Cooling Hamamatsu ORCA Flash 4.0

[Kellie Beicker]

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Hi all,  

We have a Hamamatsu ORCA Flash 4.0 (C11440-22C) that is currently mounted on
to a combined microscope and AFM setup.  The on-board fan creates too much
noise in both fluorescence imaging and AFM force data so we would like to
cool the camera with either water cooling or forced air cooling.

I am curious if anyone has done this and has knowledge of the nuances of
this process.  For water cooling: What type of refrigerated circulation unit
was used? At what temperature was the system was operated? Did you use water
or antifreeze? etc.  Any possible details that may not be specifically
stated in the manual or that you learned from cooling your camera would be
appreciated.

Additionally, the AFM support recommended forced air cooling for the
quietest data acquisition, but I have even less information on this.  So if
anyone has air cooled this type of camera or similar, I would appreciate any
details.

Thank you in advance for your advice,
-Kellie

----------------------------------------
Kellie Beicker

Physics Research Assistant
Physics & Astronomy, CB 3255
The University of North Carolina at Chapel Hill
Email: [hidden email]

[Romin, Yevgeniy/Sloan Kettering Institute]

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Hi Kellie

This isn't exactly the answer to your question, but I feel you may benefit from me sharing my experiences, since we run into the problem of noise in the AFM force data as well.  We also have an AFM that is coupled to a microscope.  We discovered that the less things are plugged into the system while the AFM is imaging, the better results we get, since we eliminate as many potential noise sources as possible.  We usually use our camera to find and focus on the are of interest, but before beginning our force measurements we turn off the cameras and unplug them, as well as turn off all the fluorescent and transmitted light sources.  This is probably not optimal in your case, but that is how we dealt with this problem.  It just seems to me that any sort of forced air cooling may still introduce noise in the force measurement data.

Hope this helps at least somewhat

Yevgeniy
________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Kellie Beicker [[hidden email]]
Sent: Thursday, June 12, 2014 12:48 PM
To: [hidden email]
Subject: Cooling Hamamatsu ORCA Flash 4.0

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Hi all,

We have a Hamamatsu ORCA Flash 4.0 (C11440-22C) that is currently mounted on
to a combined microscope and AFM setup.  The on-board fan creates too much
noise in both fluorescence imaging and AFM force data so we would like to
cool the camera with either water cooling or forced air cooling.

I am curious if anyone has done this and has knowledge of the nuances of
this process.  For water cooling: What type of refrigerated circulation unit
was used? At what temperature was the system was operated? Did you use water
or antifreeze? etc.  Any possible details that may not be specifically
stated in the manual or that you learned from cooling your camera would be
appreciated.

Additionally, the AFM support recommended forced air cooling for the
quietest data acquisition, but I have even less information on this.  So if
anyone has air cooled this type of camera or similar, I would appreciate any
details.

Thank you in advance for your advice,
-Kellie

----------------------------------------
Kellie Beicker

Physics Research Assistant
Physics & Astronomy, CB 3255
The University of North Carolina at Chapel Hill
Email: [hidden email]


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[Glenn Merrill-Skoloff]

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I liquid cool mine using a Julabo F 25 circulating cooler set to 15°C. I do use an antifreeze (Julabo Thermal ED) diluted 1:10 in water but I could probably run it with just water.

— Glenn

Glenn Merrill-Skoloff
Division of Hemostasis and Thrombosis
Laboratory Manager




From: Yevgeniy Romin <[hidden email]<mailto:[hidden email]>>
Reply-To: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>>
Date: Thursday, June 12, 2014 at 1:51 PM
To: "[hidden email]<mailto:[hidden email]>" <[hidden email]<mailto:[hidden email]>>
Subject: Re: Cooling Hamamatsu ORCA Flash 4.0

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Hi Kellie

This isn't exactly the answer to your question, but I feel you may benefit from me sharing my experiences, since we run into the problem of noise in the AFM force data as well.  We also have an AFM that is coupled to a microscope.  We discovered that the less things are plugged into the system while the AFM is imaging, the better results we get, since we eliminate as many potential noise sources as possible.  We usually use our camera to find and focus on the are of interest, but before beginning our force measurements we turn off the cameras and unplug them, as well as turn off all the fluorescent and transmitted light sources.  This is probably not optimal in your case, but that is how we dealt with this problem.  It just seems to me that any sort of forced air cooling may still introduce noise in the force measurement data.

