Demo lightsheet samples?

[Gary Laevsky]

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Hi All,

Does anyone know of, ie does it exist, a source for samples to use for
training/reference on a lightsheet?  Of the MuVi/Z.1/rotating variety, not
diSPIMish.

Kind of like using BPAE's for confocal ...?

And something more exciting than beads in a plug.

Thanks

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

[Andrew Shaw]

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We've used brine shrimp in the past. You can order them from Carolina Biological (no commercial interest).

[Steffen Dietzel]

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I have seen people using for that purpose a bunch of algae from the
nearest garden pond, embedded in low-melting point agarose. The
chloroplasts give a nice fluorescence, some of them are quite beautiful
and apart from the agarose no costs are involved. Results may vary
according to location and season. But in any case nature has a tendency
to be more interesting than beads.

Steffen


Am 21.06.2018 um 14:31 schrieb Gary Laevsky:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
>
> Does anyone know of, ie does it exist, a source for samples to use for
> training/reference on a lightsheet?  Of the MuVi/Z.1/rotating variety, not
> diSPIMish.
>
> Kind of like using BPAE's for confocal ...?
>
> And something more exciting than beads in a plug.
>
> Thanks
>

--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de

[Nikos Ekizoglou - Planelight]

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I totally agree with Steffen that a natural sample is much more
interesting than beads for training. Nevertheless if you want to check
the resolution of the system, especially on the z axis, you need a
certified reference. Since Light Sheet is so much sample preparation
related, on demos we always carry a beads sample to show the resolution
of the system, independent of the samples we measure.

Nikos


El 21/06/2018 a las 15:06, Steffen Dietzel escribió:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I have seen people using for that purpose a bunch of algae from the
> nearest garden pond, embedded in low-melting point agarose. The
> chloroplasts give a nice fluorescence, some of them are quite
> beautiful and apart from the agarose no costs are involved. Results
> may vary according to location and season. But in any case nature has
> a tendency to be more interesting than beads.
>
> Steffen
>
>
> Am 21.06.2018 um 14:31 schrieb Gary Laevsky:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your
>> posting.
>> *****
>>
>> Hi All,
>>
>> Does anyone know of, ie does it exist, a source for samples to use for
>> training/reference on a lightsheet?  Of the MuVi/Z.1/rotating
>> variety, not
>> diSPIMish.
>>
>> Kind of like using BPAE's for confocal ...?
>>
>> And something more exciting than beads in a plug.
>>
>> Thanks
>>

--
*Nikos Ekizoglou*

*Plane Light S.L.*
Edificio CTM, O-309
28053, Madrid
Tel. [phone]
/www.planelight.net
/


---
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[Raphael J]

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*****

If you are looking at a 3D complex sample, and have the correct cell line
you can still create some spheroids.
Get some 96 well plates, coat them with poly-HEMA, put 1000 fluorescent
cells in each well,, wait a couple of days to get a 400um sphere of cells,
fix, pipettes them and put them in agarose.

Those samples can really challenge light sheet microscopy and be a good
training on optimizing imaging parameters, but not every cell line can make
spheroids.

2018-06-22 8:43 GMT+01:00 Nikos Ekizoglou - PlaneLight <
[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I totally agree with Steffen that a natural sample is much more
> interesting than beads for training. Nevertheless if you want to check the
> resolution of the system, especially on the z axis, you need a certified
> reference. Since Light Sheet is so much sample preparation related, on
> demos we always carry a beads sample to show the resolution of the system,
> independent of the samples we measure.
>
> Nikos
>
>
> El 21/06/2018 a las 15:06, Steffen Dietzel escribió:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> I have seen people using for that purpose a bunch of algae from the
>> nearest garden pond, embedded in low-melting point agarose. The
>> chloroplasts give a nice fluorescence, some of them are quite beautiful and
>> apart from the agarose no costs are involved. Results may vary according to
>> location and season. But in any case nature has a tendency to be more
>> interesting than beads.
>>
>> Steffen
>>
>>
>> Am 21.06.2018 um 14:31 schrieb Gary Laevsky:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your
>>> posting.
>>> *****
>>>
>>> Hi All,
>>>
>>> Does anyone know of, ie does it exist, a source for samples to use for
>>> training/reference on a lightsheet?  Of the MuVi/Z.1/rotating variety,
>>> not
>>> diSPIMish.
>>>
>>> Kind of like using BPAE's for confocal ...?
>>>
>>> And something more exciting than beads in a plug.
>>>
>>> Thanks
>>>
>>>
> --
> *Nikos Ekizoglou*
>
> *Plane Light S.L.*
> Edificio CTM, O-309
> 28053, Madrid
> Tel. [phone]
> /www.planelight.net
> /
>
>
> ---
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> busca de virus.
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>