Confocal and Widefield microscopy training material
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Niyanta Kumar |
Confocal and Widefield microscopy training material
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi all, Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful. Thanks, Niyanta |
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Sylvie Le Guyader |
Re: Confocal and Widefield microscopy training material
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Niyanta Alyona Minina has tons of videos on you tube. Check her channel<https://www.youtube.com/channel/UCfRx0TPF-R1TKYrPPu7OwWA/videos>. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Niyanta Kumar Sent: 11 October 2019 22:00 To: [hidden email] Subject: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C87a9cd3e2b2d42366e3808d74e8707d5%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637064214752285084&sdata=XtByjWY8X%2BLYVFhPt7T7XqnizH6v9I0RZalnJIYo23s%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C87a9cd3e2b2d42366e3808d74e8707d5%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637064214752285084&sdata=6m3Wpvhetv%2BypOIa61Jr8jZeKn0olhUe50y8LbuvJho%3D&reserved=0 and include the link in your posting. ***** Hi all, Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful. Thanks, Niyanta När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>. |
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Arvydas Matiukas |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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Sylvie Le Guyader |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** ’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’ Definitely! That would bore me too and I am not from the millennium generation! 😉 I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. 😊 Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility> Follow our microscopy blog!<http://microscopykarolinska.se/> From: Arvydas Matiukas <[hidden email]> Sent: 16 October 2019 13:53 To: Sylvie Le Guyader <[hidden email]>; [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material Hello Sylvie, Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3. with best wishes Arvydas +++++++++++++++++++++++++++++ Arvydas Matiukas, Ph.D. Manager of NRB Shared Equipment Director of Confocal&Two-Photon Core SUNY Upstate Medical University >>> Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=eFtCsX4RwhV6lbXscD96MNTyssit68QdB4fZA95hrQQ&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=fAjOkG_slD2KBsdz68xI4iL4ibgq5pezOL5F3lRr2y4&e= and include the link in your posting. ***** Hi Niyanta Alyona Minina has tons of videos on you tube. Check her channel<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e=%20> >. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Niyanta Kumar Sent: 11 October 2019 22:00 To: [hidden email]<mailto:[hidden email]> Subject: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Flists.umn.edu-252Fcgi-2Dbin-252Fwa-253FA0-253Dconfocalmicroscopy-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C87a9cd3e2b2d42366e3808d74e8707d5-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637064214752285084-26amp-3Bsdata-3DXtByjWY8X-252BLYVFhPt7T7XqnizH6v9I0RZalnJIYo23s-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=9ndwMylZEtR6J8pLSr5vVXCqk8bBKkmliAmW2jootR0&e= Post images on https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Fwww.imgur.com-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C87a9cd3e2b2d42366e3808d74e8707d5-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637064214752285084-26amp-3Bsdata-3D6m3Wpvhetv-252BypOIa61Jr8jZeKn0olhUe50y8LbuvJho-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=pZwg3cOxtPEB45-6ZJuKjpBvaR4hMvAION4xiM1-TXU&e= and include the link in your posting. ***** Hi all, Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful. Thanks, Niyanta När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Hr finns information om hur KI behandlar personuppgifter<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=EQs1kTVGSWHn1Bbnwf8tsm9ZmeiX9rnpVF3Gx8njx2g&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=EQs1kTVGSWHn1Bbnwf8tsm9ZmeiX9rnpVF3Gx8njx2g&e=%20> >. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=3Bvbvriv0Qsm1rvTGdCTn69BFi2whwW0uFp_0iJEm9g&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=3Bvbvriv0Qsm1rvTGdCTn69BFi2whwW0uFp_0iJEm9g&e=%20> >. När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>. |
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Niyanta Kumar |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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In reply to this post by Niyanta Kumar
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thanks Sylvie, this is helpful! Niyanta Kumar, Ph.D. Senior Scientist - Pharmacokinetics and ADME Merck Research Laboratories -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Sylvie Le Guyader Sent: Wednesday, October 16, 2019 9:55 AM To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material EXTERNAL EMAIL – Use caution with any links or file attachments. ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** ’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’ Definitely! That would bore me too and I am not from the millennium generation! 😉 I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. 😊 Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility> Follow our microscopy blog!<http://microscopykarolinska.se/> From: Arvydas Matiukas <[hidden email]> Sent: 16 October 2019 13:53 To: Sylvie Le Guyader <[hidden email]>; [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material Hello Sylvie, Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3. with best wishes Arvydas +++++++++++++++++++++++++++++ Arvydas Matiukas, Ph.D. Manager of NRB Shared Equipment Director of Confocal&Two-Photon Core SUNY Upstate Medical University >>> Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=eFtCsX4RwhV6lbXscD96MNTyssit68QdB4fZA95hrQQ&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=fAjOkG_slD2KBsdz68xI4iL4ibgq5pezOL5F3lRr2y4&e= and include the link in your posting. ***** Hi Niyanta Alyona Minina has tons of videos on you tube. Check her channel<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e=%20> >. