Data comparison: Leica SP2 - SP5

[Tino Jäger]

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As one of the features of the Leica SP5, the SNR seems to be better then
in the SP2 due to cooled PMTs.
My question: Is it possible to compare or even pool data of fine
structures like dendritic spines (volume etc) obtained from both systems
- or would you assume the results to be different under the same
experimental conditions?
Thanks!

--
Dr. Tino Jäger
Department of Neurophysiology
Center for Brain Research
Medical University of Vienna
Spitalgasse 4, A-1090 Vienna

Tel:  [phone]
Secr: [phone]
FAX:  [phone]

http://www.meduniwien.ac.at

[Nuno Moreno]

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Hi. As far as I know the major issue is not cooling but better AMPS.

Tino Jäger wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> As one of the features of the Leica SP5, the SNR seems to be better then
> in the SP2 due to cooled PMTs.
> My question: Is it possible to compare or even pool data of fine
> structures like dendritic spines (volume etc) obtained from both systems
> - or would you assume the results to be different under the same
> experimental conditions?
> Thanks!
>

[Guy Cox]

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Signal to noise is not likely to make results quantitatively different
but it could affect accuracy.

Wouldn't the best approach be to run the same sample (and protocols)
on both systems and compare?

                                                 Guy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Tino Jäger
Sent: Wednesday, 14 May 2008 5:59 PM
To: [hidden email]
Subject: Data comparison: Leica SP2 - SP5

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

As one of the features of the Leica SP5, the SNR seems to be better then
in the SP2 due to cooled PMTs.
My question: Is it possible to compare or even pool data of fine
structures like dendritic spines (volume etc) obtained from both systems
- or would you assume the results to be different under the same
experimental conditions?
Thanks!

--
Dr. Tino Jäger
Department of Neurophysiology
Center for Brain Research
Medical University of Vienna
Spitalgasse 4, A-1090 Vienna

Tel:  [phone]
Secr: [phone]
FAX:  [phone]

http://www.meduniwien.ac.at

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