13/2016 12:50 PM, G. Esteban Fernandez wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello all,
>
> I'd like to purchase a prepared slide containing spectrally
> overlapping fluorophores to train users on unmixing.  Ideally it would
> contain cells or tissue stained with defined fluorophores, not
> inherent tissue fluorescence/"autofluorescence").  Does anyone know of
> a commercial source for a slide like that?  (If not I can bum some
> cultured cells, buy reagents, and prepare slides myself.)
>
> Thanks,
> Esteban
>

--



George McNamara, Ph.D.
Single Cells Analyst, T-Cell Therapy Lab (Cooper Lab)
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42
http://works.bepress.com/gmcnamara/75
https://www.linkedin.com/in/georgemcnamara