Confocal and Widefield microscopy training material

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Niyanta Kumar Niyanta Kumar
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Confocal and Widefield microscopy training material

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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi all,
Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful.
Thanks,
Niyanta
Sylvie Le Guyader Sylvie Le Guyader
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Re: Confocal and Widefield microscopy training material

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Niyanta



Alyona Minina has tons of videos on you tube. Check her channel<https://www.youtube.com/channel/UCfRx0TPF-R1TKYrPPu7OwWA/videos>.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Niyanta Kumar
Sent: 11 October 2019 22:00
To: [hidden email]
Subject: Confocal and Widefield microscopy training material



*****

To join, leave or search the confocal microscopy listserv, go to:

https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C87a9cd3e2b2d42366e3808d74e8707d5%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637064214752285084&amp;sdata=XtByjWY8X%2BLYVFhPt7T7XqnizH6v9I0RZalnJIYo23s%3D&amp;reserved=0

Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C87a9cd3e2b2d42366e3808d74e8707d5%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637064214752285084&amp;sdata=6m3Wpvhetv%2BypOIa61Jr8jZeKn0olhUe50y8LbuvJho%3D&amp;reserved=0 and include the link in your posting.

*****



Hi all,

Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful.

Thanks,

Niyanta



När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.


Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.
Arvydas Matiukas Arvydas Matiukas
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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hello Sylvie,

Thanks for pointing to these video tutorials by Alyona Minina. They
nicely complement traditional powerpoint material and hopefully could
save some my time. I am curious to check how much longer video material
keeps trainee's attention focussed, especially for millenial youtube
generation. I noticed that traditional verbal and powerpoint
presentation makes significant fraction of the basic confocal trainees
bored after 1-2 hrs of total 3.

with best wishes Arvydas






+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University

>>> Sylvie Le Guyader <[hidden email]> 10/16/19 6:28 AM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=eFtCsX4RwhV6lbXscD96MNTyssit68QdB4fZA95hrQQ&e=

Post images on
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=fAjOkG_slD2KBsdz68xI4iL4ibgq5pezOL5F3lRr2y4&e=
 and include the link in your posting.
*****

Hi Niyanta



Alyona Minina has tons of videos on you tube. Check her
channel<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e=
>.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On
Behalf Of Niyanta Kumar
Sent: 11 October 2019 22:00
To: [hidden email]
Subject: Confocal and Widefield microscopy training material



*****

To join, leave or search the confocal microscopy listserv, go to:

https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Flists.umn.edu-252Fcgi-2Dbin-252Fwa-253FA0-253Dconfocalmicroscopy-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C87a9cd3e2b2d42366e3808d74e8707d5-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637064214752285084-26amp-3Bsdata-3DXtByjWY8X-252BLYVFhPt7T7XqnizH6v9I0RZalnJIYo23s-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=9ndwMylZEtR6J8pLSr5vVXCqk8bBKkmliAmW2jootR0&e=


Post images on
https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Fwww.imgur.com-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C87a9cd3e2b2d42366e3808d74e8707d5-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637064214752285084-26amp-3Bsdata-3D6m3Wpvhetv-252BypOIa61Jr8jZeKn0olhUe50y8LbuvJho-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=pZwg3cOxtPEB45-6ZJuKjpBvaR4hMvAION4xiM1-TXU&e=
 and include the link in your posting.

*****



Hi all,

Can someone suggest any existing resources and formats to pull from for
designing new and advanced user trainings for confocal and widefield
microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2
confocal and an Axio Obsever widefield scope. Interactive resources or
existing quizzes that can ensure users know what they need to would alsoNär du skickar e-post till Karolinska Institutet (KI) innebär detta att
KI kommer att behandla dina personuppgifter. Hr finns information om hur
KI behandlar
personuppgifter<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=EQs1kTVGSWHn1Bbnwf8tsm9ZmeiX9rnpVF3Gx8njx2g&e=
>.


Sending email to Karolinska Institutet (KI) will result in KI processing
your personal data. You can read more about KI’s processing of personal
data
here<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=3Bvbvriv0Qsm1rvTGdCTn69BFi2whwW0uFp_0iJEm9g&e=
>.
Sylvie Le Guyader Sylvie Le Guyader
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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’
Definitely! That would bore me too and I am not from the millennium generation! 😉
I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. 😊

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: +46 (0) 73 733 5008
LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility>
Follow our microscopy blog!<http://microscopykarolinska.se/>

From: Arvydas Matiukas <[hidden email]>
Sent: 16 October 2019 13:53
To: Sylvie Le Guyader <[hidden email]>; [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

Hello Sylvie,

Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.

with best wishes Arvydas




+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University

>>> Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=eFtCsX4RwhV6lbXscD96MNTyssit68QdB4fZA95hrQQ&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=fAjOkG_slD2KBsdz68xI4iL4ibgq5pezOL5F3lRr2y4&e= and include the link in your posting.
*****

Hi Niyanta



Alyona Minina has tons of videos on you tube. Check her channel<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e=%20> >.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Niyanta Kumar
Sent: 11 October 2019 22:00
To: [hidden email]<mailto:[hidden email]>
Subject: Confocal and Widefield microscopy training material



*****

To join, leave or search the confocal microscopy listserv, go to:

https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Flists.umn.edu-252Fcgi-2Dbin-252Fwa-253FA0-253Dconfocalmicroscopy-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C87a9cd3e2b2d42366e3808d74e8707d5-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637064214752285084-26amp-3Bsdata-3DXtByjWY8X-252BLYVFhPt7T7XqnizH6v9I0RZalnJIYo23s-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=9ndwMylZEtR6J8pLSr5vVXCqk8bBKkmliAmW2jootR0&e=

Post images on https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Fwww.imgur.com-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C87a9cd3e2b2d42366e3808d74e8707d5-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637064214752285084-26amp-3Bsdata-3D6m3Wpvhetv-252BypOIa61Jr8jZeKn0olhUe50y8LbuvJho-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=pZwg3cOxtPEB45-6ZJuKjpBvaR4hMvAION4xiM1-TXU&e= and include the link in your posting.

*****



Hi all,

Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful.

Thanks,

Niyanta



När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Hr finns information om hur KI behandlar personuppgifter<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=EQs1kTVGSWHn1Bbnwf8tsm9ZmeiX9rnpVF3Gx8njx2g&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=EQs1kTVGSWHn1Bbnwf8tsm9ZmeiX9rnpVF3Gx8njx2g&e=%20> >.


Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=3Bvbvriv0Qsm1rvTGdCTn69BFi2whwW0uFp_0iJEm9g&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=3Bvbvriv0Qsm1rvTGdCTn69BFi2whwW0uFp_0iJEm9g&e=%20> >.



När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.


Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.
Niyanta Kumar Niyanta Kumar
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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

In reply to this post by Niyanta Kumar
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks Sylvie, this is helpful!

Niyanta Kumar, Ph.D.
Senior Scientist - Pharmacokinetics and ADME
Merck Research Laboratories

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Sylvie Le Guyader
Sent: Wednesday, October 16, 2019 9:55 AM
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

EXTERNAL EMAIL – Use caution with any links or file attachments.

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’
Definitely! That would bore me too and I am not from the millennium generation! 😉
I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. 😊

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: +46 (0) 73 733 5008
LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility>
Follow our microscopy blog!<http://microscopykarolinska.se/>

From: Arvydas Matiukas <[hidden email]>
Sent: 16 October 2019 13:53
To: Sylvie Le Guyader <[hidden email]>; [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

Hello Sylvie,

Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.

with best wishes Arvydas




+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University

>>> Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>>
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*****

Hi Niyanta



Alyona Minina has tons of videos on you tube. Check her channel<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e=%20> >.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Niyanta Kumar
Sent: 11 October 2019 22:00
To: [hidden email]<mailto:[hidden email]>
Subject: Confocal and Widefield microscopy training material



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*****



Hi all,

Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful.

