Compensation for fluorescence loss Zeiss LSM 710 2-photon

[Shanna Banman]

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Hello all,

Does anyone know if there is a setting in Zen2009 for Zeiss LSM 710 that
compensates for loss of fluorescence signal detection as you penetrate
deeper into the sample?  i.e. as you image deeper (100 um), the system
automatically increases the laser intensity?
Thank you!!

Shanna Banman

[mcammer]

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Yes.  There is an advanced setting in the Z Series window at the bottom which controls this.


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Shanna Banman
Sent: Tuesday, September 04, 2012 6:57 PM
To: [hidden email]
Subject: Compensation for fluorescence loss Zeiss LSM 710 2-photon

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Hello all,

Does anyone know if there is a setting in Zen2009 for Zeiss LSM 710 that compensates for loss of fluorescence signal detection as you penetrate deeper into the sample?  i.e. as you image deeper (100 um), the system automatically increases the laser intensity?
Thank you!!

Shanna Banman

[Pascal Weber]

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Yes sure you have two way to do that increase the power or/and pmt gain.
It automatically interpolate power between two definite point.
You cab save the curve and recall it when you want.

[Jacqueline Ross]

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Hi Shanna,

Yes, as Michael mentioned already, you can do a correction with ZEN 2009.

It's called Auto Z Brightness correction and you can find it in the Z stack dialogue window at the bottom. It's linear and is very easy to set up. Instructions are given on page 4-71 of the full manual that I have. I have a copy in PDF so if you don't have this, let me know and I will send it to your email address.

Kind regards,

Jacqui


Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Shanna Banman
Sent: Wednesday, 5 September 2012 10:57 a.m.
To: [hidden email]
Subject: Compensation for fluorescence loss Zeiss LSM 710 2-photon

*****
To join, leave or search the confocal microscopy listserv, go to:
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Hello all,

Does anyone know if there is a setting in Zen2009 for Zeiss LSM 710 that compensates for loss of fluorescence signal detection as you penetrate deeper into the sample?  i.e. as you image deeper (100 um), the system automatically increases the laser intensity?
Thank you!!

Shanna Banman

[Shanna Banman]

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Thank you everyone for your help!! I really appreciate it and I will try it out!

Cheers

Shanna