Confocal and Widefield microscopy training material
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Carol Heckman |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** At Bowling Green State University, we offer a graduate-level course, Light Microscopy and 3D Imaging. It trains the students to do most kinds of LM including confocal as well as computer interpretation of images. Carol Heckman ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Craig Brideau <[hidden email]> Sent: Thursday, October 17, 2019 3:46 PM To: [hidden email] <[hidden email]> Subject: [EXTERNAL] Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Here at University of Calgary we offer a Basic and an Advanced microscopy course, each taking a full semester. The Advanced class covers confocal and related techniques. We have had very good outcomes with graduate students who have completed these courses. Craig On Thu, Oct 17, 2019 at 1:34 PM Phillips, Thomas E. <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Does anybody offer their confocal training as an official grad-level > class? In the past, offering a hands-on class was problematic for us since > (i) no tuition dollars flow back to the core and (ii) PI's wanted their > students using their own samples so "free class time" meant no income for > us when those projects would normally have been done outside of class. Our > campus is moving to a new budget model and tuition dollars may flow more > directly back to us. We are considering combining training and supervised > independent use as part of the class design and allow student efforts to be > credentialed. I would be interested to know if anyone else is taking this > approach and how many credit hours you offer and what you cover as part of > the course. > > I have never met a PI who would have paid for Sylvie's design and > impressed her clients do so. > > > > Thomas E. Phillips, Ph.D > Professor of Biological Sciences > Curators’ Distinguished Teaching Professor > Director, Molecular Cytology Core > 2 Tucker Hall > University of Missouri > Columbia, MO 65211-7400 > 573-882-4712 (office) > 573-882-0123 (fax) > [hidden email] > > http://www.biology.missouri.edu/faculty/phillips.html > http://microscopy.missouri.edu > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> On > Behalf Of Kumar, Niyanta > Sent: Thursday, October 17, 2019 2:12 PM > To: [hidden email] > Subject: Re: Confocal and Widefield microscopy training material > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and > excellent training resources to go through! Much appreciated 😊 > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> On > Behalf Of Steffen Dietzel > Sent: Thursday, October 17, 2019 6:55 AM > To: [hidden email] > Subject: Re: Confocal and Widefield microscopy training material > > EXTERNAL EMAIL – Use caution with any links or file attachments. > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Niyanta, > > I have collected a few resources on our website, so that I can refer our > users to it more easily. This is at > https://www.bioimaging.bmc.med.uni-muenchen.de/learn/ > Have a look at "Educational Websites" and "Materials for teaching". > Maybe you find something helpful. The listed page from Australia (was > mentioned by others too) contains a quiz that you can take, and you can > print out a certificate if you answer 8+ of 10 questions correct. > > As in terms of hands on training, we start with a 3 h introduction session > for up to 3 people. Usually with our samples, for two reasons. > First, we want the user to pay attention to the microscope and software, > not on the biological content of the images we make. Second, this way we > can be sure that the sample is all right and we don't have users with > crappy samples blaming it on the microscope. I agree with others that after > 3 hours it makes no more sense, brains are full. > > Next we do a 'guided session' with the user's sample that we accompany > until the user is getting good results. This is 1:1. Depending on sample > difficulty, prior knowledge and user talent we sometimes have a third > session. > > I am impressed by Sylvie's approach, but I doubt our group leaders would > be willing to come up with so much money for training. Some think what we > do now is already too time consuming (but most are happy about it). > > > Steffen > > Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hi all, > > Can someone suggest any existing resources and formats to pull from > > for designing new and advanced user trainings for confocal and > > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > > resources or existing quizzes that can ensure users know what they > > need to would also be helpful. > > Thanks, > > Niyanta > > > -- > ------------------------------------------------------------ > Steffen Dietzel, PD Dr. rer. nat > Ludwig-Maximilians-Universität München > Biomedical Center (BMC) > Head of the Core Facility Bioimaging > > Großhaderner Straße 9 > D-82152 Planegg-Martinsried > Germany > > http://www.bioimaging.bmc.med.uni-muenchen.de > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New > Jersey, USA 07033), and/or its affiliates Direct contact information for > affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, please > notify us immediately by reply e-mail and then delete it from your system. > > |
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Straatman, Kees (Dr.) |
Re: Confocal and Widefield microscopy training material
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Sylvie Le Guyader |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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In reply to this post by Arvydas Matiukas
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear listers A long answer but, sorry, I got a bit agitated. How did such an enormous distortion of what I wrote happen? I never said that we train people for one month! 1h discussion + 5x3h training is what we do. How did this one-month thing come up? And during the 5x3h, the user has ample time to practice what we just taught her/him before we add one more layer. A lot of facility staff agree that 3h is totally not enough, many do 2-3 sessions of 3h, we add 2 more sessions and tons of value. And then I do not suppose that you start with sitting at the microscope and showing how to set the gain without having any idea what the sample/experiment will be, or do you? Well that part comes first and last 1h. Nothing outrageous! And this is no advance training. This is our basic. I do believe that at the end of a confocal training the user should know how each setting on the confocal can influence their results and they should have enough knowledge to make decisions on their own. If you buy a confocal, you get trained by the company and they explain things like pixel size, resolution, saturation, averaging, z stack, multidimension... That lasts longer than 3h. At least all the trainings I got from vendors did and I would not accept to get trained in 3h for a complex machine that costs several hundreds of thousands of dollars, would you? Why would a facility business model aim at delivering a worse training than companies who have no knowledge about/daily contact with samples and research as we facility people do? This totally doesn’t make sense to me. Our business model is that we educate our users so that they can redesign their experiments when their research requires without asking us which new settings they should use. But they anyway often ask for us to confirm their settings when they do that, because they know that, even if they have been using the facility for years and are very experienced, we can come up with great ideas and suggestions that will help them a lot. And we do this for free. They can get help for free from us any time after their training. If they go on parental leave for 1 year and come back, they get a free refresher (there go another 3h!). I believe that we facility people must present ourselves to researchers and group leaders as experts in microscopy experiment design. Because this is what we facility people are. We pile up the knowledge from tons of experiments and samples. We are a gold mine for researchers! During the first hour of discussion (for free) with our future users (and often their PIs), we do several things: * troubleshoot their sample prep and experimental design * open their eyes to the limits of what our equipment can deliver but also explain how much more they can do with our equipment compared to what they had initially planned * very importantly: we really brainstorm their experiment and team up with them during that time. When we are done, they leave with lots of free tailor-made information. They could then decide to go to another facility to work if they wanted. They have not paid anything yet. Instead, when they go, they always thank us greatly for our time, look at each other talking about all the details they need to change and realize that if they collaborate with us (this is the way I see our role as a facility, even if we are not on papers), their project will take a huge leap forward. They will gain tons of time and get meaningful data. Which group leader would NOT want to pay 1200 euros to save months of salaries and gain the assurance that their data is trustworthy? Why design a training to leave the trainee knowing how to push a few buttons but having to reinvent the wheel (and likely forgetting the spokes) about everything else that microscopy is? What I shared with you in my previous post (workflow of how to set a confocal) is not the workflow of our training, it is only the conclusion of the button-pushing part. This is a summary that is freely available on our website (under teaching material) and that we go through with them at the end to make sure they are very confident with answering all these questions for their sample/experiment. To me these points represent a minimum requirement, not an advanced training. These questions are not even half of the content of our basic training. Most of what we talk about is sample prep, experimental design and image analysis strategies. Not surprisingly to me, no one ever complains about the price and time. Btw, we also have a yearly microscopy course for PhD students and postdocs. The full schedule for 2019 is on our website and the 2020 will be up soon. All lectures are broadcasted live for free without registration. Varsågod as one says in Swedish. 😊 We also welcome people from other facilities who want to shadow us (for free) or be trained by us (for the same fee as everyone). This year we trained people from Poland and Turkey that way. We are a university. Our role is to deliver the best possible education and research to society. To help everyone compare to their own facility, here are a few numbers about us: - we have 9 advanced microscopes with all sorts of functions (11 year-old facility, Nikon Center of Excellence since 2014) - about 90 users from 50 different groups (and shades of samples) - we are 2.8 persons (director 10%, manager 100%, research engineer 100%, research engineer 50%, computer scientist 20%) - our training (which we call Entry) and Usage fees cover 37% of our total budget including all salaries, maintenance and upgrading machines, only excluding purchasing new machines for which we need to get dedicated grants. - We also have an unusual fee model: our users pay one Usage fee per year (or 6, 3 or 1 months) which currently costs 18000 euros per year and there is no additional hourly fee. All booking rules and such are found on our website/using the facility/guidelines for those who want to know more. Less boring admin, more time for trainings and fun collaborations! 😃 And unlike Kees, we never acquire images for anyone. If people do not have time to get trained, they collaborate with one of our users. And we have the advantage that our systems are all in the same room so we are probably less trained that he is! All comments about us are very welcome. I especially welcome comments about any mistake you find on our website/training material/during our course. I strongly believe in lifetime learning. 😊 Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Arvydas Matiukas Sent: 17 October 2019 20:37 To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C189366ab217145c341dc08d75332bac7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637069349657725877&sdata=Uw495XDm93PClu4a0Ge61sjaV9DIw3Bqw5wqdxZcyJ0%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C189366ab217145c341dc08d75332bac7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637069349657725877&sdata=dpL2GOaVTKVxvFO7y%2F93U%2FGDxNcZyIQT%2BUPlMMR3V9Y%3D&reserved=0 and include the link in your posting. ***** Hi Steffen, Silvie Thanks for very interesting and useful discussion. Few summary thoughts: 1. I second Steffens' reasoning for starting training with Core provided=known=calibrated samples. Sylvie's approach of starting with users'=non calibrated sample introduces to many unknowns at a time and to my opinion reduces efficiency of the training. I personally prefer not to be trained in such stressful way. 2. I wonder if many labs in EU can afford to spend 1 month of their member time and pay 1200EUR to get the members trained in advanced level microscopy. In the USA it definitely not going to happen on any significant scale. Maybe a cultural difference :) I recall the comparison provided by US based Zeiss specialist after his training in Germany: "problem= ballpoint pen stopped writing. German style solution = research potential causes, i.e. disassemble the pen, check if any ink is left, if ball rotates freely, finally if the user properly presses knob or rotates the body. This approach may take significant but reasonable time and provide useful skills and experience for the future. American style solution = check the pen price first. As it is much lower than the fee of professional spending hours on this problem then just trash the pen and get a new one" 3. Of course in the USA there are labs that need advanced level microscopy. However, typically they have their own microscopes and microscopy experts and very rarely use Core services. Among my 400 trainees over 15 years only about 10 expressed willingness to proceed to the advanced level, and among them only 1 grad student. 4. Finally, time and cost considerations. At 1 month and 1200EUR spending level how many techniques a beginner researcher may learn in 1 year ? 