Thanks,

Niyanta



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Arvydas Matiukas Arvydas Matiukas
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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

In reply to this post by Sylvie Le Guyader
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Hi Silvie,

That's useful approach to try. Whenever possible I try an individual
approach matching the scope and depth of the material to the trainee's
background and skill level. The worst scenario is when a lab sends in
several trainees of different level (e.g. student, technician and
postdoc). Do you accept several trainees for the same session?
Next major difficulty  for the trainee that I noticed is the transition
from imaging training sample to the self-prepared one. Here the ability
to duplicate tutorial actions usually is not enough , in depth
understanding is required, and of course preparing quality samples.

Best regards,
Arvydas
>>> Sylvie Le Guyader <[hidden email]> 10/16/2019 9:54 AM >>>
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 and include the link in your posting.

*****

’ significant fraction of the basic confocal trainees bored after 1-2
hrs of total 3.’
Definitely! That would bore me too and I am not from the millennium
generation! ��
I believe that the only way is to make very short videos and give
quizzes half way or just afterwards. E.g. our trainee watches our video
about bit depth, saturation and under exposure knowing that right after
the video we will ask to show us where the buttons are and if there was
any saturation/under exposure in the image they took immediately before
watching the video. They are encouraged to stop the video and push the
buttons on the software whenever they feel like it. ��

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: +46 (0) 73 733 5008
LCI
website<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_bionut_welcome-2Dto-2Dthe-2Dlci-2Dfacility&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=XGh1Uu87TWxekpsyPOgE9NKlXFtrItrNJpVgKlIIhEk&s=eHHGZSUe6-p2KrVzjC_VHcLg3gh9nGLmDr6tud4_KTE&e=
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>


From: Arvydas Matiukas <[hidden email]>
Sent: 16 October 2019 13:53
To: Sylvie Le Guyader <[hidden email]>;
[hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training
material

Hello Sylvie,

Thanks for pointing to these video tutorials by Alyona Minina. They
nicely complement traditional powerpoint material and hopefully could
save some my time. I am curious to check how much longer video material
keeps trainee's attention focussed, especially for millenial youtube
generation. I noticed that traditional verbal and powerpoint
presentation makes significant fraction of the basic confocal trainees
bored after 1-2 hrs of total 3.

with best wishes Arvydas




+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University

>>> Sylvie Le Guyader
<[hidden email]<mailto:[hidden email]>> 10/16/19 6:28
AM >>>
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and include the link in your posting.

*****

Hi Niyanta



Alyona Minina has tons of videos on you tube. Check her
channel<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e=
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>.




Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List
<[hidden email]<mailto:[hidden email]>>
On Behalf Of Niyanta Kumar
Sent: 11 October 2019 22:00
To:
[hidden email]<mailto:[hidden email]>
Subject: Confocal and Widefield microscopy training material



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and include the link in your posting.


*****



Hi all,

Can someone suggest any existing resources and formats to pull from for
designing new and advanced user trainings for confocal and widefield
microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2
confocal and an Axio Obsever widefield scope. Interactive resources or
existing quizzes that can ensure users know what they need to would also
be helpful.

Thanks,

Niyanta



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KI kommer att behandla dina personuppgifter. Hr finns information om hur
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Sylvie Le Guyader Sylvie Le Guyader
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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

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Hi again



We never use any training sample. The reason why our training is so long (and expensive: 1200€) is because we troubleshoot the sample preparation and experimental design extensively before and during (and after) the training.

After our first 1h meeting briefing the researcher on how to modify their sample if it is needed (most of the time but not always), we demand that they bring the correct samples during the training.

It is very rare that they don’t and come up with samples that they got from a colleague. If they do we cancel the training and send them back to the bench.

Most of them understand after our 1h meeting that it is fully in their interest to get the best samples possible during the training because we help them acquire data during the week.



Our trainings are mandatory 1 to 1. We do not train people together. Therefore the training is always adapted to their sample/experiment/experience/personality...

My colleague Gabriela Imreh does all the trainings. She squeezes about 40 trainings per year outside of holidays. And our microscopy course takes 1 full month during which there is no training.



Keeps us seriously interested and busy! 😉



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Arvydas Matiukas
Sent: 16 October 2019 17:46
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material



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Hi Silvie,



That's useful approach to try. Whenever possible I try an individual approach matching the scope and depth of the material to the trainee's background and skill level. The worst scenario is when a lab sends in several trainees of different level (e.g. student, technician and postdoc). Do you accept several trainees for the same session?

Next major difficulty  for the trainee that I noticed is the transition from imaging training sample to the self-prepared one. Here the ability to duplicate tutorial actions usually is not enough , in depth understanding is required, and of course preparing quality samples.



Best regards,

Arvydas

>>> Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/2019 9:54 AM >>>

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’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’

Definitely! That would bore me too and I am not from the millennium generation!    I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI

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From: Arvydas Matiukas <[hidden email]<mailto:[hidden email]>>

Sent: 16 October 2019 13:53

To: Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>>; [hidden email]<mailto:[hidden email]>

Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material



Hello Sylvie,



Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.



with best wishes Arvydas









+++++++++++++++++++++++++++++

Arvydas Matiukas, Ph.D.

Manager of NRB Shared Equipment

Director of Confocal&Two-Photon Core

SUNY Upstate Medical University



>>> Sylvie Le Guyader

<[hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>> 10/16/19 6:28 AM >>>

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*****



Hi Niyanta







Alyona Minina has tons of videos on you tube. Check her channel<https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DfqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8%26s%3Do-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE%26e&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C4b1fe493ee6a493a196208d752500e40%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068376112645232&amp;sdata=xxysD6Y0SDBta3iOTOKHowWhdv8%2Bfmhc7kZNa9iN7YE%3D&amp;reserved=0=

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Med vänlig hälsning / Best regards







Sylvie







@@@@@@@@@@@@@@@@@@@@@@@@



Sylvie Le Guyader, PhD



Live Cell Imaging Facility Manager



Karolinska Institutet- Bionut Dpt



Blickagången 16,



Room 7362 (lab)/7840 (office)



14157 Huddinge, Sweden



mobile: +46 (0) 73 733 5008



LCI website



Follow our microscopy blog!







-----Original Message-----

From: Confocal Microscopy List

<[hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>>

On Behalf Of Niyanta Kumar

Sent: 11 October 2019 22:00

To:

[hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>

Subject: Confocal and Widefield microscopy training material







*****



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*****







Hi all,



Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful.



Thanks,



Niyanta







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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

In reply to this post by Arvydas Matiukas
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The discussions on microscopy training are extremely valuable.  I need to review all the replies again and, based on them, revise the rules I posted, modify how we do training, and write directions for new users to read before training.

In response to Arvydas's question about trainees, I find that status as student, postdoc, technician, or PI has no bearing on existing expertise or willingness or ability to learn the material.  Why or how people learn diverge widely based on many factors.

Small groups of trainees is good for saving time on the material that can be shown and discussed, however, only the person who gets to drive the machine may be ready to use it unassisted.  Everyone else needs to follow up with practical training.

Cheers-

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
Office: 646-501-0567 Cell: 914-309-3270  [hidden email]  
http://nyulmc.org/micros  http://microscopynotes.com/
 



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Arvydas Matiukas
Sent: Wednesday, October 16, 2019 11:46 AM
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material


Hi Silvie,

That's useful approach to try. Whenever possible I try an individual approach matching the scope and depth of the material to the trainee's background and skill level. The worst scenario is when a lab sends in several trainees of different level (e.g. student, technician and postdoc). Do you accept several trainees for the same session?
Next major difficulty  for the trainee that I noticed is the transition from imaging training sample to the self-prepared one. Here the ability to duplicate tutorial actions usually is not enough , in depth understanding is required, and of course preparing quality samples.

