Convenient sample for SHG
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Hello Lister,
Hope all are doing well. Can anyone please offer me suggestions for a handy sample for imaging SHG in transmission and/or reflection? Since I am not working in a lab anymore, something that I could pick up in store would be ideal. I know its a strange request but I beg your indulgence in this regard. Cheers Farid |
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There are biology shops which sell prepared slides for schools –
skin would be a good choice, as would tooth or bone. If you don’t
care what you are looking at and just want to see if you are detecting a
signal, anything containing starch will give a good signal. Flour, cornstarch,
laundry starch – they should all work. If you have any access to
simple chemicals, either of the sodium phosphates should be good and very
bright indeed.
Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351
7682
Mobile 0413 281 861 ______________________________________________ From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Farid Jalali Hello Lister, No virus
found in this incoming message. |
 
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George McNamara |
Re: Convenient sample for SHG
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In reply to this post by Farid Jalali
Hi Farid,
A MP/SHG contact at Leica recommended urea crystals for SHG signal for measuring the instrument response function of our FLIM component. I don't know if you can buy this at the store (maybe online at amazon.com?), but you could produce it yourself. Slides containing what Guy Cox suggested can probably be purchased from Carolina Biological Supply (www.carolina.com) or Amazon. best wishes, George At 04:11 PM 7/2/2010, you wrote: >Hello Lister, >Hope all are doing well. Can anyone please offer me suggestions for >a handy sample for imaging SHG in transmission and/or reflection? >Since I am not working in a lab anymore, something that I could pick >up in store would be ideal. I know its a strange request but I beg >your indulgence in this regard. >Cheers >Farid > |
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Boswell, Carl A - (cboswell) |
Re: Convenient sample for SHG
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In reply to this post by Farid Jalali
Quoting Farid Jalali <[hidden email]>:
> Hello Lister, > Hope all are doing well. Can anyone please offer me suggestions for a handy > sample for imaging SHG in transmission and/or reflection? Since I am not > working in a lab anymore, something that I could pick up in store would be > ideal. I know its a strange request but I beg your indulgence in this > regard. > Cheers > Farid Haven't tried it myself, but I've been told on good authority that a slice of potato (lots of starch) will demonstrate SHG. You can hardly get cheaper than that. I wonder if it still "performs" after it turns black from exposure to air. Carl Boswell |
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I dissolved some KDP or KTP in hot water and let it evaporate onto
slides and coverslips. The resulting crystals give spectacular SHG performance: http://www.ucalgary.ca/styslab/image Scroll down and click on 'Second Harmonic Generation Imaging' to see my results! The pictures were generated by imaging using a 60x or 40x oil lens through a standard coverslip with crystals grown on the backside using the evaporating method. Also, there are some pictures of rat tail collagen and dorsal column membrane with SHG signal. Craig On Sun, Jul 4, 2010 at 1:52 PM, Carl Boswell <[hidden email]> wrote: > Quoting Farid Jalali <[hidden email]>: > >> Hello Lister, >> Hope all are doing well. Can anyone please offer me suggestions for a >> handy >> sample for imaging SHG in transmission and/or reflection? Since I am not >> working in a lab anymore, something that I could pick up in store would be >> ideal. I know its a strange request but I beg your indulgence in this >> regard. >> Cheers >> Farid > > Haven't tried it myself, but I've been told on good authority that a slice > of > potato (lots of starch) will demonstrate SHG. You can hardly get cheaper > than > that. I wonder if it still "performs" after it turns black from exposure to > air. > > Carl Boswell > |
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It was pointed out I didn't explain my alphabet soup. KDP and KTP are
Potassium di- and tri-phosphate respectively. It usually comes in a powder. You can image the powder directly, but the grains are small. I prefer to dissolve the grains in hot water and grow larger crystals to get better pictures. Let the water evaporate on a coverslip, or wait for a supersaturated solution to cool; large crystals will precipitate out into solution. Craig On Sun, Jul 4, 2010 at 9:57 PM, Craig Brideau <[hidden email]> wrote: > I dissolved some KDP or KTP in hot water and let it evaporate onto > slides and coverslips. The resulting crystals give spectacular SHG > performance: > > http://www.ucalgary.ca/styslab/image > > Scroll down and click on 'Second Harmonic Generation Imaging' to see > my results! The pictures were generated by imaging using a 60x or 40x > oil lens through a standard coverslip with crystals grown on the > backside using the evaporating method. Also, there are some pictures > of rat tail collagen and dorsal column membrane with SHG signal. > > Craig > > > > On Sun, Jul 4, 2010 at 1:52 PM, Carl Boswell <[hidden email]> wrote: >> Quoting Farid Jalali <[hidden email]>: >> >>> Hello Lister, >>> Hope all are doing well. Can anyone please offer me suggestions for a >>> handy >>> sample for imaging SHG in transmission and/or reflection? Since I am not >>> working in a lab anymore, something that I could pick up in store would be >>> ideal. I know its a strange request but I beg your indulgence in this >>> regard. >>> Cheers >>> Farid >> >> Haven't tried it myself, but I've been told on good authority that a slice >> of >> potato (lots of starch) will demonstrate SHG. You can hardly get cheaper >> than >> that. I wonder if it still "performs" after it turns black from exposure to >> air. >> >> Carl Boswell >> > |
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Thanks very much to everyone for the replies. As always, much appreciated.
