Direct diode modulation v aotf
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Laevsky, Gary S. |
Direct diode modulation v aotf
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi All, It was always my impression that direct modulation of a diode shortened the lifetime of said diode, vs pumping it at some constant voltage and modulating through an aotf. Recently, I have been informed this is not the case, at least using the OBIS LX lasers. Not that I would ever doubt anything I've ever been told, but a supporting second opinion would be nice. Thanks. Best, Gary Gary Laevsky, Ph.D. Confocal Imaging Facility Manager Dept. of Molecular Biology Washington Rd. Princeton University Princeton, New Jersey, 08544-1014 (O) 609 258 5432 (C) 508 507 1310 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear All, I have just called Molecular Probes, and, shamefully, they told me that a table with photo-chemical properties of all Alexa dyes does not exist. I have seen one with lifetime and QY data of most of Alexa dyes, but not all. Would you know if someone carefully compared relative photo-stability of Alexa dyes (A488 vs A568 vs A594), and optionally would also include Cy-3B and other cyanide dyes. Best regards, Vitaly |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** From the Life Technologies website (these guys REALLY need to get their tech service in gear!) http://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/tables/fluorescence-quantum-yields-and-lifetimes-for-alexa-fluor-dyes.html ................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM ________________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Vitaly Boyko <[hidden email]> Sent: Thursday, October 17, 2013 1:13 PM To: [hidden email] Subject: Alexa dyes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear All, I have just called Molecular Probes, and, shamefully, they told me that a table with photo-chemical properties of all Alexa dyes does not exist. I have seen one with lifetime and QY data of most of Alexa dyes, but not all. Would you know if someone carefully compared relative photo-stability of Alexa dyes (A488 vs A568 vs A594), and optionally would also include Cy-3B and other cyanide dyes. Best regards, Vitaly |
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In reply to this post by Vitaly Boyko
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Following up, does anyone have a table of Alexa dye bleaching properties? I find they differ wildly in photostability. Rick Hallworth Creighton University IBIF |
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Kilgore, Jason-2 |
Re: Alexa dyes **vendor reply**
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In reply to this post by Tamara Howard
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** ** Vendor Reply ** Hi, Vitaly, In addition to the link that Tamara suggested for QY and lifetime data, you can find comparative photobleach data in the Molecular Probes Handbook, here (see Photobleaching section halfway down page): http://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/introduction-to-fluorescence-techniques.html There is also an excellent paper on Alexa Fluor dye photostability data, compared to other dyes (including Cy3) in a number of different mounting media: Panchuk-Voloshina N. et al. 1999. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem 47 (9): 1179-1188. Of course, photostability will vary depending on the light exposure, mountant, and other environmental factors. I hope this helps. Cheers, Jason Jason A. Kilgore Technical Application Scientist Molecular Probes Labeling and Detection Technologies Cells Systems Division T 1 800 955 6288 then option 4, then option 6, or 541 335 0353 * F 541 335 0238 29851 Willow Creek Rd * Eugene * OR * 97402-9132 * United States -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Tamara Howard Sent: Thursday, October 17, 2013 1:20 PM To: [hidden email] Subject: Re: Alexa dyes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** From the Life Technologies website (these guys REALLY need to get their tech service in gear!) http://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/tables/fluorescence-quantum-yields-and-lifetimes-for-alexa-fluor-dyes.html ................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM ________________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Vitaly Boyko <[hidden email]> Sent: Thursday, October 17, 2013 1:13 PM To: [hidden email] Subject: Alexa dyes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear All, I have just called Molecular Probes, and, shamefully, they told me that a table with photo-chemical properties of all Alexa dyes does not exist. I have seen one with lifetime and QY data of most of Alexa dyes, but not all. Would you know if someone carefully compared relative photo-stability of Alexa dyes (A488 vs A568 vs A594), and optionally would also include Cy-3B and other cyanide dyes. Best regards, Vitaly |
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I have seen everything what's published and what's available