Best regards,
Arvydas
>>> Sylvie Le Guyader <[hidden email]> 10/16/2019 9:54 AM >>>
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’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’
Definitely! That would bore me too and I am not from the millennium generation!    I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it.  

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
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mobile: +46 (0) 73 733 5008
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From: Arvydas Matiukas <[hidden email]>
Sent: 16 October 2019 13:53
To: Sylvie Le Guyader <[hidden email]>; [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

Hello Sylvie,

Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.

with best wishes Arvydas




+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University

>>> Sylvie Le Guyader
<[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>>
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*****

Hi Niyanta



Alyona Minina has tons of videos on you tube. Check her channel<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e=
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Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List
<[hidden email]<mailto:[hidden email]>>
On Behalf Of Niyanta Kumar
Sent: 11 October 2019 22:00
To:
[hidden email]<mailto:[hidden email]>
Subject: Confocal and Widefield microscopy training material



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Hi all,

Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful.

Thanks,

Niyanta



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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

In reply to this post by Sylvie Le Guyader
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Hi Silvie,

Thanks for providing whole outline of your training. Now I see that I
and some other Core Managers were comparing basic (confocal) microscopy
training aimed at self-service usage of Core equipment vs experimental
part of the  Microscopy Course/workshop. I think both types of training
are useful and have their advantages. Like not everybody is aiming at or
above PhD.

>90% of my Core users are new lab members that just continue following
their lab protocols and require only instruction how to use (and don't
break - it is really critical because messes up plans of multiple labs)
Core microscopes. If I would advice/pressure them on Silvie's approach
they would bring their samples and pay me $1200 for imaging/analysis .
Their "rock solid" argument is "if other Cores can train in 3 hrs why
Confocal can't, then provide full service". My experience with over 400
trainees is that it takes 4-10 hrs of theoretical and practical training
one on one to provide the skill of unassisted confocal operation.  And
only <10% that need to develop new protocol/application agree to the
full featured project consultation/assistance . However, such
collaborations usually take years not a month.

I guess Cores may use different approaches and still make their users
happy. Anyway it is very useful to exchange various training
approaches.

  For Niyanta I would mention iBiology Microscope Course available on
their website or Youtube.

Best regards,
Arvydas
>>> Sylvie Le Guyader <[hidden email]> 10/16/2019 12:34 PM
>>>
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Hi again



We never use any training sample. The reason why our training is so
long (and expensive: 1200€) is because we troubleshoot the sample
preparation and experimental design extensively before and during (and
after) the training.

After our first 1h meeting briefing the researcher on how to modify
their sample if it is needed (most of the time but not always), we
demand that they bring the correct samples during the training.

It is very rare that they don’t and come up with samples that they got
from a colleague. If they do we cancel the training and send them back
to the bench.

Most of them understand after our 1h meeting that it is fully in their
interest to get the best samples possible during the training because we
help them acquire data during the week.



Our trainings are mandatory 1 to 1. We do not train people together.
Therefore the training is always adapted to their
sample/experiment/experience/personality...

My colleague Gabriela Imreh does all the trainings. She squeezes about
40 trainings per year outside of holidays. And our microscopy course
takes 1 full month during which there is no training.



Keeps us seriously interested and busy! ��



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On
Behalf Of Arvydas Matiukas
Sent: 16 October 2019 17:46
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training
material



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*****



Hi Silvie,



That's useful approach to try. Whenever possible I try an individual
approach matching the scope and depth of the material to the trainee's
background and skill level. The worst scenario is when a lab sends in
several trainees of different level (e.g. student, technician and
postdoc). Do you accept several trainees for the same session?

Next major difficulty  for the trainee that I noticed is the transition
from imaging training sample to the self-prepared one. Here the ability
to duplicate tutorial actions usually is not enough , in depth
understanding is required, and of course preparing quality samples.



Best regards,

Arvydas

>>> Sylvie Le Guyader
<[hidden email]<mailto:[hidden email]>> 10/16/2019
9:54 AM >>>

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and include the link in your posting.



*****



’ significant fraction of the basic confocal trainees bored after 1-2
hrs of total 3.’

Definitely! That would bore me too and I am not from the millennium
generation!    I believe that the only way is to make very short videos
and give quizzes half way or just afterwards. E.g. our trainee watches
our video about bit depth, saturation and under exposure knowing that
right after the video we will ask to show us where the buttons are and
if there was any saturation/under exposure in the image they took
immediately before watching the video. They are encouraged to stop the
video and push the buttons on the software whenever they feel like it.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI

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>



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>





From: Arvydas Matiukas
<[hidden email]<mailto:[hidden email]>>

Sent: 16 October 2019 13:53

To: Sylvie Le Guyader
<[hidden email]<mailto:[hidden email]>>;
[hidden email]<mailto:[hidden email]>

Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training
material



Hello Sylvie,



Thanks for pointing to these video tutorials by Alyona Minina. They
nicely complement traditional powerpoint material and hopefully could
save some my time. I am curious to check how much longer video material
keeps trainee's attention focussed, especially for millenial youtube
generation. I noticed that traditional verbal and powerpoint
presentation makes significant fraction of the basic confocal trainees
bored after 1-2 hrs of total 3.



with best wishes Arvydas









+++++++++++++++++++++++++++++

Arvydas Matiukas, Ph.D.

Manager of NRB Shared Equipment

Director of Confocal&Two-Photon Core

SUNY Upstate Medical University



>>> Sylvie Le Guyader

<[hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>>
10/16/19 6:28 AM >>>

*****

To join, leave or search the confocal microscopy listserv, go to:

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*****



Hi Niyanta







Alyona Minina has tons of videos on you tube. Check her
channel<https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttps-2D3A-5F-5Fwww.youtube.com-5Fchannel-5FUCfRx0TPF-2D2DR1TKYrPPu7OwWA-5Fvideos-2526d-253DDwIGaQ-2526c-253Dogn2iPkgF7TkVSicOVBfKg-2526r-253DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-2D4k-2526m-253DfqPKyH7OvDC2YG-2DCgHu2VMvZCDwOwF5ALyEnXO-2DYnB8-2526s-253Do-2DJPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE-2526e-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C4b1fe493ee6a493a196208d752500e40-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637068376112645232-26amp-3Bsdata-3DxxysD6Y0SDBta3iOTOKHowWhdv8-252Bfmhc7kZNa9iN7YE-253D-26amp-3Breserved-3D0-3D&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=c-c2p2VyKvCQavJtsgSHq1tAhcVdrsuALsZgaBE-kP0&s=d63j6F6xl2DZ4c7L4ng53UK2ne2z-qAwxLcNx7ia66M&e=



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>


>.









Med vänlig hälsning / Best regards







Sylvie







@@@@@@@@@@@@@@@@@@@@@@@@



Sylvie Le Guyader, PhD



Live Cell Imaging Facility Manager



Karolinska Institutet- Bionut Dpt



Blickagången 16,



Room 7362 (lab)/7840 (office)



14157 Huddinge, Sweden



mobile: +46 (0) 73 733 5008



LCI website



Follow our microscopy blog!







-----Original Message-----

From: Confocal Microscopy List

<[hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>>

On Behalf Of Niyanta Kumar

Sent: 11 October 2019 22:00

To:

[hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>

Subject: Confocal and Widefield microscopy training material







*****



To join, leave or search the confocal microscopy listserv, go to:



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and include the link in your posting.