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Niyanta Kumar Sent: 11 October 2019 22:00 To: [hidden email]<mailto:[hidden email]> Subject: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Flists.umn.edu-252Fcgi-2Dbin-252Fwa-253FA0-253Dconfocalmicroscopy-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C87a9cd3e2b2d42366e3808d74e8707d5-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637064214752285084-26amp-3Bsdata-3DXtByjWY8X-252BLYVFhPt7T7XqnizH6v9I0RZalnJIYo23s-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=9ndwMylZEtR6J8pLSr5vVXCqk8bBKkmliAmW2jootR0&e= Post images on https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Fwww.imgur.com-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C87a9cd3e2b2d42366e3808d74e8707d5-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637064214752285084-26amp-3Bsdata-3D6m3Wpvhetv-252BypOIa61Jr8jZeKn0olhUe50y8LbuvJho-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=pZwg3cOxtPEB45-6ZJuKjpBvaR4hMvAION4xiM1-TXU&e= and include the link in your posting. ***** Hi all, Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful. Thanks, Niyanta När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Hr finns information om hur KI behandlar personuppgifter<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=EQs1kTVGSWHn1Bbnwf8tsm9ZmeiX9rnpVF3Gx8njx2g&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=EQs1kTVGSWHn1Bbnwf8tsm9ZmeiX9rnpVF3Gx8njx2g&e=%20> >. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=3Bvbvriv0Qsm1rvTGdCTn69BFi2whwW0uFp_0iJEm9g&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=3Bvbvriv0Qsm1rvTGdCTn69BFi2whwW0uFp_0iJEm9g&e=%20> >. När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>. Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. |
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Arvydas Matiukas |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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Sylvie Le Guyader |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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Cammer, Michael |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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In reply to this post by Arvydas Matiukas
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** The discussions on microscopy training are extremely valuable. I need to review all the replies again and, based on them, revise the rules I posted, modify how we do training, and write directions for new users to read before training. In response to Arvydas's question about trainees, I find that status as student, postdoc, technician, or PI has no bearing on existing expertise or willingness or ability to learn the material. Why or how people learn diverge widely based on many factors. Small groups of trainees is good for saving time on the material that can be shown and discussed, however, only the person who gets to drive the machine may be ready to use it unassisted. Everyone else needs to follow up with practical training. Cheers- Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016 Office: 646-501-0567 Cell: 914-309-3270 [hidden email] http://nyulmc.org/micros http://microscopynotes.com/ -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Arvydas Matiukas Sent: Wednesday, October 16, 2019 11:46 AM To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material Hi Silvie, That's useful approach to try. Whenever possible I try an individual approach matching the scope and depth of the material to the trainee's background and skill level. The worst scenario is when a lab sends in several trainees of different level (e.g. student, technician and postdoc). Do you accept several trainees for the same session? Next major difficulty for the trainee that I noticed is the transition from imaging training sample to the self-prepared one. Here the ability to duplicate tutorial actions usually is not enough , in depth understanding is required, and of course preparing quality samples. Best regards, Arvydas >>> Sylvie Le Guyader <[hidden email]> 10/16/2019 9:54 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=XGh1Uu87TWxekpsyPOgE9NKlXFtrItrNJpVgKlIIhEk&s=1I4RBPK585IqkSCgWSgQlrCupPknMSbFrCOaHZSqDEk&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=XGh1Uu87TWxekpsyPOgE9NKlXFtrItrNJpVgKlIIhEk&s=Xzk8Ro7YdVBAzZ37NqwqGMI3eVf5cVL34lfiz_pL-ZY&e= and include the link in your posting. ***** ’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’ Definitely! That would bore me too and I am not from the millennium generation! I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_bionut_welcome-2Dto-2Dthe-2Dlci-2Dfacility&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=XGh1Uu87TWxekpsyPOgE9NKlXFtrItrNJpVgKlIIhEk&s=eHHGZSUe6-p2KrVzjC_VHcLg3gh9nGLmDr6tud4_KTE&e= > Follow our microscopy blog!<https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopykarolinska.se_&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=XGh1Uu87TWxekpsyPOgE9NKlXFtrItrNJpVgKlIIhEk&s=4wwhhmKRfu8gO9SaXZJKMAf72Q8Ng7Jhnh3SLgiIFn4&e= > From: Arvydas Matiukas <[hidden email]> Sent: 16 October 2019 13:53 To: Sylvie Le Guyader <[hidden email]>; [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material Hello Sylvie, Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3. with best wishes Arvydas +++++++++++++++++++++++++++++ Arvydas Matiukas, Ph.D. Manager of NRB Shared Equipment Director of Confocal&Two-Photon Core SUNY Upstate Medical University >>> Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=eFtCsX4RwhV6lbXscD96MNTyssit68QdB4fZA95hrQQ&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=fAjOkG_slD2KBsdz68xI4iL4ibgq5pezOL5F3lRr2y4&e= and include the link in your posting. ***** Hi Niyanta Alyona Minina has tons of videos on you tube. Check her channel<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.youtube.com_channel_UCfRx0TPF-2DR1TKYrPPu7OwWA_videos&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=o-JPkTgkyERoEbgIwRA5ZY6W1TvsWK5gsmDv1ge4toE&e=%20> >. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Niyanta Kumar Sent: 11 October 2019 22:00 To: [hidden email]<mailto:[hidden email]> Subject: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Flists.umn.edu-252Fcgi-2Dbin-252Fwa-253FA0-253Dconfocalmicroscopy-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C87a9cd3e2b2d42366e3808d74e8707d5-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637064214752285084-26amp-3Bsdata-3DXtByjWY8X-252BLYVFhPt7T7XqnizH6v9I0RZalnJIYo23s-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=9ndwMylZEtR6J8pLSr5vVXCqk8bBKkmliAmW2jootR0&e= Post images on https://urldefense.proofpoint.com/v2/url?u=https-3A__eur01.