3years? BTW I think training in optical/confocal microscopy is relatively inexpensive, preparing your own samples typically is more time consuming and expensive, and learning other tecniques may be more expensive as well. My 2 cents, Arvydas +++++++++++++++++++++++++++++ Arvydas Matiukas, Ph.D. Manager of NRB Shared Equipment Director of Confocal&Two-Photon Core SUNY Upstate Medical University Neuroscience & Physiology Dept >>> Steffen Dietzel <[hidden email]<mailto:[hidden email]>> 10/17/2019 6:55 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIDaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3D99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0%26s%3Dk0lBHjfBdP6gkIAbpZx0fMuOqb1oDSvuwF7Hk7nelgY%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C189366ab217145c341dc08d75332bac7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637069349657725877&sdata=33%2B4%2FtGWwiqvsmKiNzGAW0aukHIA2%2FpAmrsZ9VBSjMM%3D&reserved=0= Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIDaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3D99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0%26s%3DyAGFeShn7KSa-eW2mPu6XbhS9SeX7BD0-L0UF5cyjgE%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C189366ab217145c341dc08d75332bac7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637069349657725877&sdata=ZZTTcrCt02Ajc2FRmgIqS%2B02bjk1fr%2FsdS2sD7XeCJk%3D&reserved=0= and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_learn_%26d%3DDwIDaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3D99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0%26s%3DZ2jynrNg35the3m1H5X3AsUA52lnDqOb4qZUaoY05dY%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C189366ab217145c341dc08d75332bac7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637069349657725877&sdata=wTlu4BQcx4umweBHRlkLTKN0GgUDShIwPDTI6%2F91GmU%3D&reserved=0= Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIDaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3D99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0%26s%3Dk0lBHjfBdP6gkIAbpZx0fMuOqb1oDSvuwF7Hk7nelgY%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C189366ab217145c341dc08d75332bac7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637069349657725877&sdata=33%2B4%2FtGWwiqvsmKiNzGAW0aukHIA2%2FpAmrsZ9VBSjMM%3D&reserved=0= > Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIDaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3D99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0%26s%3DyAGFeShn7KSa-eW2mPu6XbhS9SeX7BD0-L0UF5cyjgE%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C189366ab217145c341dc08d75332bac7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637069349657735871&sdata=JGUsYIzKk6PJ2T5jsCarT8P8Xn%2B4Dd7MqUtQMJqA%2B28%3D&reserved=0= and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität Mnchen Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de%26d%3DDwIDaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3D99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0%26s%3DGadulq06lK7kYY94plB3DsipjiGMo01xTiPc_poN3_Y%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C189366ab217145c341dc08d75332bac7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637069349657735871&sdata=AmakLWZCfe8xWPdce1PWi%2FuB53USTGlG3c612J1%2FNsM%3D&reserved=0= När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. 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Julian Smith III |
Re: Confocal and Widefield microscopy training material
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In reply to this post by Straatman, Kees (Dr.)
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Here (at a PUI), I teach a microscopy methods course that includes training on the confocal (mixed grad/undergrad, about 10 students every other year). Most of our undergrads doing research start in their second year (or even before), so I only have 1 or 2 (sometimes none) to train between course offerings. We have no internal recharges (and no technician), the Dean's office pays for service contracts on the confocal, SEM, and TEM, and the course has a budget of $1500. Beyond that, the facility gets a small amount of support from external-user recharges. I do help with protocol optimization once the students are out of the course and in someone's lab. We are experimenting with having external users hire one of our graduates as a part-time technician. Major equipment replacement is supported centrally, and shows up as a pulse of money ($300k-$400k) about every 5 years; it's often not enough for new (our FACS is new, our "new" TEM is ~10 years old; our Live-cell microscope system was built from used parts), but it does keep us from falling completely behind R1's and private Universities in terms of what we can offer our students and faculty. Best, Julian On 10/18/19 3:59 AM, Straatman, Kees (Dr.) wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Very interesting discussion. > > Here in Leicester I train users in 3.5 hour 1 to 1 sessions on the microscope they want to use and with their sample, so I can show them how to setup the microscope and optimize it for their use. If I am happy with their progress they get an account and the next time they want to use the microscope they have to make an appointment with me or bring an experienced user from their group, so we are sure they are able to start the system and get an image on the screen. After that it is up to the user. I also would not have the time/resources to spend more time on training each individual user. My facility is spread over 4 buildings so I am not always around to guide and solve problems, but I walk in and try to keep an eye on their progress. Occasionally I have a user that fails the 'progress test' and they will get a second induction session. If I am still not happy with their ability to use the microscope they have to make appointments with me to do their imaging. With some users I do the imaging or I keep a close eye on their imaging because their subject is difficult and there are some user who want me to do their imaging and they always can make an appointment with me to sit-in on one of their imaging sessions. If I do the imaging it is a collaboration and I would be co-author on any paper resulting from this work. This is charged on the standard rate of using a microscope for internal users, external users have to pay for my hours as well. > > Qua teaching the facility is involved in several courses. I think it is always good if the students know we are around as they are potential future users. None of the lectures bring in money, nor undergrad use of the microscopes during courses but postgraduate courses on the microscopes get charged for hourly use of the system. > > Best wishes > > Kees > > > Dr Ir K.R. Straatman > Senior Experimental Officer > Advanced Imaging Facility > Centre for Core Biotechnology Services > University of Leicester > www.le.ac.uk/advanced-imaging-facility > > ImageJ workshops 16 and 17 December https://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/AIF/workshops/imagej-workshops-December-2019 > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> On Behalf Of Cammer, Michael > Sent: 17 October 2019 21:38 > To: [hidden email] > Subject: Re: Confocal and Widefield microscopy training material > > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085664628&sdata=yCzLRzjhLR0ZzdfJeljgij7wmZkHggG1nToW3Tj9lqU%3D&reserved=0 > Post images on https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085674626&sdata=986DK6iTb53OsLFZFpqdaHsMA2dVQVFfu6anomWQgvc%3D&reserved=0 and include the link in your posting. > ***** > > I have pinch hit lectured and ran labs in many courses. Sometimes the instrument time was paid for out of an education budget, but mostly not. The core (or me personally since most lectures were prepared at home) absorbed the costs of my time. > > I also taught two years of a week-long course for new grad students. I sent bills, but don’t know if they were actually paid. > > Teaching does bring in business. Students who find our teaching useful or interesting spread the word. > > Cheers- > Michael Cammer > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> On Behalf Of Oshel, Philip Eugene > Sent: Thursday, October 17, 2019 3:47 PM > To: [hidden email] > Subject: Re: Confocal and Widefield microscopy training material > > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085674626&sdata=7iuQz3nyCVjKnEclvzI3WKoUieJ5rX3tjAOYbJDyKs0%3D&reserved=0= > Post images on https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085674626&sdata=WOYLkYM89g5dzOdvM%2F3q%2FzAKWj25wqx74ZYuYVQxH9k%3D&reserved=0= and include the link in your posting. > ***** > > We teach a grad-level microscopy course in confocal (also SEM & TEM courses). Tuition dollars don't come to the facility, but the instrument use (student use hours) is billed to the course, and that money does come to the facility. The instruments are billed at the standard "in-house" rate. > Note: This are the same courses the undergrads in our "microscopy major" take. > > Phil > ------------- > Philip Oshel > Imaging Facility Director > Biology Department > 1304 Biosciences > 1455 Calumet Ct. > Central Michigan University > Mt. Pleasant, MI 48859 > 989 774-3576 office > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> on behalf of "Phillips, Thomas E." <[hidden email]> > Reply-To: Confocal Microscopy List <[hidden email]> > Date: Thursday, 17October, 2019 at 15:34 > To: "[hidden email]" <[hidden email]> > Subject: Re: Confocal and Widefield microscopy training material > > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085674626&sdata=7iuQz3nyCVjKnEclvzI3WKoUieJ5rX3tjAOYbJDyKs0%3D&reserved=0= > Post images on https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085674626&sdata=WOYLkYM89g5dzOdvM%2F3q%2FzAKWj25wqx74ZYuYVQxH9k%3D&reserved=0= and include the link in your posting. > ***** > > Does anybody offer their confocal training as an official grad-level class? In the past, offering a hands-on class was problematic for us since (i) no tuition dollars flow back to the core and (ii) PI's wanted their students using their own samples so "free class time" meant no income for us when those projects would normally have been done outside of class. Our campus is moving to a new budget model and tuition dollars may flow more directly back to us. We are considering combining training and supervised independent use as part of the class design and allow student efforts to be credentialed. I would be interested to know if anyone else is taking this approach and how many credit hours you offer and what you cover as part of the course. > > I have never met a PI who would have paid for Sylvie's design and impressed her clients do so. > > > > Thomas E. Phillips, Ph.D > Professor of Biological Sciences > Curators’ Distinguished Teaching Professor > Director, Molecular Cytology Core > 2 Tucker Hall > University of Missouri > Columbia, MO 65211-7400 > 573-882-4712 (office) > 573-882-0123 (fax) > [hidden email] > > https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.biology.missouri.edu_faculty_phillips.html%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3D2X2JzDknmfdEUySiYs80Xwo3E8_H7XOtsRvsqjyk-as%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085674626&sdata=Cug%2FinADfapbKojiWUSCAeosEzCD1exdwotF4abnazQ%3D&reserved=0= > https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__microscopy.missouri.edu%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DnH6sgQk_G8l8jC3aTU7fVEdy8GAniz_FC3AxsckNPMs%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085674626&sdata=dM3mvateP03oE4xPM6YTQSIxvmbUhtSSR7bm6u1r4hM%3D&reserved=0= > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> On Behalf Of Kumar, Niyanta > Sent: Thursday, October 17, 2019 2:12 PM > To: [hidden email] > Subject: Re: Confocal and Widefield microscopy training material > > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085674626&sdata=7iuQz3nyCVjKnEclvzI3WKoUieJ5rX3tjAOYbJDyKs0%3D&reserved=0= > Post images on https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085684621&sdata=1%2FSXWCyJkeqhNdYepwNq6GGT8hnD%2BGR9K9UsFKDeFrE%3D&reserved=0= and include the link in your posting. > ***** > > Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊 > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel > Sent: Thursday, October 17, 2019 6:55 AM > To: [hidden email] > Subject: Re: Confocal and Widefield microscopy training material > > EXTERNAL EMAIL – Use caution with any links or file attachments. > > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085684621&sdata=2H1UIUilUFDXHkvYbROAbG47ZN%2FlpWZQ3IS%2BALNrQAw%3D&reserved=0= > Post images on https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085684621&sdata=1%2FSXWCyJkeqhNdYepwNq6GGT8hnD%2BGR9K9UsFKDeFrE%3D&reserved=0= and include the link in your posting. > ***** > > Hi Niyanta, > > I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_learn_%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3D8ICIFmvnIJoBPS2mAi7Ci-kXZCRIY5Bv8knrcEHDEtQ%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085684621&sdata=E%2F0OsUUINWk3uvAfzPOuZXNEWwaanBZAx15mHNr9BT4%3D&reserved=0= > Have a look at "Educational Websites" and "Materials for teaching". > Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. > > As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. > First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. > > Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. > > I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). > > > Steffen > > Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085684621&sdata=2H1UIUilUFDXHkvYbROAbG47ZN%2FlpWZQ3IS%2BALNrQAw%3D&reserved=0= > > Post images on https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085684621&sdata=1%2FSXWCyJkeqhNdYepwNq6GGT8hnD%2BGR9K9UsFKDeFrE%3D&reserved=0= and include the link in your posting. > > ***** > > > > Hi all, > > Can someone suggest any existing resources and formats to pull from > > for designing new and advanced user trainings for confocal and > > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > > resources or existing quizzes that can ensure users know what they > > need to would also be helpful. > > Thanks, > > Niyanta > > > -- > ------------------------------------------------------------ > Steffen Dietzel, PD Dr. rer. nat > Ludwig-Maximilians-Universität München > Biomedical Center (BMC) > Head of the Core Facility Bioimaging > > Großhaderner Straße 9 > D-82152 Planegg-Martinsried > Germany > > https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DXjRa4ovswLBxD4kAWsQfoP7jxzMCqpm5soZBwmqyrBQ%26e&data=02%7C01%7Ckrs5%40LEICESTER.AC.UK%7Cb3a6aaf7b844492d52a508d75341f576%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637069415085684621&sdata=yGG3jK6uPTIuZwY4aM7spGRmutZcKlIW2QHNvMOZQyA%3D&reserved=0= > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. 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Roberts,Owen S |
Re: Confocal and Widefield microscopy training material
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In reply to this post by Straatman, Kees (Dr.)