*****







Hi all,



Can someone suggest any existing resources and formats to pull from for
designing new and advanced user trainings for confocal and widefield
microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2
confocal and an Axio Obsever widefield scope. Interactive resources or
existing quizzes that can ensure users know what they need to would also
be helpful.



Thanks,



Niyanta







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Jacqueline Ross Jacqueline Ross
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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

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Hi everyone,

We train people in the same way as Sylvie as I have mentioned on this list previously. This works not only to make sure people are well trained and supported but also to take care of the equipment.

I also want to mention the great MyScope resource hosted by the University of Sydney from Microscopy Australia. You can find it here: https://myscope.training/index.html

Cheers,

Jacqui

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Arvydas Matiukas
Sent: Thursday, 17 October 2019 8:39 a.m.
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

*****
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Hi Silvie,

Thanks for providing whole outline of your training. Now I see that I
and some other Core Managers were comparing basic (confocal) microscopy
training aimed at self-service usage of Core equipment vs experimental
part of the  Microscopy Course/workshop. I think both types of training
are useful and have their advantages. Like not everybody is aiming at or
above PhD.

>90% of my Core users are new lab members that just continue following
their lab protocols and require only instruction how to use (and don't
break - it is really critical because messes up plans of multiple labs)
Core microscopes. If I would advice/pressure them on Silvie's approach
they would bring their samples and pay me $1200 for imaging/analysis .
Their "rock solid" argument is "if other Cores can train in 3 hrs why
Confocal can't, then provide full service". My experience with over 400
trainees is that it takes 4-10 hrs of theoretical and practical training
one on one to provide the skill of unassisted confocal operation.  And
only <10% that need to develop new protocol/application agree to the
full featured project consultation/assistance . However, such
collaborations usually take years not a month.

I guess Cores may use different approaches and still make their users
happy. Anyway it is very useful to exchange various training
approaches.

  For Niyanta I would mention iBiology Microscope Course available on
their website or Youtube.

Best regards,
Arvydas
>>> Sylvie Le Guyader <[hidden email]> 10/16/2019 12:34 PM
>>>
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Hi again



We never use any training sample. The reason why our training is so
long (and expensive: 1200€) is because we troubleshoot the sample
preparation and experimental design extensively before and during (and
after) the training.

After our first 1h meeting briefing the researcher on how to modify
their sample if it is needed (most of the time but not always), we
demand that they bring the correct samples during the training.

It is very rare that they don’t and come up with samples that they got
from a colleague. If they do we cancel the training and send them back
to the bench.

Most of them understand after our 1h meeting that it is fully in their
interest to get the best samples possible during the training because we
help them acquire data during the week.



Our trainings are mandatory 1 to 1. We do not train people together.
Therefore the training is always adapted to their
sample/experiment/experience/personality...

My colleague Gabriela Imreh does all the trainings. She squeezes about
40 trainings per year outside of holidays. And our microscopy course
takes 1 full month during which there is no training.



Keeps us seriously interested and busy! ��



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On
Behalf Of Arvydas Matiukas
Sent: 16 October 2019 17:46
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training
material



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*****



Hi Silvie,



That's useful approach to try. Whenever possible I try an individual
approach matching the scope and depth of the material to the trainee's
background and skill level. The worst scenario is when a lab sends in
several trainees of different level (e.g. student, technician and
postdoc). Do you accept several trainees for the same session?

Next major difficulty  for the trainee that I noticed is the transition
from imaging training sample to the self-prepared one. Here the ability
to duplicate tutorial actions usually is not enough , in depth
understanding is required, and of course preparing quality samples.



Best regards,

Arvydas

>>> Sylvie Le Guyader
<[hidden email]<mailto:[hidden email]>> 10/16/2019
9:54 AM >>>

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*****



’ significant fraction of the basic confocal trainees bored after 1-2
hrs of total 3.’

Definitely! That would bore me too and I am not from the millennium
generation!    I believe that the only way is to make very short videos
and give quizzes half way or just afterwards. E.g. our trainee watches
our video about bit depth, saturation and under exposure knowing that
right after the video we will ask to show us where the buttons are and
if there was any saturation/under exposure in the image they took
immediately before watching the video. They are encouraged to stop the
video and push the buttons on the software whenever they feel like it.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

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From: Arvydas Matiukas
<[hidden email]<mailto:[hidden email]>>

Sent: 16 October 2019 13:53

To: Sylvie Le Guyader
<[hidden email]<mailto:[hidden email]>>;
[hidden email]<mailto:[hidden email]>

Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training
material



Hello Sylvie,



Thanks for pointing to these video tutorials by Alyona Minina. They
nicely complement traditional powerpoint material and hopefully could
save some my time. I am curious to check how much longer video material
keeps trainee's attention focussed, especially for millenial youtube
generation. I noticed that traditional verbal and powerpoint
presentation makes significant fraction of the basic confocal trainees
bored after 1-2 hrs of total 3.



with best wishes Arvydas









+++++++++++++++++++++++++++++

Arvydas Matiukas, Ph.D.

Manager of NRB Shared Equipment

Director of Confocal&Two-Photon Core

SUNY Upstate Medical University



>>> Sylvie Le Guyader

<[hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>>
10/16/19 6:28 AM >>>

*****

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and include the link in your posting.



*****



Hi Niyanta







Alyona Minina has tons of videos on you tube. Check her
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>


>.









Med vänlig hälsning / Best regards







Sylvie







@@@@@@@@@@@@@@@@@@@@@@@@



Sylvie Le Guyader, PhD



Live Cell Imaging Facility Manager



Karolinska Institutet- Bionut Dpt



Blickagången 16,



Room 7362 (lab)/7840 (office)



14157 Huddinge, Sweden



mobile: +46 (0) 73 733 5008



LCI website



Follow our microscopy blog!







-----Original Message-----

From: Confocal Microscopy List

<[hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>>

On Behalf Of Niyanta Kumar

Sent: 11 October 2019 22:00

To:

[hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>

Subject: Confocal and Widefield microscopy training material







*****



To join, leave or search the confocal microscopy listserv, go to:



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and include the link in your posting.





*****







Hi all,



Can someone suggest any existing resources and formats to pull from for
designing new and advanced user trainings for confocal and widefield
microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2
confocal and an Axio Obsever widefield scope. Interactive resources or
existing quizzes that can ensure users know what they need to would also
be helpful.



Thanks,



Niyanta







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Neftali Flores Rodriguez Neftali Flores Rodriguez
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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

In reply to this post by Niyanta Kumar
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Hi Niyanta,

You might find our MyScope useful.

https://myscope.training/


Neftali Flores-Rodriguez | Light Microscopist

Sydney Microscopy and Microanalysis (SMM)
Australian Centre for Microscopy & Microanalysis (ACMM)

THE UNIVERSITY OF SYDNEY


________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Kumar, Niyanta <[hidden email]>
Sent: Wednesday, 16 October 2019, 6:04 pm
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

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Thanks Sylvie, this is helpful!

Niyanta Kumar, Ph.D.
Senior Scientist - Pharmacokinetics and ADME
Merck Research Laboratories

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Sylvie Le Guyader
Sent: Wednesday, October 16, 2019 9:55 AM
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

EXTERNAL EMAIL – Use caution with any links or file attachments.