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Fwww.imgur.com-26amp-3Bdata-3D02-257C01-257Csylvie.le.guyader-2540KI.SE-257C87a9cd3e2b2d42366e3808d74e8707d5-257Cbff7eef1cf4b4f32be3da1dda043c05d-257C0-257C0-257C637064214752285084-26amp-3Bsdata-3D6m3Wpvhetv-252BypOIa61Jr8jZeKn0olhUe50y8LbuvJho-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=pZwg3cOxtPEB45-6ZJuKjpBvaR4hMvAION4xiM1-TXU&e= and include the link in your posting. ***** Hi all, Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful. Thanks, Niyanta När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Hr finns information om hur KI behandlar personuppgifter<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=EQs1kTVGSWHn1Bbnwf8tsm9ZmeiX9rnpVF3Gx8njx2g&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=EQs1kTVGSWHn1Bbnwf8tsm9ZmeiX9rnpVF3Gx8njx2g&e=%20> >. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=3Bvbvriv0Qsm1rvTGdCTn69BFi2whwW0uFp_0iJEm9g&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=fqPKyH7OvDC2YG-CgHu2VMvZCDwOwF5ALyEnXO-YnB8&s=3Bvbvriv0Qsm1rvTGdCTn69BFi2whwW0uFp_0iJEm9g&e=%20> >. När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_medarbetare_integritetsskyddspolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=XGh1Uu87TWxekpsyPOgE9NKlXFtrItrNJpVgKlIIhEk&s=8j7d7fhNJQoj3Rai2nIAydL3Me-74TRwHI-43to-b0M&e= >. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy&d=DwIGaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=XGh1Uu87TWxekpsyPOgE9NKlXFtrItrNJpVgKlIIhEk&s=qzKolo6QFEOyQO9RlKOPvFp7_-GjfPYp-nmKl8nobas&e= >. |
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Arvydas Matiukas |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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Jacqueline Ross |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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Neftali Flores Rodriguez |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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In reply to this post by Niyanta Kumar
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Niyanta, You might find our MyScope useful. https://myscope.training/ Neftali Flores-Rodriguez | Light Microscopist Sydney Microscopy and Microanalysis (SMM) Australian Centre for Microscopy & Microanalysis (ACMM) THE UNIVERSITY OF SYDNEY ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Kumar, Niyanta <[hidden email]> Sent: Wednesday, 16 October 2019, 6:04 pm To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu Post images on https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com and include the link in your posting. ***** Thanks Sylvie, this is helpful! Niyanta Kumar, Ph.D. Senior Scientist - Pharmacokinetics and ADME Merck Research Laboratories -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Sylvie Le Guyader Sent: Wednesday, October 16, 2019 9:55 AM To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material EXTERNAL EMAIL – Use caution with any links or file attachments. ***** To join, leave or search the confocal microscopy listserv, go to: https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu Post images on https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com and include the link in your posting. ***** ’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’ Definitely! That would bore me too and I am not from the millennium generation! 😉 I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. 😊 Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website<https://protect-au.mimecast.com/s/40xyCMwvLQTAQG37hJE2HF?domain=ki.se> Follow our microscopy blog!<https://protect-au.mimecast.com/s/vt_4CNLwM9iG213zIRmKQP?domain=microscopykarolinska.se> From: Arvydas Matiukas <[hidden email]> Sent: 16 October 2019 13:53 To: Sylvie Le Guyader <[hidden email]>; [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material Hello Sylvie, Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3. with best wishes Arvydas +++++++++++++++++++++++++++++ Arvydas Matiukas, Ph.D. Manager of NRB Shared Equipment Director of Confocal&Two-Photon Core SUNY Upstate Medical University >>> Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://protect-au.mimecast.com/s/k0SUCOMxNyty6oxMcP_F05?domain=urldefense.proofpoint.com Post images on https://protect-au.mimecast.com/s/F4k8CP7yOZtk7qXPIrKiv7?domain=urldefense.proofpoint.com and include the link in your posting. ***** Hi Niyanta Alyona Minina has tons of videos on you tube. Check her channel<https://protect-au.mimecast.com/s/Dn7vCQnzP0ty5mPYc99xjB?domain=urldefense.proofpoint.com <https://protect-au.mimecast.com/s/dOszCROAQotzA8WxT05sni?domain=urldefense.proofpoint.com> >. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Niyanta Kumar Sent: 11 October 2019 22:00 To: [hidden email]<mailto:[hidden email]> Subject: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://protect-au.mimecast.com/s/UPl4CVAGXPtRD1yYIr34h8?domain=urldefense.proofpoint.com Post images on https://protect-au.mimecast.com/s/gH1FCWLJY7iWAvqVFBZlT4?domain=urldefense.proofpoint.com and include the link in your posting. ***** Hi all, Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful. Thanks, Niyanta När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Hr finns information om hur KI behandlar personuppgifter<https://protect-au.mimecast.com/s/08zqCXLKZoizx3KWTx7TnX?domain=urldefense.proofpoint.com <https://protect-au.mimecast.com/s/XRVlCYWL1vi4zPlwurPdw1?domain=urldefense.proofpoint.com> >. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://protect-au.mimecast.com/s/2FL4CZYM2VFp3yg0HVbRoD?domain=urldefense.proofpoint.com <https://protect-au.mimecast.com/s/WnEEC1WZXri97KGlIKjaFh?domain=urldefense.proofpoint.com> >. När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://protect-au.mimecast.com/s/2ZpGC2xZYvC9NA3gIWN0gM?domain=ki.se>. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://protect-au.mimecast.com/s/FH5gC3Q8Z2FE5VKOsP5zcG?domain=ki.se>. Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at https://protect-au.mimecast.com/s/msH2C4QZ1RFvpG5qsQAwax?domain=merck.com that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. |