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** First a bit of an introduction as I am new to the list, but have consulted it for years. I am caretaker of a Olympus FV1000 with a mix of DPSS, Ion, and Diode lasers, with two AOTF filters on a BX61 Frame. I'm an electronics specialist who maintains the microscope, deals with the vendors, and trains the students. Our Confocal was purchased for corrosion research, but that took another direction. Having a background in Ion lasers, spectroscopy, high speed, and infrared imaging, my manager decided I should pick up the confocal when I joined the department. So while we consider the confocal a department instrument, most of our users are graduate biology and biomedical engineering students. Our policy is to graciously share, as the microscope is depreciated, having sat with very little use for a long time. This is an auxiliary duty for me, and runs "Time Available", so training occurs at a mutually agreeable time and day, so I can take care of my primary duties. I ask PIs to donate the main consumable, the arc lamps. The argon laser operates at a very low current, so the tube life will be very long, as I take care to enforce warm-up and shut down schedules to prolong tube life. Having looked at the costs and time involved to bill internally, its actually less cost for us to just leave it open, as it is not in use on a daily basis. We have several institutions near by that have instruments and charge a low rate for their use, so PIs have a choice of a modern, more sophisticated instrument at 30$ or so an hour, or the FV1000. When they have a good grant they use an instrument about 20 miles away, otherwise they use ours. So my trainees usually graduate to a more modern instrument at some time. Training consists of about six "one on one" or "small group" hours with me, with a break for lunch. It consists of some basic optics, hardware, and fluorescence theory, rigorously enforced procedures to protect the instrument, and imaging of some carefully constructed test and calibration slides using a variety of phosphors and polymers to simulate dyes. I keep a PMT tube around as a training aide as well. If a student has a sample prepared, they are welcome to bring it. One of my goals is to break the theory that some PIs have that a set of notebook based settings, handed down, will work with every sample. So we work on finding an image and optimizing it. I'll then schedule a session with each student and their own samples. If I'm convinced they are progressing, they get a "License to Drive", and an introduction to our scheduling calendar. I'll then gradually wean them off of having me present. They have my cell number and I'll check in at random to see how they are doing. I have great freedom over what I do, and I generally can get back to the microscopy lab in less then ten minutes if they are having an issue. If a student is using the wet objectives, or tiling, I'll do a another full day with them. Only two students in five years have needed me full time to do their imaging for them. My degree is in secondary education, so I use a scaffold learning process to teach students self confidence in using the instrument. As we are a College of Engineering, next to a College of Polymer Science, often I get requests to help with non-standard or new methodologies, and we push the machine to its limits some times. Quantum Dots, Corrosion Samples, Hydrogels, things that are out of the biological realm, get my full time attention. The AOTFs enable these areas of research. No tests, no quizzes, some optional pre-reading on their own time, and personalized help. I'm not a biologist, so if I get stuck, I reach out to those who might know. Steve Steve Roberts Senior Technician, Instrumentation The Department of Chemical and Bio-Molecular Engineering The University of Akron Cell: 330-245-7571 ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Straatman, Kees (Dr.) <[hidden email]> Sent: Friday, October 18, 2019 3:59 AM To: [hidden email] <[hidden email]> Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480685610&sdata=fDiDUgCc8%2B7k9etqe%2BUavYL7P3aUghcO8MNesRPMfmw%3D&reserved=0 Post images on https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480685610&sdata=8vZj6orkSg9oSqFeh5NquaS6RM1MtRpvdWzhQs1cA3w%3D&reserved=0 and include the link in your posting. ***** Very interesting discussion. Here in Leicester I train users in 3.5 hour 1 to 1 sessions on the microscope they want to use and with their sample, so I can show them how to setup the microscope and optimize it for their use. If I am happy with their progress they get an account and the next time they want to use the microscope they have to make an appointment with me or bring an experienced user from their group, so we are sure they are able to start the system and get an image on the screen. After that it is up to the user. I also would not have the time/resources to spend more time on training each individual user. My facility is spread over 4 buildings so I am not always around to guide and solve problems, but I walk in and try to keep an eye on their progress. Occasionally I have a user that fails the 'progress test' and they will get a second induction session. If I am still not happy with their ability to use the microscope they have to make appointments with me to do their imaging. With some users I do the imaging or I keep a close eye on their imaging because their subject is difficult and there are some user who want me to do their imaging and they always can make an appointment with me to sit-in on one of their imaging sessions. If I do the imaging it is a collaboration and I would be co-author on any paper resulting from this work. This is charged on the standard rate of using a microscope for internal users, external users have to pay for my hours as well. Qua teaching the facility is involved in several courses. I think it is always good if the students know we are around as they are potential future users. None of the lectures bring in money, nor undergrad use of the microscopes during courses but postgraduate courses on the microscopes get charged for hourly use of the system. Best wishes Kees Dr Ir K.R. Straatman Senior Experimental Officer Advanced Imaging Facility Centre for Core Biotechnology Services University of Leicester https://nam03.safelinks.protection.outlook.com/?url=www.le.ac.uk%2Fadvanced-imaging-facility&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480685610&sdata=61Eru3QA%2FHOt%2F%2BVKli6u5fwon0rcVq9UaMDZll6JUO4%3D&reserved=0 ImageJ workshops 16 and 17 December https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww2.le.ac.uk%2Fcolleges%2Fmedbiopsych%2Ffacilities-and-services%2Fcbs%2FAIF%2Fworkshops%2Fimagej-workshops-December-2019&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480685610&sdata=jlLP5LrKpzh4OJmtBpb3LnY8gpX1j28nNsDIIUkL0sE%3D&reserved=0 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Cammer, Michael Sent: 17 October 2019 21:38 To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480685610&sdata=fDiDUgCc8%2B7k9etqe%2BUavYL7P3aUghcO8MNesRPMfmw%3D&reserved=0 Post images on https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480685610&sdata=8vZj6orkSg9oSqFeh5NquaS6RM1MtRpvdWzhQs1cA3w%3D&reserved=0 and include the link in your posting. ***** I have pinch hit lectured and ran labs in many courses. Sometimes the instrument time was paid for out of an education budget, but mostly not. The core (or me personally since most lectures were prepared at home) absorbed the costs of my time. I also taught two years of a week-long course for new grad students. I sent bills, but don’t know if they were actually paid. Teaching does bring in business. Students who find our teaching useful or interesting spread the word. Cheers- Michael Cammer -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Oshel, Philip Eugene Sent: Thursday, October 17, 2019 3:47 PM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480685610&sdata=QUqOTjtOJcPD3yWf8gc5ElQHCDDhC1WUVXlsqthUx7E%3D&reserved=0= Post images on https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480695608&sdata=yK2j3eqNCD%2FKSR0Ja1vdNnjHJpuBYhCMlJTqcvFTLN4%3D&reserved=0= and include the link in your posting. ***** We teach a grad-level microscopy course in confocal (also SEM & TEM courses). Tuition dollars don't come to the facility, but the instrument use (student use hours) is billed to the course, and that money does come to the facility. The instruments are billed at the standard "in-house" rate. Note: This are the same courses the undergrads in our "microscopy major" take. Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office -----Original Message----- From: Confocal Microscopy List <[hidden email]> on behalf of "Phillips, Thomas E." <[hidden email]> Reply-To: Confocal Microscopy List <[hidden email]> Date: Thursday, 17October, 2019 at 15:34 To: "[hidden email]" <[hidden email]> Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480695608&sdata=t9YDHnbgdSARyeRuzf7BDG3MCeBRYFLw%2BlY%2B0yv8LEw%3D&reserved=0= Post images on https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480695608&sdata=yK2j3eqNCD%2FKSR0Ja1vdNnjHJpuBYhCMlJTqcvFTLN4%3D&reserved=0= and include the link in your posting. ***** Does anybody offer their confocal training as an official grad-level class? In the past, offering a hands-on class was problematic for us since (i) no tuition dollars flow back to the core and (ii) PI's wanted their students using their own samples so "free class time" meant no income for us when those projects would normally have been done outside of class. Our campus is moving to a new budget model and tuition dollars may flow more directly back to us. We are considering combining training and supervised independent use as part of the class design and allow student efforts to be credentialed. I would be interested to know if anyone else is taking this approach and how many credit hours you offer and what you cover as part of the course. I have never met a PI who would have paid for Sylvie's design and impressed her clients do so. Thomas E. Phillips, Ph.D Professor of Biological Sciences Curators’ Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email] https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.biology.missouri.edu_faculty_phillips.html%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3D2X2JzDknmfdEUySiYs80Xwo3E8_H7XOtsRvsqjyk-as%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480695608&sdata=ck63OutRO9Ft4sYzYgX4iqHy1Ka%2Bpib6A5EtMOYmnEY%3D&reserved=0= https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__microscopy.missouri.edu%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DnH6sgQk_G8l8jC3aTU7fVEdy8GAniz_FC3AxsckNPMs%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480695608&sdata=Q2NsEj1FA%2BrKMbnC9qyD%2Fq4%2F60i2NFSBWC98hUlE5DU%3D&reserved=0= -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Kumar, Niyanta Sent: Thursday, October 17, 2019 2:12 PM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480695608&sdata=t9YDHnbgdSARyeRuzf7BDG3MCeBRYFLw%2BlY%2B0yv8LEw%3D&reserved=0= Post images on https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480695608&sdata=yK2j3eqNCD%2FKSR0Ja1vdNnjHJpuBYhCMlJTqcvFTLN4%3D&reserved=0= and include the link in your posting. ***** Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel Sent: Thursday, October 17, 2019 6:55 AM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material EXTERNAL EMAIL – Use caution with any links or file attachments. ***** To join, leave or search the confocal microscopy listserv, go to: https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480705599&sdata=KpAhiiCSIMX9jYtVmGR4B00rqe%2FPINZXUXNXODkMz7Y%3D&reserved=0= Post images on https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480705599&sdata=ENQv8ZCo%2F40CBRZjSlvqQffWfl%2BVLXg8whlYTg1B13U%3D&reserved=0= and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_learn_%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3D8ICIFmvnIJoBPS2mAi7Ci-kXZCRIY5Bv8knrcEHDEtQ%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480705599&sdata=ZQe%2FdENRRKUC%2BQBAg8b7Oh%2BuBbVhC0pBZkPCkyw7obs%3D&reserved=0= Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480705599&sdata=KpAhiiCSIMX9jYtVmGR4B00rqe%2FPINZXUXNXODkMz7Y%3D&reserved=0= > Post images on https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480705599&sdata=ENQv8ZCo%2F40CBRZjSlvqQffWfl%2BVLXg8whlYTg1B13U%3D&reserved=0= and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany https://nam03.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DXjRa4ovswLBxD4kAWsQfoP7jxzMCqpm5soZBwmqyrBQ%26e&data=02%7C01%7Cosr%40UAKRON.EDU%7Ce3d626565ab74b3936b308d753a147ca%7Ce8575dedd7f94ecea4aa0b32991aeedd%7C0%7C0%7C637069824480705599&sdata=1MK9SDB95FckR2OdxxP6ip5cAfvwctVCRZ8l2GfjFXI%3D&reserved=0= Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. 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Arvydas Matiukas |
