Kyle Michael Douglass |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi all, Would someone please explain the primary differences between Nikon's Lambda series and VC series of Plan Apochromat objectives? From their website, I understand that the Lambda series objectives have better transmission towards the red end of the spectrum than the VC series objectives. Are there any relative advantages of the VC series? Thanks! Kyle Dr. Kyle M. Douglass Postdoctoral Fellow The Laboratory of Experimental Biophysics EPFL, Lausanne, Switzerland +41 21 69 30556 (Office) |
PEARSON Matthew |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Kyle, As i understand it the Lambda series lenses only have axial chromatic correction down to 435nm whereas the VC (Violet Corrected) have chromatic correction down to 405nm. As you say i think the overall transmission across the spectrum is better in the lambda series. There may well be other differences. Regards, Matt ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. |
Sefic Svara, Diana |
In reply to this post by Kyle Michael Douglass
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Kyle, The main difference is in the range of the chromatic aberration corrections. Chromatic aberration is the inability of a lens to focus light of different wavelengths to the same convergence point. The Lambda series is corrected for blue to far red range 435-850nm while the VC (violet corrected) series corrects from UV to red 405-656nm. Diana -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Douglass Kyle Michael Sent: 27 February 2014 15:09 To: [hidden email] Subject: Difference between Nikon Plan Apo Lambda Series and VC Series ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi all, Would someone please explain the primary differences between Nikon's Lambda series and VC series of Plan Apochromat objectives? From their website, I understand that the Lambda series objectives have better transmission towards the red end of the spectrum than the VC series objectives. Are there any relative advantages of the VC series? Thanks! Kyle Dr. Kyle M. Douglass Postdoctoral Fellow The Laboratory of Experimental Biophysics EPFL, Lausanne, Switzerland +41 21 69 30556 (Office) |
Craig Brideau |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I have used the Lambda series 25x 1.1 NA with 375nm excitation and it actually worked fairly well. The same lens also works for two-photon all the way out to 1000 or so as well, as we do two-photon on the same system (A1) with a Chameleon. Craig On Thu, Feb 27, 2014 at 8:37 AM, Sefic Svara, Diana < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Kyle, > > The main difference is in the range of the chromatic aberration > corrections. Chromatic aberration is the inability of a lens to focus light > of different wavelengths to the same convergence point. > The Lambda series is corrected for blue to far red range 435-850nm while > the VC (violet corrected) series corrects from UV to red 405-656nm. > > Diana > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Douglass Kyle Michael > Sent: 27 February 2014 15:09 > To: [hidden email] > Subject: Difference between Nikon Plan Apo Lambda Series and VC Series > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi all, > Would someone please explain the primary differences between Nikon's > Lambda series and VC series of Plan Apochromat objectives? > > From their website, I understand that the Lambda series objectives have > better transmission towards the red end of the spectrum than the VC series > objectives. Are there any relative advantages of the VC series? > > Thanks! > Kyle > > Dr. Kyle M. Douglass > Postdoctoral Fellow > The Laboratory of Experimental Biophysics EPFL, Lausanne, Switzerland > +41 21 69 30556 (Office) > |
FLEMING Robert |
In reply to this post by Kyle Michael Douglass
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Kyle, The Nikon VC lens will be chromatically corrected at the Violet end (Violet Corrected), we have one on our EC1 confocal and it seems to do what it claims, I don't know how it compares with the lambda lenses, yours Bob Bob Fleming Bio-Imaging Facility Manager The Roslin Institute and R(D)SVS University of Edinburgh Easter Bush Campus EH25 9RG +44 131 651 9409 http://www.roslin.ed.ac.uk/ -- The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Douglass Kyle Michael Sent: 27 February 2014 15:09 To: [hidden email] Subject: Difference between Nikon Plan Apo Lambda Series and VC Series ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi all, Would someone please explain the primary differences between Nikon's Lambda series and VC series of Plan Apochromat objectives? From their website, I understand that the Lambda series objectives have better transmission towards the red end of the spectrum than the VC series objectives. Are there any relative advantages of the VC series? Thanks! Kyle Dr. Kyle M. Douglass Postdoctoral Fellow The Laboratory of Experimental Biophysics EPFL, Lausanne, Switzerland +41 21 69 30556 (Office) |
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