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Katherina Garcia Garcia |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hello, https://www.ibiology.org It is wonderful. It has a microscopy course with more than 70 talks given by experts in the field, including Nobel Prize winners. Enjoy it! Kathy El 2019-10-17 06:13, Neftali Flores Rodriguez escribió: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Niyanta, > > You might find our MyScope useful. > > https://myscope.training/ > > Neftali Flores-Rodriguez | Light Microscopist > > Sydney Microscopy and Microanalysis (SMM) > Australian Centre for Microscopy & Microanalysis (ACMM) > > THE UNIVERSITY OF SYDNEY > > ________________________________ > From: Confocal Microscopy List <[hidden email]> on behalf of Kumar, Niyanta <[hidden email]> > Sent: Wednesday, 16 October 2019, 6:04 pm > To: [hidden email] > Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material > > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu > Post images on https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com and include the link in your posting. > ***** > > Thanks Sylvie, this is helpful! > > Niyanta Kumar, Ph.D. > Senior Scientist - Pharmacokinetics and ADME > Merck Research Laboratories > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> On Behalf Of Sylvie Le Guyader > Sent: Wednesday, October 16, 2019 9:55 AM > To: [hidden email] > Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material > > EXTERNAL EMAIL - Use caution with any links or file attachments. > > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://protect-au.mimecast.com/s/gitfCK1qJZtGj0W6IM_kIr?domain=lists.umn.edu > Post images on https://protect-au.mimecast.com/s/cEnICL7rK8tZJ597SqQgXt?domain=imgur.com and include the link in your posting. > ***** > > ' significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.' > Definitely! That would bore me too and I am not from the millennium generation! 😉 > I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it. 😊 > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader, PhD > Live Cell Imaging Facility Manager > Karolinska Institutet- Bionut Dpt > Blickagången 16, > Room 7362 (lab)/7840 (office) > 14157 Huddinge, Sweden > mobile: +46 (0) 73 733 5008 > LCI website<https://protect-au.mimecast.com/s/40xyCMwvLQTAQG37hJE2HF?domain=ki.se> > Follow our microscopy blog!<https://protect-au.mimecast.com/s/vt_4CNLwM9iG213zIRmKQP?domain=microscopykarolinska.se> > > From: Arvydas Matiukas <[hidden email]> > Sent: 16 October 2019 13:53 > To: Sylvie Le Guyader <[hidden email]>; [hidden email] > Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material > > Hello Sylvie, > > Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3. > > with best wishes Arvydas > > +++++++++++++++++++++++++++++ > Arvydas Matiukas, Ph.D. > Manager of NRB Shared Equipment > Director of Confocal&Two-Photon Core > SUNY Upstate Medical University > > Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> 10/16/19 6:28 AM >>> To join, leave or search the confocal microscopy listserv, go to: https://protect-au.mimecast.com/s/k0SUCOMxNyty6oxMcP_F05?domain=urldefense.proofpoint.com Post images on https://protect-au.mimecast.com/s/F4k8CP7yOZtk7qXPIrKiv7?domain=urldefense.proofpoint.com and include the link in your posting. ***** Hi Niyanta Alyona Minina has tons of videos on you tube. Check her channel<https://protect-au.mimecast.com/s/Dn7vCQnzP0ty5mPYc99xjB?domain=urldefense.proofpoint.com <https://protect-au.mimecast.com/s/dOszCROAQotzA8WxT05sni?domain=urldefense.proofpoint.com> >. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Niyanta Kumar Sent: 11 October 2019 22:00 To: [hidden email]<mailto:[hidden email]> Subject: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://protect-au.mimecast.com/s/UPl4CVAGXPtRD1yYIr34h8?domain=urldefense.proofpoint.com Post images on https://protect-au.mimecast.com/s/gH1FCWLJY7iWAvqVFBZlT4?domain=urldefense.proofpoint.com and include the link in your posting. ***** Hi all, Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful. Thanks, Niyanta När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Hr finns information om hur KI behandlar personuppgifter<https://protect-au.mimecast.com/s/08zqCXLKZoizx3KWTx7TnX?domain=urldefense.proofpoint.com <https://protect-au.mimecast.com/s/XRVlCYWL1vi4zPlwurPdw1?domain=urldefense.proofpoint.com> >. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI's processing of personal data here<https://protect-au.mimecast.com/s/2FL4CZYM2VFp3yg0HVbRoD?domain=urldefense.proofpoint.com <https://protect-au.mimecast.com/s/WnEEC1WZXri97KGlIKjaFh?domain=urldefense.proofpoint.com> >. När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://protect-au.mimecast.com/s/2ZpGC2xZYvC9NA3gIWN0gM?domain=ki.se>. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI's processing of personal data here<https://protect-au.mimecast.com/s/FH5gC3Q8Z2FE5VKOsP5zcG?domain=ki.se>. Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at https://protect-au.mimecast.com/s/msH2C4QZ1RFvpG5qsQAwax?domain=merck.com that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. 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Steffen Dietzel |
Re: Confocal and Widefield microscopy training material
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In reply to this post by Niyanta Kumar
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://www.bioimaging.bmc.med.uni-muenchen.de/learn/ Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany http://www.bioimaging.bmc.med.uni-muenchen.de |
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Sylvie Le Guyader |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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In reply to this post by Katherina Garcia Garcia