Re: Confocal and Widefield microscopy training material
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In reply to this post by Sylvie Le Guyader
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** The further the more deep and interesting the discussion gets! With the details provided I see that Silvie's business plan and microscopy education approach matches the university environment and policy. And I wish her the best luck spreading good quality microscopy education. However, not every Core is immersed in environment of that large scale and wisely organized . Remove undergrads, Excellence Center status, scale instrument # at 20%, student / lab # and finances/resources at 10% - you will get our environment in which we still need to survive adapting our policies . Regarding min training hrs required for the trainee to be able to run confocal without supervision. It also depends on the "user friendliness" of the software. Nikon software (that Silvie mentioned) is very flexible but not very user friendly while Zeiss is much more intuitive and 3 hrs may be enough (no commercial interest, just my personal opinion) . Best regards, Arvydas Click to add a signature ( gwsig:addsig) >>> Sylvie Le Guyader <[hidden email]> 10/18/2019 4:42 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=SGQ43Lb3oE49o-xeu2RvxrdKC8nNB2LdqthBVQYxcSc&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=a4uHDqqZ1djwdyonkSuqCBiEBwBX6vXQoi6fFvwKnaE&e= and include the link in your posting. ***** Dear listers A long answer but, sorry, I got a bit agitated. How did such an enormous distortion of what I wrote happen? I never said that we train people for one month! 1h discussion + 5x3h training is what we do. How did this one-month thing come up? And during the 5x3h, the user has ample time to practice what we just taught her/him before we add one more layer. A lot of facility staff agree that 3h is totally not enough, many do 2-3 sessions of 3h, we add 2 more sessions and tons of value. And then I do not suppose that you start with sitting at the microscope and showing how to set the gain without having any idea what the sample/experiment will be, or do you? Well that part comes first and last 1h. Nothing outrageous! And this is no advance training. This is our basic. I do believe that at the end of a confocal training the user should know how each setting on the confocal can influence their results and they should have enough knowledge to make decisions on their own. If you buy a confocal, you get trained by the company and they explain things like pixel size, resolution, saturation, averaging, z stack, multidimension... That lasts longer than 3h. At least all the trainings I got from vendors did and I would not accept to get trained in 3h for a complex machine that costs several hundreds of thousands of dollars, would you? Why would a facility business model aim at delivering a worse training than companies who have no knowledge about/daily contact with samples and research as we facility people do? This totally doesn’t make sense to me. Our business model is that we educate our users so that they can redesign their experiments when their research requires without asking us which new settings they should use. But they anyway often ask for us to confirm their settings when they do that, because they know that, even if they have been using the facility for years and are very experienced, we can come up with great ideas and suggestions that will help them a lot. And we do this for free. They can get help for free from us any time after their training. If they go on parental leave for 1 year and come back, they get a free refresher (there go another 3h!). I believe that we facility people must present ourselves to researchers and group leaders as experts in microscopy experiment design. Because this is what we facility people are. We pile up the knowledge from tons of experiments and samples. We are a gold mine for researchers! During the first hour of discussion (for free) with our future users (and often their PIs), we do several things: * troubleshoot their sample prep and experimental design * open their eyes to the limits of what our equipment can deliver but also explain how much more they can do with our equipment compared to what they had initially planned * very importantly: we really brainstorm their experiment and team up with them during that time. When we are done, they leave with lots of free tailor-made information. They could then decide to go to another facility to work if they wanted. They have not paid anything yet. Instead, when they go, they always thank us greatly for our time, look at each other talking about all the details they need to change and realize that if they collaborate with us (this is the way I see our role as a facility, even if we are not on papers), their project will take a huge leap forward. They will gain tons of time and get meaningful data. Which group leader would NOT want to pay 1200 euros to save months of salaries and gain the assurance that their data is trustworthy? Why design a training to leave the trainee knowing how to push a few buttons but having to reinvent the wheel (and likely forgetting the spokes) about everything else that microscopy is? What I shared with you in my previous post (workflow of how to set a confocal) is not the workflow of our training, it is only the conclusion of the button-pushing part. This is a summary that is freely available on our website (under teaching material) and that we go through with them at the end to make sure they are very confident with answering all these questions for their sample/experiment. To me these points represent a minimum requirement, not an advanced training. These questions are not even half of the content of our basic training. Most of what we talk about is sample prep, experimental design and image analysis strategies. Not surprisingly to me, no one ever complains about the price and time. Btw, we also have a yearly microscopy course for PhD students and postdocs. The full schedule for 2019 is on our website and the 2020 will be up soon. All lectures are broadcasted live for free without registration. Varsågod as one says in Swedish. �� We also welcome people from other facilities who want to shadow us (for free) or be trained by us (for the same fee as everyone). This year we trained people from Poland and Turkey that way. We are a university. Our role is to deliver the best possible education and research to society. To help everyone compare to their own facility, here are a few numbers about us: - we have 9 advanced microscopes with all sorts of functions (11 year-old facility, Nikon Center of Excellence since 2014) - about 90 users from 50 different groups (and shades of samples) - we are 2.8 persons (director 10%, manager 100%, research engineer 100%, research engineer 50%, computer scientist 20%) - our training (which we call Entry) and Usage fees cover 37% of our total budget including all salaries, maintenance and upgrading machines, only excluding purchasing new machines for which we need to get dedicated grants. - We also have an unusual fee model: our users pay one Usage fee per year (or 6, 3 or 1 months) which currently costs 18000 euros per year and there is no additional hourly fee. All booking rules and such are found on our website/using the facility/guidelines for those who want to know more. Less boring admin, more time for trainings and fun collaborations! �� And unlike Kees, we never acquire images for anyone. If people do not have time to get trained, they collaborate with one of our users. And we have the advantage that our systems are all in the same room so we are probably less trained that he is! All comments about us are very welcome. I especially welcome comments about any mistake you find on our website/training material/during our course. I strongly believe in lifetime learning. �� Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Arvydas Matiukas Sent: 17 October 2019 20:37 To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Flists.umn.edu-252Fcgi-2Dbin-252Fwa-253FA0-253Dconfocalmicroscopy-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3DUw495XDm93PClu4a0Ge61sjaV9DIw3Bqw5wqdxZcyJ0-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=qjVl4iRRCGRDTthYRfxqZeAAoo93FWJhGf9jz50jlt0&e= Post images on https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Fwww.imgur.com-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3DdpL2GOaVTKVxvFO7y-252F93U-252FGDxNcZyIQT-252BUPlMMR3V9Y-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=XMwNbMdDPanoMyqohSt6QY7IAzBiIhAon5c5Fn6K5lI&e= and include the link in your posting. ***** Hi Steffen, Silvie Thanks for very interesting and useful discussion. Few summary thoughts: 1. I second Steffens' reasoning for starting training with Core provided=known=calibrated samples. Sylvie's approach of starting with users'=non calibrated sample introduces to many unknowns at a time and to my opinion reduces efficiency of the training. I personally prefer not to be trained in such stressful way. 2. I wonder if many labs in EU can afford to spend 1 month of their member time and pay 1200EUR to get the members trained in advanced level microscopy. In the USA it definitely not going to happen on any significant scale. Maybe a cultural difference :) I recall the comparison provided by US based Zeiss specialist after his training in Germany: "problem= ballpoint pen stopped writing. German style solution = research potential causes, i.e. disassemble the pen, check if any ink is left, if ball rotates freely, finally if the user properly presses knob or rotates the body. This approach may take significant but reasonable time and provide useful skills and experience for the future. American style solution = check the pen price first. As it is much lower than the fee of professional spending hours on this problem then just trash the pen and get a new one" 3. Of course in the USA there are labs that need advanced level microscopy. However, typically they have their own microscopes and microscopy experts and very rarely use Core services. Among my 400 trainees over 15 years only about 10 expressed willingness to proceed to the advanced level, and among them only 1 grad student. 4. Finally, time and cost considerations. At 1 month and 1200EUR spending level how many techniques a beginner researcher may learn in 1 year ? 