Hope this helps at least somewhat

Yevgeniy
________________________________________
From: Confocal Microscopy List [[hidden email]<mailto:[hidden email]>] on behalf of Kellie Beicker [[hidden email]<mailto:[hidden email]>]
Sent: Thursday, June 12, 2014 12:48 PM
To: [hidden email]<mailto:[hidden email]>
Subject: Cooling Hamamatsu ORCA Flash 4.0

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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*****

Hi all,

We have a Hamamatsu ORCA Flash 4.0 (C11440-22C) that is currently mounted on
to a combined microscope and AFM setup.  The on-board fan creates too much
noise in both fluorescence imaging and AFM force data so we would like to
cool the camera with either water cooling or forced air cooling.

I am curious if anyone has done this and has knowledge of the nuances of
this process.  For water cooling: What type of refrigerated circulation unit
was used? At what temperature was the system was operated? Did you use water
or antifreeze? etc.  Any possible details that may not be specifically
stated in the manual or that you learned from cooling your camera would be
appreciated.

Additionally, the AFM support recommended forced air cooling for the
quietest data acquisition, but I have even less information on this.  So if
anyone has air cooled this type of camera or similar, I would appreciate any
details.

Thank you in advance for your advice,
-Kellie

----------------------------------------
Kellie Beicker

Physics Research Assistant
Physics & Astronomy, CB 3255
The University of North Carolina at Chapel Hill
Email: [hidden email]<mailto:[hidden email]>


     =====================================================================



     Please note that this e-mail and any files transmitted from

     Memorial Sloan-Kettering Cancer Center may be privileged, confidential,

     and protected from disclosure under applicable law. If the reader of

     this message is not the intended recipient, or an employee or agent

     responsible for delivering this message to the intended recipient,

     you are hereby notified that any reading, dissemination, distribution,
     copying, or other use of this communication or any of its attachments

     is strictly prohibited.  If you have received this communication in

     error, please notify the sender immediately by replying to this message

     and deleting this message, any attachments, and all copies and backups

     from your computer.


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This message is intended for the use of the person(s) to whom it may be addressed. It may contain information that is privileged, confidential, or otherwise protected from disclosure under applicable law. If you are not the intended recipient, any dissemination, distribution, copying, or use of this information is prohibited. If you have received this message in error, please permanently delete it and immediately notify the sender. Thank you.

[mhobson]

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Hello Kellie,

Here are the recommendations for cooling the ORCA-Flash4.0 camera from
Hamamatsu's Technical Support team:

We recommend using soft water (but not deionized water) for cooling.

No need to use any anti bacterial agent, just change the cooling water every
3 months and flush out the system every 6 months.

Anti-freeze is not recommended (or necessary) due to the possibility that it
could corrode the internal water jacket.

Set the cooling temperature of the water to +20 degrees C.

The minimum flow rate should be 1 L/minute.

The following two chillers have been qualified by Hamamatsu to meet the
cooling requirements:

Julabo HF25-ED
Thermocube 400W

If you have additional questions please feel free to contact us directly.

Best,
Mark Hobson
Marketing Manager, Scientific Cameras
Hamamatsu Corporation

[Kevin Ryan]

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Old-school and _extremely_ crude, but in past labs I've seen forced air cooling work well if the fan is mounted remotely (off the vibration-isolated table), with the airflow directed to the camera via a suspended length of dryer hose. Duct tape may (ahem) have been involved to force the airflow through the camera case. The first time I saw this was with an intensified vidicon camera, and the cooling was sufficient to keep the black-level and gain constant on a strictly analog device not intended for microscopy.

The dryer hose isolates the microscope from any fan vibrations, and the setup can provide consistent if limited cooling.

Kevin Ryan
Senior Project Manager
Media Cybernetics, Inc.


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kellie Beicker
Sent: Thursday, June 12, 2014 12:48 PM
To: [hidden email]
Subject: Cooling Hamamatsu ORCA Flash 4.0

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi all,  

We have a Hamamatsu ORCA Flash 4.0 (C11440-22C) that is currently mounted on to a combined microscope and AFM setup.  The on-board fan creates too much noise in both fluorescence imaging and AFM force data so we would like to cool the camera with either water cooling or forced air cooling.

I am curious if anyone has done this and has knowledge of the nuances of this process.  For water cooling: What type of refrigerated circulation unit was used? At what temperature was the system was operated? Did you use water or antifreeze? etc.  Any possible details that may not be specifically stated in the manual or that you learned from cooling your camera would be appreciated.

Additionally, the AFM support recommended forced air cooling for the quietest data acquisition, but I have even less information on this.  So if anyone has air cooled this type of camera or similar, I would appreciate any details.

Thank you in advance for your advice,
-Kellie

----------------------------------------
Kellie Beicker

Physics Research Assistant
Physics & Astronomy, CB 3255
The University of North Carolina at Chapel Hill
Email: [hidden email]