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Jennifer Waters microscopy microcourses on Youtube. Short and excellent. https://www.youtube.com/channel/UC4cOKa0TZK8CQhzSQqfwuMQ Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Katherina Garcia Garcia Sent: 17 October 2019 09:46 To: [hidden email] Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131008813&sdata=KhY0Yn%2FI5VdMkbVoQrTmP%2F%2ByPfSvo5DRtMY2M0giUVQ%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=jmFqcCAKs5yeew1MK%2BT7ewOzO1vx1Nhd8wp4B1qnr1Y%3D&reserved=0 and include the link in your posting. ***** Hello, https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ibiology.org&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=Rwt5ADH9k1ytvIUh3PA4VOPFowJ6a8HWz%2BZhwVR4IOU%3D&reserved=0 It is wonderful. It has a microscopy course with more than 70 talks given by experts in the field, including Nobel Prize winners. Enjoy it! Kathy El 2019-10-17 06:13, Neftali Flores Rodriguez escribió: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists > .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Cs > ylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1 > cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=QmWnsV > bFLuoNJ9V25urRLIHen%2B%2FQTGluRAxQ6g7gwnA%3D&reserved=0 > Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=jmFqcCAKs5yeew1MK%2BT7ewOzO1vx1Nhd8wp4B1qnr1Y%3D&reserved=0 and include the link in your posting. > ***** > > Hi Niyanta, > > You might find our MyScope useful. > > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fmysc > ope.training%2F&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd5 > 9bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7 > C637068958131018805&sdata=iEot7zPhgZ0n2mSKIWcWVZ%2FABpnncOl61ZSetK > lUQg0%3D&reserved=0 > > Neftali Flores-Rodriguez | Light Microscopist > > Sydney Microscopy and Microanalysis (SMM) Australian Centre for > Microscopy & Microanalysis (ACMM) > > THE UNIVERSITY OF SYDNEY > > ________________________________ > From: Confocal Microscopy List <[hidden email]> on > behalf of Kumar, Niyanta > <[hidden email]> > Sent: Wednesday, 16 October 2019, 6:04 pm > To: [hidden email] > Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training > material > > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprot > ect-au.mimecast.com%2Fs%2FgitfCK1qJZtGj0W6IM_kIr%3Fdomain%3Dlists.umn. > edu&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865 > 508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C63706895813 > 1018805&sdata=QzqA8Yq7Qc1Hwhw1X5dnnnJUZt5qMLFWTH428zWyIUM%3D&r > eserved=0 Post images on > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprotect-au.mimecast.com%2Fs%2FcEnICL7rK8tZJ597SqQgXt%3Fdomain%3Dimgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=z5NMvmYN9TUA62JZ%2B4sqnDu9ZOjVyDDLRyNJC6Y2ZSA%3D&reserved=0 and include the link in your posting. > ***** > > Thanks Sylvie, this is helpful! > > Niyanta Kumar, Ph.D. > Senior Scientist - Pharmacokinetics and ADME Merck Research > Laboratories > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> On > Behalf Of Sylvie Le Guyader > Sent: Wednesday, October 16, 2019 9:55 AM > To: [hidden email] > Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training > material > > EXTERNAL EMAIL - Use caution with any links or file attachments. > > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprot > ect-au.mimecast.com%2Fs%2FgitfCK1qJZtGj0W6IM_kIr%3Fdomain%3Dlists.umn. > edu&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865 > 508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C63706895813 > 1018805&sdata=QzqA8Yq7Qc1Hwhw1X5dnnnJUZt5qMLFWTH428zWyIUM%3D&r > eserved=0 Post images on > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprotect-au.mimecast.com%2Fs%2FcEnICL7rK8tZJ597SqQgXt%3Fdomain%3Dimgur.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=z5NMvmYN9TUA62JZ%2B4sqnDu9ZOjVyDDLRyNJC6Y2ZSA%3D&reserved=0 and include the link in your posting. > ***** > > ' significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.' > Definitely! That would bore me too and I am not from the millennium > generation! 😉 > I believe that the only way is to make very short videos and give > quizzes half way or just afterwards. E.g. our trainee watches our > video about bit depth, saturation and under exposure knowing that > right after the video we will ask to show us where the buttons are and > if there was any saturation/under exposure in the image they took > immediately before watching the video. They are encouraged to stop the > video and push the buttons on the software whenever they feel like it. > 😊 > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader, PhD > Live Cell Imaging Facility Manager > Karolinska Institutet- Bionut Dpt > Blickagången 16, > Room 7362 (lab)/7840 (office) > 14157 Huddinge, Sweden > mobile: +46 (0) 73 733 5008 > LCI > website<<a href="https://eur01.safelinks.protection.outlook.com/?url=https%3A%2">https://eur01.safelinks.protection.outlook.com/?url=https%3A%2 > F%2Fprotect-au.mimecast.com%2Fs%2F40xyCMwvLQTAQG37hJE2HF%3Fdomain%3Dki > .se&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865 > 508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C63706895813 > 1018805&sdata=SiFrrYL3Ao772BTc%2BoI7CfzSVsqO52FoLJPLGWzXj8A%3D& > ;reserved=0> Follow our microscopy > blog!<<a href="https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%">https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F% > 2Fprotect-au.mimecast.com%2Fs%2Fvt_4CNLwM9iG213zIRmKQP%3Fdomain%3Dmicr > oscopykarolinska.se&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163 > dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7 > C0%7C637068958131018805&sdata=1GO2O7ggkvx7%2ByKWc%2BlB6t64OTZjupbc > MMcLToMGCUA%3D&reserved=0> > > From: Arvydas Matiukas <[hidden email]> > Sent: 16 October 2019 13:53 > To: Sylvie Le Guyader <[hidden email]>; > [hidden email] > Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training > material > > Hello Sylvie, > > Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3. > > with best wishes Arvydas > > +++++++++++++++++++++++++++++ > Arvydas Matiukas, Ph.D. > Manager of NRB Shared Equipment > Director of Confocal&Two-Photon Core > SUNY Upstate Medical University > > Sylvie Le Guyader > <[hidden email]<mailto:[hidden email]>> 10/16/19 > 6:28 AM >>> To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprotect-au.mimecast.com%2Fs%2Fk0SUCOMxNyty6oxMcP_F05%3Fdomain%3Durldefense.proofpoint.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=Ratqq8b2%2F7%2F%2BcYm8%2B%2FSW8gbkdDpY2XmmrH4ibhi3pEM%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprotect-au.mimecast.com%2Fs%2FF4k8CP7yOZtk7qXPIrKiv7%3Fdomain%3Durldefense.proofpoint.