3years? BTW I think training in optical/confocal microscopy is relatively inexpensive, preparing your own samples typically is more time consuming and expensive, and learning other tecniques may be more expensive as well. My 2 cents, Arvydas +++++++++++++++++++++++++++++ Arvydas Matiukas, Ph.D. Manager of NRB Shared Equipment Director of Confocal&Two-Photon Core SUNY Upstate Medical University Neuroscience & Physiology Dept >>> Steffen Dietzel <[hidden email]<mailto:[hidden email]>> 10/17/2019 6:55 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Flists.umn.edu-5Fcgi-2D2Dbin-5Fwa-2D3FA0-2D3Dconfocalmicroscopy-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253Dk0lBHjfBdP6gkIAbpZx0fMuOqb1oDSvuwF7Hk7nelgY-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3D33-252B4-252FtGWwiqvsmKiNzGAW0aukHIA2-252FpAmrsZ9VBSjMM-253D-26amp-3Breserved-3D0-3D&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=gYoyLgrURo1RALI4SegLkCiZnA47MYISRyzpGspxA2Y&e= Post images on https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Fwww.imgur.com-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253DyAGFeShn7KSa-2DeW2mPu6XbhS9SeX7BD0-2DL0UF5cyjgE-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3DZZTTcrCt02Ajc2FRmgIqS-252B02bjk1fr-252FsdS2sD7XeCJk-253D-26amp-3Breserved-3D0-3D&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=r4ZtumrZCH_QPcENYs9Rjej_QEdlGul0I7tbNlSAWWM&e= and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttps-2D3A-5F-5Fwww.bioimaging.bmc.med.uni-2D2Dmuenchen.de-5Flearn-5F-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253DZ2jynrNg35the3m1H5X3AsUA52lnDqOb4qZUaoY05dY-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3DwTlu4BQcx4umweBHRlkLTKN0GgUDShIwPDTI6-252F91GmU-253D-26amp-3Breserved-3D0-3D&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=ao9M8qvYszkRSrM9hlBecorhSDu4rZXrwLI95tWLmP8&e= Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Flists.umn.edu-5Fcgi-2D2Dbin-5Fwa-2D3FA0-2D3Dconfocalmicroscopy-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253Dk0lBHjfBdP6gkIAbpZx0fMuOqb1oDSvuwF7Hk7nelgY-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3D33-252B4-252FtGWwiqvsmKiNzGAW0aukHIA2-252FpAmrsZ9VBSjMM-253D-26amp-3Breserved-3D0-3D&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=gYoyLgrURo1RALI4SegLkCiZnA47MYISRyzpGspxA2Y&e= > Post images on https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Fwww.imgur.com-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253DyAGFeShn7KSa-2DeW2mPu6XbhS9SeX7BD0-2DL0UF5cyjgE-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657735871-26amp-3Bsdata-3DJGUsYIzKk6PJ2T5jsCarT8P8Xn-252B4Dd7MqUtQMJqA-252B28-253D-26amp-3Breserved-3D0-3D&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=e-M4FS8r15B42EbzdZ3bIcMr2JD6fJfVTJEi_WN9S7k&e= and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität Mnchen Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Fwww.bioimaging.bmc.med.uni-2D2Dmuenchen.de-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253DGadulq06lK7kYY94plB3DsipjiGMo01xTiPc-5FpoN3-5FY-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657735871-26amp-3Bsdata-3DAmakLWZCfe8xWPdce1PWi-252FuB53USTGlG3c612J1-252FNsM-253D-26amp-3Breserved-3D0-3D&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=m0tTsUQhJ8ADQ-i1GcUKVY7XXqVixk1QW6IwpObYRd0&e= När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=I_ZEPCfxNjTx3FqHHR_fr3SyZFn3FdjKs589aCzGAlU&e= >. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI&s=K9vglLP5oMXYKSUvST7m7FHgGSj1XdI2DIbDZ7SeRM4&e= >. |
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Sylvie Le Guyader |
Re: Confocal and Widefield microscopy training material
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In reply to this post by Roberts,Owen S
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** And I would say that the users of the fv1000 probably fair better after your training than their colleagues who travel far to the other microsope! I love your approach! :) Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Neo, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! On 18 Oct 2019 18:33, "Roberts,Owen S" <[hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002490947&sdata=weqPwtRiJ5SyBUz9XT67Qi3ktVxmuds79skK9UGpN1c%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002490947&sdata=rDsoD%2Ff2RdqWl9cIFh54uDETE%2BH09Zg4Xd%2BiP9%2BDSYc%3D&reserved=0 and include the link in your posting. ***** First a bit of an introduction as I am new to the list, but have consulted it for years. I am caretaker of a Olympus FV1000 with a mix of DPSS, Ion, and Diode lasers, with two AOTF filters on a BX61 Frame. I'm an electronics specialist who maintains the microscope, deals with the vendors, and trains the students. Our Confocal was purchased for corrosion research, but that took another direction. Having a background in Ion lasers, spectroscopy, high speed, and infrared imaging, my manager decided I should pick up the confocal when I joined the department. So while we consider the confocal a department instrument, most of our users are graduate biology and biomedical engineering students. Our policy is to graciously share, as the microscope is depreciated, having sat with very little use for a long time. This is an auxiliary duty for me, and runs "Time Available", so training occurs at a mutually agreeable time and day, so I can take care of my primary duties. I ask PIs to donate the main consumable, the arc lamps. The argon laser operates at a very low current, so the tube life will be very long, as I take care to enforce warm-up and shut down schedules to prolong tube life. Having looked at the costs and time involved to bill internally, its actually less cost for us to just leave it open, as it is not in use on a daily basis. We have several institutions near by that have instruments and charge a low rate for their use, so PIs have a choice of a modern, more sophisticated instrument at 30$ or so an hour, or the FV1000. When they have a good grant they use an instrument about 20 miles away, otherwise they use ours. So my trainees usually graduate to a more modern instrument at some time. Training consists of about six "one on one" or "small group" hours with me, with a break for lunch. It consists of some basic optics, hardware, and fluorescence theory, rigorously enforced procedures to protect the instrument, and imaging of some carefully constructed test and calibration slides using a variety of phosphors and polymers to simulate dyes. I keep a PMT tube around as a training aide as well. If a student has a sample prepared, they are welcome to bring it. One of my goals is to break the theory that some PIs have that a set of notebook based settings, handed down, will work with every sample. So we work on finding an image and optimizing it. I'll then schedule a session with each student and their own samples. If I'm convinced they are progressing, they get a "License to Drive", and an introduction to our scheduling calendar. I'll then gradually wean them off of having me present. They have my cell number and I'll check in at random to see how they are doing. I have great freedom over what I do, and I generally can get back to the microscopy lab in less then ten minutes if they are having an issue. If a student is using the wet objectives, or tiling, I'll do a another full day with them. Only two students in five years have needed me full time to do their imaging for them. My degree is in secondary education, so I use a scaffold learning process to teach students self confidence in using the instrument. As we are a College of Engineering, next to a College of Polymer Science, often I get requests to help with non-standard or new methodologies, and we push the machine to its limits some times. Quantum Dots, Corrosion Samples, Hydrogels, things that are out of the biological realm, get my full time attention. The AOTFs enable these areas of research. No tests, no quizzes, some optional pre-reading on their own time, and personalized help. I'm not a biologist, so if I get stuck, I reach out to those who might know. Steve Steve Roberts Senior Technician, Instrumentation The Department of Chemical and Bio-Molecular Engineering The University of Akron Cell: 330-245-7571 ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Straatman, Kees (Dr.) <[hidden email]> Sent: Friday, October 18, 2019 3:59 AM To: [hidden email] <[hidden email]> Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002490947&sdata=weqPwtRiJ5SyBUz9XT67Qi3ktVxmuds79skK9UGpN1c%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002500944&sdata=tpk67Q%2FOrqfVcwxoLJO1Btx%2B%2FJWB6XZ7zQOa5RMJGTc%3D&reserved=0 and include the link in your posting. ***** Very interesting discussion. Here in Leicester I train users in 3.5 hour 1 to 1 sessions on the microscope they want to use and with their sample, so I can show them how to setup the microscope and optimize it for their use. If I am happy with their progress they get an account and the next time they want to use the microscope they have to make an appointment with me or bring an experienced user from their group, so we are sure they are able to start the system and get an image on the screen. After that it is up to the user. I also would not have the time/resources to spend more time on training each individual user. My facility is spread over 4 buildings so I am not always around to guide and solve problems, but I walk in and try to keep an eye on their progress. Occasionally I have a user that fails the 'progress test' and they will get a second induction session. If I am still not happy with their ability to use the microscope they have to make appointments with me to do their imaging. With some users I do the imaging or I keep a close eye on their imaging because their subject is difficult and there are some user who want me to do their imaging and they always can make an appointment with me to sit-in on one of their imaging sessions. If I do the imaging it is a collaboration and I would be co-author on any paper resulting from this work. This is charged on the standard rate of using a microscope for internal users, external users have to pay for my hours as well. Qua teaching the facility is involved in several courses. I think it is always good if the students know we are around as they are potential future users. None of the lectures bring in money, nor undergrad use of the microscopes during courses but postgraduate courses on the microscopes get charged for hourly use of the system. Best wishes Kees Dr Ir K.R. Straatman Senior Experimental Officer Advanced Imaging Facility Centre for Core Biotechnology Services University of Leicester https://eur01.safelinks.protection.outlook.com/?url=www.le.ac.uk%2Fadvanced-imaging-facility&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002500944&sdata=pVbLa4BjKpul6X1K9ZtYN9O0jWLT0P%2BuxhJhkiNkd3Q%3D&reserved=0 ImageJ workshops 16 and 17 December https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww2.le.ac.uk%2Fcolleges%2Fmedbiopsych%2Ffacilities-and-services%2Fcbs%2FAIF%2Fworkshops%2Fimagej-workshops-December-2019&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002500944&sdata=dh%2BfWzExGkp4QHXGzYsy0o6dGCTWR7cP6S9xnsVXH40%3D&reserved=0 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Cammer, Michael Sent: 17 October 2019 21:38 To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002500944&sdata=vde1h2QvmV7FqPncw2UUjocZqQFvNi%2FesiN4Rl%2F99jM%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002500944&sdata=tpk67Q%2FOrqfVcwxoLJO1Btx%2B%2FJWB6XZ7zQOa5RMJGTc%3D&reserved=0 and include the link in your posting. ***** I have pinch hit lectured and ran labs in many courses. Sometimes the instrument time was paid for out of an education budget, but mostly not. The core (or me personally since most lectures were prepared at home) absorbed the costs of my time. I also taught two years of a week-long course for new grad students. I sent bills, but don’t know if they were actually paid. Teaching does bring in business. Students who find our teaching useful or interesting spread the word. Cheers- Michael Cammer -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Oshel, Philip Eugene Sent: Thursday, October 17, 2019 3:47 PM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002500944&sdata=ghSNgw3b%2Fjs6WDUBDdwRgiwY6REZtXw3JoDghMIlf9o%3D&reserved=0= Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002500944&sdata=AvKse9cLTfxwQPA2m0zdWsEaW7YCVW%2Ft%2FPiKwzDfQf0%3D&reserved=0= and include the link in your posting. ***** We teach a grad-level microscopy course in confocal (also SEM & TEM courses). Tuition dollars don't come to the facility, but the instrument use (student use hours) is billed to the course, and that money does come to the facility. The instruments are billed at the standard "in-house" rate. Note: This are the same courses the undergrads in our "microscopy major" take. Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office -----Original Message----- From: Confocal Microscopy List <[hidden email]> on behalf of "Phillips, Thomas E." <[hidden email]> Reply-To: Confocal Microscopy List <[hidden email]> Date: Thursday, 17October, 2019 at 15:34 To: "[hidden email]" <[hidden email]> Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002510937&sdata=79BAYE5HZ%2BJSb%2BzoSfO3SuHHS5fF2OYiH9mBylN2Ufo%3D&reserved=0= Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002510937&sdata=sGslEFxSCB4zuSrl1%2BBNjEdKDcF%2F%2BjWO2ydhX3JRaDc%3D&reserved=0= and include the link in your posting. ***** Does anybody offer their confocal training as an official grad-level class? In the past, offering a hands-on class was problematic for us since (i) no tuition dollars flow back to the core and (ii) PI's wanted their students using their own samples so "free class time" meant no income for us when those projects would normally have been done outside of class. Our campus is moving to a new budget model and tuition dollars may flow more directly back to us. We are considering combining training and supervised independent use as part of the class design and allow student efforts to be credentialed. I would be interested to know if anyone else is taking this approach and how many credit hours you offer and what you cover as part of the course. I have never met a PI who would have paid for Sylvie's design and impressed her clients do so. Thomas E. Phillips, Ph.D Professor of Biological Sciences Curators’ Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email] https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.biology.missouri.edu_faculty_phillips.html%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3D2X2JzDknmfdEUySiYs80Xwo3E8_H7XOtsRvsqjyk-as%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002510937&sdata=HFSLhFFz6oMrtTopi1VYWmsIEtW5YGvTT2w6srZH8Z8%3D&reserved=0= https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__microscopy.missouri.edu%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DnH6sgQk_G8l8jC3aTU7fVEdy8GAniz_FC3AxsckNPMs%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002510937&sdata=rCkLRAje52yxsS36PvJFxdxlFFLBQe6rY3WtrvYsUyA%3D&reserved=0= -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Kumar, Niyanta Sent: Thursday, October 17, 2019 2:12 PM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002510937&sdata=79BAYE5HZ%2BJSb%2BzoSfO3SuHHS5fF2OYiH9mBylN2Ufo%3D&reserved=0= Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002510937&sdata=sGslEFxSCB4zuSrl1%2BBNjEdKDcF%2F%2BjWO2ydhX3JRaDc%3D&reserved=0= and include the link in your posting. ***** Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel Sent: Thursday, October 17, 2019 6:55 AM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material EXTERNAL EMAIL – Use caution with any links or file attachments. ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002520933&sdata=TE9YLs1UQjIBA4iDkbc67ya0RdZVIjvLv2Vmxvz2JN8%3D&reserved=0= Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002520933&sdata=42jospPS3VAjhDg6cqJv0lR8uHw5hLqKsg%2FxdzEU8v4%3D&reserved=0= and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_learn_%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3D8ICIFmvnIJoBPS2mAi7Ci-kXZCRIY5Bv8knrcEHDEtQ%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002520933&sdata=kdHgybVJMvBh4ngtTReTANAVnkiXs50aM%2BVVo3BTA4o%3D&reserved=0= Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DAQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002520933&sdata=TE9YLs1UQjIBA4iDkbc67ya0RdZVIjvLv2Vmxvz2JN8%3D&reserved=0= > Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DMal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002520933&sdata=42jospPS3VAjhDg6cqJv0lR8uHw5hLqKsg%2FxdzEU8v4%3D&reserved=0= and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de%26d%3DDwIGaQ%26c%3Dj5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs%26r%3DhUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM%26m%3DzfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY%26s%3DXjRa4ovswLBxD4kAWsQfoP7jxzMCqpm5soZBwmqyrBQ%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C32e7218be5364cb91a0208d753e8e17d%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070132002520933&sdata=MayKeF6R84sY944EKc43nP9vw3h4OskhcNUHm30cuxY%3D&reserved=0= Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. 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Sylvie Le Guyader |
Re: Confocal and Widefield microscopy training material
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In reply to this post by Arvydas Matiukas
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** ' Nikon software (that Sylvie mentioned) is very flexible but not very user friendly while Zeiss is much more intuitive and 3 hrs may be enough (no commercial interest, just my personal opinion)' I agree. We get around this by having a common layout in the Nikon software with all the tabs that are needed. Our videos are made with that default layout. It ends up being very similar to the Zeiss software + the added flexibility. We also have a Zeiss confocal and indeed we often take less time to train users. However we actually book the same amount of time (5x3h in 1 week). This means that we have room for customization of the training in case the sample is very demanding. If not, the user simply uses the time to acquire their data since they have lots of samples. Regardless of which system we train the users on, we always make sure that they have ample time during and the week following the training to work on their own. This helps a lot in anchoring the newly acquired knowledge so that it sticks. It also helps in creating a relationship with the user. Since they get used to coming to our office to tell us when they have finished a task during the training, it feels very natural for them to come and grab us later if they have a question or have forgotten something. I think that this is a big part of setting up a culture where users do not hesitate to come and ask which avoids lots of trouble. Maybe you could say that we have 12h of training + 3h of the user practicing different tasks on their own? But they have to book 15h in one week and make lots of sample anyway. 😃 Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Arvydas Matiukas Sent: 18 October 2019 19:38 To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315436396&sdata=SvKfxkaoOxjSwVM10YeGcWOAbGHZf6WZGtLgMm0GcDA%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315436396&sdata=DceUhZV6RwY9KizdVg4XwGl63R2pLAiUnaB9xUJVCGs%3D&reserved=0 and include the link in your posting. ***** The further the more deep and interesting the discussion gets! With the details provided I see that Silvie's business plan and microscopy education approach matches the university environment and policy. And I wish her the best luck spreading good quality microscopy education. However, not every Core is immersed in environment of that large scale and wisely organized . Remove undergrads, Excellence Center status, scale instrument # at 20%, student / lab # and finances/resources at 10% - you will get our environment in which we still need to survive adapting our policies . Regarding min training hrs required for the trainee to be able to run confocal without supervision. It also depends on the "user friendliness" of the software. Nikon software (that Silvie mentioned) is very flexible but not very user friendly while Zeiss is much more intuitive and 3 hrs may be enough (no commercial interest, just my personal opinion) . Best regards, Arvydas Click to add a signature ( gwsig:addsig) >>> Sylvie Le Guyader <[hidden email]> 10/18/2019 4:42 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI%26s%3DSGQ43Lb3oE49o-xeu2RvxrdKC8nNB2LdqthBVQYxcSc%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315436396&sdata=en9Adm17lb1KV%2BfbFMqUE7qmJaJ9ezAtOHYVSmVyLCE%3D&reserved=0= Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.imgur.com%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI%26s%3Da4uHDqqZ1djwdyonkSuqCBiEBwBX6vXQoi6fFvwKnaE%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315436396&sdata=oV2Y1o5Xf2EQnACMfhpt2FgjjMCGlPDWghV5A8WVkvI%3D&reserved=0= and include the link in your posting. ***** Dear listers A long answer but, sorry, I got a bit agitated. How did such an enormous distortion of what I wrote happen? I never said that we train people for one month! 1h discussion + 5x3h training is what we do. How did this one-month thing come up? And during the 5x3h, the user has ample time to practice what we just taught her/him before we add one more layer. A lot of facility staff agree that 3h is totally not enough, many do 2-3 sessions of 3h, we add 2 more sessions and tons of value. And then I do not suppose that you start with sitting at the microscope and showing how to set the gain without having any idea what the sample/experiment will be, or do you? Well that part comes first and last 1h. Nothing outrageous! And this is no advance training. This is our basic. I do believe that at the end of a confocal training the user should know how each setting on the confocal can influence their results and they should have enough knowledge to make decisions on their own. If you buy a confocal, you get trained by the company and they explain things like pixel size, resolution, saturation, averaging, z stack, multidimension... That lasts longer than 3h. At least all the trainings I got from vendors did and I would not accept to get trained in 3h for a complex machine that costs several hundreds of thousands of dollars, would you? Why would a facility business model aim at delivering a worse training than companies who have no knowledge about/daily contact with samples and research as we facility people do? This totally doesn’t make sense to me. Our business model is that we educate our users so that they can redesign their experiments when their research requires without asking us which new settings they should use. But they anyway often ask for us to confirm their settings when they do that, because they know that, even if they have been using the facility for years and are very experienced, we can come up with great ideas and suggestions that will help them a lot. And we do this for free. They can get help for free from us any time after their training. If they go on parental leave for 1 year and come back, they get a free refresher (there go another 3h!). I believe that we facility people must present ourselves to researchers and group leaders as experts in microscopy experiment design. Because this is what we facility people are. We pile up the knowledge from tons of experiments and samples. We are a gold mine for researchers! During the first hour of discussion (for free) with our future users (and often their PIs), we do several things: * troubleshoot their sample prep and experimental design * open their eyes to the limits of what our equipment can deliver but also explain how much more they can do with our equipment compared to what they had initially planned * very importantly: we really brainstorm their experiment and team up with them during that time. When we are done, they leave with lots of free tailor-made information. They could then decide to go to