Marc Reinig |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I want to create a mixture of materials with different indexes of refraction to be used in testing adaptive optics performance. Ideally, they should be compatible with each other (i.e. not oil and water) and without strong refractive edges like glass beads. Is there a gel or some material I can vary the refractive index of and then layer and stir slightly to mix without homogenizing the solution and let it set so the various swirls would retain some integrity? Thanks in advance, Marco Marc Reinig Keck Center for Adaptive Optical Microscopy |
Martin Wessendorf-2 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear Marco-- Different concentrations of sucrose have different indices of refraction--80% sucrose in water has a refractive index of about 1.5. Perhaps you could simply mix them with gelatin to get the effect you're looking for. Good luck! Martin Wessendorf On 2/3/2017 4:56 PM, Marc Reinig wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > I want to create a mixture of materials with different indexes of refraction to be used in testing adaptive optics performance. Ideally, they should be compatible with each other (i.e. not oil and water) and without strong refractive edges like glass beads. > > Is there a gel or some material I can vary the refractive index of and then layer and stir slightly to mix without homogenizing the solution and let it set so the various swirls would retain some integrity? > > Thanks in advance, > > Marco > Marc Reinig > Keck Center for Adaptive Optical Microscopy |
Steffen Dietzel |
In reply to this post by Marc Reinig
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** How about if you cast a really bad agarose gel? The type of thing the students are warned to use because the DNA might get different speeds in different lanes, not thoroughly heated in the microwave, with 'lenses' inside. If you have a molecular biology lab nearby, they could make you one and then you could cut out the interesting, messed up areas. You also could mix different concentrations shortly before they become solid. You probably already thought of (and discarded the idea of) a biological sample, right? Steffen Am 03.02.2017 um 23:56 schrieb Marc Reinig: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > I want to create a mixture of materials with different indexes of refraction to be used in testing adaptive optics performance. Ideally, they should be compatible with each other (i.e. not oil and water) and without strong refractive edges like glass beads. > > Is there a gel or some material I can vary the refractive index of and then layer and stir slightly to mix without homogenizing the solution and let it set so the various swirls would retain some integrity? > > Thanks in advance, > > Marco > Marc Reinig > Keck Center for Adaptive Optical Microscopy > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany http://www.bioimaging.bmc.med.uni-muenchen.de |
jan soetaert |
In reply to this post by Marc Reinig
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** further to the agarose gel idea, you can buy or make gradient gels agarose or acrylamide with increased density top to bottom |
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