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=8WETQcKUVKHui6AazG7o5qrjkt9zaur2OMLvLuGkZZ8%3D&reserved=0 and include the link in your posting. ***** Hi Niyanta Alyona Minina has tons of videos on you tube. Check her channel<https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprotect-au.mimecast.com%2Fs%2FDn7vCQnzP0ty5mPYc99xjB%3Fdomain%3Durldefense.proofpoint.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=4b0w7we4ofQ9aOSshTcsztwXWH4UXGice2Z%2BqQL%2FAMs%3D&reserved=0 <https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprotect-au.mimecast.com%2Fs%2FdOszCROAQotzA8WxT05sni%3Fdomain%3Durldefense.proofpoint.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=8Wsiqx5iE6GwjhbP2NT6NlJj3LiOJRb3XfAldb35kXw%3D&reserved=0> >. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Niyanta Kumar Sent: 11 October 2019 22:00 To: [hidden email]<mailto:[hidden email]> Subject: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprotect-au.mimecast.com%2Fs%2FUPl4CVAGXPtRD1yYIr34h8%3Fdomain%3Durldefense.proofpoint.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131018805&sdata=Oq8meJMZE6rILilVy8tCQhduJtP9AS6odpkbxcU5934%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fprotect-au.mimecast.com%2Fs%2FgH1FCWLJY7iWAvqVFBZlT4%3Fdomain%3Durldefense.proofpoint.com&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C163dfd59bcc44c33865508d752d79191%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068958131028802&sdata=ub5Fq%2FTj7iMGDenwMynDqH2z2Qvqg3GTFK5VdU%2FkVJg%3D&reserved=0 and include the link in your posting. ***** Hi all, Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful. Thanks, Niyanta När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. 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Arvydas Matiukas |
Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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In reply to this post by Steffen Dietzel
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Steffen, Silvie Thanks for very interesting and useful discussion. Few summary thoughts: 1. I second Steffens' reasoning for starting training with Core provided=known=calibrated samples. Sylvie's approach of starting with users'=non calibrated sample introduces to many unknowns at a time and to my opinion reduces efficiency of the training. I personally prefer not to be trained in such stressful way. 2. I wonder if many labs in EU can afford to spend 1 month of their member time and pay 1200EUR to get the members trained in advanced level microscopy. In the USA it definitely not going to happen on any significant scale. Maybe a cultural difference :) I recall the comparison provided by US based Zeiss specialist after his training in Germany: "problem= ballpoint pen stopped writing. German style solution = research potential causes, i.e. disassemble the pen, check if any ink is left, if ball rotates freely, finally if the user properly presses knob or rotates the body. This approach may take significant but reasonable time and provide useful skills and experience for the future. American style solution = check the pen price first. As it is much lower than the fee of professional spending hours on this problem then just trash the pen and get a new one" 3. Of course in the USA there are labs that need advanced level microscopy. However, typically they have their own microscopes and microscopy experts and very rarely use Core services. Among my 400 trainees over 15 years only about 10 expressed willingness to proceed to the advanced level, and among them only 1 grad student. 4. Finally, time and cost considerations. At 1 month and 1200EUR spending level how many techniques a beginner researcher may learn in 1 year ? 3years? BTW I think training in optical/confocal microscopy is relatively inexpensive, preparing your own samples typically is more time consuming and expensive, and learning other tecniques may be more expensive as well. My 2 cents, Arvydas +++++++++++++++++++++++++++++ Arvydas Matiukas, Ph.D. Manager of NRB Shared Equipment Director of Confocal&Two-Photon Core SUNY Upstate Medical University Neuroscience & Physiology Dept >>> Steffen Dietzel <[hidden email]> 10/17/2019 6:55 AM >>> ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=k0lBHjfBdP6gkIAbpZx0fMuOqb1oDSvuwF7Hk7nelgY&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=yAGFeShn7KSa-eW2mPu6XbhS9SeX7BD0-L0UF5cyjgE&e= and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_learn_&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=Z2jynrNg35the3m1H5X3AsUA52lnDqOb4qZUaoY05dY&e= Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=k0lBHjfBdP6gkIAbpZx0fMuOqb1oDSvuwF7Hk7nelgY&e= > Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=yAGFeShn7KSa-eW2mPu6XbhS9SeX7BD0-L0UF5cyjgE&e= and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität Mnchen Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany https://urldefense.proofpoint.com/v2/url?u=http-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=Gadulq06lK7kYY94plB3DsipjiGMo01xTiPc_poN3_Y&e= |
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Niyanta Kumar |
Re: Confocal and Widefield microscopy training material
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In reply to this post by Niyanta Kumar
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel Sent: Thursday, October 17, 2019 6:55 AM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material EXTERNAL EMAIL – Use caution with any links or file attachments. ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://www.bioimaging.bmc.med.uni-muenchen.de/learn/ Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany http://www.bioimaging.bmc.med.uni-muenchen.de Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. |
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Phillips, Thomas E. |
Re: Confocal and Widefield microscopy training material
