Bruno Pichler |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear list, Has anyone used EOPC's PLD-2SXY driver (http://www.eopc.com/driver_pld2sxy.html) to run two resonant scanners in phase-locked mode? I'm using their 8kHz SC-30 scanners, and they run in synch for maybe a minute or so, but then start to drift slowly out of phase. Has anyone else seen this behavior? Thanks, Bruno ______________________________________ Dr Bruno Pichler Independent NeuroScience Services INSS e: [hidden email] w: www.inss.org.uk |
Nino Karpf |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear Bruno, I cannot really help, just share that me made similar experiences. We experienced a phase drift even with a single resonant scanner from EOPC locked to a master frequency used for other components. Unfortunately, we didn’t find out how to get rid of this drift and EOPC could not help. We only got rid of the drift with an in-house built driver then. I would be interested to hear if you can resolve it. Good luck! Nino > Am 20.02.2021 um 10:20 schrieb Bruno Pichler <[hidden email]>: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear list, > > Has anyone used EOPC's PLD-2SXY driver (http://www.eopc.com/driver_pld2sxy.html) to run two resonant scanners in phase-locked mode? > I'm using their 8kHz SC-30 scanners, and they run in synch for maybe a minute or so, but then start to drift slowly out of phase. Has anyone else seen this behavior? > > Thanks, > Bruno > > > ______________________________________ > Dr Bruno Pichler > Independent NeuroScience Services INSS > e: [hidden email] > w: www.inss.org.uk |
In reply to this post by Bruno Pichler
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Bruno, I recently took over a project that uses an EOPC scanner. The previous person noticed that its phase drifts even for a single scanner. A solution that seemed to have worked for him was to keep the scanner running with the laser on for a few minutes before taking data. I haven't look into this issue deep enough but I guess it could be either caused by mechanical or laser heating. best, David -- David Chen, Ph.D. Bioimaging specialist at Rockefeller University |
Brian Patton |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thanks all for this conversation. This in interesting - are the phase drifts relative to the reference signal, or are you safe to ignore them once you keep proper track of the reference out from the driver? All the best, Brian -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of David C Sent: 20 February 2021 14:11 To: [hidden email] Subject: Re: EOPC resonant scanners in phase-lock mode CAUTION: This email originated outside the University. Check before clicking links or attachments. ***** To join, leave or search the confocal microscopy listserv, go to: https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Cbrian.patton%40STRATH.AC.UK%7C05f209b9ef1a4bd5efec08d8d5a98dc1%7C631e0763153347eba5cd0457bee5944e%7C0%7C0%7C637494271723849953%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=SjngQLPcCAPa9JJDV42a6VGxOGxYHESxCQHzlqnzqoc%3D&reserved=0 Post images on https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Cbrian.patton%40STRATH.AC.UK%7C05f209b9ef1a4bd5efec08d8d5a98dc1%7C631e0763153347eba5cd0457bee5944e%7C0%7C0%7C637494271723849953%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=ua6JhmUFXArl4uGIrDypbFhehDsmCBdzmH1Tp2hxwjY%3D&reserved=0 and include the link in your posting. ***** Hi Bruno, I recently took over a project that uses an EOPC scanner. The previous person noticed that its phase drifts even for a single scanner. A solution that seemed to have worked for him was to keep the scanner running with the laser on for a few minutes before taking data. I haven't look into this issue deep enough but I guess it could be either caused by mechanical or laser heating. best, David -- David Chen, Ph.D. Bioimaging specialist at Rockefeller University |
Bruno Pichler |
In reply to this post by Bruno Pichler
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi all, Thanks for the responses so far. I'll have a phone call with EOPC later today and I'll report back then. Best, Bruno -----Original Message----- From: Brian Patton <[hidden email]> Sent: 22 February 2021 09:55 Subject: Re: EOPC resonant scanners in phase-lock mode ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thanks all for this conversation. This in interesting - are the phase drifts relative to the reference signal, or are you safe to ignore them once you keep proper track of the reference out from the driver? All the best, Brian -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of David C Sent: 20 February 2021 14:11 To: [hidden email] Subject: Re: EOPC resonant scanners in phase-lock mode CAUTION: This email originated outside the University. Check before clicking links or attachments. ***** To join, leave or search the confocal microscopy listserv, go to: https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.e du%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Cbrian.patton% 40STRATH.AC.UK%7C05f209b9ef1a4bd5efec08d8d5a98dc1%7C631e0763153347eba5cd0457 bee5944e%7C0%7C0%7C637494271723849953%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLj AwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=SjngQL PcCAPa9JJDV42a6VGxOGxYHESxCQHzlqnzqoc%3D&reserved=0 Post images on https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.c om%2F&data=04%7C01%7Cbrian.patton%40STRATH.AC.UK%7C05f209b9ef1a4bd5efec0 8d8d5a98dc1%7C631e0763153347eba5cd0457bee5944e%7C0%7C0%7C637494271723849953% 7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwi LCJXVCI6Mn0%3D%7C3000&sdata=ua6JhmUFXArl4uGIrDypbFhehDsmCBdzmH1Tp2hxwjY% 3D&reserved=0 and include the link in your posting. ***** Hi Bruno, I recently took over a project that uses an EOPC scanner. The previous person noticed that its phase drifts even for a single scanner. A solution that seemed to have worked for him was to keep the scanner running with the laser on for a few minutes before taking data. I haven't look into this issue deep enough but I guess it could be either caused by mechanical or laser heating. best, David -- David Chen, Ph.D. Bioimaging specialist at Rockefeller University |
Bruno Pichler |
In reply to this post by Bruno Pichler
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** So, I spoke to Israel Tuchman at EOPC and it turns out that there is a gain setting somewhere in the synchronisation circuit that could possibly be tweaked to improve the phase drift. However, he was not confident that it would stabilise it enough for our application. Instead, we discussed the option of adding a DC command voltage input to the driver such that we can control the phase offset from software. This will hopefully allow us to apply some close-loop correction based on the image offset. It will take some time to send the driver back to EOPC and get this implemented, but I will let you know if and when I get it to work. -----Original Message----- From: Bruno Pichler <[hidden email]> Sent: 23 February 2021 10:22 Subject: Re: EOPC resonant scanners in phase-lock mode ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi all, Thanks for the responses so far. I'll have a phone call with EOPC later today and I'll report back then. Best, Bruno -----Original Message----- From: Brian Patton <[hidden email]> Sent: 22 February 2021 09:55 Subject: Re: EOPC resonant scanners in phase-lock mode ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thanks all for this conversation. This in interesting - are the phase drifts relative to the reference signal, or are you safe to ignore them once you keep proper track of the reference out from the driver? All the best, Brian -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of David C Sent: 20 February 2021 14:11 To: [hidden email] Subject: Re: EOPC resonant scanners in phase-lock mode CAUTION: This email originated outside the University. Check before clicking links or attachments. ***** To join, leave or search the confocal microscopy listserv, go to: https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.e du%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Cbrian.patton% 40STRATH.AC.UK%7C05f209b9ef1a4bd5efec08d8d5a98dc1%7C631e0763153347eba5cd0457 bee5944e%7C0%7C0%7C637494271723849953%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLj AwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=SjngQL PcCAPa9JJDV42a6VGxOGxYHESxCQHzlqnzqoc%3D&reserved=0 Post images on https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.c om%2F&data=04%7C01%7Cbrian.patton%40STRATH.AC.UK%7C05f209b9ef1a4bd5efec0 8d8d5a98dc1%7C631e0763153347eba5cd0457bee5944e%7C0%7C0%7C637494271723849953% 7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwi LCJXVCI6Mn0%3D%7C3000&sdata=ua6JhmUFXArl4uGIrDypbFhehDsmCBdzmH1Tp2hxwjY% 3D&reserved=0 and include the link in your posting. ***** Hi Bruno, I recently took over a project that uses an EOPC scanner. The previous person noticed that its phase drifts even for a single scanner. A solution that seemed to have worked for him was to keep the scanner running with the laser on for a few minutes before taking data. I haven't look into this issue deep enough but I guess it could be either caused by mechanical or laser heating. best, David -- David Chen, Ph.D. Bioimaging specialist at Rockefeller University |
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