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’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’
Definitely! That would bore me too and I am not from the millennium generation! 😉
I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. 😊

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: +46 (0) 73 733 5008
LCI website<https://protect-au.mimecast.com/s/40xyCMwvLQTAQG37hJE2HF?domain=ki.se>
Follow our microscopy blog!<https://protect-au.mimecast.com/s/vt_4CNLwM9iG213zIRmKQP?domain=microscopykarolinska.se>

From: Arvydas Matiukas <[hidden email]>
Sent: 16 October 2019 13:53
To: Sylvie Le Guyader <[hidden email]>; [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

Hello Sylvie,

Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.

with best wishes Arvydas




+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University

>>> Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>>
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*****

Hi Niyanta



Alyona Minina has tons of videos on you tube. Check her channel<https://protect-au.mimecast.com/s/Dn7vCQnzP0ty5mPYc99xjB?domain=urldefense.proofpoint.com <https://protect-au.mimecast.com/s/dOszCROAQotzA8WxT05sni?domain=urldefense.proofpoint.com> >.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Niyanta Kumar
Sent: 11 October 2019 22:00
To: [hidden email]<mailto:[hidden email]>
Subject: Confocal and Widefield microscopy training material



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Hi all,

Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful.

Thanks,

Niyanta



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Katherina Garcia Garcia Katherina Garcia Garcia
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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

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Hello,

https://www.ibiology.org 

It is wonderful. It has a microscopy course with more than 70 talks
given by experts in the field, including Nobel Prize winners.

Enjoy it!

Kathy

El 2019-10-17 06:13, Neftali Flores Rodriguez escribió:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Niyanta,
>
> You might find our MyScope useful.
>
> https://myscope.training/
>
> Neftali Flores-Rodriguez | Light Microscopist
>
> Sydney Microscopy and Microanalysis (SMM)
> Australian Centre for Microscopy & Microanalysis (ACMM)
>
> THE UNIVERSITY OF SYDNEY
>
> ________________________________
> From: Confocal Microscopy List <[hidden email]> on behalf of Kumar, Niyanta <[hidden email]>
> Sent: Wednesday, 16 October 2019, 6:04 pm
> To: [hidden email]
> Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu
> Post images on https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com and include the link in your posting.
> *****
>
> Thanks Sylvie, this is helpful!
>
> Niyanta Kumar, Ph.D.
> Senior Scientist - Pharmacokinetics and ADME
> Merck Research Laboratories
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On Behalf Of Sylvie Le Guyader
> Sent: Wednesday, October 16, 2019 9:55 AM
> To: [hidden email]
> Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
>
> EXTERNAL EMAIL - Use caution with any links or file attachments.
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu
> Post images on https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com and include the link in your posting.
> *****
>
> ' significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.'
> Definitely! That would bore me too and I am not from the millennium generation! 😉
> I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. 😊
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Blickagången 16,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: +46 (0) 73 733 5008
> LCI website<https://protect-au.mimecast.com/s/40xyCMwvLQTAQG37hJE2HF?domain=ki.se>
> Follow our microscopy blog!<https://protect-au.mimecast.com/s/vt_4CNLwM9iG213zIRmKQP?domain=microscopykarolinska.se>
>
> From: Arvydas Matiukas <[hidden email]>
> Sent: 16 October 2019 13:53
> To: Sylvie Le Guyader <[hidden email]>; [hidden email]
> Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
>
> Hello Sylvie,
>
> Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.
>
> with best wishes Arvydas
>
> +++++++++++++++++++++++++++++
> Arvydas Matiukas, Ph.D.
> Manager of NRB Shared Equipment
> Director of Confocal&Two-Photon Core
> SUNY Upstate Medical University
>
> Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>>
 *****
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*****

Hi Niyanta

Alyona Minina has tons of videos on you tube. Check her
channel<https://protect-au.mimecast.com/s/Dn7vCQnzP0ty5mPYc99xjB?domain=urldefense.proofpoint.com
<https://protect-au.mimecast.com/s/dOszCROAQotzA8WxT05sni?domain=urldefense.proofpoint.com>
>.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!

-----Original Message-----
From: Confocal Microscopy List
<[hidden email]<mailto:[hidden email]>>
On Behalf Of Niyanta Kumar
Sent: 11 October 2019 22:00
To:
[hidden email]<mailto:[hidden email]>
Subject: Confocal and Widefield microscopy training material

*****

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and include the link in your posting.

*****

Hi all,

Can someone suggest any existing resources and formats to pull from for
designing new and advanced user trainings for confocal and widefield
microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2
confocal and an Axio Obsever widefield scope. Interactive resources or
existing quizzes that can ensure users know what they need to would also
be helpful.

Thanks,

Niyanta

När du skickar e-post till Karolinska Institutet (KI) innebär detta att
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KI behandlar
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<https://protect-au.mimecast.com/s/XRVlCYWL1vi4zPlwurPdw1?domain=urldefense.proofpoint.com>
>.

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>.

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Steffen Dietzel Steffen Dietzel
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Re: Confocal and Widefield microscopy training material

In reply to this post by Niyanta Kumar
*****
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Hi Niyanta,

I have collected a few resources on our website, so that I can refer our
users to it more easily. This is at
https://www.bioimaging.bmc.med.uni-muenchen.de/learn/
Have a look at "Educational Websites" and "Materials for teaching".
Maybe you find something helpful. The listed page from Australia (was
mentioned by others too) contains a quiz that you can take, and you can
print out a certificate if you answer 8+ of 10 questions correct.

As in terms of hands on training, we start with a 3 h introduction
session for up to 3 people. Usually with our samples, for two reasons.
First, we want the user to pay attention to the microscope and software,
not on the biological content of the images we make. Second, this way we
can be sure that the sample is all right and we don't have users with
crappy samples blaming it on the microscope. I agree with others that
after 3 hours it makes no more sense, brains are full.

Next we do a 'guided session' with the user's sample that we accompany
until the user is getting good results. This is 1:1. Depending on sample
difficulty, prior knowledge and user talent we sometimes have a third
session.

I am impressed by Sylvie's approach, but I doubt our group leaders would
be willing to come up with so much money for training. Some think what
we do now is already too time consuming (but most are happy about it).


Steffen

Am 11.10.2019 um 21:59 schrieb Niyanta Kumar:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all,
> Can someone suggest any existing resources and formats to pull from
> for designing new and advanced user trainings for confocal and
> widefield microscopy? I need to design trainings for a Zeiss LSM 980
> Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive
> resources or existing quizzes that can ensure users know what they
> need to would also be helpful.
> Thanks,
> Niyanta
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de
Sylvie Le Guyader Sylvie Le Guyader
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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

In reply to this post by Katherina Garcia Garcia
*****
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*****

Jennifer Waters microscopy microcourses on Youtube. Short and excellent.
https://www.youtube.com/channel/UC4cOKa0TZK8CQhzSQqfwuMQ

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: +46 (0) 73 733 5008
LCI website
Follow our microscopy blog!

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Katherina Garcia Garcia
Sent: 17 October 2019 09:46
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

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Hello,

https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ibiology.org&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&amp;sdata=Rwt5ADH9k1ytvIUh3PA4VOPFowJ6a8HWz%2BZhwVR4IOU%3D&amp;reserved=0

It is wonderful. It has a microscopy course with more than 70 talks given by experts in the field, including Nobel Prize winners.

Enjoy it!