***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Jason, I have seen everything what's published and what's available on the MolProbes website - the latter just confirms lack of professionalism at your company. Would you have any idea when your business would be up to PRO standards???!!! Best regards, Vitaly On Thursday, October 17, 2013 4:45 PM, "Kilgore, Jason" <[hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** ** Vendor Reply ** Hi, Vitaly, In addition to the link that Tamara suggested for QY and lifetime data, you can find comparative photobleach data in the Molecular Probes Handbook, here (see Photobleaching section halfway down page): http://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/introduction-to-fluorescence-techniques.html There is also an excellent paper on Alexa Fluor dye photostability data, compared to other dyes (including Cy3) in a number of different mounting media: Panchuk-Voloshina N. et al. 1999. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem 47 (9): 1179-1188. Of course, photostability will vary depending on the light exposure, mountant, and other environmental factors. I hope this helps. Cheers, Jason Jason A. Kilgore Technical Application Scientist Molecular Probes Labeling and Detection Technologies Cells Systems Division T 1 800 955 6288 then option 4, then option 6, or 541 335 0353 * F 541 335 0238 29851 Willow Creek Rd * Eugene * OR * 97402-9132 * United States -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Tamara Howard Sent: Thursday, October 17, 2013 1:20 PM To: [hidden email] Subject: Re: Alexa dyes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** From the Life Technologies website (these guys REALLY need to get their tech service in gear!) http://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/tables/fluorescence-quantum-yields-and-lifetimes-for-alexa-fluor-dyes.html ................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM ________________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Vitaly Boyko <[hidden email]> Sent: Thursday, October 17, 2013 1:13 PM To: [hidden email] Subject: Alexa dyes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear All, I have just called Molecular Probes, and, shamefully, they told me that a table with photo-chemical properties of all Alexa dyes does not exist. I have seen one with lifetime and QY data of most of Alexa dyes, but not all. Would you know if someone carefully compared relative photo-stability of Alexa dyes (A488 vs A568 vs A594), and optionally would also include Cy-3B and other cyanide dyes. Best regards, Vitaly |
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In reply to this post by Vitaly Boyko
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Vitaly, You brought up Cy3B - what is the GE web page with the quantum yield of Cy3B, please? If you think Molecular Probes current web site and support are bad -- in spite of Jason's reply here - you should be looking forward to the changes when Thermo Fisher buys Life Technologies/Invitrogen/Molecular Probes. Many extinction coefficients and quantum yields (not Alexa Fluor 488 however) are available in the PubSpectra XLSX file inside the zip download at http://works.bepress.com/gmcnamara/9/ The Excel file was the starting point for the U Arizona Spectra Database http://www.spectra.arizona.edu/ which has additional spectra, especially multiphoton excitation. another spectra viewer - and new dye that I learned from a Probes salesperson's visit today, is at http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html#product=S11200 George On 10/17/2013 2:13 PM, Vitaly Boyko wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear All, > > I have just called Molecular Probes, and, shamefully, they told me that a table with photo-chemical properties of all Alexa dyes does not exist. I have seen one with lifetime and QY data of most of Alexa dyes, but not all. > Would you know if someone carefully compared relative photo-stability of Alexa dyes (A488 vs A568 vs A594), and optionally would also include Cy-3B and other cyanide dyes. > > Best regards, > > Vitaly > > -- George McNamara, Ph.D. Single Cells Analyst L.J.N. Cooper Lab University of Texas M.D. Anderson Cancer Center Houston, TX 77054 http://works.bepress.com/gmcnamara/26/ |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Much of fluorescence microscopy is possible thanks to Molecular Probes. Even if they occasionally miss something. Mike ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of George McNamara [[hidden email]] Sent: Thursday, October 17, 2013 9:55 PM To: [hidden email] Subject: Re: Alexa dyes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Vitaly, You brought up Cy3B - what is the GE web page with the quantum yield of Cy3B, please? If you think Molecular Probes current web site and support are bad -- in spite of Jason's reply here - you should be looking forward to the changes when Thermo Fisher buys Life Technologies/Invitrogen/Molecular Probes. Many extinction coefficients and quantum yields (not Alexa Fluor 488 however) are available in the PubSpectra XLSX file inside the zip download at http://works.bepress.com/gmcnamara/9/ The Excel file was the starting point for the U Arizona Spectra Database http://www.spectra.arizona.edu/ which has additional spectra, especially multiphoton excitation. another spectra viewer - and new dye that I learned from a Probes salesperson's visit today, is at http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html#product=S11200 George On 10/17/2013 2:13 PM, Vitaly Boyko wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear All, > > I have just called Molecular Probes, and, shamefully, they told me that a table with photo-chemical properties of all Alexa dyes does not exist. I have seen one with lifetime and QY data of most of Alexa dyes, but not all. > Would you know if someone carefully compared relative photo-stability of Alexa dyes (A488 vs A568 vs A594), and optionally would also include Cy-3B and other cyanide dyes. > > Best regards, > > Vitaly > > -- George McNamara, Ph.D. Single Cells Analyst L.J.N. Cooper Lab University of Texas M.D. Anderson Cancer Center