Dye Solution with Dipping lens

[Claire Brown]

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I have a user who would like to do a multiphoton experiment with dipping lenses with rhodamine dye in the dipping solution.
Would this be a problem for the dipping lenses?
I would assume as long as the lens is cleaned well after use it should be fine.

Anyone think otherwise?

Sincerely,

Claire

[Rosemary.White]

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Hi Claire,

It’s happened to us by accident! The lenses come up fine, though unless you clean very thoroughly, the ceramic tip may remain slightly pink.

cheers,
Rosemary

On 15/2/17, 2:20 pm, "Confocal Microscopy List on behalf of Claire Brown, Dr." <[hidden email] on behalf of [hidden email]> wrote:

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    I have a user who would like to do a multiphoton experiment with dipping lenses with rhodamine dye in the dipping solution.
    Would this be a problem for the dipping lenses?
    I would assume as long as the lens is cleaned well after use it should be fine.
   
    Anyone think otherwise?
   
    Sincerely,
   
    Claire
   

[Dan Focht]

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Claire


My main concern is the type of bond or sealant used to bond the lower element in the lens or the material the barrel of the lens tube is made of.
The bond or tube could have a surface porosity where dye could become bound.  
The glass will clean but residual dye elsewhere could be a problem.


Dan

> On Feb 14, 2017, at 10:20 PM, Claire Brown, Dr. <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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> *****
>
> I have a user who would like to do a multiphoton experiment with dipping lenses with rhodamine dye in the dipping solution.
> Would this be a problem for the dipping lenses?
> I would assume as long as the lens is cleaned well after use it should be fine.
>
> Anyone think otherwise?
>
> Sincerely,
>
> Claire

Dan Focht
Bioptechs, Inc.
3560 Beck Rd.
Butler PA 16002
V724-282-7145
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Toll Free 877 lIVE-CELL (548-3235)
[hidden email]

[Steffen Dietzel]

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Well, I used Hoechst once to stain nuclei in a tissue. Despite cleaning
of the dipping objective, we did see nuclear staining in subsequent
experiments. So yes, the lens may carry over a dye. Whether or not this
is a problem depends, of course. At the time it didn't bother us too
much and eventually the effect was lost.

It might help to keep the lens in a large water bath for some hours
(with flow?), to speed up the wash out.

Steffen


Am 15.02.2017 um 04:20 schrieb Claire Brown, Dr.:

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> To join, leave or search the confocal microscopy listserv, go to:
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> *****
>
> I have a user who would like to do a multiphoton experiment with dipping lenses with rhodamine dye in the dipping solution.
> Would this be a problem for the dipping lenses?
> I would assume as long as the lens is cleaned well after use it should be fine.
>
> Anyone think otherwise?
>
> Sincerely,
>
> Claire
>

--
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de

[mcammer]

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We've done it.  With rhodamine dextran.  No problems.  Recommend keeping lens hydrated between samples.

=========================================================================
 Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
         Cell:  914-309-3270     Office: Skirball 2nd Floor main office, back right
            http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Claire Brown, Dr.
Sent: Tuesday, February 14, 2017 10:20 PM
To: [hidden email]
Subject: Dye Solution with Dipping lens

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I have a user who would like to do a multiphoton experiment with dipping lenses with rhodamine dye in the dipping solution.
Would this be a problem for the dipping lenses?
I would assume as long as the lens is cleaned well after use it should be fine.

Anyone think otherwise?

Sincerely,

Claire

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[Craig Brideau]

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Some dyes can be cleared from the cone with a bath in vinegar if you notice
any persistence. I have used this for nile red.

Craig

On Feb 15, 2017 7:59 AM, "Cammer, Michael" <[hidden email]>
wrote:

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> We've done it.  With rhodamine dextran.  No problems.  Recommend keeping
> lens hydrated between samples.
>
> =========================================================================
>  Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical
> Center
>          Cell:  914-309-3270     Office: Skirball 2nd Floor main office,
> back right
>             http://ocs.med.nyu.edu/microscopy &
> http://microscopynotes.com/
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Claire Brown, Dr.
> Sent: Tuesday, February 14, 2017 10:20 PM
> To: [hidden email]
> Subject: Dye Solution with Dipping lens
>
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>
> I have a user who would like to do a multiphoton experiment with dipping
> lenses with rhodamine dye in the dipping solution.
> Would this be a problem for the dipping lenses?
> I would assume as long as the lens is cleaned well after use it should be
> fine.
>
> Anyone think otherwise?
>
> Sincerely,
>
> Claire
>
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