Kathy

El 2019-10-17 06:13, Neftali Flores Rodriguez escribió:

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> *****
>
> Hi Niyanta,
>
> You might find our MyScope useful.
>
> https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fmysc
> ope.training%2F&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd5
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> lUQg0%3D&amp;reserved=0
>
> Neftali Flores-Rodriguez | Light Microscopist
>
> Sydney Microscopy and Microanalysis (SMM) Australian Centre for
> Microscopy & Microanalysis (ACMM)
>
> THE UNIVERSITY OF SYDNEY
>
> ________________________________
> From: Confocal Microscopy List <[hidden email]> on
> behalf of Kumar, Niyanta
> <[hidden email]>
> Sent: Wednesday, 16 October 2019, 6:04 pm
> To: [hidden email]
> Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training
> material
>
> *****
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> *****
>
> Thanks Sylvie, this is helpful!
>
> Niyanta Kumar, Ph.D.
> Senior Scientist - Pharmacokinetics and ADME Merck Research
> Laboratories
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Sylvie Le Guyader
> Sent: Wednesday, October 16, 2019 9:55 AM
> To: [hidden email]
> Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training
> material
>
> EXTERNAL EMAIL - Use caution with any links or file attachments.
>
> *****
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> *****
>
> ' significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.'
> Definitely! That would bore me too and I am not from the millennium
> generation! 😉
> I believe that the only way is to make very short videos and give
> quizzes half way or just afterwards. E.g. our trainee watches our
> video about bit depth, saturation and under exposure knowing that
> right after the video we will ask to show us where the buttons are and
> if there was any saturation/under exposure in the image they took
> immediately before watching the video. They are encouraged to stop the
> video and push the buttons on the software whenever they feel like it.
> 😊
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Blickagången 16,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: +46 (0) 73 733 5008
> LCI
> website<<a href="https://eur01.safelinks.protection.outlook.com/?url=https%3A%2">https://eur01.safelinks.protection.outlook.com/?url=https%3A%2
> F%2Fprotect-au.mimecast.com%2Fs%2F40xyCMwvLQTAQG37hJE2HF%3Fdomain%3Dki
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> 1018805&amp;sdata=SiFrrYL3Ao772BTc%2BoI7CfzSVsqO52FoLJPLGWzXj8A%3D&amp
> ;reserved=0> Follow our microscopy
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> MMcLToMGCUA%3D&amp;reserved=0>
>
> From: Arvydas Matiukas <[hidden email]>
> Sent: 16 October 2019 13:53
> To: Sylvie Le Guyader <[hidden email]>;
> [hidden email]
> Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training
> material
>
> Hello Sylvie,
>
> Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.
>
> with best wishes Arvydas
>
> +++++++++++++++++++++++++++++
> Arvydas Matiukas, Ph.D.
> Manager of NRB Shared Equipment
> Director of Confocal&Two-Photon Core
> SUNY Upstate Medical University
>
> Sylvie Le Guyader
> <[hidden email]<mailto:[hidden email]>> 10/16/19
> 6:28 AM >>>
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Hi Niyanta

Alyona Minina has tons of videos on you tube. Check her
channel<https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprotect-au.mimecast.com%2Fs%2FDn7vCQnzP0ty5mPYc99xjB%3Fdomain%3Durldefense.proofpoint.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&amp;sdata=4b0w7we4ofQ9aOSshTcsztwXWH4UXGice2Z%2BqQL%2FAMs%3D&amp;reserved=0
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>.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Blickagången 16,

Room 7362 (lab)/7840 (office)

14157 Huddinge, Sweden

mobile: +46 (0) 73 733 5008

LCI website

Follow our microscopy blog!

-----Original Message-----
From: Confocal Microscopy List
<[hidden email]<mailto:[hidden email]>>
On Behalf Of Niyanta Kumar
Sent: 11 October 2019 22:00
To:
[hidden email]<mailto:[hidden email]>
Subject: Confocal and Widefield microscopy training material

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Hi all,

Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful.

Thanks,

Niyanta

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Arvydas Matiukas Arvydas Matiukas
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Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material

In reply to this post by Steffen Dietzel
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Hi Steffen, Silvie

Thanks for very interesting  and useful discussion. Few  summary
thoughts:

1. I second Steffens' reasoning for starting training with Core
provided=known=calibrated samples. Sylvie's approach of starting with
users'=non calibrated sample introduces to many unknowns at a time and
to my opinion reduces efficiency of the training. I personally prefer
not to be trained in such stressful way.

2. I wonder if many  labs in  EU can afford to spend 1 month of their
member time  and pay 1200EUR to get the members trained in advanced
level microscopy. In the USA it definitely not going to happen on any
significant scale.
Maybe a cultural difference :) I recall the comparison provided by US
based Zeiss specialist after his training in Germany: "problem=
ballpoint pen stopped writing. German style solution = research
potential causes, i.e. disassemble the pen, check if any ink is left, if
ball rotates freely, finally if the user properly presses knob or
rotates the body. This approach may take significant but reasonable time
and provide useful skills and experience for the future. American style
solution = check the pen price first. As it is much lower than the fee
of professional spending  hours on this problem then just trash the pen
and get a new one"

3. Of course in the USA there are labs that need advanced level
microscopy. However, typically they have their own microscopes and
microscopy experts and very rarely use Core services.
Among my 400 trainees over 15 years only about 10 expressed willingness
to proceed to the advanced level, and among them only 1 grad student.

4. Finally, time and cost considerations. At 1 month and 1200EUR
spending level how many techniques a beginner researcher may learn in  
1 year ? 3years? BTW I think training in optical/confocal microscopy is
relatively inexpensive, preparing your own samples typically is more
time consuming and expensive, and learning other tecniques may be more
expensive as well.

My 2 cents,
Arvydas

+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Neuroscience & Physiology Dept



>>> Steffen Dietzel <[hidden email]> 10/17/2019 6:55 AM >>>
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Post images on
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=yAGFeShn7KSa-eW2mPu6XbhS9SeX7BD0-L0UF5cyjgE&e=
 and include the link in your posting.

*****

Hi Niyanta,

I have collected a few resources on our website, so that I can refer
our
users to it more easily. This is at
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_learn_&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=Z2jynrNg35the3m1H5X3AsUA52lnDqOb4qZUaoY05dY&e=


Have a look at "Educational Websites" and "Materials for teaching".
Maybe you find something helpful. The listed page from Australia (was
mentioned by others too) contains a quiz that you can take, and you can

print out a certificate if you answer 8+ of 10 questions correct.

As in terms of hands on training, we start with a 3 h introduction
session for up to 3 people. Usually with our samples, for two reasons.

First, we want the user to pay attention to the microscope and
software,
not on the biological content of the images we make. Second, this way
we
can be sure that the sample is all right and we don't have users with
crappy samples blaming it on the microscope. I agree with others that
after 3 hours it makes no more sense, brains are full.

Next we do a 'guided session' with the user's sample that we accompany

until the user is getting good results. This is 1:1. Depending on
sample
difficulty, prior knowledge and user talent we sometimes have a third
session.

I am impressed by Sylvie's approach, but I doubt our group leaders
would
be willing to come up with so much money for training. Some think what

we do now is already too time consuming (but most are happy about it).


Steffen

Am 11.10.2019 um 21:59 schrieb Niyanta Kumar:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=k0lBHjfBdP6gkIAbpZx0fMuOqb1oDSvuwF7Hk7nelgY&e=


> Post images on
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=yAGFeShn7KSa-eW2mPu6XbhS9SeX7BD0-L0UF5cyjgE&e=
 and include the link in your posting.

> *****
>
> Hi all,
> Can someone suggest any existing resources and formats to pull from
> for designing new and advanced user trainings for confocal and
> widefield microscopy? I need to design trainings for a Zeiss LSM 980

> Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive

> resources or existing quizzes that can ensure users know what they
> need to would also be helpful.
> Thanks,
> Niyanta
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität Mnchen
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

https://urldefense.proofpoint.com/v2/url?u=http-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=Gadulq06lK7kYY94plB3DsipjiGMo01xTiPc_poN3_Y&e=
Niyanta Kumar Niyanta Kumar
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Re: Confocal and Widefield microscopy training material

In reply to this post by Niyanta Kumar
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel
Sent: Thursday, October 17, 2019 6:55 AM
To: [hidden email]
Subject: Re: Confocal and Widefield microscopy training material

EXTERNAL EMAIL – Use caution with any links or file attachments.

