Shanna Banman |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello all, Does anyone know if there is a setting in Zen2009 for Zeiss LSM 710 that compensates for loss of fluorescence signal detection as you penetrate deeper into the sample? i.e. as you image deeper (100 um), the system automatically increases the laser intensity? Thank you!! Shanna Banman |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Yes. There is an advanced setting in the Z Series window at the bottom which controls this. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Shanna Banman Sent: Tuesday, September 04, 2012 6:57 PM To: [hidden email] Subject: Compensation for fluorescence loss Zeiss LSM 710 2-photon ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello all, Does anyone know if there is a setting in Zen2009 for Zeiss LSM 710 that compensates for loss of fluorescence signal detection as you penetrate deeper into the sample? i.e. as you image deeper (100 um), the system automatically increases the laser intensity? Thank you!! Shanna Banman |
Pascal Weber |
In reply to this post by Shanna Banman
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Yes sure you have two way to do that increase the power or/and pmt gain. It automatically interpolate power between two definite point. You cab save the curve and recall it when you want. |
Jacqueline Ross |
In reply to this post by Shanna Banman
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Shanna, Yes, as Michael mentioned already, you can do a correction with ZEN 2009. It's called Auto Z Brightness correction and you can find it in the Z stack dialogue window at the bottom. It's linear and is very easy to set up. Instructions are given on page 4-71 of the full manual that I have. I have a copy in PDF so if you don't have this, let me know and I will send it to your email address. Kind regards, Jacqui Jacqueline Ross Biomedical Imaging Microscopist Biomedical Imaging Research UnitĀ School of Medical SciencesĀ Faculty of Medical & Health Sciences The University of Auckland Private Bag 92019 Auckland 1142, NEW ZEALAND Tel: 64 9 923 7438 Fax: 64 9 373 7484 http://www.fmhs.auckland.ac.nz/sms/biru/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Shanna Banman Sent: Wednesday, 5 September 2012 10:57 a.m. To: [hidden email] Subject: Compensation for fluorescence loss Zeiss LSM 710 2-photon ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello all, Does anyone know if there is a setting in Zen2009 for Zeiss LSM 710 that compensates for loss of fluorescence signal detection as you penetrate deeper into the sample? i.e. as you image deeper (100 um), the system automatically increases the laser intensity? Thank you!! Shanna Banman |
Shanna Banman |
In reply to this post by Shanna Banman
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Thank you everyone for your help!! I really appreciate it and I will try it out! Cheers Shanna |
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