another facility to work if they wanted. They have not paid anything yet. Instead, when they go, they always thank us greatly for our time, look at each other talking about all the details they need to change and realize that if they collaborate with us (this is the way I see our role as a facility, even if we are not on papers), their project will take a huge leap forward. They will gain tons of time and get meaningful data. Which group leader would NOT want to pay 1200 euros to save months of salaries and gain the assurance that their data is trustworthy? Why design a training to leave the trainee knowing how to push a few buttons but having to reinvent the wheel (and likely forgetting the spokes) about everything else that microscopy is? What I shared with you in my previous post (workflow of how to set a confocal) is not the workflow of our training, it is only the conclusion of the button-pushing part. This is a summary that is freely available on our website (under teaching material) and that we go through with them at the end to make sure they are very confident with answering all these questions for their sample/experiment. To me these points represent a minimum requirement, not an advanced training. These questions are not even half of the content of our basic training. Most of what we talk about is sample prep, experimental design and image analysis strategies. Not surprisingly to me, no one ever complains about the price and time. Btw, we also have a yearly microscopy course for PhD students and postdocs. The full schedule for 2019 is on our website and the 2020 will be up soon. All lectures are broadcasted live for free without registration. Varsågod as one says in Swedish. We also welcome people from other facilities who want to shadow us (for free) or be trained by us (for the same fee as everyone). This year we trained people from Poland and Turkey that way. We are a university. Our role is to deliver the best possible education and research to society. To help everyone compare to their own facility, here are a few numbers about us: - we have 9 advanced microscopes with all sorts of functions (11 year-old facility, Nikon Center of Excellence since 2014) - about 90 users from 50 different groups (and shades of samples) - we are 2.8 persons (director 10%, manager 100%, research engineer 100%, research engineer 50%, computer scientist 20%) - our training (which we call Entry) and Usage fees cover 37% of our total budget including all salaries, maintenance and upgrading machines, only excluding purchasing new machines for which we need to get dedicated grants. - We also have an unusual fee model: our users pay one Usage fee per year (or 6, 3 or 1 months) which currently costs 18000 euros per year and there is no additional hourly fee. All booking rules and such are found on our website/using the facility/guidelines for those who want to know more. Less boring admin, more time for trainings and fun collaborations! And unlike Kees, we never acquire images for anyone. If people do not have time to get trained, they collaborate with one of our users. And we have the advantage that our systems are all in the same room so we are probably less trained that he is! All comments about us are very welcome. I especially welcome comments about any mistake you find on our website/training material/during our course. I strongly believe in lifetime learning. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Arvydas Matiukas Sent: 17 October 2019 20:37 To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Flists.umn.edu-252Fcgi-2Dbin-252Fwa-253FA0-253Dconfocalmicroscopy-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3DUw495XDm93PClu4a0Ge61sjaV9DIw3Bqw5wqdxZcyJ0-253D-26amp-3Breserved-3D0%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI%26s%3DqjVl4iRRCGRDTthYRfxqZeAAoo93FWJhGf9jz50jlt0%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315436396&sdata=gxSzgwAnFn%2BixVaW9vdhsgYf7j%2FcpIuSAXTKr9Fuono%3D&reserved=0= Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Fwww.imgur.com-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3DdpL2GOaVTKVxvFO7y-252F93U-252FGDxNcZyIQT-252BUPlMMR3V9Y-253D-26amp-3Breserved-3D0%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI%26s%3DXMwNbMdDPanoMyqohSt6QY7IAzBiIhAon5c5Fn6K5lI%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315436396&sdata=ZrNiqLDin2hFsAgQkfGSb45iUfRbzz7%2FSYrn%2BfTbVUE%3D&reserved=0= and include the link in your posting. ***** Hi Steffen, Silvie Thanks for very interesting and useful discussion. Few summary thoughts: 1. I second Steffens' reasoning for starting training with Core provided=known=calibrated samples. Sylvie's approach of starting with users'=non calibrated sample introduces to many unknowns at a time and to my opinion reduces efficiency of the training. I personally prefer not to be trained in such stressful way. 2. I wonder if many labs in EU can afford to spend 1 month of their member time and pay 1200EUR to get the members trained in advanced level microscopy. In the USA it definitely not going to happen on any significant scale. Maybe a cultural difference :) I recall the comparison provided by US based Zeiss specialist after his training in Germany: "problem= ballpoint pen stopped writing. German style solution = research potential causes, i.e. disassemble the pen, check if any ink is left, if ball rotates freely, finally if the user properly presses knob or rotates the body. This approach may take significant but reasonable time and provide useful skills and experience for the future. American style solution = check the pen price first. As it is much lower than the fee of professional spending hours on this problem then just trash the pen and get a new one" 3. Of course in the USA there are labs that need advanced level microscopy. However, typically they have their own microscopes and microscopy experts and very rarely use Core services. Among my 400 trainees over 15 years only about 10 expressed willingness to proceed to the advanced level, and among them only 1 grad student. 4. Finally, time and cost considerations. At 1 month and 1200EUR spending level how many techniques a beginner researcher may learn in 1 year ? 3years? BTW I think training in optical/confocal microscopy is relatively inexpensive, preparing your own samples typically is more time consuming and expensive, and learning other tecniques may be more expensive as well. My 2 cents, Arvydas +++++++++++++++++++++++++++++ Arvydas Matiukas, Ph.D. Manager of NRB Shared Equipment Director of Confocal&Two-Photon Core SUNY Upstate Medical University Neuroscience & Physiology Dept >>> Steffen Dietzel <[hidden email]<mailto:[hidden email]>> 10/17/2019 6:55 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Flists.umn.edu-5Fcgi-2D2Dbin-5Fwa-2D3FA0-2D3Dconfocalmicroscopy-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253Dk0lBHjfBdP6gkIAbpZx0fMuOqb1oDSvuwF7Hk7nelgY-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3D33-252B4-252FtGWwiqvsmKiNzGAW0aukHIA2-252FpAmrsZ9VBSjMM-253D-26amp-3Breserved-3D0-3D%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI%26s%3DgYoyLgrURo1RALI4SegLkCiZnA47MYISRyzpGspxA2Y%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315446391&sdata=%2FqsySNY3e4uSbQWdxd9qNrkn3wKb9G1SntiqY0itpx4%3D&reserved=0= Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Fwww.imgur.com-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253DyAGFeShn7KSa-2DeW2mPu6XbhS9SeX7BD0-2DL0UF5cyjgE-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3DZZTTcrCt02Ajc2FRmgIqS-252B02bjk1fr-252FsdS2sD7XeCJk-253D-26amp-3Breserved-3D0-3D%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI%26s%3Dr4ZtumrZCH_QPcENYs9Rjej_QEdlGul0I7tbNlSAWWM%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315446391&sdata=m8yZlhKv6rSXTlAq0SMWwj1h8gKUghaEXuwxtf1WY5c%3D&reserved=0= and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttps-2D3A-5F-5Fwww.bioimaging.bmc.med.uni-2D2Dmuenchen.de-5Flearn-5F-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253DZ2jynrNg35the3m1H5X3AsUA52lnDqOb4qZUaoY05dY-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3DwTlu4BQcx4umweBHRlkLTKN0GgUDShIwPDTI6-252F91GmU-253D-26amp-3Breserved-3D0-3D%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI%26s%3Dao9M8qvYszkRSrM9hlBecorhSDu4rZXrwLI95tWLmP8%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315446391&sdata=StTqftXEFe%2B0vL3UzFj2CZwFlGT2AHx%2BzplNbNQ6gLA%3D&reserved=0= Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Flists.umn.edu-5Fcgi-2D2Dbin-5Fwa-2D3FA0-2D3Dconfocalmicroscopy-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253Dk0lBHjfBdP6gkIAbpZx0fMuOqb1oDSvuwF7Hk7nelgY-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657725877-26amp-3Bsdata-3D33-252B4-252FtGWwiqvsmKiNzGAW0aukHIA2-252FpAmrsZ9VBSjMM-253D-26amp-3Breserved-3D0-3D%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI%26s%3DgYoyLgrURo1RALI4SegLkCiZnA47MYISRyzpGspxA2Y%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315446391&sdata=%2FqsySNY3e4uSbQWdxd9qNrkn3wKb9G1SntiqY0itpx4%3D&reserved=0= > Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Fwww.imgur.com-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253DyAGFeShn7KSa-2DeW2mPu6XbhS9SeX7BD0-2DL0UF5cyjgE-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657735871-26amp-3Bsdata-3DJGUsYIzKk6PJ2T5jsCarT8P8Xn-252B4Dd7MqUtQMJqA-252B28-253D-26amp-3Breserved-3D0-3D%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI%26s%3De-M4FS8r15B42EbzdZ3bIcMr2JD6fJfVTJEi_WN9S7k%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315446391&sdata=A5quxz06GcZFUnPrn1c%2Fx%2BUP1uRcpiqPTk1Kf9VRxrA%3D&reserved=0= and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität Mnchen Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Fwww.bioimaging.bmc.med.uni-2D2Dmuenchen.de-2526d-253DDwIDaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253D99s-5FHGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0-2526s-253DGadulq06lK7kYY94plB3DsipjiGMo01xTiPc-5FpoN3-5FY-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C189366ab217145c341dc08d75332bac7-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637069349657735871-26amp-3Bsdata-3DAmakLWZCfe8xWPdce1PWi-252FuB53USTGlG3c612J1-252FNsM-253D-26amp-3Breserved-3D0-3D%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DywZakhRrmOA-JFHPuuciFeExQbJ2LQ4SBIVOyGVquRI%26s%3Dm0tTsUQhJ8ADQ-i1GcUKVY7XXqVixk1QW6IwpObYRd0%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7Cd253387701ce4042cc2508d753f20915%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637070171315446391&sdata=CRBCu7t3hPkQdyjlD5RtwAd3XuoMFRusGaLppSizGjM%3D&reserved=0= När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. 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Steffen Dietzel |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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In reply to this post by Sylvie Le Guyader