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Niyanta,

I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://www.bioimaging.bmc.med.uni-muenchen.de/learn/
Have a look at "Educational Websites" and "Materials for teaching".
Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct.

As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons.
First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full.

Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session.

I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it).


Steffen

Am 11.10.2019 um 21:59 schrieb Niyanta Kumar:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all,
> Can someone suggest any existing resources and formats to pull from
> for designing new and advanced user trainings for confocal and
> widefield microscopy? I need to design trainings for a Zeiss LSM 980
> Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive
> resources or existing quizzes that can ensure users know what they
> need to would also be helpful.
> Thanks,
> Niyanta
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.

Phillips, Thomas E. Phillips, Thomas E.
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Re: Confocal and Widefield microscopy training material

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Does anybody offer their confocal training as an official grad-level class? In the past, offering a hands-on class was problematic for us since (i) no tuition dollars flow back to the core and (ii) PI's wanted their students using their own samples so "free class time" meant no income for us when those projects would normally have been done outside of class. Our campus is moving to a new budget model and tuition dollars may flow more directly back to us. We are considering combining training and supervised independent use as part of the class design and allow student efforts to be credentialed. I would be interested to know if anyone else is taking this approach and how many credit hours you offer and what you cover as part of the course.

I have never met a PI who would have paid for Sylvie's design and impressed her clients do so.



Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Curators’ Distinguished Teaching Professor
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
[hidden email]

http://www.biology.missouri.edu/faculty/phillips.html
http://microscopy.missouri.edu 

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Kumar, Niyanta
Sent: Thursday, October 17, 2019 2:12 PM
To: [hidden email]
Subject: Re: Confocal and Widefield microscopy training material

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel
Sent: Thursday, October 17, 2019 6:55 AM
To: [hidden email]
Subject: Re: Confocal and Widefield microscopy training material

EXTERNAL EMAIL – Use caution with any links or file attachments.

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Niyanta,

I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://www.bioimaging.bmc.med.uni-muenchen.de/learn/
Have a look at "Educational Websites" and "Materials for teaching".
Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct.

As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons.
First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full.

Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session.

I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it).


Steffen

Am 11.10.2019 um 21:59 schrieb Niyanta Kumar:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all,
> Can someone suggest any existing resources and formats to pull from
> for designing new and advanced user trainings for confocal and
> widefield microscopy? I need to design trainings for a Zeiss LSM 980
> Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive
> resources or existing quizzes that can ensure users know what they
> need to would also be helpful.
> Thanks,
> Niyanta
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de
Notice:  This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.

Craig Brideau Craig Brideau
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Re: Confocal and Widefield microscopy training material

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Here at University of Calgary we offer a Basic and an Advanced microscopy
course, each taking a full semester. The Advanced class covers confocal and
related techniques. We have had very good outcomes with graduate students
who have completed these courses.

Craig

On Thu, Oct 17, 2019 at 1:34 PM Phillips, Thomas E. <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Does anybody offer their confocal training as an official grad-level
> class? In the past, offering a hands-on class was problematic for us since
> (i) no tuition dollars flow back to the core and (ii) PI's wanted their
> students using their own samples so "free class time" meant no income for
> us when those projects would normally have been done outside of class. Our
> campus is moving to a new budget model and tuition dollars may flow more
> directly back to us. We are considering combining training and supervised
> independent use as part of the class design and allow student efforts to be
> credentialed. I would be interested to know if anyone else is taking this
> approach and how many credit hours you offer and what you cover as part of
> the course.
>
> I have never met a PI who would have paid for Sylvie's design and
> impressed her clients do so.
>
>
>
> Thomas E. Phillips, Ph.D
> Professor of Biological Sciences
> Curators’ Distinguished Teaching Professor
> Director, Molecular Cytology Core
> 2 Tucker Hall
> University of Missouri
> Columbia, MO 65211-7400
> 573-882-4712 (office)
> 573-882-0123 (fax)
> [hidden email]
>
> http://www.biology.missouri.edu/faculty/phillips.html
> http://microscopy.missouri.edu
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Kumar, Niyanta
> Sent: Thursday, October 17, 2019 2:12 PM
> To: [hidden email]
> Subject: Re: Confocal and Widefield microscopy training material
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and
> excellent training resources to go through! Much appreciated 😊
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Steffen Dietzel
> Sent: Thursday, October 17, 2019 6:55 AM
> To: [hidden email]
> Subject: Re: Confocal and Widefield microscopy training material
>
> EXTERNAL EMAIL – Use caution with any links or file attachments.
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Niyanta,
>
> I have collected a few resources on our website, so that I can refer our
> users to it more easily. This is at
> https://www.bioimaging.bmc.med.uni-muenchen.de/learn/
> Have a look at "Educational Websites" and "Materials for teaching".
> Maybe you find something helpful. The listed page from Australia (was
> mentioned by others too) contains a quiz that you can take, and you can
> print out a certificate if you answer 8+ of 10 questions correct.
>
> As in terms of hands on training, we start with a 3 h introduction session
> for up to 3 people. Usually with our samples, for two reasons.
> First, we want the user to pay attention to the microscope and software,
> not on the biological content of the images we make. Second, this way we
> can be sure that the sample is all right and we don't have users with
> crappy samples blaming it on the microscope. I agree with others that after
> 3 hours it makes no more sense, brains are full.
>
> Next we do a 'guided session' with the user's sample that we accompany
> until the user is getting good results. This is 1:1. Depending on sample
> difficulty, prior knowledge and user talent we sometimes have a third
> session.
>
> I am impressed by Sylvie's approach, but I doubt our group leaders would
> be willing to come up with so much money for training. Some think what we
> do now is already too time consuming (but most are happy about it).
>
>
> Steffen
>
> Am 11.10.2019 um 21:59 schrieb Niyanta Kumar:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi all,
> > Can someone suggest any existing resources and formats to pull from
> > for designing new and advanced user trainings for confocal and
> > widefield microscopy? I need to design trainings for a Zeiss LSM 980
> > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive
> > resources or existing quizzes that can ensure users know what they
> > need to would also be helpful.
> > Thanks,
> > Niyanta
> >
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Biomedical Center (BMC)
> Head of the Core Facility Bioimaging
>
> Großhaderner Straße 9
> D-82152 Planegg-Martinsried
> Germany
>
> http://www.bioimaging.bmc.med.uni-muenchen.de
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New
> Jersey, USA 07033), and/or its affiliates Direct contact information for
> affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error, please
> notify us immediately by reply e-mail and then delete it from your system.
>
>
Oshel, Philip Eugene Oshel, Philip Eugene
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Re: Confocal and Widefield microscopy training material

In reply to this post by Phillips, Thomas E.
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

We teach a grad-level microscopy course in confocal (also SEM & TEM courses). Tuition dollars don't come to the facility, but the instrument use (student use hours) is billed to the course, and that money does come to the facility. The instruments are billed at the standard "in-house" rate.
Note: This are the same courses the undergrads in our "microscopy major" take.

Phil
-------------
Philip Oshel    
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> on behalf of "Phillips, Thomas E." <[hidden email]>
Reply-To: Confocal Microscopy List <[hidden email]>
Date: Thursday,  17October, 2019 at 15:34
To: "[hidden email]" <[hidden email]>
Subject: Re: Confocal and Widefield microscopy training material

    *****
    To join, leave or search the confocal microscopy listserv, go to:
    http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    Post images on http://www.imgur.com and include the link in your posting.
    *****
   
    Does anybody offer their confocal training as an official grad-level class? In the past, offering a hands-on class was problematic for us since (i) no tuition dollars flow back to the core and (ii) PI's wanted their students using their own samples so "free class time" meant no income for us when those projects would normally have been done outside of class. Our campus is moving to a new budget model and tuition dollars may flow more directly back to us. We are considering combining training and supervised independent use as part of the class design and allow student efforts to be credentialed. I would be interested to know if anyone else is taking this approach and how many credit hours you offer and what you cover as part of the course.
   