Houston, TX 77054 http://works.bepress.com/gmcnamara/26/ |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I presume "occasionally" was a joke. Though I would agree that many Alexa dyes are quite good. However, I would disagree with the lack of comparative photo-stability data of Alexa dyes - "business as usual". What is about ATTO dyes, those are German. Are there any data on their relative photo-stability? Best regards, Vitaly On Friday, October 18, 2013 7:13 AM, "MODEL, MICHAEL" <[hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Much of fluorescence microscopy is possible thanks to Molecular Probes. Even if they occasionally miss something. Mike ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of George McNamara [[hidden email]] Sent: Thursday, October 17, 2013 9:55 PM To: [hidden email] Subject: Re: Alexa dyes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Vitaly, You brought up Cy3B - what is the GE web page with the quantum yield of Cy3B, please? If you think Molecular Probes current web site and support are bad -- in spite of Jason's reply here - you should be looking forward to the changes when Thermo Fisher buys Life Technologies/Invitrogen/Molecular Probes. Many extinction coefficients and quantum yields (not Alexa Fluor 488 however) are available in the PubSpectra XLSX file inside the zip download at http://works.bepress.com/gmcnamara/9/ The Excel file was the starting point for the U Arizona Spectra Database http://www.spectra.arizona.edu/ which has additional spectra, especially multiphoton excitation. another spectra viewer - and new dye that I learned from a Probes salesperson's visit today, is at http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html#product=S11200 George On 10/17/2013 2:13 PM, Vitaly Boyko wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear All, > > I have just called Molecular Probes, and, shamefully, they told me that a table with photo-chemical properties of all Alexa dyes does not exist. I have seen one with lifetime and QY data of most of Alexa dyes, but not all. > Would you know if someone carefully compared relative photo-stability of Alexa dyes (A488 vs A568 vs A594), and optionally would also include Cy-3B and other cyanide dyes. > > Best regards, > > Vitaly > > -- George McNamara, Ph.D. Single Cells Analyst L.J.N. Cooper Lab University of Texas M.D. Anderson Cancer Center Houston, TX 77054 http://works.bepress.com/gmcnamara/26/ |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Just out of curiosity, is there really a recognized standard method (in a microscope setting) to quantify relative photo-stability of a fluorescent probe? As has been pointed out, and I would think as well, this kind of measurement would be dependent on the local chemical environment of the probe and the excitation wavelengths you use (and where they lie relative to the absorption curve). Doing the measurement in a spectrometer within a cuvette is not really what you're after, right? John Oreopoulos Staff Scientist Spectral Applied Research Richmond Hill, Ontario Canada www.spectral.ca On 2013-10-18, at 2:25 PM, Vitaly Boyko wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I presume "occasionally" was a joke. > Though I would agree that many Alexa dyes are quite good. However, I would disagree with the lack of comparative photo-stability data of Alexa dyes - "business as usual". > > What is about ATTO dyes, those are German. Are there any data on their relative photo-stability? > > Best regards, > > Vitaly > > > > > > On Friday, October 18, 2013 7:13 AM, "MODEL, MICHAEL" <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Much of fluorescence microscopy is possible thanks to Molecular Probes. Even if they occasionally miss something. > > Mike > ________________________________________ > > From: Confocal Microscopy List [[hidden email]] on behalf of George McNamara [[hidden email]] > Sent: Thursday, October 17, 2013 9:55 PM > To: [hidden email] > Subject: Re: Alexa dyes > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Vitaly, > > You brought up Cy3B - what is the GE web page with the quantum yield of > Cy3B, please? > > If you think Molecular Probes current web site and support are bad -- in > spite of Jason's reply here - you should be looking forward to the > changes when Thermo Fisher buys Life Technologies/Invitrogen/Molecular > Probes. > > Many extinction coefficients and quantum yields (not Alexa Fluor 488 > however) are available in the PubSpectra XLSX file inside the zip > download at > > http://works.bepress.com/gmcnamara/9/ > > The Excel file was the starting point for the U Arizona Spectra Database > http://www.spectra.arizona.edu/ > which has additional spectra, especially multiphoton excitation. > > another spectra viewer - and new dye that I learned from a Probes > salesperson's visit today, is at > http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html#product=S11200 > > > George > > On 10/17/2013 2:13 PM, Vitaly Boyko wrote: >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Dear All, >> >> I have just called Molecular Probes, and, shamefully, they told me that a table with photo-chemical properties of all Alexa dyes does not exist. I have seen one with lifetime and QY data of most of Alexa dyes, but not all. >> Would you know if someone carefully compared relative photo-stability of Alexa dyes (A488 vs A568 vs A594), and optionally would also include Cy-3B and other cyanide dyes. >> >> Best regards, >> >> Vitaly >> >> > > > -- > > > > George McNamara, Ph.D. > Single Cells Analyst > L.J.N. Cooper Lab > University of Texas M.D. Anderson Cancer Center > Houston, TX 