EMCCD read out speed

[Franziska Curdt]

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Hi,

I was wondering whether there is a way to shorten the read out speed of
my Andor IXON897 EMCCD camera. I am runnig it with a Labview program and
I cannot get faster than ~20ms per image, although I am only reading out one line of
512 pixels (1X512 Pixel image).

Settings:
Exposition time 0,5 ms
External Start Trigger in run till abort mode

Franziska

[Vitaly Boyko]

I am looking for a file converter plugin in Image J. In part
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Dear All,

I am looking for a file converter plugin in Image J. In particular, I would like to convert large >30GB 3D tile data set (ca. 4000x4000x300, xyz) from .lif to .ids/.ics (ideally ICS2) format. 

Thank you,

Vitaly
240-342-6217

[Cameron Nowell]

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Hi Vitaly,

There is a built in converter in Fiji (and I assume imageJ) but it won't do ICS format. But the LOCI tools can export to ICS (not ICS2 though). So you could write a simple macro to batch process a folder of files to convert them to ICS.

Do you lif files contain multiple experiments?

Cheers

Cam


Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research Melbourne - Parkville Branch
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vitaly Boyko
Sent: Friday, 17 August 2012 3:57 AM
To: [hidden email]
Subject: file converter plugin for Image J

I am looking for a file converter plugin in Image J. In part
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear All,

I am looking for a file converter plugin in Image J. In particular, I would like to convert large >30GB 3D tile data set (ca. 4000x4000x300, xyz) from .lif to .ids/.ics (ideally ICS2) format. 

Thank you,

Vitaly
240-342-6217

[Orla Hanrahan]

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Hi Franziska,

You should be able to achieve a faster readout than 20 ms if you read a row
rather than a column.  It seems that you are reading a 1 x 512 column which
means the whole sensor will be read out. However, if you choose a 512 x 1
row, your read out will be significantly reduced.

I hope this helps,

Orla Hanrahan,

Application Specialist,
Andor Technology