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Does anybody offer their confocal training as an official grad-level class? In the past, offering a hands-on class was problematic for us since (i) no tuition dollars flow back to the core and (ii) PI's wanted their students using their own samples so "free class time" meant no income for us when those projects would normally have been done outside of class. Our campus is moving to a new budget model and tuition dollars may flow more directly back to us. We are considering combining training and supervised independent use as part of the class design and allow student efforts to be credentialed. I would be interested to know if anyone else is taking this approach and how many credit hours you offer and what you cover as part of the course. I have never met a PI who would have paid for Sylvie's design and impressed her clients do so. Thomas E. Phillips, Ph.D Professor of Biological Sciences Curators’ Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email] http://www.biology.missouri.edu/faculty/phillips.html http://microscopy.missouri.edu -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Kumar, Niyanta Sent: Thursday, October 17, 2019 2:12 PM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel Sent: Thursday, October 17, 2019 6:55 AM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material EXTERNAL EMAIL – Use caution with any links or file attachments. ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://www.bioimaging.bmc.med.uni-muenchen.de/learn/ Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany http://www.bioimaging.bmc.med.uni-muenchen.de Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. |
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Craig Brideau |
Re: Confocal and Widefield microscopy training material
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Here at University of Calgary we offer a Basic and an Advanced microscopy course, each taking a full semester. The Advanced class covers confocal and related techniques. We have had very good outcomes with graduate students who have completed these courses. Craig On Thu, Oct 17, 2019 at 1:34 PM Phillips, Thomas E. <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Does anybody offer their confocal training as an official grad-level > class? In the past, offering a hands-on class was problematic for us since > (i) no tuition dollars flow back to the core and (ii) PI's wanted their > students using their own samples so "free class time" meant no income for > us when those projects would normally have been done outside of class. Our > campus is moving to a new budget model and tuition dollars may flow more > directly back to us. We are considering combining training and supervised > independent use as part of the class design and allow student efforts to be > credentialed. I would be interested to know if anyone else is taking this > approach and how many credit hours you offer and what you cover as part of > the course. > > I have never met a PI who would have paid for Sylvie's design and > impressed her clients do so. > > > > Thomas E. Phillips, Ph.D > Professor of Biological Sciences > Curators’ Distinguished Teaching Professor > Director, Molecular Cytology Core > 2 Tucker Hall > University of Missouri > Columbia, MO 65211-7400 > 573-882-4712 (office) > 573-882-0123 (fax) > [hidden email] > > http://www.biology.missouri.edu/faculty/phillips.html > http://microscopy.missouri.edu > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> On > Behalf Of Kumar, Niyanta > Sent: Thursday, October 17, 2019 2:12 PM > To: [hidden email] > Subject: Re: Confocal and Widefield microscopy training material > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and > excellent training resources to go through! Much appreciated 😊 > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]> On > Behalf Of Steffen Dietzel > Sent: Thursday, October 17, 2019 6:55 AM > To: [hidden email] > Subject: Re: Confocal and Widefield microscopy training material > > EXTERNAL EMAIL – Use caution with any links or file attachments. > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Niyanta, > > I have collected a few resources on our website, so that I can refer our > users to it more easily. This is at > https://www.bioimaging.bmc.med.uni-muenchen.de/learn/ > Have a look at "Educational Websites" and "Materials for teaching". > Maybe you find something helpful. The listed page from Australia (was > mentioned by others too) contains a quiz that you can take, and you can > print out a certificate if you answer 8+ of 10 questions correct. > > As in terms of hands on training, we start with a 3 h introduction session > for up to 3 people. Usually with our samples, for two reasons. > First, we want the user to pay attention to the microscope and software, > not on the biological content of the images we make. Second, this way we > can be sure that the sample is all right and we don't have users with > crappy samples blaming it on the microscope. I agree with others that after > 3 hours it makes no more sense, brains are full. > > Next we do a 'guided session' with the user's sample that we accompany > until the user is getting good results. This is 1:1. Depending on sample > difficulty, prior knowledge and user talent we sometimes have a third > session. > > I am impressed by Sylvie's approach, but I doubt our group leaders would > be willing to come up with so much money for training. Some think what we > do now is already too time consuming (but most are happy about it). > > > Steffen > > Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hi all, > > Can someone suggest any existing resources and formats to pull from > > for designing new and advanced user trainings for confocal and > > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > > resources or existing quizzes that can ensure users know what they > > need to would also be helpful. > > Thanks, > > Niyanta > > > -- > ------------------------------------------------------------ > Steffen Dietzel, PD Dr. rer. nat > Ludwig-Maximilians-Universität München > Biomedical Center (BMC) > Head of the Core Facility Bioimaging > > Großhaderner Straße 9 > D-82152 Planegg-Martinsried > Germany > > http://www.bioimaging.bmc.med.uni-muenchen.de > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New > Jersey, USA 07033), and/or its affiliates Direct contact information for > affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, please > notify us immediately by reply e-mail and then delete it from your system. > > |
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Oshel, Philip Eugene |
Re: Confocal and Widefield microscopy training material
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In reply to this post by Phillips, Thomas E.