Best Farid On Mon, Jul 5, 2010 at 2:48 PM, Craig Brideau <[hidden email]> wrote: It was pointed out I didn't explain my alphabet soup. KDP and KTP are |
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Steffen Dietzel |
Re: Convenient sample for SHG
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As already suggested, we did successfully use potato slices. We
actually cut them with a microtome, got some looks :-), but every slice that is thin enough to allow forward collection should be good for the purpose. The signal is a pain to quantify, though, since no two starch granules are alike. If you need something more reproducible, you might want to disassemble a green laser pointer. I was told it contains a 1064 nm light source which is transformed by SHG through a crystal to 532 nm. Maybe you can isolate that crystal. According to Wikipedia it's a crystal of <http://en.wikipedia.org/wiki/Potassium_titanyl_phosphate>potassium titanyl phosphate (KTP) . http://en.wikipedia.org/wiki/Laser_pointer#Green_laser_pointer (If you do, let me know the result, sounds interesting). Good luck Steffen |
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Peter Gabriel Pitrone |
Re: Convenient sample for SHG
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In reply to this post by Farid Jalali
Hello Farid,
I'd like for you to try something for me... A gummy bear (or at least a very thin slice of one)! I'm sorry if this sounds crazy, but I really would like to know what happens. I can tell you that they are very birefringent, do to trial and error. Try putting one between crossed polarizers and start to squeeze its body for a good laugh, every kid I've shown it to gets a real good kick out of it... Pete On Jul 2, 2010, at 22:11 PM, Farid Jalali wrote: > Hello Lister, > Hope all are doing well. Can anyone please offer me suggestions for a handy sample for imaging SHG in transmission and/or reflection? Since I am not working in a lab anymore, something that I could pick up in store would be ideal. I know its a strange request but I beg your indulgence in this regard. > Cheers > Farid > > |
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Birefringent... gummy bears!? Will the wonders of optics never cease? @:-)
It makes sense if you think about it though; it's a gel (sort-of) so if you put stress on its structure one way or another you will get an order preference. Regarding yanking crystals out of laser pointers, that's a good way to get a cheap doubling crystal, but if you just want a test sample my 'grow crystals on a coverslip' method is a lot less work then trying to pry the tiny dot of crystal out of one of those pointers. KTP, KDP, and BBO are all good doublers. I use a commercial BBO crystal that cost about a grand ($1k) to double our Ti:Saph now and again. We do fluorescence spectroscopy with it. By tuning the Ti:Saph and doubling it I can get ~350-520nm light. The key thing about the BBO crystal is it is optimal for this kind of work so I get a pretty good conversion efficiency out of it. A 'scrounged' crystal may not work as well. Craig On Thu, Jul 8, 2010 at 9:52 AM, Peter Pitrone <[hidden email]> wrote: > Hello Farid, > > I'd like for you to try something for me... A gummy bear (or at least a very thin slice of one)! I'm sorry if this sounds crazy, but I really would like to know what happens. I can tell you that they are very birefringent, do to trial and error. Try putting one between crossed polarizers and start to squeeze its body for a good laugh, every kid I've shown it to gets a real good kick out of it... > > Pete > > On Jul 2, 2010, at 22:11 PM, Farid Jalali wrote: > >> Hello Lister, >> Hope all are doing well. Can anyone please offer me suggestions for a handy sample for imaging SHG in transmission and/or reflection? Since I am not working in a lab anymore, something that I could pick up in store would be ideal. I know its a strange request but I beg your indulgence in this regard. >> Cheers >> Farid >> >> > |
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