Cryogenic properties of fluorophores

[Rickman, Colin]

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Hi

I have been searching online looking for papers that have studied the fluorescent properties of fluorescent proteins and organic dyes. I haven’t managed to find much and wondered if anybody on this list has tried to do these experiments or is aware of any publications.

Regards

Colin


Dr Colin Rickman
Institute of Biological Chemistry, Biophysics and Bioengineering
School of Engineering and Physical Sciences
Heriot-Watt University
Edinburgh
EH14 4AS

Tel: [phone] (Office)

http://www.ib3.eps.hw.ac.uk
http://www.esric.org




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[John Oreopoulos]

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Hi Colin,

I believe W. E. Moerner did quite a bit of the pioneering work in the imaging and spectroscopy of fluorescent molecules at low temperatures, which played a big role in development of optical single-moleucule spectroscopy and microscopy as an entire research field. Here's his website:

http://www.stanford.edu/group/moerner/sms_gen.html

I would try looking at his selected publication list at the end. I think there might be some relevant information that you're seeking. In particular, these publications stand out:

W. E. Moerner, "Single-Molecule Optical Spectroscopy of Autofluorescent Proteins," J. Chem. Phys. 117, 10925 (2002).

W. E. Moerner, “Single-Molecule Optical Spectroscopy and Imaging: From Early Steps to Recent Advances,” in Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium 138, Springer Series in Chemical Physics Vol. 96, A. Gräslund, R. Rigler, J. Widengren, Eds. ( Springer-Verlag, Berlin, 2009).

Cheers,


John Oreopoulos
Staff Scientist
Spectral Applied Research Inc.
A Division of Andor Technology
Richmond Hill, Ontario
Canada
www.spectral.ca


On 2013-11-29, at 9:46 AM, Rickman, Colin wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> I have been searching online looking for papers that have studied the fluorescent properties of fluorescent proteins and organic dyes. I haven’t managed to find much and wondered if anybody on this list has tried to do these experiments or is aware of any publications.
>
> Regards
>
> Colin
>
>
> Dr Colin Rickman
> Institute of Biological Chemistry, Biophysics and Bioengineering
> School of Engineering and Physical Sciences
> Heriot-Watt University
> Edinburgh
> EH14 4AS
>
> Tel: [phone] (Office)
>
> http://www.ib3.eps.hw.ac.uk
> http://www.esric.org
>
>
>
>
> -----
> Sunday Times Scottish University of the Year 2011-2013
> Top in the UK for student experience
> Fourth university in the UK and top in Scotland (National Student Survey 2012)
>
> We invite research leaders and ambitious early career researchers to
> join us in leading and driving research in key inter-disciplinary themes.
> Please see www.hw.ac.uk/researchleaders for further information and how
> to apply.
>
> Heriot-Watt University is a Scottish charity
> registered under charity number SC000278.

[George McNamara]

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See:

High-resolution multiphoton cryomicroscopy.
<http://www.ncbi.nlm.nih.gov/pubmed/23867337>

König K, Uchugonova A, *Breunig* HG.

Methods. 2013 Jul 16. doi:pii: S1046-2023(13)00255-7.
10.1016/j.ymeth.2013.07.006. [Epub ahead of print]

PMID:
    23867337


Multiphoton imaging of freezing and heating effects in plant leaves.
<http://www.ncbi.nlm.nih.gov/pubmed/22987831>

*Breunig* HG, Tümer F, König K.

J Biophotonics. 2013 Aug;6(8):622-30. doi: 10.1002/jbio.201200093. Epub
2012 Sep 14.

PMID:
    22987831



Karsten Konig is a founder of JenLab -
http://www.jenlab.de/2PM-Cryo.133.0.html?&L=en 
<http://www.jenlab.de/2PM-Cryo.133.0.html?&L=en>

The manufacturer of the freezing unit is described in the papers above.
Would work for 1-photon fluorescence as well.


    CryoSTED microscopy - a new spectroscopic approach for improving the
    resolution of STED microscopy using low temperature

    Giske, Arnold
    http://archiv.ub.uni-heidelberg.de/volltextserver/7969/


When cold enough, I believe the shape of the fluorescence spectra become
much more structured, which could have advantages for multiplexing. See
Giske dissertation for more on cold photophysics.

Enjoy,

George


On 11/29/2013 8:46 AM, Rickman, Colin wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> I have been searching online looking for papers that have studied the fluorescent properties of fluorescent proteins and organic dyes. I haven’t managed to find much and wondered if anybody on this list has tried to do these experiments or is aware of any publications.
>
> Regards
>
> Colin
>
>
> Dr Colin Rickman
> Institute of Biological Chemistry, Biophysics and Bioengineering
> School of Engineering and Physical Sciences
> Heriot-Watt University
> Edinburgh
> EH14 4AS
>
> Tel: [phone] (Office)
>
> http://www.ib3.eps.hw.ac.uk
> http://www.esric.org
>
>
>
>
> -----
> Sunday Times Scottish University of the Year 2011-2013
> Top in the UK for student experience
> Fourth university in the UK and top in Scotland (National Student Survey 2012)
>
> We invite research leaders and ambitious early career researchers to
> join us in leading and driving research in key inter-disciplinary themes.
> Please see www.hw.ac.uk/researchleaders for further information and how
> to apply.
>
> Heriot-Watt University is a Scottish charity
> registered under charity number SC000278.
>
>    

--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/26/

[Stanislav Vitha-2]

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Just a qualitative observation:

When doing standard wide-field fluorescence microscopy at liquid nitrogen
temperature, I noticed that there was much less photobleaching of GFP
than at room temperature.  





Stan Vitha

Microscopy and Imaging Center

Texas A&M University