Dipping lenses on an inverted scope
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Graham Wright-5 |
Dipping lenses on an inverted scope
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Hello,
Has anyone ever (successfully) developed a way of
using water dipping lenses on an inverted microscope. We were discussing the
options and practicalities of this (eg by having a rubber seal round the
objective with the sample stuck to a slide above immersed in
media).
Has it been done before, or is it just a rather
risky idea?
Thanks,
Graham
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Graham Wright Microscopy & Imaging Facility Temasek Life Sciences Laboratory
1 Research Link National University of Singapore Singapore 117604 P: +65 6872
8406 E: graham@... |
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Nowell, Cameron |
Re: Dipping lenses on an inverted scope
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Graham, I have seen a presentation by Stephen Cody of the Ludwig Cancer Centre (a regular on this list) that showed a dipping lens sealed to the bottom of a dish on an inverted microscope. I belive they used a condom to create the seal. Steve is currently away on holidays but he should be happy to help you when he gets back Cheers Cam Cameron Nowell B.Sc (Hons) Microscopy Imaging and Research Core Facility Peter MacCallum Cancer Centre 7 St Andrews Place East Melbourne, Victoria 3002 Phone: +61396561243 Mobile: +61422882700 Fax: +61396561411 ________________________________ From: Confocal Microscopy List on behalf of Graham Wright Sent: Mon 21/01/2008 9:36 PM To: [hidden email] Subject: Dipping lenses on an inverted scope Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello, Has anyone ever (successfully) developed a way of using water dipping lenses on an inverted microscope. We were discussing the options and practicalities of this (eg by having a rubber seal round the objective with the sample stuck to a slide above immersed in media). Has it been done before, or is it just a rather risky idea? Thanks, Graham ----- Graham Wright Microscopy & Imaging Facility Temasek Life Sciences Laboratory 1 Research Link National University of Singapore Singapore 117604 P: +65 6872 8406 E: [hidden email] This email (including any attachments) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. |
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lechristophe |
Re: Dipping lenses on an inverted scope
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In reply to this post by Graham Wright-5
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The simplest is to use a viscous medium that has a refractive index identical to the water, like the immersol W from Carl Zeiss :
http://tinyurl.com/33q487 Christophe Leterrier On Jan 21, 2008 11:36 AM, Graham Wright <[hidden email]> wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal |
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lechristophe |
Re: Dipping lenses on an inverted scope
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Sorry, I think I misread your post. Using Immersol W is usefull for water-immersion objectives designed to work with a coverslip, and you want a dipping objective.
Christophe On Jan 21, 2008 12:02 PM, Christophe Leterrier <
[hidden email]> wrote: The simplest is to use a viscous medium that has a refractive index identical to the water, like the immersol W from Carl Zeiss : |
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In reply to this post by Graham Wright-5
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My guess is that just about everyone has tried this.
Steve Cody did
clever things with condoms. What we did is build up a
dam with dental
rubber around the lens to hold the water. Took a bit
of trial and error
but it was very effective and cost very
little.
Guy
Optical Imaging Techniques in Cell Biology From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Graham Wright Sent: Monday, 21 January 2008 9:37 PM To: [hidden email] Subject: Dipping lenses on an inverted scope Hello,
Has anyone ever (successfully) developed a way of
using water dipping lenses on an inverted microscope. We were discussing the
options and practicalities of this (eg by having a rubber seal round the
objective with the sample stuck to a slide above immersed in
media).
Has it been done before, or is it just a rather
risky idea?
Thanks,
Graham
-----
Graham Wright Microscopy & Imaging Facility Temasek Life Sciences Laboratory
1 Research Link National University of Singapore Singapore 117604 P: +65 6872
8406 E: graham@... No virus found in this incoming message. No virus found in this outgoing message. |
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Peter Gabriel Pitrone |
Re: Dipping lenses on an inverted scope
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In reply to this post by Graham Wright-5
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Hello Graham, This objective inverter might work: No Commercial Interest (not even a costumer): But plenty of interest in great ideas! Pete On Jan 21, 2008, at 11:36 AM, Graham Wright wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Peter Gabriel Pitrone Light Microscopy Facility Max Planck Institute for Cell Biology and Genetics Pfotenhauerstrasse 108 01307 Dresden, Germany E-Mail: [hidden email] |
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Gert van Cappellen |
Re: Dipping lenses on an inverted scope
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In reply to this post by Graham Wright-5
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Good luck, Gert on 21-1-2008 11:36 Graham Wright said the following: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal -- wigGert van Cappellen, [hidden email] Optical Imaging Centre; http://www.erasmusmc.nl/oic/ Erasmus MC, room Ee914, Dr. Molenwaterplein 50, 3015 GE ROTTERDAM, The Netherlands |
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Stephen Cody |
Re: Dipping lenses on an inverted scope
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In reply to this post by Graham Wright-5
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Search the CONFOCAL archive at
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Dear Graham,
Thanks Cameron and Guy. Graham the reference to the dipping lens and condoms is:
CODY, S.H. & Williams, D.A. A novel organ bath design enables the use of saline-immersible lenses on inverted microscopes. Journal of Microscopy 185(1): 94-97, (1997). http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1365-2818.1997.1370697.x
I’ll send a PDF offline.
You may also want to have a look at the brief PowerPoint presentation on this subject which is available on our web page.
http://www.ludwig.edu.au/confocal/Condom.pps (If prompted for a password, hit the cancel button)
As Peter Pitrone has already suggested, I would also recommend a look at the Objective Inverter by LSMtech . I think this is a better solution to the problem than using condoms.
No commercial affiliation with LSMtech. I would like to acknowledge the generous donation of several packets of Non-lubricated condoms by Ansell. Funny and also true! Cheers Stephen H. Cody Tip: Learn how to receive reminders about you microscope
booking: -----Original Message-----
Hello, Has anyone ever (successfully) developed a way of using water dipping lenses on an inverted microscope. We were discussing the options and practicalities of this (eg by having a rubber seal round the objective with the sample stuck to a slide above immersed in media). Has it been done before, or is it just a rather risky idea? Thanks, Graham
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