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We teach a grad-level microscopy course in confocal (also SEM & TEM courses). Tuition dollars don't come to the facility, but the instrument use (student use hours) is billed to the course, and that money does come to the facility. The instruments are billed at the standard "in-house" rate. Note: This are the same courses the undergrads in our "microscopy major" take. Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office -----Original Message----- From: Confocal Microscopy List <[hidden email]> on behalf of "Phillips, Thomas E." <[hidden email]> Reply-To: Confocal Microscopy List <[hidden email]> Date: Thursday, 17October, 2019 at 15:34 To: "[hidden email]" <[hidden email]> Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Does anybody offer their confocal training as an official grad-level class? In the past, offering a hands-on class was problematic for us since (i) no tuition dollars flow back to the core and (ii) PI's wanted their students using their own samples so "free class time" meant no income for us when those projects would normally have been done outside of class. Our campus is moving to a new budget model and tuition dollars may flow more directly back to us. We are considering combining training and supervised independent use as part of the class design and allow student efforts to be credentialed. I would be interested to know if anyone else is taking this approach and how many credit hours you offer and what you cover as part of the course. I have never met a PI who would have paid for Sylvie's design and impressed her clients do so. Thomas E. Phillips, Ph.D Professor of Biological Sciences Curators’ Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email] http://www.biology.missouri.edu/faculty/phillips.html http://microscopy.missouri.edu -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Kumar, Niyanta Sent: Thursday, October 17, 2019 2:12 PM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel Sent: Thursday, October 17, 2019 6:55 AM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material EXTERNAL EMAIL – Use caution with any links or file attachments. ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://www.bioimaging.bmc.med.uni-muenchen.de/learn/ Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany http://www.bioimaging.bmc.med.uni-muenchen.de Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. |
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Cammer, Michael |
Re: Confocal and Widefield microscopy training material
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I have pinch hit lectured and ran labs in many courses. Sometimes the instrument time was paid for out of an education budget, but mostly not. The core (or me personally since most lectures were prepared at home) absorbed the costs of my time. I also taught two years of a week-long course for new grad students. I sent bills, but don’t know if they were actually paid. Teaching does bring in business. Students who find our teaching useful or interesting spread the word. Cheers- Michael Cammer -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Oshel, Philip Eugene Sent: Thursday, October 17, 2019 3:47 PM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=AQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=Mal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE&e= and include the link in your posting. ***** We teach a grad-level microscopy course in confocal (also SEM & TEM courses). Tuition dollars don't come to the facility, but the instrument use (student use hours) is billed to the course, and that money does come to the facility. The instruments are billed at the standard "in-house" rate. Note: This are the same courses the undergrads in our "microscopy major" take. Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office -----Original Message----- From: Confocal Microscopy List <[hidden email]> on behalf of "Phillips, Thomas E." <[hidden email]> Reply-To: Confocal Microscopy List <[hidden email]> Date: Thursday, 17October, 2019 at 15:34 To: "[hidden email]" <[hidden email]> Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=AQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=Mal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE&e= and include the link in your posting. ***** Does anybody offer their confocal training as an official grad-level class? In the past, offering a hands-on class was problematic for us since (i) no tuition dollars flow back to the core and (ii) PI's wanted their students using their own samples so "free class time" meant no income for us when those projects would normally have been done outside of class. Our campus is moving to a new budget model and tuition dollars may flow more directly back to us. We are considering combining training and supervised independent use as part of the class design and allow student efforts to be credentialed. I would be interested to know if anyone else is taking this approach and how many credit hours you offer and what you cover as part of the course. I have never met a PI who would have paid for Sylvie's design and impressed her clients do so. Thomas E. Phillips, Ph.D Professor of Biological Sciences Curators’ Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email] https://urldefense.proofpoint.com/v2/url?u=http-3A__www.biology.missouri.edu_faculty_phillips.html&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=2X2JzDknmfdEUySiYs80Xwo3E8_H7XOtsRvsqjyk-as&e= https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopy.missouri.edu&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=nH6sgQk_G8l8jC3aTU7fVEdy8GAniz_FC3AxsckNPMs&e= -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Kumar, Niyanta Sent: Thursday, October 17, 2019 2:12 PM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=AQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=Mal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE&e= and include the link in your posting. ***** Thank you Neftali, Kathy, Sylvie, and Steffen for the suggestions and excellent training resources to go through! Much appreciated 😊 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel Sent: Thursday, October 17, 2019 6:55 AM To: [hidden email] Subject: Re: Confocal and Widefield microscopy training material EXTERNAL EMAIL – Use caution with any links or file attachments. ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=AQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=Mal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE&e= and include the link in your posting. ***** Hi Niyanta, I have collected a few resources on our website, so that I can refer our users to it more easily. This is at https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_learn_&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=8ICIFmvnIJoBPS2mAi7Ci-kXZCRIY5Bv8knrcEHDEtQ&e= Have a look at "Educational Websites" and "Materials for teaching". Maybe you find something helpful. The listed page from Australia (was mentioned by others too) contains a quiz that you can take, and you can print out a certificate if you answer 8+ of 10 questions correct. As in terms of hands on training, we start with a 3 h introduction session for up to 3 people. Usually with our samples, for two reasons. First, we want the user to pay attention to the microscope and software, not on the biological content of the images we make. Second, this way we can be sure that the sample is all right and we don't have users with crappy samples blaming it on the microscope. I agree with others that after 3 hours it makes no more sense, brains are full. Next we do a 'guided session' with the user's sample that we accompany until the user is getting good results. This is 1:1. Depending on sample difficulty, prior knowledge and user talent we sometimes have a third session. I am impressed by Sylvie's approach, but I doubt our group leaders would be willing to come up with so much money for training. Some think what we do now is already too time consuming (but most are happy about it). Steffen Am 11.10.2019 um 21:59 schrieb Niyanta Kumar: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=AQLCeaeYBp3fMI64Q8_KMmqA91L28AULRu0baAJ5-t8&e= > Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=Mal1tpfrgyNu-bzfh_caZtNsrsMOt7Uu_6bIFRCTLYE&e= and include the link in your posting. > ***** > > Hi all, > Can someone suggest any existing resources and formats to pull from > for designing new and advanced user trainings for confocal and > widefield microscopy? I need to design trainings for a Zeiss LSM 980 > Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive > resources or existing quizzes that can ensure users know what they > need to would also be helpful. > Thanks, > Niyanta > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany https://urldefense.proofpoint.com/v2/url?u=http-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=XjRa4ovswLBxD4kAWsQfoP7jxzMCqpm5soZBwmqyrBQ&e= Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at https://urldefense.proofpoint.com/v2/url?u=http-3A__www.merck.com_contact_contacts.html&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=zfXsMaGbqz3ZfbprD6ERcEHgv8ySQDw5Q-JR8bVu8QY&s=xGrxuaqOzNwpRjABItP_H8wWQ5W5ecLG0QI24QEYPs8&e= ) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. |
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