Create a "gel" mixture of different indexes of refraction

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Marc Reinig Marc Reinig
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Create a "gel" mixture of different indexes of refraction

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I want to create a mixture of materials with different indexes of refraction to be used in testing adaptive optics performance.  Ideally, they should be compatible with each other (i.e. not oil and water) and without strong refractive edges like glass beads.

Is there a gel or some material I can vary the refractive index of and then layer and stir slightly to mix without homogenizing the solution and let it set so the various swirls would retain some integrity?

Thanks in advance,

Marco
Marc Reinig
Keck Center for Adaptive Optical Microscopy
Martin Wessendorf-2 Martin Wessendorf-2
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Re: Create a "gel" mixture of different indexes of refraction

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Dear Marco--

Different concentrations of sucrose have different indices of
refraction--80% sucrose in water has a refractive index of about 1.5.  
Perhaps you could simply mix them with gelatin to get the effect you're
looking for.

Good luck!

Martin Wessendorf



On 2/3/2017 4:56 PM, Marc Reinig wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I want to create a mixture of materials with different indexes of refraction to be used in testing adaptive optics performance.  Ideally, they should be compatible with each other (i.e. not oil and water) and without strong refractive edges like glass beads.
>
> Is there a gel or some material I can vary the refractive index of and then layer and stir slightly to mix without homogenizing the solution and let it set so the various swirls would retain some integrity?
>
> Thanks in advance,
>
> Marco
> Marc Reinig
> Keck Center for Adaptive Optical Microscopy
Steffen Dietzel Steffen Dietzel
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Re: Create a "gel" mixture of different indexes of refraction

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How about if you cast a really bad agarose gel? The type of thing the
students are warned to use because the DNA might get different speeds in
different lanes, not thoroughly heated in the microwave, with 'lenses'
inside. If you have a molecular biology lab nearby, they could make you
one and then you could cut out the interesting, messed up areas. You
also could mix different concentrations shortly before they become solid.

You probably already thought of (and discarded the idea of) a biological
sample, right?

Steffen


Am 03.02.2017 um 23:56 schrieb Marc Reinig:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I want to create a mixture of materials with different indexes of refraction to be used in testing adaptive optics performance.  Ideally, they should be compatible with each other (i.e. not oil and water) and without strong refractive edges like glass beads.
>
> Is there a gel or some material I can vary the refractive index of and then layer and stir slightly to mix without homogenizing the solution and let it set so the various swirls would retain some integrity?
>
> Thanks in advance,
>
> Marco
> Marc Reinig
> Keck Center for Adaptive Optical Microscopy
>

--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de
jan soetaert jan soetaert
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Re: Create a "gel" mixture of different indexes of refraction

In reply to this post by Marc Reinig
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further to the agarose gel idea, you can buy or make gradient gels agarose or acrylamide with increased density top to bottom