Custom cell culture media?

[Jennifer Waters]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I'm looking to have some DMEM made without riboflavin & folic acid.  We'd like to add small amounts of these fluorescent components to the media, to try to find a level that keeps cells happy while minimizing background for live cell imaging.  Has anyone tried this?  I know I could make it myself by ordering all of the individual components (ugh), and I know that Invitrogen will do this for large batches (10L).  Does anyone know of a company that will make smaller batches of custom media?

Thanks, Jennifer

--
Jennifer Waters, Ph.D.
Director, Nikon Imaging Center at Harvard Medical School

[Andrew Resnick]

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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I'm interested in this exact item, myself, for the same reason.  I
have been unable to locate either a supplier or a manufacturer, and
would appreciate any information as well!


Andy


At 12:02 PM 1/14/2008, you wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>I'm looking to have some DMEM made without riboflavin & folic
>acid.  We'd like to add small amounts of these fluorescent
>components to the media, to try to find a level that keeps cells
>happy while minimizing background for live cell imaging.  Has anyone
>tried this?  I know I could make it myself by ordering all of the
>individual components (ugh), and I know that Invitrogen will do this
>for large batches (10L).  Does anyone know of a company that will
>make smaller batches of custom media?
>
>Thanks, Jennifer
>
>--
>Jennifer Waters, Ph.D.
>Director, Nikon Imaging Center at Harvard Medical School

Andrew Resnick, Ph. D.
Instructor
Department of Physiology and Biophysics
Case Western Reserve University
216-368-6899 (V)
216-368-4223 (F)

[Julio Vazquez]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

-

Jennifer, 

Lonza makes custom media, but I don't know the quantity limits:

http://www.lonzabioscience.com/content/Bioscience/LiquidMedia.asp

Atlanta Biologicals makes custom media down to six liter batches:

http://www.atlantabio.com/custom.asp

and a Google search for [custom cell culture media] will give several additional hits...

Your Institution (or other local academia/biotechs) must have cell culture facilities... have you tried asking them?

Sorry I don't have anything more helpful...

--

Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

http://www.fhcrc.org/

=

On Jan 14, 2008, at 9:02 AM, Jennifer Waters wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I'm looking to have some DMEM made without riboflavin & folic acid.  We'd like to add small amounts of these fluorescent components to the media, to try to find a level that keeps cells happy while minimizing background for live cell imaging.  Has anyone tried this?  I know I could make it myself by ordering all of the individual components (ugh), and I know that Invitrogen will do this for large batches (10L).  Does anyone know of a company that will make smaller batches of custom media?

Thanks, Jennifer

--
Jennifer Waters, Ph.D.
Director, Nikon Imaging Center at Harvard Medical School

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Jennifer-
Atlanta Biologicals will also do it. THey probably all want to make a
large batch, but it comes as a powder.  It is hard to make small
batches because many of the components are at very low dilution in
media.

Carol Heckman

[Mancini, Michael A]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

               
Dear microscopists,

For those near enough to Houston to consider attending, here is a notice for next week's, free, one-day high throughput-imaging-based symposium at Baylor College of Medicine (Jan 28, 2008).

Registration is free, but required; please follow this link to register:

     http://www.accelrys.com/events/seminars/gcc

=========================================================

High Throughput Systems Biology: 
Drug Screening and Applications for Personalized Medicine


        Agenda
       
        10:00 am
        Morning Session
        Session Chair: Michael Mancini, Baylor College of Medicine
        Overview: Studying systems biology at the single cell level


        10:10 am
        Massively Parallel High Content Signal Transduction Analysis
        John Westwick, Odyssey Thera
       

        10:40 am
        Novel Approaches to High Content Analysis Using Live Cells
        David Andrews, McMaster University
       

        11:10 am
        Direct High Throughput Systems Level Analyses of Wild Type and Clinical Mutations of the Androgen Receptor
        Adam Szafran, Mancini Lab, Baylor College of Medicine
       

        11:40 am
        Cellular Systems Biology Applied to Drug Discovery and Drug Development
        Lans Taylor, Cellumen Inc
               

        12:10 pm
        LUNCH
       
        1:10 pm
        Afternoon Session
        Session Chair: Peter J. Davies, UTHSC-Houston
        Overview: High throughput systems biology in the Texas Medical Center and the John S. Dunn Gulf Coast Consortium for Chemical Genomics

        1:15 pm
        Advances in High Throughput HCS Instrumentation/Repository and Multi-Compartment Image Cytometry Software for Systems Biology
        Jeff Price, Burnham Institute
       
        1:45 pm
        Automated Proteome-Wide Determination and Modeling of Subcellular Location for Systems Biology
        Robert Murphy, Carnegie Mellon
               
        2:15 pm
        Enhanced Retroviral RM Mutagen (ERM) Technology and Genome-Wide Interaction Screens
        Songyang Zhou, Baylor College of Medicine
       
        2:45 pm
        Automated Systems Biology BioInformatics
        Tim Moran and Nancy Latimer, Accelrys


        3:15 pm
        Coffee Break
       

        3:30 pm
        Combining High Content Confocal Microscopy with High Throughput Screening
        Jim Borree, Evotec/Perkin-Elmer


        4:00 pm
        Functional Profiling of Molecular Libraries Against Biological Space
        Daniel R. Rines, Genomics Institute of the Novartis Research Foundation
       

        4:15 pm
        Round Table Discussion with Speakers
        Moderator: David Tweardy, MD, ad interim Chair, Department of Medicine, Baylor College of Medicine





=========================
Michael A. Mancini, Ph.D.
Associate Professor
Director, Integrated Microscopy Core
Co-Director, Gulf Coast Consortium for Chemical Genomics
Department of Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX  77030
713 798 8952 voice
713 798 3017 fax
713 408 0179 cell
[hidden email]