    I have never met a PI who would have paid for Sylvie's design and impressed her clients do so.
   
   
   
    Thomas E. Phillips, Ph.D
    Professor of Biological Sciences
    Curators’ Distinguished Teaching Professor
    Director, Molecular Cytology Core
    2 Tucker Hall
    University of Missouri
    Columbia, MO 65211-7400
    573-882-4712 (office)
    573-882-0123 (fax)
    [hidden email]
   
    http://www.biology.missouri.edu/faculty/phillips.html
    http://microscopy.missouri.edu 
   
    -----Original Message-----
    From: Confocal Microscopy List <[hidden email]> On Behalf Of Kumar, Niyanta
    Sent: Thursday, October 17, 2019 2:12 PM
    To: [hidden email]
    Subject: Re: Confocal and Widefield microscopy training material
   
    *****
    To join, leave or search the confocal microscopy listserv, go to:
    http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    Post images on http://www.imgur.com and include the link in your posting.
    *****
   
    Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊
   
    -----Original Message-----
    From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel
    Sent: Thursday, October 17, 2019 6:55 AM
    To: [hidden email]
    Subject: Re: Confocal and Widefield microscopy training material
   
    EXTERNAL EMAIL – Use caution with any links or file attachments.
   
    *****
    To join, leave or search the confocal microscopy listserv, go to:
    http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    Post images on http://www.imgur.com and include the link in your posting.
    *****
   
    Hi Niyanta,
   
    I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://www.bioimaging.bmc.med.uni-muenchen.de/learn/
    Have a look at "Educational Websites" and "Materials for teaching".
    Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct.
   
    As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons.
    First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full.
   
    Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session.
   
    I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it).
   
   
    Steffen
   
    Am 11.10.2019 um 21:59 schrieb Niyanta Kumar:
    > *****
    > To join, leave or search the confocal microscopy listserv, go to:
    > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    > Post images on http://www.imgur.com and include the link in your posting.
    > *****
    >
    > Hi all,
    > Can someone suggest any existing resources and formats to pull from
    > for designing new and advanced user trainings for confocal and
    > widefield microscopy? I need to design trainings for a Zeiss LSM 980
    > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive
    > resources or existing quizzes that can ensure users know what they
    > need to would also be helpful.
    > Thanks,
    > Niyanta
    >
    --
    ------------------------------------------------------------
    Steffen Dietzel, PD Dr. rer. nat
    Ludwig-Maximilians-Universität München
    Biomedical Center (BMC)
    Head of the Core Facility Bioimaging
   
    Großhaderner Straße 9
    D-82152 Planegg-Martinsried
    Germany
   
    http://www.bioimaging.bmc.med.uni-muenchen.de
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Cammer, Michael Cammer, Michael
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Re: Confocal and Widefield microscopy training material

*****
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*****

I have pinch hit lectured and ran labs in many courses.  Sometimes the instrument time was paid for out of an education budget, but mostly not.  The core (or me personally since most lectures were prepared at home) absorbed the costs of my time.  

I also taught two years of a week-long course for new grad students.  I sent bills, but don’t know if they were actually paid.

Teaching does bring in business.  Students who find our teaching useful or interesting spread the word.

Cheers-
Michael Cammer

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Oshel, Philip Eugene
Sent: Thursday, October 17, 2019 3:47 PM
To: [hidden email]
Subject: Re: Confocal and Widefield microscopy training material

*****
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*****

We teach a grad-level microscopy course in confocal (also SEM & TEM courses). Tuition dollars don't come to the facility, but the instrument use (student use hours) is billed to the course, and that money does come to the facility. The instruments are billed at the standard "in-house" rate.
Note: This are the same courses the undergrads in our "microscopy major" take.

Phil
-------------
Philip Oshel    
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> on behalf of "Phillips, Thomas E." <[hidden email]>
Reply-To: Confocal Microscopy List <[hidden email]>
Date: Thursday,  17October, 2019 at 15:34
To: "[hidden email]" <[hidden email]>
Subject: Re: Confocal and Widefield microscopy training material

    *****
    To join, leave or search the confocal microscopy listserv, go to:
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    Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=Mal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE&e=  and include the link in your posting.
    *****
   
    Does anybody offer their confocal training as an official grad-level class? In the past, offering a hands-on class was problematic for us since (i) no tuition dollars flow back to the core and (ii) PI's wanted their students using their own samples so "free class time" meant no income for us when those projects would normally have been done outside of class. Our campus is moving to a new budget model and tuition dollars may flow more directly back to us. We are considering combining training and supervised independent use as part of the class design and allow student efforts to be credentialed. I would be interested to know if anyone else is taking this approach and how many credit hours you offer and what you cover as part of the course.
   
    I have never met a PI who would have paid for Sylvie's design and impressed her clients do so.
   
   
   
    Thomas E. Phillips, Ph.D
    Professor of Biological Sciences
    Curators’ Distinguished Teaching Professor
    Director, Molecular Cytology Core
    2 Tucker Hall
    University of Missouri
    Columbia, MO 65211-7400
    573-882-4712 (office)
    573-882-0123 (fax)
    [hidden email]
   
    https://urldefense.proofpoint.com/v2/url?u=http-3A__www.biology.missouri.edu_faculty_phillips.html&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=2X2JzDknmfdEUySiYs80Xwo3E8_H7XOtsRvsqjyk-as&e= 
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    -----Original Message-----
    From: Confocal Microscopy List <[hidden email]> On Behalf Of Kumar, Niyanta
    Sent: Thursday, October 17, 2019 2:12 PM
    To: [hidden email]
    Subject: Re: Confocal and Widefield microscopy training material
   
    *****
    To join, leave or search the confocal microscopy listserv, go to:
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    Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=Mal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE&e=  and include the link in your posting.
    *****
   
    Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊
   
    -----Original Message-----
    From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel
    Sent: Thursday, October 17, 2019 6:55 AM
    To: [hidden email]
    Subject: Re: Confocal and Widefield microscopy training material
   
    EXTERNAL EMAIL – Use caution with any links or file attachments.
   
    *****
    To join, leave or search the confocal microscopy listserv, go to:
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    *****
   
    Hi Niyanta,
   
    I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_learn_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=8ICIFmvnIJoBPS2mAi7Ci-kXZCRIY5Bv8knrcEHDEtQ&e= 
    Have a look at "Educational Websites" and "Materials for teaching".
    Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct.
   
    As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons.
    First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full.
   
    Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session.
   
    I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it).
   
   
    Steffen
   
    Am 11.10.2019 um 21:59 schrieb Niyanta Kumar:
    > *****
    > To join, leave or search the confocal microscopy listserv, go to:
    > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=AQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8&e= 
    > Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=Mal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE&e=  and include the link in your posting.
    > *****
    >
    > Hi all,
    > Can someone suggest any existing resources and formats to pull from
    > for designing new and advanced user trainings for confocal and
    > widefield microscopy? I need to design trainings for a Zeiss LSM 980
    > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive
    > resources or existing quizzes that can ensure users know what they
    > need to would also be helpful.
    > Thanks,
    > Niyanta
    >
    --
    ------------------------------------------------------------
    Steffen Dietzel, PD Dr. rer. nat
    Ludwig-Maximilians-Universität München
    Biomedical Center (BMC)
    Head of the Core Facility Bioimaging
   
    Großhaderner Straße 9
    D-82152 Planegg-Martinsried
    Germany
   
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