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Sylvie, Am 18.10.2019 um 10:42 schrieb Sylvie Le Guyader: > How did such an enormous distortion of what I wrote happen? I never said that we train people for one month! 1h discussion + 5x3h training is what we do. How did this one-month thing come up? And during the 5x3h, the user has ample time to practice what we just taught her/him before we add one more layer. I think somebody mixed up your training length (which you did not specify in your 16.10.2019, 18:34 e-mail) with the length of your microscope course of 1 month that you mentioned at the end of that e-mail. You did explain the training length in the parallel thread (training and bets practices for confocal, 11.10.) but not everybody reads every thread, so that was probably not in everybody's mind. I think that what you do is pretty close to how it ideally should be. But I don't see how we could do it that way with the resources we have. > Which group leader would NOT want to pay 1200 euros to save months of salaries and gain the assurance that their data is trustworthy? Why design a training to leave the trainee knowing how to push a few buttons but having to reinvent the wheel (and likely forgetting the spokes) about everything else that microscopy is? Well, I guess some group leaders would not think that it wouldn't save them months, because it also worked in the past without it. Plus, there is a tendency sometimes (also from the users themselves) that they just want to get their data as quickly as possible without looking left or right, when microscopy is just one of many methods to be used. I can't even blame them, when, say, a biochemist is forced by reviewers to show that his protein has a certain location in the cell, that s/he wants to get done as quickly as possible. Of course this is far from the ideal situation, from a microscopist point of view. One of the issues we are having here is that traditionally, using microscopes was "free", meaning somebody got it through a grant and you were allowed to use it without usage fee. Until repairs were due, when it was suddenly not so free anymore. And training was done by somebody who happened to know how to work with the instrument. Sometimes on a very high level. And sometimes not. From this culture of free microscopy we now have to move to a pay-by-the-hour routine. And I believe we need to do this in several steps, not one big one. If we were to charge a user 18 k€ per year, we would loose most of our users, they couldn't afford it any more. One the other hand, we have one advantage, we don't have to refinance the salaries from our fees. So for a training, the issue is not really how much we charge, but how much time we can make available for it. Thank you, Sylvie, for the detailed numbers for comparison. Best Steffen -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany http://www.bioimaging.bmc.med.uni-muenchen.de |
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Sylvie Le Guyader |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I would recommend applying fees straight away. In 2014, we did actually jump from no fee for nothing (training and usage) to training fee 1200€ (has not changed) and usage fee 1200€/year (which has progressively increased to 1800€, not 18 k€ though). I was working alone then. In 2013, I trained 42 people with a rather extensive training. I was exhausted! In 2014, the numbers of users and of trainings were divided by 2 but we got an income from it for the first time. I got lucky to have a 50% person to help me with troubleshooting. Every year after the fees were introduced the number of training increased sharply until in 2017 we were higher than 2013. Luckily by that time, I got one more person to work 100%. I would say that at least 2/3 of the trainings that we 'lost' in 2014 were people who were either tourists (simply curious or interested in learning but no application or no sample) or people who had a very short application (a few images to re-acquire to resubmit a paper). Tourists are only a waste of everyone's time, including their own. I am happy we got rid of them. It is very rare now that we train someone who doesn’t immediately start using our system intensively. People who need to grab a few images quickly and that's it are told to collaborate with one of our trained users, for everyone's benefits. It might be different in different countries but in Sweden, it is practically impossible these days for 1 lab to get a grant to buy a confocal. The focus is on facilities. That helps us for sure. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel Sent: 21 October 2019 10:17 To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C7b86a6d5cc6446af7a3008d756024138%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637072439995747207&sdata=4SF1QOuYSi%2Fz%2BwygEmNnhWL7aaf7adZSHTGMTKdjkCU%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C7b86a6d5cc6446af7a3008d756024138%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637072439995757202&sdata=CPy%2B3KKXl01I2Uowq3SYxqOcQK%2B11KcmAy%2BENC4Jekg%3D&reserved=0 and include the link in your posting. ***** Hi Sylvie, Am 18.10.2019 um 10:42 schrieb Sylvie Le Guyader: > How did such an enormous distortion of what I wrote happen? I never said that we train people for one month! 1h discussion + 5x3h training is what we do. How did this one-month thing come up? And during the 5x3h, the user has ample time to practice what we just taught her/him before we add one more layer. I think somebody mixed up your training length (which you did not specify in your 16.10.2019, 18:34 e-mail) with the length of your microscope course of 1 month that you mentioned at the end of that e-mail. You did explain the training length in the parallel thread (training and bets practices for confocal, 11.10.) but not everybody reads every thread, so that was probably not in everybody's mind. I think that what you do is pretty close to how it ideally should be. But I don't see how we could do it that way with the resources we have. > Which group leader would NOT want to pay 1200 euros to save months of salaries and gain the assurance that their data is trustworthy? Why design a training to leave the trainee knowing how to push a few buttons but having to reinvent the wheel (and likely forgetting the spokes) about everything else that microscopy is? Well, I guess some group leaders would not think that it wouldn't save them months, because it also worked in the past without it. Plus, there is a tendency sometimes (also from the users themselves) that they just want to get their data as quickly as possible without looking left or right, when microscopy is just one of many methods to be used. I can't even blame them, when, say, a biochemist is forced by reviewers to show that his protein has a certain location in the cell, that s/he wants to get done as quickly as possible. Of course this is far from the ideal situation, from a microscopist point of view. One of the issues we are having here is that traditionally, using microscopes was "free", meaning somebody got it through a grant and you were allowed to use it without usage fee. Until repairs were due, when it was suddenly not so free anymore. And training was done by somebody who happened to know how to work with the instrument. Sometimes on a very high level. And sometimes not. From this culture of free microscopy we now have to move to a pay-by-the-hour routine. And I believe we need to do this in several steps, not one big one. If we were to charge a user 18 k€ per year, we would loose most of our users, they couldn't afford it any more. One the other hand, we have one advantage, we don't have to refinance the salaries from our fees. So for a training, the issue is not really how much we charge, but how much time we can make available for it. Thank you, Sylvie, for the detailed numbers for comparison. Best Steffen -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.bioimaging.bmc.med.uni-muenchen.de&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C7b86a6d5cc6446af7a3008d756024138%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637072439995757202&sdata=pfWbmOjRrVNorAbXzI7e3ZYDmicywVte%2BdoeAQhXUDE%3D&reserved=0 När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>. |
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Michelle Peckham |
FW: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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In reply to this post by Neftali Flores Rodriguez
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** And another possible contact? On 17/10/2019, 05:34, "Confocal Microscopy List on behalf of Neftali Flores Rodriguez" <[hidden email] on behalf of [hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Niyanta, You might find our MyScope useful. https://myscope.training/ Neftali Flores-Rodriguez | Light Microscopist Sydney Microscopy and Microanalysis (SMM) Australian Centre for Microscopy & Microanalysis (ACMM) THE UNIVERSITY OF SYDNEY ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Kumar, Niyanta <[hidden email]> Sent: Wednesday, 16 October 2019, 6:04 pm To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu Post images on https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com and include the link in your posting. ***** Thanks Sylvie, this is helpful! Niyanta Kumar, Ph.D. Senior Scientist - Pharmacokinetics and ADME Merck Research Laboratories -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Sylvie Le Guyader Sent: Wednesday, October 16, 2019 9:55 AM To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material EXTERNAL EMAIL – Use caution with any links or file attachments. ***** To join, leave or search the confocal microscopy listserv, go to: https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu Post images on https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com and include the link in your posting. ***** ’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’ Definitely! That would bore me too and I am not from the millennium generation! 😉 I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. 😊 Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website<https://protect-au.mimecast.com/s/40xyCMwvLQTAQG37hJE2HF?domain=ki.se> Follow our microscopy blog!<https://protect-au.mimecast.com/s/vt_4CNLwM9iG213zIRmKQP?domain=microscopykarolinska.se> From: Arvydas Matiukas <[hidden email]> Sent: 16 October 2019 13:53 To: Sylvie Le Guyader <[hidden email]>; [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material Hello Sylvie, Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3. with best wishes Arvydas +++++++++++++++++++++++++++++ Arvydas Matiukas, Ph.D. Manager of NRB Shared Equipment Director of Confocal&Two-Photon Core SUNY Upstate Medical University >>> Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://protect-au.mimecast.com/s/k0SUCOMxNyty6oxMcP_F05?domain=urldefense.proofpoint.com Post images on https://protect-au.mimecast.com/s/F4k8CP7yOZtk7qXPIrKiv7?domain=urldefense.proofpoint.com and include the link in your posting. ***** Hi Niyanta Alyona Minina has tons of videos on you tube. Check her channel<https://protect-au.mimecast.com/s/Dn7vCQnzP0ty5mPYc99xjB?domain=urldefense.proofpoint.com <https://protect-au.mimecast.com/s/dOszCROAQotzA8WxT05sni?domain=urldefense.proofpoint.com> >. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Niyanta Kumar Sent: 11 October 2019 22:00 To: [hidden email]<mailto:[hidden email]> Subject: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://protect-au.mimecast.com/s/UPl4CVAGXPtRD1yYIr34h8?domain=urldefense.proofpoint.com Post images on https://protect-au.mimecast.com/s/gH1FCWLJY7iWAvqVFBZlT4?domain=urldefense.proofpoint.com and include the link in your posting. ***** Hi all, Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful. Thanks, Niyanta När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Hr finns information om hur KI behandlar personuppgifter<https://protect-au.mimecast.com/s/08zqCXLKZoizx3KWTx7TnX?domain=urldefense.proofpoint.com <https://protect-au.mimecast.com/s/XRVlCYWL1vi4zPlwurPdw1?domain=urldefense.proofpoint.com> >. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://protect-au.mimecast.com/s/2FL4CZYM2VFp3yg0HVbRoD?domain=urldefense.proofpoint.com <https://protect-au.mimecast.com/s/WnEEC1WZXri97KGlIKjaFh?domain=urldefense.proofpoint.com> >. När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://protect-au.mimecast.com/s/2ZpGC2xZYvC9NA3gIWN0gM?domain=ki.se>. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://protect-au.mimecast.com/s/FH5gC3Q8Z2FE5VKOsP5zcG?domain=ki.se>. Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at https://protect-au.mimecast.com/s/msH2C4QZ1RFvpG5qsQAwax?domain=merck.com that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. 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