*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all: I have a very fundamental question. As a foreigner from non-English country, recently, I'm absolutely confused with the definitions of diffraction-limited and diffraction-unlimited. For example, it is well known that a focal spot by an objective has a lateral size of 200 nm and an axial size of 600 nm. If I can obtain a focal spot with a size of 100 nm, it is apparent that it has broken the diffractive limit. Therefore, should I call it a diffraction-limited focal spot, or a diffraction-unlimited one? Actually, some papers I have ever read use the former word, but on the other hand, there are some other persons who tend to use the latter one. So, which one is correct? Thank you very much! -- ------ Hao, Xiang Ph.D Candidate, State Key Laboratory of Modern Optical Instrumentation, Zhejiang University +86-571-8795-3975 |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Diffraction-unlimited refers to techniques, such as STED, STORM and PALM, where there is no absolute theoretical limit to the resolution attainable. Other super-resolution techniques, such as 4-pi and linear structured illumination, give improved resolution, below the Rayleigh limit, but are still diffraction limited in that there is a defined limit to the resolution improvement available. Of course even diffraction-unlimited techniques are ultimately limited by signal to noise ratios. Guy Optical Imaging Techniques in Cell Biology by Guy Cox 2nd edition, 2012 CRC Press http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Aust. Centre for Microscopy & Microanalysis, F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Hao,Xiang Sent: Thursday, 15 November 2012 4:03 PM To: [hidden email] Subject: Diffraction-limited or Diffraction-unlimited? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all: I have a very fundamental question. As a foreigner from non-English country, recently, I'm absolutely confused with the definitions of diffraction-limited and diffraction-unlimited. For example, it is well known that a focal spot by an objective has a lateral size of 200 nm and an axial size of 600 nm. If I can obtain a focal spot with a size of 100 nm, it is apparent that it has broken the diffractive limit. Therefore, should I call it a diffraction-limited focal spot, or a diffraction-unlimited one? Actually, some papers I have ever read use the former word, but on the other hand, there are some other persons who tend to use the latter one. So, which one is correct? Thank you very much! -- ------ Hao, Xiang Ph.D Candidate, State Key Laboratory of Modern Optical Instrumentation, Zhejiang University +86-571-8795-3975 |
Mark Cannell-2 |
In reply to this post by Xiang Hao
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Unfortunately, English is sometimes badly used and the introduction of the term "diffraction-unlimited" is an example. Whoever invented this term did not realise/know that the hyphen makes the term "diffraction-limited" effectively one word. This new word should then be modified as per the usual english usage which require the modifier in front. I think that what is/was meant is "sub-diffraction-limited" but better english would be to use negation as in "the spot size is not diffraction-limited" IMHO. You do not have to use incorrect constructions just because some else does. Cheers Mark On 15/11/2012, at 5:02 AM, "Hao,Xiang" <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear all: > I have a very fundamental question. As a foreigner from non-English > country, recently, I'm absolutely confused with the definitions of > diffraction-limited and diffraction-unlimited. > For example, it is well known that a focal spot by an objective has a > lateral size of 200 nm and an axial size of 600 nm. If I can obtain a focal > spot with a size of 100 nm, it is apparent that it has broken the > diffractive limit. Therefore, should I call it a diffraction-limited focal > spot, or a diffraction-unlimited one? Actually, some papers I have ever > read use the former word, but on the other hand, there are some other > persons who tend to use the latter one. So, which one is correct? > Thank you very much! > > -- > ------ > Hao, Xiang > Ph.D Candidate, > State Key Laboratory of Modern Optical Instrumentation, Zhejiang University > +86-571-8795-3975 Mark B. Cannell Ph.D. FRSNZ Professor of Cardiac Cell Biology School of Physiology& Pharmacology Medical Sciences Building University of Bristol Bristol BS8 1TD UK [hidden email] |
Free forum by Nabble | Edit this page |