77054 > http://works.bepress.com/gmcnamara/26/ |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I think the best quantitative analysis of fluorescence photobleaching in the context of microscopy may still be Hans Tanke's work published about 1997. PubMed for "HJ Tanke". There were two papers in Biophys J and one in Cytometry if I remember correctly. They certainly showed clearly the difference in behavior between free and protein-conjugated dyes. Iain PS these papers date back to the pre-Alexa Fluor era, but the principles are the same. Sent from my iPhone On Oct 18, 2013, at 12:24 PM, John Oreopoulos <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Just out of curiosity, is there really a recognized standard method (in a microscope setting) to quantify relative photo-stability of a fluorescent probe? As has been pointed out, and I would think as well, this kind of measurement would be dependent on the local chemical environment of the probe and the excitation wavelengths you use (and where they lie relative to the absorption curve). Doing the measurement in a spectrometer within a cuvette is not really what you're after, right? > > John Oreopoulos > Staff Scientist > Spectral Applied Research > Richmond Hill, Ontario > Canada > www.spectral.ca > > > On 2013-10-18, at 2:25 PM, Vitaly Boyko wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> I presume "occasionally" was a joke. >> Though I would agree that many Alexa dyes are quite good. However, I would disagree with the lack of comparative photo-stability data of Alexa dyes - "business as usual". >> >> What is about ATTO dyes, those are German. Are there any data on their relative photo-stability? >> >> Best regards, >> >> Vitaly >> >> >> >> >> >> On Friday, October 18, 2013 7:13 AM, "MODEL, MICHAEL" <[hidden email]> wrote: >> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Much of fluorescence microscopy is possible thanks to Molecular Probes. Even if they occasionally miss something. >> >> Mike >> ________________________________________ >> >> From: Confocal Microscopy List [[hidden email]] on behalf of George McNamara [[hidden email]] >> Sent: Thursday, October 17, 2013 9:55 PM >> To: [hidden email] >> Subject: Re: Alexa dyes >> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hi Vitaly, >> >> You brought up Cy3B - what is the GE web page with the quantum yield of >> Cy3B, please? >> >> If you think Molecular Probes current web site and support are bad -- in >> spite of Jason's reply here - you should be looking forward to the >> changes when Thermo Fisher buys Life Technologies/Invitrogen/Molecular >> Probes. >> >> Many extinction coefficients and quantum yields (not Alexa Fluor 488 >> however) are available in the PubSpectra XLSX file inside the zip >> download at >> >> http://works.bepress.com/gmcnamara/9/ >> >> The Excel file was the starting point for the U Arizona Spectra Database >> http://www.spectra.arizona.edu/ >> which has additional spectra, especially multiphoton excitation. >> >> another spectra viewer - and new dye that I learned from a Probes >> salesperson's visit today, is at >> http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html#product=S11200 >> >> >> George >> >> On 10/17/2013 2:13 PM, Vitaly Boyko wrote: >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Dear All, >>> >>> I have just called Molecular Probes, and, shamefully, they told me that a table with photo-chemical properties of all Alexa dyes does not exist. I have seen one with lifetime and QY data of most of Alexa dyes, but not all. >>> Would you know if someone carefully compared relative photo-stability of Alexa dyes (A488 vs A568 vs A594), and optionally would also include Cy-3B and other cyanide dyes. >>> >>> Best regards, >>> >>> Vitaly >> >> >> -- >> >> >> >> George McNamara, Ph.D. >> Single Cells Analyst >> L.J.N. Cooper Lab >> University of Texas M.D. Anderson Cancer Center >> Houston, TX 77054 >> http://works.bepress.com/gmcnamara/26/ |
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Craig Brideau |
Re: Direct diode modulation v aotf
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In reply to this post by Laevsky, Gary S.
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Your AOTF is more likely to experience problems than a modulated diode. The AOTF is dependent on a consistent RF tuning which can drift over time. Diodes on the other hand are dependent on their current feedback circuits. Older laser diodes did not have the benefit of superior current and temperature controls that are used now, so I agree that early generation units might suffer from modulation, but newer units should take it without complaint. There will be duty cycles and frequency regimes that will be easier on the diode, mostly for thermal reasons. This can be mitigated by the design of the package and electronics. Craig On 2013-10-17 12:39 PM, "Laevsky, Gary S." <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi All, > > It was always my impression that direct modulation of a diode shortened > the lifetime of said diode, vs pumping it at some constant voltage and > modulating through an aotf. > > Recently, I have been informed this is not the case, at least using the > OBIS LX lasers. > > Not that I would ever doubt anything I've ever been told, but a supporting > second opinion would be nice. > > Thanks. > > > Best, > > Gary > > > > Gary Laevsky, Ph.D. > Confocal Imaging Facility Manager > Dept. of Molecular Biology > Washington Rd. > Princeton University > Princeton, New Jersey, 08544-1014 > (O) 609 258 5432 > (C) 508 507 1310 > |
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